The document provides an overview of biobanking from the perspective of a user. It discusses three examples of biobanking: 1) Using postmortem brain samples from the NIH NeuroBioBank to validate findings related to Sturge-Weber syndrome. 2) Establishing a biobank for Sturge-Weber syndrome. 3) Discovering mosaic mutations in autism samples by analyzing genomic data and then validating findings using samples from existing biobanks. It also outlines several issues, lessons, and principles for biobanking including usefulness, existing biobanks, importance of identifiers, role of data science, use of standards, informed consent considerations, and ongoing needs and opportunities.
Jonathan Pevsner - Biobanking: a user's perspective and overviewDataSciSIG
Jonathan Pevsner from Johns Hopkins University presents user stories of biobanks and their use in real research questions. This talk was sponsored by the NIH Data Science Special Interest Group and part of a webinar panel on June 23, 2017 on Global Biobanking and Access to Specimens.
The Genomics Revolution: The Good, The Bad, and The Ugly (UEOP16 Keynote)Emiliano De Cristofaro
The document discusses the genomics revolution and its implications for privacy. It outlines the good of genetic testing and medicine, the bad of collecting sensitive genomic data that is hard to anonymize, and the ugly challenges of balancing privacy and the greater good. It then reviews the history of genome sequencing and cost reductions. The remainder summarizes privacy issues like re-identification risks, kin privacy, and challenges of data sharing. It also outlines cryptography techniques being explored to enable private genomic computation and testing on encrypted genomes. Open problems remain around long-term data storage and usability of privacy techniques.
This document summarizes research on the use of adeno-associated virus (AAV) as a vector for gene therapy. AAV is a promising delivery method due to its low immunogenicity, ability to target specific cell types, and lack of pathogenicity. The document discusses how AAV is being used experimentally to treat diseases like cystic fibrosis, cancer, and heart disease by delivering therapeutic genes. While challenges remain, AAV vectors appear safer than other methods and have the potential to treat many currently incurable diseases.
This document discusses next generation sequencing to identify bacteria, fungi, and viruses from patient samples. It summarizes discussions and presentations from several ear, nose, and throat doctors and researchers on using DNA sequencing to diagnose biofilm infections. DNA sequencing was able to identify more pathogens than traditional cultures and provided faster results. Identifying biofilms is important as they are resistant to antibiotics and cause chronic infections. The document promotes using a molecular diagnostic test from PathoGenius to identify pathogens in patient samples, which it says provides faster, more comprehensive results than cultures and is covered by Medicare.
Advanced Next Generation DNA sequencing can more accurately diagnose infections by identifying bacteria, fungi, and viruses compared to traditional culture techniques. This allows for better treatment decisions. PathoGenius Laboratory uses Next Generation Sequencing to identify microbes in samples, providing physicians with diagnostic results to inform customized treatment. Previous methods of relying only on culture were found to significantly underrepresent the microbes present. Molecular diagnostic techniques provide more comprehensive information about biofilms and chronic infections compared to traditional approaches.
Romain Banchereau is a computational biologist and translational immunologist focused on analyzing immune cell populations and transcriptional profiles from human disease cohorts. He has expertise in genomics analysis of blood and immune cells from infectious and autoimmune disease patients. Through bioinformatics analysis, he identifies biomarkers for disease diagnosis, prognosis, and response to treatment. He currently works as a research associate applying these skills to study lupus, juvenile arthritis, and complications during pregnancy with SLE.
This HIBB presentation provides background information on bases, amino acids, proteins, nucleotides and DNA. The presentation then explains what bioinformatics is, lists some examples, and demonstrates some tools. It demonstrates tools which compare parts of human and chimp genes, and illustrate drug resistance analysis and HIV subtype analysis. It then discusses some ethical and clinical aspects to bioinformatics.
Jonathan Pevsner - Biobanking: a user's perspective and overviewDataSciSIG
Jonathan Pevsner from Johns Hopkins University presents user stories of biobanks and their use in real research questions. This talk was sponsored by the NIH Data Science Special Interest Group and part of a webinar panel on June 23, 2017 on Global Biobanking and Access to Specimens.
The Genomics Revolution: The Good, The Bad, and The Ugly (UEOP16 Keynote)Emiliano De Cristofaro
The document discusses the genomics revolution and its implications for privacy. It outlines the good of genetic testing and medicine, the bad of collecting sensitive genomic data that is hard to anonymize, and the ugly challenges of balancing privacy and the greater good. It then reviews the history of genome sequencing and cost reductions. The remainder summarizes privacy issues like re-identification risks, kin privacy, and challenges of data sharing. It also outlines cryptography techniques being explored to enable private genomic computation and testing on encrypted genomes. Open problems remain around long-term data storage and usability of privacy techniques.
This document summarizes research on the use of adeno-associated virus (AAV) as a vector for gene therapy. AAV is a promising delivery method due to its low immunogenicity, ability to target specific cell types, and lack of pathogenicity. The document discusses how AAV is being used experimentally to treat diseases like cystic fibrosis, cancer, and heart disease by delivering therapeutic genes. While challenges remain, AAV vectors appear safer than other methods and have the potential to treat many currently incurable diseases.
This document discusses next generation sequencing to identify bacteria, fungi, and viruses from patient samples. It summarizes discussions and presentations from several ear, nose, and throat doctors and researchers on using DNA sequencing to diagnose biofilm infections. DNA sequencing was able to identify more pathogens than traditional cultures and provided faster results. Identifying biofilms is important as they are resistant to antibiotics and cause chronic infections. The document promotes using a molecular diagnostic test from PathoGenius to identify pathogens in patient samples, which it says provides faster, more comprehensive results than cultures and is covered by Medicare.
Advanced Next Generation DNA sequencing can more accurately diagnose infections by identifying bacteria, fungi, and viruses compared to traditional culture techniques. This allows for better treatment decisions. PathoGenius Laboratory uses Next Generation Sequencing to identify microbes in samples, providing physicians with diagnostic results to inform customized treatment. Previous methods of relying only on culture were found to significantly underrepresent the microbes present. Molecular diagnostic techniques provide more comprehensive information about biofilms and chronic infections compared to traditional approaches.
