I would describe myself as a conscientious worker, problem solver and a dependable team player with laboratory expertise in molecular cloning, virology and immunology. I am proficient at developing small animal infection models, mammalian cell culture, proficient at working in Biosafety level -2 and 3 laboratories, developing recombinant gene constructs through primer design, restriction digestion and bacterial cloning. I am proficient at nucleic acid (DNA/RNA) extraction, quantitative PCR and RT-PCR. My technical expertise in virology and immunology include performing plaque assays and foci forming assays to quantify viremia and ELISA. I possess extensive training in working with laboratory animals and I am proficient at intra-nasal and intra-peritoneal drug administration, working with animal restraint systems like the In-TOX system and have performed nebulization studies using guinea pigs in the past. I also perform tail DNA genotyping in mice.

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SHRAVIDA SHETTY
shravida.shetty@ucdenver.edu / shravida@gmail.com Tel: 7203691953
SUMMARY
A biomedical science postgraduate with laboratory work experience and technical expertise in molecular
biology, virology, mammalian cell culture and immunology techniques seeks a suitable role in biosciences.
PROFESSIONAL EXPERIENCE
Graduate Student – Dr. Mario Santiago laboratory, University of Colorado, Aurora (August 2015 –
Present)
Research Intern – Dr. Rytis Prekeris laboratory, University of Colorado, Aurora (April 2013 - May 2014)
Research Associate – Piramal Lifesciences Limited, Mumbai, India (January 2011 – December 2012)
Medical Laboratory assistant – Kingston Hospital NHS trust, Surrey, United Kingdom (August 2008 –
October 2010)
SKILLS & WORK EXPERIENCE
Graduate Student – Dr. Mario Santiago laboratory, University of Colorado, Aurora
 Proficient in molecular cloning techniques like developing recombinant gene constructs, DNA and RNA
isolation, quantifying plasma viral loads and proviral DNA using quantitative PCR and RT-PCR, gene
sequence analysis
 BSL-2 experience - Mammalian cell culture (muss dunni, 293 and 3T3 cell lines), preparing Friend
virus stocks, quantifying viremia using plaque assay and foci forming assays
 Flow cytometry (FACS DIVA II), ELISA assays, SDS PAGE and Western blotting
 Laboratory animal work - Mice genotyping using tail DNA, tissue (spleen and bone marrow) extraction
and cardiac punctures
Research Volunteer - Dr. Rytis Prekeris laboratory, University of Colorado, Aurora
 Performed lentiviral shRNA transduction, protein expression and purification, mammalian cell culture
and fluorescent staining techniques using FITC and DAPI
 Soft Agar assays in order to detect tumorigenic cellular transformation
 HELA cell culture
Research Associate – Piramal Lifesciences Limited, Mumbai, India
 Design and execution of pre-clinical trials to test the anti-tubercular activity of an investigational new
drug (IND) molecule
 Biosafety level 3 laboratory work experience – Preparation of mycobacterium growth media, Acid fast
staining, sub – cultured multidrug resistant Mycobacterium.tuberculosis
 Laboratory animal work – Developed tuberculosis animal infection models, animal tissue extraction,
intra-nasal, intra-peritoneal and oral drug administration

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 Laboratory management – Supervision of the animal facility staff, performed QA/QC checks in the
BSL-3, managed laboratory inventory and equipment maintenance schedules, maintained SOPs
Medical Laboratory Assistant - Kingston Hospital NHS trust, Surrey, United Kingdom
 Follow GMP lab practices, data entry into LIMS (Laboratory Information Management System
softwares)
 Microscopy, Gram’s staining and patient MRSA nasal swab and High vaginal swab test
 Prepared microbiological media and practiced aseptic microbiological techniques
 Detection of bacteremia in patient blood samples using blood culture systems like BACTEC
EDUCATIONAL QUALIFICATIONS
MSc. Biomedical Basic Sciences August 2014 - 2016 (expected)
University of Colorado, Aurora, United States of America
BSc. Biomedical Science July 2004 – 2008
University of Central Lancashire, Preston, United Kingdom
ADDITIONAL SKILLS & ATTRIBUTES
 Highly Adaptable – Worked in 3 different countries, worked in diagnostics, industry and academic
research
 Excellent team member – Have been acknowledged as a conscientious, dependable co-worker by
past and present fellow team members
 Computer Skills - Proficient in MS Word, Excel and Powerpoint, Prism, FLOWJO (Flow cytometry
analysis software) and digital tools used for molecular cloning like MacVector, Sequencher and
Expassy
 Currently, volunteering as an English tutor at the local county library (Douglas County libraries)
REFERENCES
1. Dr. Mario Santiago, Associate Professor, Division of Infectious Diseases, University of Colorado,
Denver
Tel: 303-724-4946
Email: mario.santiago@ucdenver.edu
2. Dr. Rytis Prekeris, Associate Professor, Department of Cell and Developmental Biology, University
of Colorado, Denver
Tel: 303-724-3410
Email: Rytis.Prekeris@ucdenver.edu
3. Tiffany Curtin, Literacy Specialist, Douglas County Libraries, Philip S Miller 100S. Wilcox, Castle
Rock, CO-80104, Tel: 303-688-7646, Email: tcurtin@dclibraries.org