This document provides a summary of a report by Yole Développement on next-generation DNA sequencing and DNA synthesis technologies. The report provides an overview of the market and technology landscapes, including descriptions of established and emerging sequencing technologies. It also analyzes the supply chain and provides market data, forecasts, and analysis. The report finds that the number of sequencing instruments is expected to more than double by 2024, driving growth in the number of sequencing consumables from 1.28 million units in 2018 to 4.19 million units in 2024. This rapid adoption represents significant opportunities for companies along the semiconductor supply chain to provide enabling technologies for sequencing consumables.
This document provides background information on genetic sequencing techniques. It begins with a brief history of Sanger sequencing and its role in decoding genetic sequences. It then discusses how DNA can be separated by size using gel electrophoresis, noting that polyacrylamide gels allow for greater resolution than agarose gels. The document goes on to explain how Sanger sequencing works and some improvements that were made over time. It also introduces next-generation sequencing techniques and discusses their advantages over Sanger sequencing in providing massively parallel sequencing at lower cost.
Introduction to Next-Generation Sequencing (NGS) TechnologyQIAGEN
The continuous evolution of NGS technology has led to an enormous diversification in NGS applications and dramatically decreased the costs to sequence a complete human genome.
In this presentation, we will discuss the following major topics:
• Basic overview of NGS sequencing technologies
• Next-generation sequencing workflow
• Spectrum of NGS applications
• QIAGEN universal NGS solutions
This document provides an overview of next generation sequencing (NGS) technologies. It discusses the history and evolution of DNA sequencing, from early manual methods developed by Sanger to modern high-throughput NGS approaches. Key NGS methods described include Illumina sequencing by synthesis, Ion Torrent semiconductor sequencing, 454 pyrosequencing, and SOLiD ligation sequencing. Compared to Sanger, NGS allows massively parallel sequencing of many samples at lower cost and higher throughput. While NGS has advanced biological research, each method still has advantages and limitations related to read length, accuracy, and cost.
The document describes the process and components of emulsion PCR. Key points include:
- Emulsion PCR is used to amplify DNA in microreactors formed from water-in-oil emulsions, allowing individual DNA fragments to be amplified clonally.
- The emulsion PCR mixture contains primers, DNA polymerase, nucleotides, template DNA, and is emulsified in an oil phase containing surfactants to form water-in-oil droplets.
- The emulsion undergoes PCR cycling to amplify the DNA fragments clonally within individual droplets. The emulsion is then broken and the amplified DNA fragments can be analyzed by gel electrophoresis or used for downstream applications like sequencing.
This document discusses the history and evolution of DNA sequencing technologies. It begins with early manual sequencing methods developed in the 1970s by Sanger and others. Automated Sanger sequencing and the sequencing of larger genomes followed in the 1980s-1990s. Next generation sequencing (NGS) methods were developed starting in 1996 and became commercially available in 2005, enabling massively parallel sequencing. NGS platforms such as 454, Illumina, and SOLiD are discussed. Third generation real-time sequencing methods such as PacBio and nanopore sequencing are also introduced, providing longer read lengths. The document compares key parameters of different sequencing methods such as read length, accuracy, throughput, cost and advantages/disadvantages.
This document provides an overview of DNA microarrays. It begins with a brief introduction defining DNA microarrays and their use in analyzing gene expression. Next, it discusses the history and basic aspects of microarrays, including how oligonucleotides are coupled to a surface, sample preparation and hybridization, and scanning and data analysis. Applications of microarrays like gene expression analysis and limitations are also outlined. The document concludes with references used to compile the information presented.
This document provides background information on genetic sequencing techniques. It begins with a brief history of Sanger sequencing and its role in decoding genetic sequences. It then discusses how DNA can be separated by size using gel electrophoresis, noting that polyacrylamide gels allow for greater resolution than agarose gels. The document goes on to explain how Sanger sequencing works and some improvements that were made over time. It also introduces next-generation sequencing techniques and discusses their advantages over Sanger sequencing in providing massively parallel sequencing at lower cost.
Introduction to Next-Generation Sequencing (NGS) TechnologyQIAGEN
The continuous evolution of NGS technology has led to an enormous diversification in NGS applications and dramatically decreased the costs to sequence a complete human genome.
In this presentation, we will discuss the following major topics:
• Basic overview of NGS sequencing technologies
• Next-generation sequencing workflow
• Spectrum of NGS applications
• QIAGEN universal NGS solutions
This document provides an overview of next generation sequencing (NGS) technologies. It discusses the history and evolution of DNA sequencing, from early manual methods developed by Sanger to modern high-throughput NGS approaches. Key NGS methods described include Illumina sequencing by synthesis, Ion Torrent semiconductor sequencing, 454 pyrosequencing, and SOLiD ligation sequencing. Compared to Sanger, NGS allows massively parallel sequencing of many samples at lower cost and higher throughput. While NGS has advanced biological research, each method still has advantages and limitations related to read length, accuracy, and cost.
The document describes the process and components of emulsion PCR. Key points include:
- Emulsion PCR is used to amplify DNA in microreactors formed from water-in-oil emulsions, allowing individual DNA fragments to be amplified clonally.
- The emulsion PCR mixture contains primers, DNA polymerase, nucleotides, template DNA, and is emulsified in an oil phase containing surfactants to form water-in-oil droplets.
- The emulsion undergoes PCR cycling to amplify the DNA fragments clonally within individual droplets. The emulsion is then broken and the amplified DNA fragments can be analyzed by gel electrophoresis or used for downstream applications like sequencing.
This document discusses the history and evolution of DNA sequencing technologies. It begins with early manual sequencing methods developed in the 1970s by Sanger and others. Automated Sanger sequencing and the sequencing of larger genomes followed in the 1980s-1990s. Next generation sequencing (NGS) methods were developed starting in 1996 and became commercially available in 2005, enabling massively parallel sequencing. NGS platforms such as 454, Illumina, and SOLiD are discussed. Third generation real-time sequencing methods such as PacBio and nanopore sequencing are also introduced, providing longer read lengths. The document compares key parameters of different sequencing methods such as read length, accuracy, throughput, cost and advantages/disadvantages.