Romain Banchereau is a computational biologist and translational immunologist focused on analyzing immune cell populations and transcriptional profiles from human disease cohorts. He has expertise in genomics analysis of blood and immune cells from infectious and autoimmune disease patients. Through bioinformatics analysis, he identifies biomarkers for disease diagnosis, prognosis, and response to treatment. He currently works as a research associate applying these skills to study lupus, juvenile arthritis, and complications during pregnancy with SLE.
This HIBB presentation provides background information on bases, amino acids, proteins, nucleotides and DNA. The presentation then explains what bioinformatics is, lists some examples, and demonstrates some tools. It demonstrates tools which compare parts of human and chimp genes, and illustrate drug resistance analysis and HIV subtype analysis. It then discusses some ethical and clinical aspects to bioinformatics.
The Foundation of P4 Medicine Keynote Presentation as presented by Leroy Hood, M.D., PhD, at the Ohio State University Personalized Health Care National Conference 2010.
The document summarizes research into the molecular landscape of cutaneous neurofibromas (cNFs) based on analysis of tumor samples from the Children's Tumor Foundation (CTF) Biobank. Whole genome sequencing revealed a broad array of NF1 gene mutations in cNFs. RNA sequencing identified immune cell populations within tumors and signaling pathways. Integrative analysis found immune signatures correlate with mutations in CREBBP and CDC27 genes. The CTF Biobank and open data sharing aims to address research hurdles and facilitate new discoveries that could help develop treatments.
As an interdisciplinary field of science, bioinformatics combines biology, computer science, information engineering, mathematics and statistics to analyze and interpret the biological data.
Alex Michael Ward is a virologist and cell biologist seeking a research position in biotech. He has 14 years of experience studying virus-host interactions and identifying therapeutic targets using genetic, proteomic, and cell-based approaches. He is a highly collaborative scientist who has independently managed a research team and obtained external funding. His work has resulted in numerous publications, presentations, and the development of novel assays.
Dubina Michael biomedical technologies at Skolkovoigorod
1. The document discusses interdisciplinary research at the intersection of physics, biology, and medicine using nanotechnologies to study biological processes at the atomic level and develop new targeted cancer treatments.
2. Examples of research include using nanoelectrodes to study cancer cell phenotypes, organic quantum dots for siRNA delivery, and Raman spectroscopy for gene expression analysis without amplification.
3. The goal is to establish a research center in St. Petersburg to conduct full-cycle nanobiotechnology research across multiple fields to develop new drugs and medical equipment.
Genetic Privacy
A challenge to Medico-Legal Norms
Graeme Laurie
(Cambridge university press)
Presentation by Shushan Harutyunyan
Human rights and Biopolitics, 31 October, 2012 CEU
The study aims to determine how human demographics and environmental factors shape the development of microbial communities in hospitals. Samples will be collected daily from patient rooms, staff, surfaces and air/water sources for a year from a newly opened hospital. The data will help understand how microbial succession occurs and how prior occupants influence colonization by pathogens. Quantitative PCR and sequencing will identify microbes, with analyses predicting community changes from environmental shifts.
Molecular biomarkers can be used for several purposes in infectious disease research and clinical practice. These include detecting pathogens, measuring antibody responses, identifying markers of virulence, resistance, and disease severity, and understanding human immune responses and genetic susceptibility. Challenges include lack of sensitivity, mobile genetic elements, and changes in RNA sequences. Whole genome sequencing allows investigation of microbial phylogeny, evolution, and virulence factors.
Poster presentation at the Rare Disease Symposium at Oregon Health & Science University in Portland, Oregon, 2015.
http://openwetware.org/wiki/OHSU_Rare_Disease_Research_Consortium_Symposium_2015
Robert Pesich_PAVA_Stanford Resume v. 8_22_16Robert Pesich
Robert Pesich has extensive experience managing laboratory operations and research projects. He has overseen the daily activities of 25 researchers at Stanford University and the Palo Alto VA, including managing budgets, equipment, and regulatory compliance. Pesich has specialized skills in tissue sample processing, gene expression analysis, and bioinformatics. He has authored several publications characterizing gene expression profiles in normal and diseased tissues. Currently, Pesich also serves as President of a poetry non-profit organization.
Genetic mapping is used to determine the specific location of genes on chromosomes. Knowing gene locations can help identify genetic diseases and their underlying causes. One document describes how researchers used genetic techniques to identify a gene (PSR1) in algae that increases lipid production without subjecting the algae to stress. This could allow for lipid extraction and energy production while preserving the algae species. A second document discusses a new method developed by the University of Haifa to reduce the number of possible genetic effects to study from millions to thousands. The method was tested successfully in a plant with over 7,000 genes. This type of research can help identify genetic diseases more easily and determine the best treatments.
This document provides an introduction to the field of bioinformatics. It defines bioinformatics as the merging of biology, computer science, and information technology into a single discipline. The document outlines key topics in bioinformatics including what is bioinformatics, why it is needed due to the growth of sequencing data, common data types and analysis problems, careers in bioinformatics, and different sequencing technologies such as Illumina and SOLiD sequencing.
PEGS the essential protein and antibody engineering summit Nicole Proulx
PEGS - The Essential Protein Engineering Summit is the premier event for antibody and protein science research and the biologics industry, with more than 1,800 participants in attendance from over 30 countries. Join us in Boston this May 4-8, 2015 to share insights and best practices with colleagues, connect and form new collaborations during copious networking opportunities, learn from world-renowned thought leaders, discover industry trends and find solutions to current challenges. PEGS is knowledge-sharing at its best.
1) A survey of over 1,000 life scientists revealed that while the human genome sequence has transformed biology and inspired new research directions, it has not yet revolutionized medicine as initially hoped.
2) While sequencing technologies have advanced rapidly, making data more abundant, researchers face challenges in analyzing and understanding the vast amounts of data due to limited computing power, lack of appropriate software, and shortage of qualified bioinformaticians.
3) Most researchers now have more modest expectations of what genomics can deliver in the near future, with many predicting personalized medicine based on genetic information will take 10-20 years to become commonplace.
