1. Curriculum Vitae
Name: Sanjay Kumar Hirasker E-Mail: sanjay.hirasker@gmail.com
Contact: +91-9550269956
Profile Synopsis
E.I. DuPont India Pvt. Ltd.: (Dec 2013 to Present) currently working as Research Associate on Yeast
metabolic engineering platform.
Evolva Biotech Pvt. Ltd based in Chennai, India (a subsidiary of Evolva Holdings, Swiss): (Nov 2007 to Nov
2013) worked in Cell and molecular biology department for 6 years as Research Scientist.
Professional Synopsis
1 A dynamic professional with 8+ years of rich Industrial experience in Research & Development in a
synthetic biology with primary focus on basic research which discovers and provides innovative, high
value, sustainable ingredients – particularly for health, wellness and nutrition.
2 Cell biology: Development, optimization and screening of various cell based assays utilizing mammalian
cell lines.
3 Molecular biology: Thorough knowledge on cloning, expression of recombinant proteins in Yeast,
Bacteria and mammalian cell line culture.
4 Yeast metabolic engineering: Stable integration of heterologous pathways in Yeast and optimization
studies.
5 Analytical skills: Hands on experience in analyzing invitro and fermentation samples by HPLC to
determine metabolic flux (independent operations).
6 A rich experience in finding out missing enzymes of metabolic pathways which has economic significance
in the area of nutrition from pyrosequenced data.
7 An effective communicator with excellent relationship management skills and strong analytical, problem
solving & organizational abilities. Flexibility to work in any area which has great impact in improving lives
of common people.
Scholastics
Post-Graduation (M.Sc. Microbiology)
Osmania University, Hyderabad – Batch 2007. Secured 70.1% marks. (Secured 4th
place in the entire batch)
Graduation (B.Sc. - Microbiology / Zoology / Chemistry)
Osmania University, Hyderabad - 2005. Secured 68.9% marks. Stood 1st
(Awarded Gold medal)
Board of Intermediate Education 10+2 (Biology / Physics / Chemistry)
Govt. Junior College, Hyderabad - 2002. Secured 71.6% marks. (Secured 1st
place in the entire batch)
SSC-10TH
Standard
Mufeed-Ul-Anam Boys High School, Hyd- 2000. Scored 69.3% marks. (Secured 1st
place in the entire batch)
Technical Expertise –Industrial Experience
Research & Development: Cell and molecular biology:
 Mammalian Cell line: Development of replicon model assay system for alpha-like virus class in
mammalian cell line (HEK-293) which has relevance with Dengue virus, Chikungunya virus and HCV
replication (+ve standed RNA virus).
 Identification of small molecule based immunostimulators that activate Toll like receptor – Development
and high throughput screening of compounds through TLR8-SEAP Activation Assay in HeLa cell line.
 Reporter assay development for screening compounds against NDM-1 enzyme target in mammalian cells.

2.  Assay validation and screening of compounds on various target based mammalian in-vitro assay system
(HeLa, BHK-21, RAW 264.7) in 96 well formats.
 Yeast metabolic engineering – Stable integration of heterogeneous pathways in Yeast to produce
commercially significant flavor, fragrance (F & F) and nutraceutical related molecules.
 Development of knock-out strains of Yeast for pathway optimization.
 Highly experienced in assay development, optimization & screening of small molecules on Yeast based in-
vitro assays like FRET signal detection systems for protein-protein interactions for anti-HIV and anti-
Ebola viral drug discovery programmes.
 Worked on drug discovery programmes on Tuberculosis, anti-dandruff and melioidosis. Validation and
high-throughputisation of compound screening on Mycobacterium, Malassezia and Burkholderia strains
in different well formats.
 Unknown enzyme identification and pulling out genes from pyro-sequenced data for mode of action
studies and functionality check.
 Invitro transcription and translation of various proteins (enzymes) for testing the expression and
functionality of the genes.
 Cloning, expression of various recombinant proteins in Bacteria, Yeast and Mammalian cells.
Wet lab Operations
 Well experienced in handling various mammalian cell lines.
 Various Yeast and mammalian cell based assay screening.
 Testing inherent cyto-toxicity (by XTT assay methodology) on mammalian cell lines.
 Well versed in all the molecular biology and cell culture techniques.
 Study of genes involved in the plant resistance against pathogenesis.
 Work experience in handling human pathogen (Mycobacterium tuberculosis) in one of the nation’s best
containment facility of BSL 3.
Documentation
 Follow all the standard international regulatory norms - ISO 9001-2008.
 Electronic Data integration system of results obtained from all the experiments.
 Ensuring compliance of various quality measures by maintaining requisite documentation / records.
 Preparing periodic laboratory reports for information, compliance & corrective action.
Technical Proficiency
1 Biochemical Techniques
 Biochemical estimations, in-vitro Enzyme Assays, HPLC & Spectroscopic Techniques.
2 Molecular Biology & Immunological Techniques
 USER and Infusion cloning methodology, In vivo (Conventional recombination) ligation methodology in
Yeast, In vitro transcription and translation-cell free assays, Electrophoresis, SDS-PAGE, Western blots,
Restriction digestion, Nucleic Acid Isolation, ELISA, DIG labelled probing and various Ag-Ab reaction
tests, Multiplex and RACE PCR, TA cloning, Cloning and expression studies of recombinant fusion protein
in E. coli.
 Insilico genetic data analysis skills.
3 Microbiological/Cell culture Techniques
 Bacterial/Yeast Transformation & all other microbiological and cell culture techniques, transfection
studies.
 In-vitro anti-bacterial and anti-fungal assay in high throughput format (96 well and 384 well plate)
 Bio analytical Techniques: Complete HPLC operation and analysis and interpretation of data.

3. Conferences/Meetings/Workshops attended
 Poster presentation on “Development of on mammalian cell NDM-1 reporter assay development for
screening compounds against NDM-1 enzyme target” and got selected for Deakin University Ph.D.
programme.
 Attended hands-on training on basics of lab scale bioreactor operations in Biozeen, Bangalore.
 participated in a lecture workshop in modern biology ’’BIO-LOGIKS’’ from Molecules to processes under the
aegis of Indian Academy of Sciences, Bangalore and University of Hyderabad.
 Poster presentation on ‘’Proteases’’ in annual lecture series ‘’Gyana Tarangini Vyakhyaana Mala’’
conducted by Bharatiya Vidya Bhavan’s College and our poster was awarded as best poster.
Field of Interest
 Metabolic engineering in Yeast or any related organisms.
 Drug discovery in the field of Infectious diseases especially the area of neglected diseases.
 Has flexibility to work in any area which has great impact on improving lives of common people.
Personal Portfolio
Date of Birth: 19th
of November 1984
Permanent Address: H.No: 8-12-96/R/52, Rajiv shetty Nagar, Karmanghat road, Hyderabad, A.P. PIN 500097
Linguistic Abilities: English / Hindi / Telugu / Marathi
References: Provided upon request (as separate attachment)
Declaration:
I hereby declare that the above information is true to the best of my knowledge.
Place: Hyderabad, India
Date: 25/01/2016 (SANJAY KUMAR)