This document provides an overview of DNA microarrays. It begins with a brief introduction defining DNA microarrays and their use in analyzing gene expression. Next, it discusses the history and basic aspects of microarrays, including how oligonucleotides are coupled to a surface, sample preparation and hybridization, and scanning and data analysis. Applications of microarrays like gene expression analysis and limitations are also outlined. The document concludes with references used to compile the information presented.
Next-generation sequencing (NGS) has various applications in livestock genetics and breeding including:
1. Whole genome sequencing to identify genetic variations within and between species and quantify introgression.
2. RNA sequencing to detect differentially expressed genes between control and infected/challenged animals and identify genes related to disease resistance.
3. Genome-wide association studies using SNPs identified through NGS to map quantitative trait loci and guide marker-assisted selection for improved traits.
The document summarizes the principle and workflow of Illumina next-generation sequencing. It begins with an overview of Illumina and the development of their sequencing technologies. It then describes the wide range of applications of NGS. The core principle is sequencing by synthesis using reversible dye-terminators. The workflow involves library preparation through fragmentation and ligation of adapters, cluster generation by bridge amplification on a flow cell, and sequencing through cycles of reversible terminator incorporation and imaging. Finally, the sequenced reads are aligned and analyzed using Illumina's data analysis software suite.
Next-generation DNA sequencing technologies have significantly impacted genetics research. Three major platforms - Roche/454, Illumina Genome Analyzer, and Applied Biosystems SOLiD - utilize massively parallel sequencing to generate large amounts of sequence data. Roche/454 uses emulsion PCR to amplify DNA fragments on beads and pyrosequencing to determine sequences. Illumina performs bridge amplification on a flow cell to generate DNA clusters then sequences by synthesis. Applied Biosystems SOLiD uses ligation-based sequencing. These new methods have enabled genome-wide studies and applications such as ancient DNA sequencing and metagenomics that were previously difficult or impossible.
This document discusses DNA sequencing methods, both current and developing technologies. It begins by explaining Sanger sequencing and how fluorescent dyes and thermal cycling improved it. High-throughput short and long-read sequencing methods are then outlined, including Illumina, Ion Torrent, Nanopore, and SMRT sequencing. Developing methods like tunneling currents, hybridization, and microscopy techniques are also mentioned. Overall, the document provides a comprehensive overview of the major DNA sequencing techniques used today and those under investigation.
This document discusses the use of organoid cultures in cancer research. It begins with an overview of what organoids are and provides a simple definition of organoids as miniature organs grown in vitro. The document then discusses how three-dimensional culture techniques have enabled the real-time study of mammalian tissues by allowing independent manipulation of genetic and microenvironmental factors. Examples are provided of different cellular inputs used in 3D cultures as well as different culture formats. The roles of 3D cultures in advancing cancer therapeutics through predictive and prognostic testing of preclinical treatments are also summarized.
CRISPR is easily the best gene editing tool to date. For decades, scientists have been looking for a way to to perform precise changes to genetic sequences. In the past several years, researchers were able to exploit the immune systems of bacteria to edit the genome of other living cells. CRISPR is reported to have higher targeting efficiencies when compared to TALENs and Zinc Fingers. It is efficient, easy to use and cheap; making it a scalable genetic engineering tool that is highly desirable in various industry-wide applications.
Synthetic biology is the design and construction of novel artificial biological pathways, organisms, or devices. This document discusses synthetic biology tools and applications in pathway engineering, with a focus on plants. It provides examples of introducing the artemisinin biosynthesis pathway into tobacco using a combinatorial supertransformation approach, and engineering yeast to produce the sesquiterpene α-santalene. While synthetic biology has potential applications in health, environment and energy, it also faces risks that must be addressed including unintentional release of modified organisms and dual-use concerns.
Next generation sequencing techniques have revolutionized DNA sequencing by increasing throughput and decreasing costs compared to previous methods like Sanger sequencing. Some key next generation sequencing methods include 454 sequencing (pyrosequencing), ABI Solid sequencing (sequencing by ligation), Illumina/Solexa sequencing (sequencing by synthesis), and nanopore sequencing. These new techniques allow for faster and cheaper large-scale sequencing and have enabled applications like whole genome sequencing.
Next generation sequencing (NGS) provides a high-throughput and cheaper alternative to DNA sequencing through massively parallel sequencing of millions of DNA fragments simultaneously. NGS can be used for target sequencing to identify disease-causing mutations, RNA sequencing to study entire transcriptomes, and has various applications in cancer research and treatment including identifying mutations that predict responses to immunotherapy. However, NGS also faces challenges like accurately sequencing regions with repeats and detecting fusion genes.
The document discusses the history and evolution of DNA sequencing techniques. It describes first generation Sanger sequencing and how next generation sequencing (NGS) allows for massively parallel sequencing of entire human genomes in a single day. The principles of NGS involve fragmenting DNA, ligating adaptors, sequencing in parallel, and reassembling the results. Common NGS methods include sequencing by synthesis, pyrosequencing, and ion semiconductor sequencing. Applications of NGS include rapidly sequencing whole genomes, detecting rare mutations, studying gene expression, and analyzing the human microbiome.
Single Nucleotide Polymorphism Analysis
Predictive Analytics and Data Science Conference May 27-28
Asst. Prof. Vitara Pungpapong, Ph.D.
Department of Statistics
Faculty of Commerce and Accountancy
Chulalongkorn University
Production and Purification of Virus Like Particle (VLP) based VaccineDr. Priyabrata Pattnaik
This document summarizes a presentation on the production and purification of virus-like particle (VLP) based vaccines. It discusses using hepatitis C VLPs as a model system produced using an insect cell/baculovirus expression platform. Key points covered include:
- Challenges in VLP vaccine production include low yields, stability issues, and difficulties removing baculovirus.