Stephen Friend Genetic Alliance 25th Anniversary 2011-06-24Sage Base
This document discusses using data-intensive science to build better models of human disease. It argues that advances in data generation, computing power, and open information systems now make it possible to comprehensively monitor disease and molecular traits in populations. This could allow evolving disease models in a shared compute space to develop better understanding of complex biological pathways and their relationship to diseases. The document outlines several initiatives, including the Clinical Trial Comparator Arm Partnership and Sage Bionetworks, that aim to facilitate open sharing of genomic and clinical trial data to generate more powerful models and accelerate progress against human diseases.
Research Ethics Forum: Ethical Challenges in Trials of Human Genome Editing a...SC CTSI at USC and CHLA
In her 60-minute presentation, Professor Charo addressed Ethical Challenges in Trials of Human Genome Editing and Gene Therapy, as gene therapy and genome editing clinical trials involve ethical challenges not always found in other areas of research.
Introducing Bioinformatics
Bioinformatics in the Big Data Era
How to get into Bioinformatics?
How to learn and practice Bioinformatics?
Bioinformatics Careers and Salaries Worldwide
Applications of Bioinformatics
Take-Home Messages
Karyotype studying allows doctors to identify genetic variations that can help with diagnosis. Shorter telomeres were found in adults who had more infections as children, possibly due to increased immune cell division. Cancer cells often have irregular chromosome numbers (aneuploidy), which may dampen the immune response to tumors by affecting gene expression. Understanding a patient's karyotype provides insight into factors that influence processes like aging, immunity, and disease treatment responses to personalize care.
UK Biobank: A Prospective Cohort Epidemiology Studyamirhannan
The document discusses UK Biobank, a large prospective cohort study involving 500,000 UK participants. Extensive health and lifestyle data were collected at baseline along with biological samples. Genetic data from samples is being used in genome-wide association studies and other research to study relationships between genes, lifestyle factors and disease. Researchers worldwide can access anonymized UK Biobank data to study links between risk factors and various health conditions like cancer, heart disease and diabetes.
This document provides an overview of the November 2000 issue of JALA (Journal of Analytical Laboratories Automation). It describes the development of a novel robotic system for the New York Cancer Project biorepository in collaboration with the Medical Automation Research Center. The biorepository receives 50-100 blood samples per day which are processed robotically to extract, quantify, aliquot and store DNA, plasma and RNA to be accessible to investigators. The robotic system aims to provide rapid random access to the hundreds of thousands of DNA samples stored for high-throughput analysis in studies of gene-environment interactions and cancer risk.
The Foundation of P4 Medicine Keynote Presentation as presented by Leroy Hood, M.D., PhD, at the Ohio State University Personalized Health Care National Conference 2010.
The document summarizes research into the molecular landscape of cutaneous neurofibromas (cNFs) based on analysis of tumor samples from the Children's Tumor Foundation (CTF) Biobank. Whole genome sequencing revealed a broad array of NF1 gene mutations in cNFs. RNA sequencing identified immune cell populations within tumors and signaling pathways. Integrative analysis found immune signatures correlate with mutations in CREBBP and CDC27 genes. The CTF Biobank and open data sharing aims to address research hurdles and facilitate new discoveries that could help develop treatments.
As an interdisciplinary field of science, bioinformatics combines biology, computer science, information engineering, mathematics and statistics to analyze and interpret the biological data.
Alex Michael Ward is a virologist and cell biologist seeking a research position in biotech. He has 14 years of experience studying virus-host interactions and identifying therapeutic targets using genetic, proteomic, and cell-based approaches. He is a highly collaborative scientist who has independently managed a research team and obtained external funding. His work has resulted in numerous publications, presentations, and the development of novel assays.
Dubina Michael biomedical technologies at Skolkovoigorod
1. The document discusses interdisciplinary research at the intersection of physics, biology, and medicine using nanotechnologies to study biological processes at the atomic level and develop new targeted cancer treatments.
2. Examples of research include using nanoelectrodes to study cancer cell phenotypes, organic quantum dots for siRNA delivery, and Raman spectroscopy for gene expression analysis without amplification.
3. The goal is to establish a research center in St. Petersburg to conduct full-cycle nanobiotechnology research across multiple fields to develop new drugs and medical equipment.
Genetic Privacy
A challenge to Medico-Legal Norms
Graeme Laurie
(Cambridge university press)
Presentation by Shushan Harutyunyan
Human rights and Biopolitics, 31 October, 2012 CEU
The study aims to determine how human demographics and environmental factors shape the development of microbial communities in hospitals. Samples will be collected daily from patient rooms, staff, surfaces and air/water sources for a year from a newly opened hospital. The data will help understand how microbial succession occurs and how prior occupants influence colonization by pathogens. Quantitative PCR and sequencing will identify microbes, with analyses predicting community changes from environmental shifts.
Molecular biomarkers can be used for several purposes in infectious disease research and clinical practice. These include detecting pathogens, measuring antibody responses, identifying markers of virulence, resistance, and disease severity, and understanding human immune responses and genetic susceptibility. Challenges include lack of sensitivity, mobile genetic elements, and changes in RNA sequences. Whole genome sequencing allows investigation of microbial phylogeny, evolution, and virulence factors.
Poster presentation at the Rare Disease Symposium at Oregon Health & Science University in Portland, Oregon, 2015.
http://openwetware.org/wiki/OHSU_Rare_Disease_Research_Consortium_Symposium_2015
Robert Pesich_PAVA_Stanford Resume v. 8_22_16Robert Pesich
Robert Pesich has extensive experience managing laboratory operations and research projects. He has overseen the daily activities of 25 researchers at Stanford University and the Palo Alto VA, including managing budgets, equipment, and regulatory compliance. Pesich has specialized skills in tissue sample processing, gene expression analysis, and bioinformatics. He has authored several publications characterizing gene expression profiles in normal and diseased tissues. Currently, Pesich also serves as President of a poetry non-profit organization.
