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1. XBT602 Process Biotechnology-Upstream
Oxygen Mass Transfer Co-efficient by Various
Methods
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Present By
Abirami V (121011101409)
Vaishnavi P ( 121011101434)
B. Tech Biotechnology
III year
Submitted to
Ms. P. Mala
Assistant Professor
Department of Biotechnology
Periyar Maniammai Institute of Science & technology

2. Definition of Oxygen Mass Transfer Coefficient (OMTC):
OMTC refers to the efficiency of oxygen transfer from one phase
to another within a given system, crucial for processes like
fermentation, wastewater treatment, and pharmaceutical
production.
Significance:
OMTC measurement allows industries to optimize oxygen
transfer, leading to improved product yield, quality, and energy
efficiency.
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3. Explanation:
OMTC quantifies the rate at which oxygen is transferred across phase
boundaries, providing insights into mass transfer phenomena in various
industrial processes.
Importance:
Accurate OMTC measurement aids in process optimization, ensuring optimal
oxygen levels for microbial growth, chemical reactions, and product
formation.
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4. Measurement Methods
Dynamic Gassing Out Method:
Continuous sparging of gas into a liquid with measurement of dissolved oxygen
concentration over time.-
Static Headspace Method:
Equilibration of gas phase above a liquid sample with subsequent measurement of gas
composition.
Membrane Introduction Mass Spectrometry (MIMS):
Direct measurement of dissolved gases using a semipermeable membrane and
mass spectrometry.
Electrochemical Sensors:
Detection of dissolved oxygen through electrochemical reactions at an electrode.
Optical Sensors:
Indirect measurement of dissolved oxygen using light absorption or fluorescence
properties.
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5. Dynamic Gassing Out Method
Description:
Involves continuous gas sparging into a liquid with simultaneous
measurement of dissolved oxygen concentration.-
Principle:
OMTC is calculated from the rate of change of dissolved oxygen
concentration over time.- Applications: Widely used in bioreactors
for monitoring oxygen transfer rates during microbial fermentation
processes.
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6. Static Headspace Method
Description:
Gas-phase equilibrium is established above a liquid sample, followed by
measurement of gas composition.-
Differentiation:
Contrasts with the dynamic method by relying on phase equilibrium rather
than continuous gas sparging.
Advantages and Limitations:
Offers simplicity but may not accurately represent dynamic oxygen transfer
conditions.
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7. Membrane Introduction Mass Spectrometry (MIMS)
Description:
Direct measurement of dissolved gases using a semipermeable
membrane and mass spectrometry.-
Principle:
Gas diffusion through the membrane into a mass spectrometer for
analysis.
Advantages:
High sensitivity and real-time measurement capabilities, suitable
for studying dynamic oxygen transfer processes.
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8. Electrochemical Sensors
Description:
Detection of dissolved oxygen through electrochemical reactions
at an electrode.
Principle:
Oxygen reduction reactions generate an electrical signal
proportional to oxygen concentration.
Advantage
Rapid response times and good sensitivity but may require
calibration and maintenance.
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9. Optical Sensors
Description:
Indirect measurement of dissolved oxygen using light absorption or
fluorescence properties.
Principle:
Oxygen-dependent quenching of luminescent probes or absorption
of oxygen-sensitive dyes.
Advantages and Limitations:
Non-invasive measurement capabilities but may be susceptible to
interference.
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10. Factors Influencing OMTC
Factors affecting OMTC measurements, such as
temperature, pressure, agitation, and dissolved solids
content.
Understanding and controlling these factors are
essential for accurate OMTC measurement.
• Gas Bubbles size
• Air flow rate
• Properties of the liquid and medium
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11. ADVANTAGE
• The mass transfer coefficient advantage refers to the efficiency
with which mass transfer occurs between phases in a system,
such as between a gas and a liquid.
• A higher mass transfer coefficient means that mass transfer
happens more rapidly, leading to quicker equilibration or reaction
times.
• This advantage is crucial in various industrial processes like
distillation, absorption, and stripping, where optimizing mass
transfer can significantly enhance productivity and cost-
effectiveness.
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12. APPLICATIONS:
Mass transfer coefficient, often denoted as ( k ), is a critical parameter in various
engineering and scientific applications, particularly in fields like chemical engineering,
environmental engineering, and biotechnology. Some common applications include:
1. Chemical Engineering: In chemical reactors, mass transfer coefficients determine the
rate of chemical reactions by governing the transport of reactants to the reaction sites.
This is crucial for optimizing reactor design and improving reaction efficiency.
2. Separation Processes: Mass transfer coefficients are essential in processes such as
distillation, absorption, and extraction. They dictate the rate at which components are
transferred between phases, facilitating the separation of mixtures.
3. Environmental Engineering: Mass transfer coefficients play a key role in processes
like air pollution control, where they govern the transfer of pollutants between the air
and other media (e.g., water, soil) in processes like absorption or adsorption.
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1. Which method involves continuous sparging of gas into a liquid with simultaneous measurement of dissolved oxygen
concentration?
a) Static Headspace Method
b) Membrane Introduction Mass Spectrometry (MIMS)
c) Dynamic Gassing Out Method
d) Optical Sensors
2.Which technique utilizes electrochemical reactions at an electrode to detect dissolved oxygen concentrations?
a) Dynamic Gassing Out Method
b) Static Headspace Method
c) Electrochemical Sensors
d) Membrane Introduction Mass Spectrometry (MIMS)
3.Which method relies on establishing gas-phase equilibrium above a liquid sample for measurement?
a) Dynamic Gassing Out Method
b) Static Headspace Method
c) Optical Sensors
d) Membrane Introduction Mass Spectrometry (MIMS)

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4.Which technique offers high sensitivity and real-time measurement
capabilities for studying dynamic oxygen transfer processes?
a) Dynamic Gassing Out Method
b) Electrochemical Sensors
c) Membrane Introduction Mass Spectrometry (MIMS)
d) Optical Sensors
5. What is the primary purpose of measuring Oxygen Mass Transfer Coefficient
(OMTC) in various industries?
a) To determine the density of oxygen in a given medium
b) To optimize oxygen transfer processes and improve product yield
c) To calculate the rate of oxygen consumption in chemical reactions
d) To monitor the concentration of dissolved oxygen in wastewater

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Here are the correct answers to the multiple-choice questions:
1.Correct Answer: c) Dynamic Gassing Out Method
2. Correct Answer: c) Electrochemical Sensors
3. Correct Answer: b) Static Headspace Method
4. Correct Answer: c) Membrane Introduction Mass Spectrometry
(MIMS)
5. Correct Answer: b) To optimize oxygen transfer processes and
improve product yield

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Thank you