- Hepatitis C VLPs containing E1 and E2 glycoproteins were successfully produced using Sf9 insect cells in a Mobius 3L disposable bioreactor, with comparable results to a glass bioreactor.
- A depth filtration clarification process achieved around 70% DNA clearance while recovering approximately 70
Role of Antisense and RNAi-based Gene Silencing in Crop ImprovementMariya Zaman
This document presents information on RNA interference (RNAi) and its application in crop improvement. It discusses the discovery of antisense RNA and RNAi technology. The mechanisms of antisense technology and RNAi are described. Advantages of RNAi include its ability to study essential genes and its high specificity. Applications include crop protection and gene therapy. Case studies demonstrate improved insect resistance in transgenic tobacco plants and the role of miRNAs in syncytium formation induced by cyst nematodes.
This presentation is explains about the genome sequencing, its traditional method and modern method. This basically focus on Next Generation Sequencing and its types.
Emulsion PCR is a technique used in next-generation sequencing to amplify DNA sequences attached to beads. It involves compartmentalizing DNA fragments with primer-coated beads into water-in-oil emulsion droplets, with each droplet containing one fragment. The droplets then act as individual PCR reactors to amplify each fragment clonally onto a single bead. After thermal cycling, millions of copies of the DNA fragment are attached to each bead for downstream sequencing applications.
Next generation sequencing (NGS) uses high-throughput technologies to sequence DNA more quickly and cheaply than previous Sanger sequencing. NGS involves library preparation by fragmenting DNA and ligating adaptors, amplification using emulsion PCR or bridge PCR to cluster DNA fragments, and sequencing using different platform-specific methods like pyrosequencing, semiconductor sequencing, or reversible terminator sequencing. These NGS methods allow genome sequencing projects that took years to complete using Sanger to now be finished in just hours.
CRISPR-Cas9 is a powerful gene editing tool that has promising applications in public health. It allows targeted editing of genes and could help treat diseases like HIV/AIDS, cancer, and antibiotic resistance. However, there are also ethical concerns about its use, such as off-target effects and questions around human enhancement. Going forward, CRISPR holds potential for developing new therapies and improving agriculture, but its applications will require addressing safety, consent, and access issues.
This document discusses next generation sequencing technologies. It provides details on several massively parallel sequencing platforms and describes their advantages over traditional Sanger sequencing such as higher throughput, lower costs, and ability to process millions of reads in parallel. It then outlines several applications of next generation sequencing like mutation discovery, transcriptome analysis, metagenomics, epigenetics research and discovery of non-coding RNAs.
CRISPR-Cas9 is a genome editing tool that is creating a buzz in the science world. It is faster, cheaper and more accurate than previous techniques of editing DNA and has a wide range of potential applications.
Point-of-Need Testing: Application of Microfluidic Technologies - 2018 Report...Yole Developpement
Decentralized testing is now widespread, thanks to the endless possibilities enabled by microfluidic technologies.
More information on that report at https://www.i-micronews.com/report/product/point-of-need-testing-application-of-microfluidic-technologies.html
Status of the Microfluidics Industry 2019 report by Yole Développement Yole Developpement
Diversification of microfluidic technologies has led to burgeoning new applications and market growth, driving players’ interest and M&A.
More information on https://www.i-micronews.com/products/status-of-the-microfluidics-industry-2019/
Next-generation sequencing (NGS) has various applications in livestock genetics and breeding including:
1. Whole genome sequencing to identify genetic variations within and between species and quantify introgression.
2. RNA sequencing to detect differentially expressed genes between control and infected/challenged animals and identify genes related to disease resistance.
3. Genome-wide association studies using SNPs identified through NGS to map quantitative trait loci and guide marker-assisted selection for improved traits.
The document summarizes the principle and workflow of Illumina next-generation sequencing. It begins with an overview of Illumina and the development of their sequencing technologies. It then describes the wide range of applications of NGS. The core principle is sequencing by synthesis using reversible dye-terminators. The workflow involves library preparation through fragmentation and ligation of adapters, cluster generation by bridge amplification on a flow cell, and sequencing through cycles of reversible terminator incorporation and imaging. Finally, the sequenced reads are aligned and analyzed using Illumina's data analysis software suite.
Next-generation DNA sequencing technologies have significantly impacted genetics research. Three major platforms - Roche/454, Illumina Genome Analyzer, and Applied Biosystems SOLiD - utilize massively parallel sequencing to generate large amounts of sequence data. Roche/454 uses emulsion PCR to amplify DNA fragments on beads and pyrosequencing to determine sequences. Illumina performs bridge amplification on a flow cell to generate DNA clusters then sequences by synthesis. Applied Biosystems SOLiD uses ligation-based sequencing. These new methods have enabled genome-wide studies and applications such as ancient DNA sequencing and metagenomics that were previously difficult or impossible.
This document discusses DNA sequencing methods, both current and developing technologies. It begins by explaining Sanger sequencing and how fluorescent dyes and thermal cycling improved it. High-throughput short and long-read sequencing methods are then outlined, including Illumina, Ion Torrent, Nanopore, and SMRT sequencing. Developing methods like tunneling currents, hybridization, and microscopy techniques are also mentioned. Overall, the document provides a comprehensive overview of the major DNA sequencing techniques used today and those under investigation.
This document discusses the use of organoid cultures in cancer research. It begins with an overview of what organoids are and provides a simple definition of organoids as miniature organs grown in vitro. The document then discusses how three-dimensional culture techniques have enabled the real-time study of mammalian tissues by allowing independent manipulation of genetic and microenvironmental factors. Examples are provided of different cellular inputs used in 3D cultures as well as different culture formats. The roles of 3D cultures in advancing cancer therapeutics through predictive and prognostic testing of preclinical treatments are also summarized.
