Adavanced Breeding Methods for quailty improvement in solanaceous- Pallavi WaniPallavi Wani
1. Welcome
2.Back ground information
9. Vegetable crops are rich in dietary micronutrients, antioxidants and functional phytochemicals. Among vegetables solanaceous vegetables are the good source of health- protective dietary elements such as vitamins, minerals and antioxidant in the human diet. These solanaceous family includes Tomato, Brinjal, Chilli and Potato. (Kumari et al., 2017)
11. Advantages of Quality Breeding
14.Quality traits of Solanaceous vegetables
20. Mutation Breeding
23. Case study
28. Molecular Breeding
30. case study
39. Marker assisted selection
40.Steps in Marker Assisted Selection
41. Marker Assisted-Backcrossing (MAB)
56. Genetic Engineering-It refers to direct or artificial manipulation of one or many genes, most often foreign gene is inserted into an organism to get a desired phenotype. Ex: Golden rice, Flavr savr tomato etc.,
Steps involved in genetic engineering are
1. Extraction of plasmid from bacterial cell which is followed by isolation our gene of interest
2. After that cutting DNA at specific location with help of certain enzymes called restriction enzymes or molecular scissors
3. After the second step that is cutting DNA segment followed by the ligases process it is nothing but joining of DNA fragments of desired sequences along with plasmid molecule to form a specific type of hybrid DNA is called recombinant DNA technology
4. In next step the rDNA inserted into the host cell to form a transformed bacterial cell these transformed bacterial cell allowed them to grow inside a media
5. Last step is selection and screening of transformed cells with desired character.
63. RNAi-RNA interference technology (RNAi): sequence specific gene silencing phenomenon caused by the presence of double stranded RNA. These RNAi allows down regulation in gene in a more precise manner without effecting expression of other genes. It was first observed by Richard in 1990.
67. Components of RNA interferance
70. CRISPR-It has been successfully applied to various plants, including Rice, Wheat, Maize and Tomato (clustered regularly interspaced short palindromic repeats) is a types of DNA sequences found in the genomes of prokaryotic organisms such as bacteria. It is nothing but acquired immune system present in prokaryotes, which is identified in early 1987 in E. coli.Later, scientists identified and modified as a powerful gene editing tool and got a broad application in genome research field due to its ease of use and cost-effectiveness. Emmanuelle Charpentier and Jennifer Doudna got nobel prize for genome editing discovery.
78. Case study on CRISPR- Potato
84. Achivements
85. Wild Sources for quality traits
86. Varieties released with improved quality traits.
87. Transgenic Approach for Bio fortification in vegetables crops-Biofortification refers to process of improving nutritional quality of food crops
90. CONCLUSION
Urban farming mencakup beberapa sektor di antaranya pertanian, perternakan, dan perikanan di kesempatan kali ini akan menjelaskan urban farming di sektor pertanian.
Adavanced Breeding Methods for quailty improvement in solanaceous- Pallavi WaniPallavi Wani
1. Welcome
2.Back ground information
9. Vegetable crops are rich in dietary micronutrients, antioxidants and functional phytochemicals. Among vegetables solanaceous vegetables are the good source of health- protective dietary elements such as vitamins, minerals and antioxidant in the human diet. These solanaceous family includes Tomato, Brinjal, Chilli and Potato. (Kumari et al., 2017)
11. Advantages of Quality Breeding
14.Quality traits of Solanaceous vegetables
20. Mutation Breeding
23. Case study
28. Molecular Breeding
30. case study
39. Marker assisted selection
40.Steps in Marker Assisted Selection
41. Marker Assisted-Backcrossing (MAB)
56. Genetic Engineering-It refers to direct or artificial manipulation of one or many genes, most often foreign gene is inserted into an organism to get a desired phenotype. Ex: Golden rice, Flavr savr tomato etc.,
Steps involved in genetic engineering are
1. Extraction of plasmid from bacterial cell which is followed by isolation our gene of interest
2. After that cutting DNA at specific location with help of certain enzymes called restriction enzymes or molecular scissors
3. After the second step that is cutting DNA segment followed by the ligases process it is nothing but joining of DNA fragments of desired sequences along with plasmid molecule to form a specific type of hybrid DNA is called recombinant DNA technology
4. In next step the rDNA inserted into the host cell to form a transformed bacterial cell these transformed bacterial cell allowed them to grow inside a media
5. Last step is selection and screening of transformed cells with desired character.
63. RNAi-RNA interference technology (RNAi): sequence specific gene silencing phenomenon caused by the presence of double stranded RNA. These RNAi allows down regulation in gene in a more precise manner without effecting expression of other genes. It was first observed by Richard in 1990.
67. Components of RNA interferance
70. CRISPR-It has been successfully applied to various plants, including Rice, Wheat, Maize and Tomato (clustered regularly interspaced short palindromic repeats) is a types of DNA sequences found in the genomes of prokaryotic organisms such as bacteria. It is nothing but acquired immune system present in prokaryotes, which is identified in early 1987 in E. coli.Later, scientists identified and modified as a powerful gene editing tool and got a broad application in genome research field due to its ease of use and cost-effectiveness. Emmanuelle Charpentier and Jennifer Doudna got nobel prize for genome editing discovery.
78. Case study on CRISPR- Potato
84. Achivements
85. Wild Sources for quality traits
86. Varieties released with improved quality traits.
87. Transgenic Approach for Bio fortification in vegetables crops-Biofortification refers to process of improving nutritional quality of food crops
90. CONCLUSION
Urban farming mencakup beberapa sektor di antaranya pertanian, perternakan, dan perikanan di kesempatan kali ini akan menjelaskan urban farming di sektor pertanian.
4. RUMUSAN MASALAH
Apa itu lalat buah ?
Apa dampak yang ditimbulkan oleh lalat
buah terhadap hasil panen ?
Bagaimana cara pengendalian lalat buah ?
21/11/2014