Biochemistry of milk & milk products
milk definition,biochemical aspects,flavor,nutritional aspects,description of various milk products ( fermented & non fermented)
Milk is a unique in that it is both consumed, as fluid milk with minimal processing and it is the raw material used to manufacture a wide variety of product.
Different Platform Tests for Raw Milk at Raw Milk Reception Dock(RMRD)sunil meena
Raw milk has to pass through rigorous examination which may include organoleptic,physical and chemical tests to assess the quality of intake milk rapidly, and decide for its acceptance or rejection. All these tests known as “platform tests” are performed on each can/tanker to assess the quality of the incoming milk before it is accepted and weighed. These tests must be easy to perform, give quick and reliable results and should not require complicated and elaborate equipment. The classification of milk on the basis of quality is usually referred to “grading of milk”.So grading of milk is done on the basis of platform tests which include organoleptic
as well as preliminary tests.The milk is collected from various sources and transported to milk scheme for processing, marketing and distribution. Large quantity of milk is supplied to the plant through different agencies, so that is subjected to check for its suitability. Hence it is essential to examine the milk by using different platform tests like organoleptic evaluation (OE), Clot on boiling test (COP), Alcohol test (AT), Sediment test (ST), Resazurin test (RT).
Milk
Composition of milk
physical properties of milk
Nutritive value of milk
Milk processing
Packaging of milk
Cream
Physico-chemical properties of cream
Butter
Process of butter making
Channa
Chhena or sana are curds or cheese curds, originating from the Indian subcontinent. Chhana is an acid coagulated product obtained from milk.
Paneer
Paneer is a heat-acid coagulated milk product obtained by coagulating standardized milk with the permitted acids at specified temperature
Chemical Composition
The chemical composition of chhena depends mainly on the initial composition of milk, the conditions of coagulation, the technique of straining( which determines the moisture content), and loses of milk solids in the whey.
Milk is a unique in that it is both consumed, as fluid milk with minimal processing and it is the raw material used to manufacture a wide variety of product.
Different Platform Tests for Raw Milk at Raw Milk Reception Dock(RMRD)sunil meena
Raw milk has to pass through rigorous examination which may include organoleptic,physical and chemical tests to assess the quality of intake milk rapidly, and decide for its acceptance or rejection. All these tests known as “platform tests” are performed on each can/tanker to assess the quality of the incoming milk before it is accepted and weighed. These tests must be easy to perform, give quick and reliable results and should not require complicated and elaborate equipment. The classification of milk on the basis of quality is usually referred to “grading of milk”.So grading of milk is done on the basis of platform tests which include organoleptic
as well as preliminary tests.The milk is collected from various sources and transported to milk scheme for processing, marketing and distribution. Large quantity of milk is supplied to the plant through different agencies, so that is subjected to check for its suitability. Hence it is essential to examine the milk by using different platform tests like organoleptic evaluation (OE), Clot on boiling test (COP), Alcohol test (AT), Sediment test (ST), Resazurin test (RT).
Milk
Composition of milk
physical properties of milk
Nutritive value of milk
Milk processing
Packaging of milk
Cream
Physico-chemical properties of cream
Butter
Process of butter making
Channa
Chhena or sana are curds or cheese curds, originating from the Indian subcontinent. Chhana is an acid coagulated product obtained from milk.
Paneer
Paneer is a heat-acid coagulated milk product obtained by coagulating standardized milk with the permitted acids at specified temperature
Chemical Composition
The chemical composition of chhena depends mainly on the initial composition of milk, the conditions of coagulation, the technique of straining( which determines the moisture content), and loses of milk solids in the whey.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
2. MILK
No other single food has as many nutritive value as milk.
It contains nearly all the nutrients necessary to sustain life.
Its ideal food for infants & growing child
Milk is a white or yellow-white, opaque liquid
The color is influenced by scattering and absorption of light by milk fat
globules and protein micelles.
Milk tastes mildly sweet, while its odor and flavor are normally quite
faint.
Main structural elements of milk are fat globules , casein micelles ,
globular proteins, lipoproteins , etc.
3. COMPOSITION OF MILK
WATER:- 87.88%
CARBOHYDRATES:-(approx. 5%)
• mainly lactose
•In heated milk products (condensed milk) there is lactulose which is
little sweeter
4. FATS:- 3-4%
• Contains fat soluble vitamins ,pigments, carotenes, xanthophylls, cholesterol &
phospholipids
• Fat in the milk occurs in the form of droplets or globules,surrounded by membrane and
emulsified in milk serum
• Milk is an oil in water(o/w) emulsion which is not naturally physically stable that is why
creaming occurs if it is left to stand.
PROTEINS:-3-4%
• Milk consists of 80% casein & 20% whey proteins.
• The casein is arranged in super-structures called micelles, which consist of protein
with phosphate, citrate and calcium.
• The caseins are actually a group of similar proteins, which can be separated from the
milk proteins by acidification to a pH of 4.6
• The casein micelles also may be coagulated by addition of the enzyme rennin.
