1. The document discusses characterizing seeding conditions for image velocimetry techniques used with unmanned aerial systems (UASs) for hydrological monitoring. 2. Field experiments were conducted using artificial tracers deployed via UAS in three rivers to quantify seeding characteristics. Metrics for seeding density, spatial distribution, and tracer size variation were statistically analyzed. 3. Results showed the seeding metrics had a significant impact on the accuracy of surface velocity estimates from particle tracking velocimetry and laser speckle pattern interferometry techniques, with density and distribution most influential. Proper seeding characterization could help optimize image analysis.