CRISPR is easily the best gene editing tool to date. For decades, scientists have been looking for a way to to perform precise changes to genetic sequences. In the past several years, researchers were able to exploit the immune systems of bacteria to edit the genome of other living cells. CRISPR is reported to have higher targeting efficiencies when compared to TALENs and Zinc Fingers. It is efficient, easy to use and cheap; making it a scalable genetic engineering tool that is highly desirable in various industry-wide applications.
Synthetic biology is the design and construction of novel artificial biological pathways, organisms, or devices. This document discusses synthetic biology tools and applications in pathway engineering, with a focus on plants. It provides examples of introducing the artemisinin biosynthesis pathway into tobacco using a combinatorial supertransformation approach, and engineering yeast to produce the sesquiterpene α-santalene. While synthetic biology has potential applications in health, environment and energy, it also faces risks that must be addressed including unintentional release of modified organisms and dual-use concerns.
Next generation sequencing techniques have revolutionized DNA sequencing by increasing throughput and decreasing costs compared to previous methods like Sanger sequencing. Some key next generation sequencing methods include 454 sequencing (pyrosequencing), ABI Solid sequencing (sequencing by ligation), Illumina/Solexa sequencing (sequencing by synthesis), and nanopore sequencing. These new techniques allow for faster and cheaper large-scale sequencing and have enabled applications like whole genome sequencing.
Next generation sequencing (NGS) provides a high-throughput and cheaper alternative to DNA sequencing through massively parallel sequencing of millions of DNA fragments simultaneously. NGS can be used for target sequencing to identify disease-causing mutations, RNA sequencing to study entire transcriptomes, and has various applications in cancer research and treatment including identifying mutations that predict responses to immunotherapy. However, NGS also faces challenges like accurately sequencing regions with repeats and detecting fusion genes.
The document discusses the history and evolution of DNA sequencing techniques. It describes first generation Sanger sequencing and how next generation sequencing (NGS) allows for massively parallel sequencing of entire human genomes in a single day. The principles of NGS involve fragmenting DNA, ligating adaptors, sequencing in parallel, and reassembling the results. Common NGS methods include sequencing by synthesis, pyrosequencing, and ion semiconductor sequencing. Applications of NGS include rapidly sequencing whole genomes, detecting rare mutations, studying gene expression, and analyzing the human microbiome.
Single Nucleotide Polymorphism Analysis
Predictive Analytics and Data Science Conference May 27-28
Asst. Prof. Vitara Pungpapong, Ph.D.
Department of Statistics
Faculty of Commerce and Accountancy
Chulalongkorn University
Production and Purification of Virus Like Particle (VLP) based VaccineDr. Priyabrata Pattnaik
This document summarizes a presentation on the production and purification of virus-like particle (VLP) based vaccines. It discusses using hepatitis C VLPs as a model system produced using an insect cell/baculovirus expression platform. Key points covered include:
- Challenges in VLP vaccine production include low yields, stability issues, and difficulties removing baculovirus.
- Hepatitis C VLPs containing E1 and E2 glycoproteins were successfully produced using Sf9 insect cells in a Mobius 3L disposable bioreactor, with comparable results to a glass bioreactor.
- A depth filtration clarification process achieved around 70% DNA clearance while recovering approximately 70
Role of Antisense and RNAi-based Gene Silencing in Crop ImprovementMariya Zaman
This document presents information on RNA interference (RNAi) and its application in crop improvement. It discusses the discovery of antisense RNA and RNAi technology. The mechanisms of antisense technology and RNAi are described. Advantages of RNAi include its ability to study essential genes and its high specificity. Applications include crop protection and gene therapy. Case studies demonstrate improved insect resistance in transgenic tobacco plants and the role of miRNAs in syncytium formation induced by cyst nematodes.
This presentation is explains about the genome sequencing, its traditional method and modern method. This basically focus on Next Generation Sequencing and its types.
Emulsion PCR is a technique used in next-generation sequencing to amplify DNA sequences attached to beads. It involves compartmentalizing DNA fragments with primer-coated beads into water-in-oil emulsion droplets, with each droplet containing one fragment. The droplets then act as individual PCR reactors to amplify each fragment clonally onto a single bead. After thermal cycling, millions of copies of the DNA fragment are attached to each bead for downstream sequencing applications.
Next generation sequencing (NGS) uses high-throughput technologies to sequence DNA more quickly and cheaply than previous Sanger sequencing. NGS involves library preparation by fragmenting DNA and ligating adaptors, amplification using emulsion PCR or bridge PCR to cluster DNA fragments, and sequencing using different platform-specific methods like pyrosequencing, semiconductor sequencing, or reversible terminator sequencing. These NGS methods allow genome sequencing projects that took years to complete using Sanger to now be finished in just hours.
CRISPR-Cas9 is a powerful gene editing tool that has promising applications in public health. It allows targeted editing of genes and could help treat diseases like HIV/AIDS, cancer, and antibiotic resistance. However, there are also ethical concerns about its use, such as off-target effects and questions around human enhancement. Going forward, CRISPR holds potential for developing new therapies and improving agriculture, but its applications will require addressing safety, consent, and access issues.
This document discusses next generation sequencing technologies. It provides details on several massively parallel sequencing platforms and describes their advantages over traditional Sanger sequencing such as higher throughput, lower costs, and ability to process millions of reads in parallel. It then outlines several applications of next generation sequencing like mutation discovery, transcriptome analysis, metagenomics, epigenetics research and discovery of non-coding RNAs.
CRISPR-Cas9 is a genome editing tool that is creating a buzz in the science world. It is faster, cheaper and more accurate than previous techniques of editing DNA and has a wide range of potential applications.
Point-of-Need Testing: Application of Microfluidic Technologies - 2018 Report...Yole Developpement
Decentralized testing is now widespread, thanks to the endless possibilities enabled by microfluidic technologies.