Genetic mapping is used to determine the specific location of genes on chromosomes. Knowing gene locations can help identify genetic diseases and their underlying causes. One document describes how researchers used genetic techniques to identify a gene (PSR1) in algae that increases lipid production without subjecting the algae to stress. This could allow for lipid extraction and energy production while preserving the algae species. A second document discusses a new method developed by the University of Haifa to reduce the number of possible genetic effects to study from millions to thousands. The method was tested successfully in a plant with over 7,000 genes. This type of research can help identify genetic diseases more easily and determine the best treatments.
This document provides an introduction to the field of bioinformatics. It defines bioinformatics as the merging of biology, computer science, and information technology into a single discipline. The document outlines key topics in bioinformatics including what is bioinformatics, why it is needed due to the growth of sequencing data, common data types and analysis problems, careers in bioinformatics, and different sequencing technologies such as Illumina and SOLiD sequencing.
PEGS the essential protein and antibody engineering summit Nicole Proulx
PEGS - The Essential Protein Engineering Summit is the premier event for antibody and protein science research and the biologics industry, with more than 1,800 participants in attendance from over 30 countries. Join us in Boston this May 4-8, 2015 to share insights and best practices with colleagues, connect and form new collaborations during copious networking opportunities, learn from world-renowned thought leaders, discover industry trends and find solutions to current challenges. PEGS is knowledge-sharing at its best.
1) A survey of over 1,000 life scientists revealed that while the human genome sequence has transformed biology and inspired new research directions, it has not yet revolutionized medicine as initially hoped.
2) While sequencing technologies have advanced rapidly, making data more abundant, researchers face challenges in analyzing and understanding the vast amounts of data due to limited computing power, lack of appropriate software, and shortage of qualified bioinformaticians.
3) Most researchers now have more modest expectations of what genomics can deliver in the near future, with many predicting personalized medicine based on genetic information will take 10-20 years to become commonplace.
Stephen Friend Genetic Alliance 25th Anniversary 2011-06-24Sage Base
This document discusses using data-intensive science to build better models of human disease. It argues that advances in data generation, computing power, and open information systems now make it possible to comprehensively monitor disease and molecular traits in populations. This could allow evolving disease models in a shared compute space to develop better understanding of complex biological pathways and their relationship to diseases. The document outlines several initiatives, including the Clinical Trial Comparator Arm Partnership and Sage Bionetworks, that aim to facilitate open sharing of genomic and clinical trial data to generate more powerful models and accelerate progress against human diseases.
Research Ethics Forum: Ethical Challenges in Trials of Human Genome Editing a...SC CTSI at USC and CHLA
In her 60-minute presentation, Professor Charo addressed Ethical Challenges in Trials of Human Genome Editing and Gene Therapy, as gene therapy and genome editing clinical trials involve ethical challenges not always found in other areas of research.
Introducing Bioinformatics
Bioinformatics in the Big Data Era
How to get into Bioinformatics?
How to learn and practice Bioinformatics?
Bioinformatics Careers and Salaries Worldwide
Applications of Bioinformatics
Take-Home Messages
Karyotype studying allows doctors to identify genetic variations that can help with diagnosis. Shorter telomeres were found in adults who had more infections as children, possibly due to increased immune cell division. Cancer cells often have irregular chromosome numbers (aneuploidy), which may dampen the immune response to tumors by affecting gene expression. Understanding a patient's karyotype provides insight into factors that influence processes like aging, immunity, and disease treatment responses to personalize care.
UK Biobank: A Prospective Cohort Epidemiology Studyamirhannan
The document discusses UK Biobank, a large prospective cohort study involving 500,000 UK participants. Extensive health and lifestyle data were collected at baseline along with biological samples. Genetic data from samples is being used in genome-wide association studies and other research to study relationships between genes, lifestyle factors and disease. Researchers worldwide can access anonymized UK Biobank data to study links between risk factors and various health conditions like cancer, heart disease and diabetes.
This document provides an overview of the November 2000 issue of JALA (Journal of Analytical Laboratories Automation). It describes the development of a novel robotic system for the New York Cancer Project biorepository in collaboration with the Medical Automation Research Center. The biorepository receives 50-100 blood samples per day which are processed robotically to extract, quantify, aliquot and store DNA, plasma and RNA to be accessible to investigators. The robotic system aims to provide rapid random access to the hundreds of thousands of DNA samples stored for high-throughput analysis in studies of gene-environment interactions and cancer risk.
Discovering the 100 Trillion Bacteria Living Within Each of UsLarry Smarr
This document provides a summary of a lecture on the human microbiome given by Dr. Larry Smarr. Some key points:
- The human microbiome refers to the trillions of bacteria that live within the human body. Each person contains 100 trillion bacteria, outnumbering human cells.
- Research into the microbiome is a rapidly growing field that provides insights into health and disease. The microbiome plays a role in processes like drug metabolism and immunity.
- The microbiome is established early in life and influenced by factors like birth method and antibiotic use in the first years. This early development can impact future health.
- Microbiome composition and function can change with health status, diet, medications and other
Discovering the 100 Trillion Bacteria Living Within Each of UsLarry Smarr
This document provides a summary of a lecture on the human microbiome given by Dr. Larry Smarr. Some key points:
- The human microbiome refers to the trillions of bacteria that live within the human body. Each person contains 100 trillion bacteria, outnumbering human cells.
- Research into the microbiome is a rapidly growing field that provides insights into health and disease. The microbiome plays a role in processes like drug metabolism and immunity.
- The microbiome is established early in life and influenced by factors like birth method and antibiotic use in the first years. This early development can impact future health.
- Microbiome imbalances are linked to diseases like inflammatory bowel disease. New treatments are
The document discusses the intersection of precision medicine, biomarkers, and healthcare policy. It describes how biomarkers and -omics data can be used for precision medicine to improve diagnostic accuracy, deliver targeted therapies, and stratify patient populations. However, clinical validation of biomarkers now requires large datasets and years of studies due to regulatory and payer requirements. This has reduced incentives for diagnostic innovation. The document also discusses challenges around clinical interpretation of complex multi-omic tests, evolving medical training and workflows, and disconnects between patent and reimbursement policies.