CASEIN:-
5. lactalbumin, lactoglobulin & immunoglobulin
Whey proteins are more hydrated than casein and are denatured and precipitated by heat
rather than by acid.
include enzymes such as lipase, protease, & alkaline phosphatase, which hydrolysesTGs,
proteins, & phosphate esters, respectively.
The milk proteins are of high BV & are readily used by the body
WHEY PROTEINS:-
OTHER PROTEIN
COMPONENTS:-
VITAMINS:-
• Milk consists ofVitaminsA, B6, B12, C, D, K, E, thiamine, niacin, biotin, riboflavin, folates,
and pantothenic acid.
• Whole milk containsVitamin A naturally & fortified with vitamin D.
• Low-fat & non-fat milks are fortified with both vitaminA & D.
MINERALS:-
• Ca & P approx. 1% of milk
• Ca is present as calcium caseinate, calcium phosphate & calcium citrate.
• Other minerals present are chloride, magnesium, potassium, sodium, and sulfur.
7. NUTRITIVE VALUE
Good quality protein & BV is over 90%. Lysine is abundance.
Easily digestible fat containing 2.1% linoleic acid, 0.5% linolenic acid & 0.14%
Arachidonic acid.
Only substance that contain lactose which is essential for:
o Synthesis of myelin sheath(galactose)
o Favors the growth of lactobacillus in intestine & decrease the pH thus favoring Ca
absorption.
o Also increases the permeability of Small Intestine for Ca+2 .
Poor source of iron & vit C.
Not a good source of niacin
but excellent source of tryptophan.
Major source of Calcium & riboflavin.
8. FLAVOR OF MILK
The flavour of milk is mild & slightly sweet.The characteristic mouth-feel is due to the presence
of emulsified fat, colloidally dispersed proteins, the carbohydrate lactose, & milk salts.
Fresh milk contains acetone, acetaldehyde, methyl ketones, & SFAs that provide aroma.
“Flavour treatment” to standardize the odor and flavor typically follows pasteurization. In this
treatment process, milk is instantly heated to 195°F (91°C) with live steam (injected directly into
the product), and subsequently subject to a vacuum that removes volatile off- flavors and
evaporates excess water produced from the steam.
Lipase activity causes rancidity of the fat, unless destroyed by the heat of pasteurization. (Or,
the short-chain butyric acid may produce an off- odor or off- flavour due to bacteria rather than
lipase in the emulsified water of milk.).
Light-induced flavour changes in the proteins and riboflavin because vitamin B2 acts as
photosynthesizer .
9. MILK PRODUCTS
BUTTER:-
fat content is generally about 80%.
Made from sweet or sour cream.
Butter is a concentrated form of fluid milk produced through churning of pasteurized
pasteurized cream. Churning involves agitation that breaks fat globule membranes so
the emulsion breaks, fat coalesces, and water (buttermilk) escapes.
The original 20/80 oil-in-water type of emulsion of milk becomes a 20/80 water-in-oil
emulsion.
Milk is churned to form butter and the watery buttermilk. Butter may have a yellow color
due to the fat-soluble animal pigment, carotene, or an additive.
Butter spoil as a result of hydrolysis ofTG molecules releasing free
butyric & caproic acids.
10. GHEE:-
a type of clarified butter
prepared by simmering butter and removing the residue.
The texture, colour , and taste of ghee depends on the quality of the butter and
the duration of the boiling.
BUTTERMILK:-
liquid left behind after churning butter out of cream.
It is beneficial to health as it contains probiotic microbes
fat content of buttermilk is far lower than milk or curd.
11. CURD:-
prepared by cooling boiled milk to body temperature & adding 5-10%
starter. After 6-8 hours an acidity of 0.9-1% is formed which coagulate
casein & curd is set.
Easily digested than normal milk.
Contains more vitamin B than milk.
Used as marinating & souring agent in cookery.
YOGURT:-
Yogurt is a variety of curd. Whole, low fat, skim milks & even cream can
be used to make yogurt.
In production of yogurt, a mixed culture of Streptococcus thermophilus,
Lactobacillus acidophilus is usually added to the pasteurized milk &
incubated at 42-46°C.
Increase in folic acid concentration during fermentation.
Fermented milk is useful for a wide variety of disorders like colitis,
constipation, diarrhoea, gastroenteritis, diabetes & hyper cholesteremia..
12. SHRIKHAND:-
fermented product made by concentrating dahi by removing whey &
to which sugar, flavor & condiments are added.
Shrikhand is an Indian sweet dish made of strained dahi.
CHEESE:-
Cheese is made up of casein by coagulation.
Varieties of cheese are differentiated according to their
*Flavor *Salts & seasoning added
*Texture *Manufacturing & processing method
*Type of milk *Type of bacteria & mould species used in ripening
Cheese may be the most popular fermented milk product. Cheese is produced
throughout the world in wide-ranging flavors, textures, and forms.