More information on that report at https://www.i-micronews.com/report/product/point-of-need-testing-application-of-microfluidic-technologies.html
Status of the Microfluidics Industry 2019 report by Yole Développement Yole Developpement
Diversification of microfluidic technologies has led to burgeoning new applications and market growth, driving players’ interest and M&A.
More information on https://www.i-micronews.com/products/status-of-the-microfluidics-industry-2019/
Organs-On-Chips Market and Technology Landscape 2019 report by Yole Développe...Yole Developpement
This report provides an analysis of the organ-on-chip market and technology from 2019-2024. It includes market forecasts for organ-on-chip device sales and services through 2024. It also examines the organ-on-chip ecosystem and supply chain, as well as technology trends like the types of organ models, devices, materials, and cell sources used. The report aims to help pharmaceutical, biotech, and other companies understand organ-on-chip technologies and how they can be used for drug development, disease modeling, and toxicity testing applications.
Emerging Printing Technologies 2019 report by Yole Développement Yole Developpement
Emerging printing technologies are filling the gap for rising applications.
More information on https://www.i-micronews.com/products/emerging-printing-technologies-2019/
This document provides a summary of a patent landscape analysis report on nanopore sequencing technologies. The analysis found that patent publications on nanopore sequencing have significantly increased from 2008-2013, following early work proving the concept. While industrial players are increasingly active, Harvard University's portfolio remains the strongest, followed by Illumina, Agilent, University of California, and Roche. Oxford Nanopore Technologies' portfolio is not the strongest but the company benefits from university partnerships. Several microelectronics companies have also recently filed solid-state nanopore patents. Most major assignees have an international IP strategy with a strong US and European presence. The report provides a detailed analysis of patents segmented by nanopore technology and application, as well as profiles of key
Miniature Drug delivery System uses micro-fabricated devices to administer drugs to the host and has advantages over a conventional drug delivery system
A MEMS based Implantable Drug Delivery System (IDDS) is discussed. The heart of the system is an in-plane MEMS micropumps enables us to make a compact, inexpensive system. A conceptual IDDS design is proposed. This design consists of an implantable unit which houses the micropumps, electronic and power circuitry. This implantable unit is connected to a subcutaneous port via a silicone catheter. The subcutaneous port acts as a refillable reservoir. This leads to a reduction in unit volume and makes the system customizable. The IDDS pumps drug into surrounding tissue with the help of a MEMS based micropumps. Similarly Microneedles are also used in drug administration.
This report gives an overview of patenting activity around Miniature Drug Delivery System. Smart drug delivery system is used for delivering drugs to the host effectively and improves the quality of life of the patients. Patents were categorized as per key Drug Delivery administration methods, types of sensors, applications, communication techniques and analyzed for generating different trends with Patent iNSIGHT Pro.
Miniature Drug Delivery System technology has seen a consistent increase in the number of patent publications from 2008 till 2012 as the need for smart drug delivery has increase globally.
Published: Apr 30, 2013
Cameras for Microscopy and Next-Generation Sequencing 2019 report by Yole Dév...Yole Developpement
Disposable image sensors: a revolution for microscopy and next-generation sequencing.
More information on: https://www.i-micronews.com/products/cameras-for-microscopy-and-next-generation-sequencing-2019/
Chinese Microfluidics Industry 2018 Report by Yole DeveloppementYole Developpement
Will the Chinese microfluidics industry change the worldwide microfluidic landscape?
More information on that report at: https://www.i-micronews.com/category-listing/product/chinese-microfluidics-industry-2018.html
Ultrasound Sensing Technologies for Medical, Industrial and Consumer Applicat...Yole Developpement
New applications along with manufacturing capabilities and technological readiness are driving the takeoff of micro-machined ultrasonic transducers.
More information on that report at https://www.i-micronews.com/category-listing/product/ultrasound-sensing-technologies-for-medical-industrial-and-consumer-applications.html
Global Next Generation Sequencing (NGS) Industry By Market Size & Forecast to...DavidClark206
This research report covers end-to-end market for NGS in terms of the workflow; presequencing, NGS platforms, consumables and services, sequencing services and bioinformatics market. From an insight perspective, this research report focuses on the qualitative data, market size, share, and growth of various segments and sub-segments, competitive landscape, and company profiles.
Inquire For Discount (Single User Report Price US $4650) @ http://www.reportsnreports.com/contacts/Discount.aspx?name=257153 .
COVID-19 is shaking up the diagnostics industry and will have both short- and long-term impact.
More information: https://www.i-micronews.com/products/point-of-need-2020-including-pcr-based-testing/
The document discusses strategies for seed companies to gain strategic leadership in technology. It covers understanding trends, technology management functions, strategic planning, technology access issues, intellectual property implications, and approaches to consolidation. The key points are that vision and strategic planning are essential to technology leadership, understanding economic and market trends is important for priority setting, and both organic and inorganic growth through research, licensing, and partnerships/acquisitions can help companies accelerate innovation.
CRISPR Technology & Market Overview: from Lab to Industry 2018 Report by Yol...Yole Developpement
CRISPR-based products in biotech, agritech and diagnostics markets will reach $5B in 2023 before extra growth comes from therapeutic applications.
More information on that report at https://www.i-micronews.com/category-listing/product/crispr-technology-market-overview-from-lab-to-industry-2018.html
Point-of-Need Testing: Application of Microfluidic Technologies - 2016 Report...Yole Developpement
Decentralized testing for both human and non-human in-vitro diagnostics is increasingly taking advantage of innovative microfluidic technologies
PLENTY OF APPLICATIONS HAVE STARTED TO BENEFIT FROM MICROFLUIDICS FOR DECENTRALIZED TESTING, BUT HAVE NOT REALIZED THEIR FULL POTENTIAL – YET
Point-of-Care (PoC) testing is not a new concept - the first applications arose in the 1990s. Since then, microfluidic technologies have been increasingly used to solve technical problems and bring economic benefits to the healthcare industry. In the past few years, other applications have benefited from recent technological improvements: veterinary testing, environmental testing, agro-food and industrial testing are also part of the scope of the report. Decentralized testing brings significant operational benefits to various players across these applications.