Bio banking involves collecting and storing biological samples like body fluids and tissues for use in research to improve health and disease. Other data like height, weight, and health history are also recorded. Samples are stored long-term in bio banks, which must carefully protect privacy, access, and sample integrity. Bio banks are classified by purpose and design, such as disease-oriented banks near hospitals or population-based banks. Bio banking enables analysis of rare diseases and high quality tissue collection for research markers and potential therapies. Stem cell banking of umbilical cord blood is also discussed as a rich source of stem cells for transplants and therapeutics. Various ethical, legal and governance issues regarding informed consent, data privacy, and diversity in
Bioinformatics is an interdisciplinary field involving biology, computer science, mathematics and statistics. It addresses large-scale biological problems from a computational perspective. Common problems include modeling biological processes at the molecular level and making inferences from collected data. A bioinformatics solution typically involves collecting statistics from biological data, building a computational model, solving a computational problem, and testing the algorithm. Bioinformatics plays a role in areas like structural genomics, functional genomics and nutritional genomics. It is used for applications such as transcriptome analysis, drug discovery, cheminformatics analysis, and more. It is an important tool in fields like molecular medicine, gene therapy, microbial genome applications, antibiotic resistance, and evolutionary studies. Biological databases are important for organizing
Bioinformatics is a hybrid science that links biological data with techniques for information storage, distribution, and analysis to support multiple areas of scientific research, including biomedicine.
Building an efficient infrastructure, standards and data flow for metabolomicsChristoph Steinbeck
Christoph Steinbeck from the European Bioinformatics Institute discusses developing efficient infrastructure, standards, and data flow for metabolomics. Metabolomics measures small molecule metabolites in organisms and generates large amounts of data. The MetaboLights database was created to share metabolomics data openly. It is growing rapidly with a doubling time of 3 months for metabolomics data. Efforts are underway to build standardized reference data through projects like COSMOS and MetabolomeXchange. While genomes of thousands of species have been sequenced, far fewer complete metabolomes exist. The talk advocates for focused efforts to map metabolites and build quantitative models of well-studied model organisms' metabolomes.
This document provides an overview of biotechnology and related topics. It defines biotechnology as the integration of science and engineering to life processes to solve problems or manufacture products. It discusses core biotechnologies like monoclonal antibodies, biosensors, cell culture, and recombinant DNA. It explains how these biotechnologies are used in areas like healthcare, pharmaceuticals, and environmental remediation. It also summarizes the science of cells, DNA, genes, and proteins as the foundations of modern biotechnology.
Lecture given for the Data Mining course at Uppsala university in October 2013. The presentation talks about data analysis in the context of genomics, next-generation sequencing, metagenomics etc.
Human Genome Sequencing and health Biotechnology.ppthkk03012587
The document discusses the impact of human genome sequencing in health biotechnology. It describes how DNA sequencing allows comparisons between individuals and species, and how the Human Genome Project mapped the entire human genome. The key lessons learned are that the human genome contains around 20,000-25,000 genes, only 3% of DNA codes for proteins, and knowledge of gene expression helps understand human biology and disease. The document also discusses ethical issues raised by genomic data and applications of biotechnology like gene therapy, stem cells, designer drugs, DNA in forensics, agriculture, and environmental bioremediation.
Gene Wiki and Mark2Cure update for BD2KBenjamin Good
The document discusses using crowdsourcing via platforms like Amazon Mechanical Turk and Mark2Cure to extract information from biomedical literature at scale. It summarizes experiments showing non-experts can accurately recognize disease concepts in PubMed abstracts when aggregated. The author proposes expanding this approach to identify genes, drugs, diseases and relationships to build a computable network of biomedical knowledge from the literature. Funding sources and collaborators supporting various related projects are acknowledged at the end.
Using Supercomputers and Gene Sequencers to Discover Your Inner MicrobiomeLarry Smarr
This keynote talk discusses research using supercomputers and gene sequencing to study the human microbiome. The human microbiome contains 100 trillion microorganisms and their genes outnumber human genes 300 to 1. The speaker has been collecting data from his own body over 7 years to study his microbiome and immune system interactions. Collaborating researchers have sequenced his gut microbiome over time as well as samples from autoimmune disease patients. Supercomputers are needed to analyze the massive amount of sequencing data and reveal details of microbial ecology and genetics in health and disease. Studying the human microbiome will revolutionize medicine in the next decade.
Finding the Patterns in the Big Data From Human Microbiome EcologyLarry Smarr
This document summarizes a talk on analyzing human microbiome data to better understand health and disease. It discusses how sequencing and supercomputing is used to map microbial ecology in hundreds of people. Advanced analytics tools like Ayasdi are helping discover patterns separating healthy from disease states like inflammatory bowel disease. Future goals include applying these techniques to larger datasets and using molecular networks to better understand disease development at the genetic and protein level.
Big Data and the Promise and Pitfalls when Applied to Disease Prevention and ...Philip Bourne
Big data and data science have implications for healthcare and biomedical research. Large amounts of data are being generated but much of it remains unused. Integrating data through common standards could provide new insights into rare diseases. The National Institutes of Health is working to establish data standards and cloud resources to enable data sharing and advance precision medicine through its Precision Medicine Initiative. Data science has the potential to improve disease prevention and health promotion by identifying patterns in large, diverse datasets.
Emerging collaboration models for academic medical centers _ our place in the...Rick Silva
- The document discusses emerging collaboration models between academic medical centers and other organizations in the genomics and precision medicine field, as genomic sequencing capabilities advance and more clinical cases are needed to power artificial intelligence platforms. It explores new partnership approaches around data sharing, patient engagement, infrastructure needs, and how academic medical centers can position themselves in this evolving ecosystem.
This document discusses harnessing the power of teams and networks to build better models of disease in real time. It notes that new technologies now allow the generation of massive amounts of human omics data and emerging network modeling approaches for diseases. Cloud computing infrastructure allows a generative open approach to biomedical problem solving. A nascent movement aims to give patients more control over their sensitive health information to facilitate sharing. Open social media also allows experts and citizens to collaborate to solve biomedical problems. The overall opportunity is to conduct more open, collaborative biomedical research involving diverse teams.