Cheese consists of proteins and fat from milk, usually the milk of cows, buffalo,
goat, or sheep.
14. SKIM MILK:-
fat content reduced to 0.5-2 % by centrifugation.
Extensive use in bakery & confectionery.
Also used for low calorie diets & children who need high
protein.
EVAPORATED MILK:-
50-60% water evaporated
clarified raw milk is concentrated in vaccum pan at 74-77°C ,
fortified withVit D, sterilised in cans at 118°C for 15 minutes & cooled.
As per PFA condensed milk should contain 26% milk solids of which 8% is fat.
Evaporated milk
15. SWEETENED CONDENSED MILK:-
not sterile, made from pasteurized milk concentrated &
with 65% sucrose.
Contains 9% fat out of 31% milk solids.
DRY MILK:-
made with whole milk or skimmed milk dehydrated to about 97%
spray drying & vaccum drying.
Good shelf life.
Highly hygroscopic & can be reconstituted to fluid milk by adding
water.
17. TONED MILK:-
7% fat; mix of reconstituted from skim milk powder with buffalo
milk.
Fat content >5% & SNF 8.5%.
DOUBLE TONED MILK:-
admixture of cow’s or buffalo’s milk or both with fresh skimmed
or by admixture with skim milk reconstituted from skim milk
Should be pasteurized & show negative phosphatase test.
Fat content <1.5% & SNF >9%.
RECOMBINED MILK:-
homogenized product prepared from milk fat, MSNF & water.
Pasteurized
fat content <3% & SNF 8.5%.
18. FILLED MILK:-
homogenized product prepared from refined vegetable oil, MSNF &
Fat content > 3% & SNF 8.5%.
MALTED MILK:-
Malted Milk is a powdered gruel made from a mixture of malted barley,
wheat flour, and whole milk, which is evaporated until it forms a powder.
Malt powder comes in two forms:-
• Diastatic malt contains enzymes that break down starch into sugar;
this is the form that is added to bread dough to help the dough rise
create a good crust.
• Nondiastatic malt has no active enzymes and is used primarily for
flavor, mostly in beverages. It sometimes contains sugar, coloring
agents, and other additives.
19. LACTOSE FREE MILK:-
A lactose-free milk is available for people who are lactose-
intolerant.
This modified milk is made by filtering regular milk to remove
half the lactose.
The enzyme lactase is then added to the milk to break down
remaining lactose into simpler forms which the body can
FLAVORED MILK:-
Different types of flavored milk are available in market.
Some artificial flavoring agents are added in the milk
These can be chocolate , strawberry ,vanilla , butterscotch , elaichi
almonds , coffee , etc.
Nowadays turmeric added milk(haldi dudh) is also available.
20. UHT PROCESSED - MILK:-
Ultra-high Temperature processed milk.
packed & aseptically sealed in pre-sterilized containers under
aseptic conditions.
can be stored Unrefrigerated for at least 3 months.
Cooked flavor due to denaturation of β-lactoglobulin.
STANDARDISED MILK:-
Mix of buffalo & skim milk.
fat is maintained 4.5% and SNF 8.5%.
KHOA:-
semi-solid obtained from milk by evaporating in open pans
continuous stirring in circular motion.
Yield is about 20% of weight of milk used.
21. RABRI:-
concentrated sweetened product comprising several layers of clotted
cream.
Sugar is added to milk & reduced to 1/3 of its original volume.
ICE-CREAMS:-
frozen dairy product consisting of whole milk, skim milk, cream, butter,
condensed milk products or dried milk products.
MF & MSNF constitute 60% of TS giving it a rich flavour, improved body
texture.
Also contains sugar, stabilizer, emulsifier, flavoring material, water & air.
Sugar provides sweetness, smoothness & lowers the freezing point.
Stabilizer prevent formation of ice crystals. Forms gel with water &
improve body & texture. e.g., gelatin, sea weed, china grass & CMC.
Emulsifier help disperse fat globules throughout the mix & prevent
formation, further help make ice-cream dry & stiff.
22. BENEFITS OF MILK & MILK PRODUCTS:-
Milk has long been a popular beverage, not only for its flavor but because of its unique nutritional
package.
Bone health
• Milk and milk products are providers of calcium, phosphorous, magnesium and protein which
are all essential for healthy bone growth and development.
• Adequate consumption of milk and dairy from early childhood and throughout life can help to
make the bones strong and protect them against diseases like osteoporosis
Teeth
• The amounts of calcium and phosphorous in milk and products are also beneficial for the
development and maintenance of healthy teeth.
Skin
• When we apply lotions, facial creams and makeup to your skin, they absorb said products and
the ingredients within.This means that if you’re using a raw milk moisturizing lotion, all the
vitamins will be soaked into your skin and released in your bloodstream.
23. CONCLUSION
To conclude this milk and its products are
useful for human and can be serves as the
basic requirement for life.