Microfluidics is part of the diagnostics revolution, with an explosion in the number of products on the market. However, with many applications but few solutions existing today, these markets have not yet reached their full potential.
In the report, Yole Développement (Yole)’s analysts explain why the human healthcare market still looks much more attractive to technology developers. However, in the near future the greatest opportunities will be in all the other markets. The report also explores which barriers still need to be taken down for decentralized, or “point-of-need” (PoN), testing to flourish.
Microfluidics-based point-of-need testing will grow from a $2.6B market in 2015 to $10.3B in 2021, which is a 26% compound annual growth rate (CAGR). This value represents more than 500 million tests in 2021, but only 61 million will be outside human diagnostics. In the report, Yole’s analysts detail the evolution of each application in terms of microfluidic technology’s use.
Nanotechnology involves manipulating matter at the nanoscale (1-100nm) to utilize size-dependent properties. Global investment in nanotechnology R&D was $40 billion in 2008 and $41 billion in 2010, with expected growth areas including healthcare, pharmaceuticals, and energy. Nanotechnology converges with many disciplines and may impact sectors like health, IT, and energy through applications in areas such as diagnostics, drug delivery, and renewable energy. Realizing nanotechnology's potential will require addressing challenges like health and environmental risks, intellectual property issues, and public acceptance.
The document discusses business intelligence services provided by AREA Science Park to help companies in Friuli Venezia Giulia region. The services include monitoring emerging technologies using tools from SRI Consulting to identify trends, support business decisions, and identify new opportunities. Specifically, Explorer is offered, which provides reports on over 30 technology sectors to help evaluate technology competitiveness, opportunities, threats, and commercialization potential. The services aim to help small and medium companies, project evaluators, and education providers.
BioMEMS & Non-Invasive Sensors: Microsystems for Life Sciences & Healthcare 2...Yole Developpement
A new wave of sensors, responding to the challenge of global healthcare transformation, opens new business opportunities for mobile healthcare and emerging non-invasive devices.
More information on that report at https://www.i-micronews.com/category-listing/product/biomems-non-invasive-sensors-microsystems-for-life-sciences-healthcare-2018.html#table-of-contents
3D Cell Culture technologies Patent Landscape Sample 2016 Knowmade
The patent landscape related to 3D cell technologies is very open, involving important academic applicants as well as small companies. It includes over 2,500 patent families and involves over 1,000 patent applicants.
The report provides essential patent data for 3D cell culture technologies including:
• Time evolution of patent publications and countries of patent filings
• Current legal status of patents
• Ranking of main patent applicants
• Joint developments and IP collaboration network of main patent applicants
• Key patents
• Granted patents near expiration.
• Relative strength of main companies IP portfolio
• 3D cell culture IP profiles of 10+ major companies with key patents, partnerships, and IP strength and strategy
In the ultrasound module market, CMUT and PMUT are growing two times faster in medical and consumer applications.
More information: https://www.i-micronews.com/products/ultrasound-sensing-technologies-2020/
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Strong momentum for MicroLED with progress on all fronts. Cost is the biggest challenge, but Apple and Samsung are carving paths toward the consumer.
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System-in-Package Technology and Market Trends 2021 - SampleYole Developpement
Through enabling design and supply chain agility, SiP will reach $19B by 2026, with IDMs, OSATs, and foundries taking advantage of it.
More information : https://www.i-micronews.com/products/system-in-package-technology-and-market-trends-2021/
Industrial, consumer, and automotive applications are driving the adoption of neuromorphic computing and sensing technologies. The first products are now hitting the market.
More information: https://www.i-micronews.com/products/neuromorphic-computing-and-sensing-2021/
Beyond communication, silicon photonics is penetrating consumer and automotive – heading to $1.1B in 2026.
More information: https://www.i-micronews.com/products/silicon-photonics-2021/
Semiconductor technologies will enable increased mobility and communication for the soldier of the future. This market will reach $17.5B in 2030+.
More information: https://www.i-micronews.com/products/future-soldier-technologies-2021/
This report from Yole Développement analyzes the high-end performance packaging market. It defines high-end performance packaging as technologies that provide high IO density (≥16/mm2) and fine IO pitch (≤130μm). The report aims to identify relevant technologies, analyze market drivers and challenges, describe technology trends and roadmaps, examine the supply chain landscape, and provide market forecasts. It evaluates the market by technology, end application, and region. The report also profiles key players' technology roadmaps and analyzes intellectual property in the 3D SoC hybrid bonding space.
5G’s Impact on RF Front-End and Connectivity for Cellphones 2020Yole Developpement
An intensifying US-China competition for RF technology supremacy.
More information on: https://www.i-micronews.com/products/5gs-impact-on-rf-front-end-and-connectivity-for-cellphones-2020/
The entrance of Chinese players and the rise of new technical solutions are poised to trigger profound changes in the memory business.
More information on: https://www.i-micronews.com/products/status-of-the-memory-industry-2020/
GaAs Wafer and Epiwafer Market: RF, Photonics, LED, Display and PV Applicatio...Yole Developpement
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Status of the Radar Industry: Players, Applications and Technology Trends 2020Yole Developpement
Worth more than $20B in 2019, the radar industry is experiencing a major transformation prior to entering the commercial era.
Learn more about the report here: https://www.i-micronews.com/products/status-of-the-radar-industry-players-applications-and-technology-trends-2020/
GaN RF Market: Applications, Players, Technology and Substrates 2020Yole Developpement
Driven by military applications and 5G telecom infrastructure, the GaN RF market continues growing.
Learn more about the report here: https://www.i-micronews.com/products/gan-rf-market-applications-players-technology-and-substrates-2020/
Pressure, inertial, MEMS ultrasound, microfluidic chips and other sensors are driving the growth of the life sciences and healthcare market.