Similar to NIH Data Science Special Interest Group (20)
Travel vaccination in Manchester offers comprehensive immunization services for individuals planning international trips. Expert healthcare providers administer vaccines tailored to your destination, ensuring you stay protected against various diseases. Conveniently located clinics and flexible appointment options make it easy to get the necessary shots before your journey. Stay healthy and travel with confidence by getting vaccinated in Manchester. Visit us: www.nxhealthcare.co.uk
share - Lions, tigers, AI and health misinformation, oh my!.pptxTina Purnat
• Pitfalls and pivots needed to use AI effectively in public health
• Evidence-based strategies to address health misinformation effectively
• Building trust with communities online and offline
• Equipping health professionals to address questions, concerns and health misinformation
• Assessing risk and mitigating harm from adverse health narratives in communities, health workforce and health system
Promoting Wellbeing - Applied Social Psychology - Psychology SuperNotesPsychoTech Services
A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!
Histololgy of Female Reproductive System.pptxAyeshaZaid1
Dive into an in-depth exploration of the histological structure of female reproductive system with this comprehensive lecture. Presented by Dr. Ayesha Irfan, Assistant Professor of Anatomy, this presentation covers the Gross anatomy and functional histology of the female reproductive organs. Ideal for students, educators, and anyone interested in medical science, this lecture provides clear explanations, detailed diagrams, and valuable insights into female reproductive system. Enhance your knowledge and understanding of this essential aspect of human biology.
These lecture slides, by Dr Sidra Arshad, offer a simplified look into the mechanisms involved in the regulation of respiration:
Learning objectives:
1. Describe the organisation of respiratory center
2. Describe the nervous control of inspiration and respiratory rhythm
3. Describe the functions of the dorsal and respiratory groups of neurons
4. Describe the influences of the Pneumotaxic and Apneustic centers
5. Explain the role of Hering-Breur inflation reflex in regulation of inspiration
6. Explain the role of central chemoreceptors in regulation of respiration
7. Explain the role of peripheral chemoreceptors in regulation of respiration
8. Explain the regulation of respiration during exercise
9. Integrate the respiratory regulatory mechanisms
10. Describe the Cheyne-Stokes breathing
Study Resources:
1. Chapter 42, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 36, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 13, Human Physiology by Lauralee Sherwood, 9th edition
- Video recording of this lecture in English language: https://youtu.be/Pt1nA32sdHQ
- Video recording of this lecture in Arabic language: https://youtu.be/uFdc9F0rlP0
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
One health condition that is becoming more common day by day is diabetes.
According to research conducted by the National Family Health Survey of India, diabetic cases show a projection which might increase to 10.4% by 2030.
Hiranandani Hospital in Powai, Mumbai, is a premier healthcare institution that has been serving the community with exceptional medical care since its establishment. As a part of the renowned Hiranandani Group, the hospital is committed to delivering world-class healthcare services across a wide range of specialties, including kidney transplantation. With its state-of-the-art facilities, advanced medical technology, and a team of highly skilled healthcare professionals, Hiranandani Hospital has earned a reputation as a trusted name in the healthcare industry. The hospital's patient-centric approach, coupled with its focus on innovation and excellence, ensures that patients receive the highest standard of care in a compassionate and supportive environment.
8 Surprising Reasons To Meditate 40 Minutes A Day That Can Change Your Life.pptxHolistified Wellness
We’re talking about Vedic Meditation, a form of meditation that has been around for at least 5,000 years. Back then, the people who lived in the Indus Valley, now known as India and Pakistan, practised meditation as a fundamental part of daily life. This knowledge that has given us yoga and Ayurveda, was known as Veda, hence the name Vedic. And though there are some written records, the practice has been passed down verbally from generation to generation.
1. Biobanking: a user’s perspective
and an overview
Jonathan Pevsner, Ph.D.
Professor, Dept. of Neurology
Kennedy Krieger Institute and Johns Hopkins Medicine
Chief Scientific Officer, Sturge-Weber Foundation
pevsner@kennedykrieger.org
Data Science Forum: NIH Data Science SIG
Global Perspective on Biobanking and Access to Samples
June 23, 2017
3. Outline
From genotype to phenotype: a framework
Three biobanking examples
Postmortem brains from the NIH NeuroBioBank
Establishing a rare disease biobank
From a large genomics dataset to biobank samples
Issues, lessons and principles
1. Usefulness
2. Existing biobanks
3. GUIDs: the importance of labels
4. Data science is integral to biobanking
5. Standards
6. Informed consent
7. Needs and opportunities
4. The relationship between genotype and phenotype
represents one of the most fundamental and challenging
problems in biomedical science.
Fundamental framework: genotype to phenotype
Genotype Phenotype
5. Fundamental framework: genotype to phenotype
Genotype Phenotype
DNA individual populationorgancellprotein
We can provide a framework for this problem.
6. Fundamental framework: genotype to phenotype
Genotype Phenotype
DNA individual populationorgancellprotein
We can provide a framework for this problem.
RNA pathways circuits
7. Fundamental framework: genotype to phenotype
Genotype Phenotype
DNA individual populationorgancellprotein
gene1
gene20,000
gene2
…
8. Fundamental framework: genotype to phenotype
Genotype Phenotype
DNA individual populationorgancellprotein
gene1
gene20,000
gene2
ABCD1
severe childhood disease (ALD)
mild adult onset disease (AMN)
apparently normal
One gene mutation can have different phenotypic
consequences: the same ABCD1 mutation may result in severe
childhood-onset adrenoleukodystrophy (ALD), milder adult-
onset adrenomyeloneuropathy (AMN), or no symptoms.
9. Fundamental framework: genotype to phenotype
Genotype Phenotype
DNA individual populationorgancellprotein
gene1
gene20,000
gene2
GNAQ
melanocytes: uveal melanoma
endothelial cells: Sturge-Weber
blood: apparently normal
One gene mutation can have different consequences:
when and where mutations occur is crucial.