More information: https://www.i-micronews.com/products/biomems-market-and-technology-2020/
Market will more than double by 2025 driven by heavy investments in data centers.
More information: https://www.i-micronews.com/products/optical-transceivers-for-datacom-telecom-2020/
Pluggable transceivers in high volume production. Co-packaged optics in line of sight.
More information on: https://www.i-micronews.com/products/silicon-photonics-2020/
The one million robotic vehicle milestone will be reached by end of the decade: The industrial phase has been launched.
More information on: https://www.i-micronews.com/products/sensors-for-robotic-mobility-2020/
High-End Inertial Sensors for Defense, Aerospace and Industrial Applications ...Yole Developpement
High-end inertial sensors are the backbone of systems that will enable autonomous transportation and the new space industry.
More information on: https://www.i-micronews.com/products/high-end-inertial-sensors-for-defense-aerospace-and-industrial-applications-2020/
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I develop the Ruby programming language, RubyGems, and Bundler, which are package managers for Ruby. Today, I will introduce how to enhance the security of your application using open-source software (OSS) examples from Ruby and RubyGems.
The first topic is CVE (Common Vulnerabilities and Exposures). I have published CVEs many times. But what exactly is a CVE? I'll provide a basic understanding of CVEs and explain how to detect and handle vulnerabilities in OSS.
Next, let's discuss package managers. Package managers play a critical role in the OSS ecosystem. I'll explain how to manage library dependencies in your application.
I'll share insights into how the Ruby and RubyGems core team works to keep our ecosystem safe. By the end of this talk, you'll have a better understanding of how to safeguard your code.
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The videorecording (in Czech) from the presentation is available here: https://youtu.be/WzjJWm4IyPk?si=SImb06tuXGb30BEH .
5th LF Energy Power Grid Model Meet-up SlidesDanBrown980551
5th Power Grid Model Meet-up
It is with great pleasure that we extend to you an invitation to the 5th Power Grid Model Meet-up, scheduled for 6th June 2024. This event will adopt a hybrid format, allowing participants to join us either through an online Mircosoft Teams session or in person at TU/e located at Den Dolech 2, Eindhoven, Netherlands. The meet-up will be hosted by Eindhoven University of Technology (TU/e), a research university specializing in engineering science & technology.
Power Grid Model
The global energy transition is placing new and unprecedented demands on Distribution System Operators (DSOs). Alongside upgrades to grid capacity, processes such as digitization, capacity optimization, and congestion management are becoming vital for delivering reliable services.
Power Grid Model is an open source project from Linux Foundation Energy and provides a calculation engine that is increasingly essential for DSOs. It offers a standards-based foundation enabling real-time power systems analysis, simulations of electrical power grids, and sophisticated what-if analysis. In addition, it enables in-depth studies and analysis of the electrical power grid’s behavior and performance. This comprehensive model incorporates essential factors such as power generation capacity, electrical losses, voltage levels, power flows, and system stability.
Power Grid Model is currently being applied in a wide variety of use cases, including grid planning, expansion, reliability, and congestion studies. It can also help in analyzing the impact of renewable energy integration, assessing the effects of disturbances or faults, and developing strategies for grid control and optimization.
What to expect
For the upcoming meetup we are organizing, we have an exciting lineup of activities planned:
-Insightful presentations covering two practical applications of the Power Grid Model.
-An update on the latest advancements in Power Grid -Model technology during the first and second quarters of 2024.
-An interactive brainstorming session to discuss and propose new feature requests.
-An opportunity to connect with fellow Power Grid Model enthusiasts and users.
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Ever-changing customer expectations demand more modern digital experiences, and the bank needed to find a solution that could provide real-time data to its customer channels with low latency and operating costs. Join this session to learn how Citizens is leveraging Precisely to replicate mainframe data to its customer channels and deliver on their “modern digital bank” experiences.
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Wir erklären Ihnen, wie Sie häufige Konfigurationsprobleme lösen können, die dazu führen können, dass mehr Benutzer gezählt werden als nötig, und wie Sie überflüssige oder ungenutzte Konten identifizieren und entfernen können, um Geld zu sparen. Es gibt auch einige Ansätze, die zu unnötigen Ausgaben führen können, z. B. wenn ein Personendokument anstelle eines Mail-Ins für geteilte Mailboxen verwendet wird. Wir zeigen Ihnen solche Fälle und deren Lösungen. Und natürlich erklären wir Ihnen das neue Lizenzmodell.
Nehmen Sie an diesem Webinar teil, bei dem HCL-Ambassador Marc Thomas und Gastredner Franz Walder Ihnen diese neue Welt näherbringen. Es vermittelt Ihnen die Tools und das Know-how, um den Überblick zu bewahren. Sie werden in der Lage sein, Ihre Kosten durch eine optimierte Domino-Konfiguration zu reduzieren und auch in Zukunft gering zu halten.
Diese Themen werden behandelt
- Reduzierung der Lizenzkosten durch Auffinden und Beheben von Fehlkonfigurationen und überflüssigen Konten
- Wie funktionieren CCB- und CCX-Lizenzen wirklich?
- Verstehen des DLAU-Tools und wie man es am besten nutzt
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- Praxisbeispiele und Best Practices zum sofortigen Umsetzen
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This slide deck presents DLHT, a concurrent in-memory hashtable. Despite efforts to optimize hashtables, that go as far as sacrificing core functionality, state-of-the-art designs still incur multiple memory accesses per request and block request processing in three cases. First, most hashtables block while waiting for data to be retrieved from memory. Second, open-addressing designs, which represent the current state-of-the-art, either cannot free index slots on deletes or must block all requests to do so. Third, index resizes block every request until all objects are copied to the new index. Defying folklore wisdom, DLHT forgoes open-addressing and adopts a fully-featured and memory-aware closed-addressing design based on bounded cache-line-chaining. This design offers lock-free index operations and deletes that free slots instantly, (2) completes most requests with a single memory access, (3) utilizes software prefetching to hide memory latencies, and (4) employs a novel non-blocking and parallel resizing. In a commodity server and a memory-resident workload, DLHT surpasses 1.6B requests per second and provides 3.5x (12x) the throughput of the state-of-the-art closed-addressing (open-addressing) resizable hashtable on Gets (Deletes).