10. Fundamental framework: genotype to phenotype
Genotype Phenotype
DNA individual populationorgancellprotein
gene1
gene20,000
gene2
…
one disease phenotype,
multiple genetic contributors
For almost all diseases (including common diseases such
as autism or bipolar disorder) we search for multiple
genetic variants that confer risk for a phenotype
11. Outline
From genotype to phenotype: a framework
Three biobanking examples
Postmortem brains from the NIH NeuroBioBank
Establishing a rare disease biobank
From a large genomics dataset to biobank samples
Issues, lessons and principles
1. Usefulness
2. Existing biobanks
3. GUIDs: the importance of labels
4. Data science is integral to biobanking
5. Standards
6. Informed consent
7. Needs and opportunities
12. A port-wine birthmark affects about 1:300 people.
It varies in size and location.
Sturge-Weber syndrome affects < 1:20,000 people.
It affects a subset of individuals with a facial PW birthmark.
A user’s perspective on biobanking: three examples.
(1) Sturge-Weber syndrome and a brain bank
13. A user’s perspective on biobanking: three examples.
(1) Sturge-Weber syndrome and a brain bank
DNA from
blood
(presumed
unaffected)
DNA from port-
wine birthmark
(presumed
affected)
14. A user’s perspective on biobanking: three examples.
(1) Sturge-Weber syndrome and a brain bank
DNA from
blood
(presumed
unaffected)
DNA from port-
wine birthmark
(presumed
affected)
sequence the
genome
sequence the
genome
compare
We identified a mosaic mutation in GNAQ as causing Sturge-
Weber syndrome and port-wine birthmarks (NEJM, PMID
23656586).We analyzed samples from 3 individual patients.
15. A user’s perspective on biobanking: three examples.
(1) Sturge-Weber syndrome and a brain bank
Genotype Phenotype
DNA individual populationorgancellprotein
gene1
gene20,000
gene2
GNAQ
After finding the GNAQ mutation we turned to the NIH
NeuroBioBank at the University of Maryland.We obtained
97 samples to validate our findings.The availability of these
samples from a biobank was crucial!
16. A user’s perspective on biobanking: three examples.
(2) Establishing a Sturge-Weber syndrome biobank
I am Chief Scientific Officer of the Sturge-Weber
Foundation.We need to create (or join) a biobank.
• Patients and families tell me “I want to donate my brain
and body to science. Can you help?”What’s the plan;
and are there informed consent issues?
• Scientists have discovered that the GNAQ mutation
occurs primarily in endothelial cells, and cell lines
have been established from brain biopsies. How can
researchers share and access these cell lines?
• Are there standards that we should follow in describing
the genotype and phenotype of Sturge-Weber
syndrome samples and patients?
• Have these problems been addressed by those studying
related diseases?
17. A user’s perspective on biobanking: three examples.
(2) Establishing a Sturge-Weber syndrome biobank
Genotype Phenotype
DNA individual populationorgancellprotein
gene1
gene20,000
gene2
GNAQ
It’s important to link clinical data (e.g. from a patient
registry) with data generated from biospecimens!
18. A user’s perspective on biobanking: three examples.
(2) Establishing a Sturge-Weber syndrome biobank
Genotype Phenotype
DNA individual populationorgancellprotein
gene1
gene20,000
gene2
GNAQ
What information do we
need to capture about cell
lines, brain, and skin samples?
19. A user’s perspective on biobanking: three examples.
(2) Establishing a Sturge-Weber syndrome biobank
Genotype Phenotype
DNA individual populationorgancellprotein
gene1
gene20,000
gene2
GNAQ
What information do we
need to capture about cell
lines, brain, and skin samples?
How do we relate genomic DNA
sequence findings, RNA-seq,
proteomics to the samples?
20. A user’s perspective on biobanking: three examples.
(2) Establishing a Sturge-Weber syndrome biobank
Genotype Phenotype
DNA individual populationorgancellprotein
gene1
gene20,000
gene2
GNAQ
What information do we
need to capture about cell
lines, brain, and skin samples?
What information do we need
to capture about the
phenotypes as we collect
samples at diverse sites?
How do we relate genomic DNA
sequence findings, RNA-seq,
proteomics to the samples?
21. A user’s perspective on biobanking: three examples.
(3) Discovering mosaic mutations in 9000 autism samples
We asked whether mosaic mutations occur in autism. By
applying to NIH we obtained previously generated whole
exome sequence data on 9000 individuals via the Simons
Simplex Collection (SSC).We discovered mosaic variation is
enriched in children with autism spectrum disorder.
To validate our findings, we applied to the Simons
Foundation and received approval to obtain DNA from a
Rutgers repository (http://www.rucdr.org/).We purchased
300 DNA samples and successfully validated our findings.
See PMID 27632392:
22. A user’s perspective on biobanking: three examples.
(3) Discovering mosaic mutations in 9000 autism samples
Genotype Phenotype
DNA individual populationorgancellprotein
gene1
gene20,000
gene2
…
A user starts with
genomics data…
23. A user’s perspective on biobanking: three examples.
(3) Discovering mosaic mutations in 9000 autism samples
Genotype Phenotype
DNA individual populationorgancellprotein
gene1
gene20,000
gene2
…
…then purchases cell lines
or DNA or brain chunks
for further studies…
A user starts with
genomics data…
24. A user’s perspective on biobanking: three examples.
(3) Discovering mosaic mutations in 9000 autism samples
Genotype Phenotype
DNA individual populationorgancellprotein
gene1
gene20,000
gene2
…
…then purchases cell lines
or DNA or brain chunks
for further studies…
Obtaining clinical phenotypes
from the biobank is essential.
A user starts with
genomics data…
25. Outline
From genotype to phenotype: a framework
Three biobanking examples
Postmortem brains from the NIH NeuroBioBank
Establishing a rare disease biobank
From a large genomics dataset to biobank samples
Issues, lessons and principles
1. Usefulness
2. Existing biobanks
3. GUIDs: the importance of labels
4. Data science is integral to biobanking
5. Standards
6. Informed consent
7. Needs and opportunities
26. (1) Usefulness
• Diseases are considered rare when affecting 200,000
or fewer people (U.S. definition) or fewer than
1:2,000 people (European definition).
• There are ~6,800 rare diseases.
• Biobanks offer crucial resources to help solve the
causes of rare diseases—and to study diagnosis,
prevention, and treatment.
• Biobanks offer a range of cell, solid tissue types (e.g.
brain, heart, fibroblasts, lymphoblastoid cell
lines) and bodily fluids.
• Biobanks offer biospecimens from individuals,
pedigrees, and/or populations.
• Samples from biobanks are complemented by
phenotypic and genotypic data.
27. List of panelists
Jonathan Pevsner, Professor, at the Dept. of Neurology, Kennedy Krieger
Institute. Presentation title: Biobanking user’s perspective and an overview
Dept. of Psychiatry and Behavioral Sciences, Johns Hopkins Medicine
David van Enckevort, Project Manager BBMRI & RD-Connect,Department of
Genetics, University Medical Center Groningen (UMCG). Presentation title: “FAIR
(Findable, Accessible, Interoperable and Reusable) data and sample access “
Manuel Posada de la Paz. Director, Research Institute for Rare Diseases
(Instituto de Investigación en Enfermedades Raras), a member of the EuroBioBank.
Presentation title: Rare diseases biological samples: small collections and research.
Kerry Wiles, Program Director- VUMC Tissue Repository, CHTN (Cooperative
Human Tissue Network) Western Coordinator. Presentation title: An academic
prospective procurement repository: From Donor to Bench
Jim Vaught Editor-in-Chief, Biopreservation Journal, past President of the
International Society for Biological and Environmental Repositories (ISBER), on the
board of directors for ISBER and NDRI (National Disease Research Interchange),
Presentation title: "NIH and ISBER perspectives on specimen locators"
Daniel Catchpoole Director of Kids Research Institute, The Children's Hospital at
Westmead (Australia). Presentation title: The Australian experience, issues and
solution
28. (2) Examples of existing biobanks and biobank initiatives
Coriell Biorepository
The NIGMS collection has >11,000 cell lines and
~6,000 DNA samples.
https://catalog.coriell.org/
NIH NeuroBioBank
6 sites.The University of Maryland Brain &Tissue Bank
has distributed 35,000 tissue samples to >900
researchers.
https://neurobiobank.nih.gov/
Cooperative Human Tissue Network (CHTN)
Supported by the National Cancer Institute
https://www.chtn.org/
29. EuroBioBank
130,000 samples available; 13,000 collected and >7,000
samples distributed per year.
http://www.eurobiobank.org/
RD-Connect
"An integrated platform connecting databases,
registries, biobanks and clinical bioinformatics for rare
disease research.”
http://rd-connect.eu/
Research Institute for Rare Diseases
(Instituto de Investigación en Enfermedades Raras), a
member of the EuroBioBank.
http://www.eurobiobank.org/en/partners/description/
isciii.htm
(2) Examples of existing biobanks and biobank initiatives
30. BBMRI-ERIC
Biobanking and biomolecular resources research
infrastructure-European Research Infrastructure
Consortium.
http://www.bbmri-eric.eu/BBMRI-ERIC/common-service-it/
Kids Research Institute,The Children's Hospital at
Westmead (Australia)
http://www.kidsresearch.org.au/our-facilities/bio-banks
National Disease Research Interchange (NDRI)
The mission of NDRI is to provide human biospecimens to
advance biomedical/bioscience research and development
worldwide.”
http://ndriresource.org/
(2) Examples of existing biobanks and biobank initiatives
31. All of Us
“The All of Us Research Program seeks to extend
precision medicine to all diseases by building a national
research cohort of one million or more U.S. participants.”
It includes a biobank.
https://allofus.nih.gov/about/program-components
NIMH Repository and Genomics Resource (NIMH-RGR)
“…plays a key role in facilitating psychiatric genetic
research by providing a collection of over 150,000 well
characterized, high quality patient and control samples
from a wide-range of mental disorders.”
https://www.nimhgenetics.org/
(2) Examples of existing biobanks and biobank initiatives
32. (3) GUIDs: the importance of labels
“Accession numbers” are alpha-numeric characters that
provide links to various kinds of data or records. For
example, NP_620258.1 is an accession number
corresponding to a protein sequence.
A GUID is a Global Unique Identifier that corresponds to
a study participant.The GUID facilitates tracking patient’s
data across studies and location and over time in a
deidentified manner.
Example 1: a participant was recruited twice (years apart)
to a single study.
Example 2: a trio was recruited into two separate autism
genome sequencing studies (one study excluded severe
phenotypes, one excluded mild phenotypes).The proband’s
phenotype had become severe over time.
33. (4) Data science is integral to biobanking
Biobanking requires a series of tasks such as obtaining
biospecimens and associated metadata (e.g. phenotypic
data, cause of death, postmortem interval, cell culture
conditions, imaging data, genomics data).
Goals include effective communication, standardization
(e.g. of protocols), and an electronic portal to a
repository.
All this requires data science.
34. Biobanks must integrate diverse data types
Genotype Phenotype
DNA individual populationorgancellprotein
gene1
gene20,000
gene2
…
Sequence data:
Genomic DNA
(dbGaP), RNAseq
Proteomics data,
metabolomics
imaging data
phenotypic test
data (e.g.
neuropsychology)
cell culture,
biochemical,
iPSC data
epidemiology
35. (5) Standards
Biobanks implement Data Dictionaries to manage data
elements (and data structures) in a uniform manner.The
use of Common Data Elements is crucial.
NIH Common Data Elements (CDE) Repository
“designed to provide access to structured human and
machine-readable definitions of data elements.”
https://cde.nlm.nih.gov/home
37. (6) Informed consent
Research studies (in contrast to clinical tests) are under
Institutional Review Board (IRB) jurisdiction.A
researcher must have a research protocol approved,
and one or more consent forms.
Biobanks provide biospecimens that are sometimes in
the realm of human subjects research.Appropriate
consent forms must be administered for biospecimens
to be deposited in a biobank.
An emerging issue is obtaining appropriate consent for
DNA to be sequenced from biospecimens. Because of
the nature of contemporary sequencing samples are no
longer inherently deidentifiable.
38. (7) Needs and opportunities
We need resources and efforts such as the following:
• coordination of biobanking efforts across diverse
initiatives.
• awareness and adoption of community standards for
biobanking.
• flexibility to adapt to changing technologies (e.g.
sequencing technologies).
• Integrated platforms and bioinformatics tools