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Monitoring and Managing Anomaly Detection on OpenShift.pdfTosin Akinosho
Monitoring and Managing Anomaly Detection on OpenShift
Overview
Dive into the world of anomaly detection on edge devices with our comprehensive hands-on tutorial. This SlideShare presentation will guide you through the entire process, from data collection and model training to edge deployment and real-time monitoring. Perfect for those looking to implement robust anomaly detection systems on resource-constrained IoT/edge devices.
Key Topics Covered
1. Introduction to Anomaly Detection
- Understand the fundamentals of anomaly detection and its importance in identifying unusual behavior or failures in systems.
2. Understanding Edge (IoT)
- Learn about edge computing and IoT, and how they enable real-time data processing and decision-making at the source.
3. What is ArgoCD?
- Discover ArgoCD, a declarative, GitOps continuous delivery tool for Kubernetes, and its role in deploying applications on edge devices.
4. Deployment Using ArgoCD for Edge Devices
- Step-by-step guide on deploying anomaly detection models on edge devices using ArgoCD.
5. Introduction to Apache Kafka and S3
- Explore Apache Kafka for real-time data streaming and Amazon S3 for scalable storage solutions.
6. Viewing Kafka Messages in the Data Lake
- Learn how to view and analyze Kafka messages stored in a data lake for better insights.
7. What is Prometheus?
- Get to know Prometheus, an open-source monitoring and alerting toolkit, and its application in monitoring edge devices.
8. Monitoring Application Metrics with Prometheus
- Detailed instructions on setting up Prometheus to monitor the performance and health of your anomaly detection system.
9. What is Camel K?
- Introduction to Camel K, a lightweight integration framework built on Apache Camel, designed for Kubernetes.
10. Configuring Camel K Integrations for Data Pipelines
- Learn how to configure Camel K for seamless data pipeline integrations in your anomaly detection workflow.
11. What is a Jupyter Notebook?
- Overview of Jupyter Notebooks, an open-source web application for creating and sharing documents with live code, equations, visualizations, and narrative text.
12. Jupyter Notebooks with Code Examples
- Hands-on examples and code snippets in Jupyter Notebooks to help you implement and test anomaly detection models.
Your One-Stop Shop for Python Success: Top 10 US Python Development Providersakankshawande
Simplify your search for a reliable Python development partner! This list presents the top 10 trusted US providers offering comprehensive Python development services, ensuring your project's success from conception to completion.
22. DNA sequencing has many applications in
healthcare and life sciences and it is increasingly
used in myriad areas as cost reduction starts to
permit it. Research is progressing and enabling a
better understanding of the genetic content of
life. Sequencing therefore holds a bright future
in clinical applications, enabling clinical decisions
on the basis of sequencing information. Increasing
adoption paves the way for personalized medicine,
helping to better understand cancers and rare
genetic diseases. Sequencing even has potential
for non-invasive early cancer detection with
liquid biopsies. It is also increasingly used in
other applications, such as forensics, agricultural
sciences and drug development. Furthermore,
the possibility of encoding vast amounts of
information in DNA could replace our current
data storage solutions, offering an insanely space
efficient storage at low cost in the future.
The sequencing market is dominated by a handful of
players, all with their own sequencing technology,
and associated advantages and drawbacks.
However, Illumina currently holds more than 80%
of the sequencing market, leaving only crumbs to
its competitors. Nevertheless, newcomers such
as China’s BGI and the UK’s Oxford Nanopore
have the potential to change this along with other
technologies in development, some of which
might reach the market as soon as 2019. In total,
Yole Developpement’s (Yole) analysts identified
more than 50 companies developing sequencing
technologies. In the report, Yole's analysts provide
our analysis of how the sequencing landscape
will evolve in the coming years and how these
new technologies could change the game. Cost,
throughput, read length, accuracy, speed, portability,
ease of interpretation are criteria on which there is
room for improvement, and Illumina might win some
but not all of these races.
One thing remains clear: we are still in the early
days of sequencing and tremendous growth is
expected as the use of sequencing spreads. This
is why Yole’s analysts expect that the fleet of
sequencing instruments will more than double by
2024, from almost 30,000 sequencers today. As
a consequence of the razor/razor-blade business
model of sequencing consumables, the number of
sequencing flow cells, the disposable chips used
to perform and sometimes detect the sequencing
reaction, is poised to grow at a 21% Compound
Annual Growth Rate (CAGR), from 1.28 million
units in 2018 to 4.19 million units in 2024. But
this is only a first step: the $1,000 genome was
achieved years ago, and we’re now heading
towards the $100 genome. The continuous
improvement of sequencing technologies will
someday lead to a much more affordable and
practical sequencing, the $10 genome or even
below. At this point, it is not millions of flow cells
per year but hundreds of millions that one needs
to consider, representing an immense opportunity
for the entire semiconductor supply chain.
NEXT GENERATION SEQUENCING & DNA SYNTHESIS:
TECHNOLOGY, CONSUMABLES MANUFACTURING
AND MARKET TRENDS 2019
Market & Technology Report - April 2019
With the sequencer installed base doubling,the sequencing consumables market will reach $7.8B by 2024.
REPORT KEY FEATURES
• History of DNA sequencing
• Use of semiconductor technologies
in sequencing
• Market data, forecasts and market
shares including installed base of
sequencers, number of flow cells,
number of 8” equivalent wafers,
consumables market in $, fab market
in $ and raw material market in $
• Market analysis: applications, drivers,
competitive landscape, etc.
• Supply chain analysis
• Technology analysis: description,
segmentation and comparison of
sequencing technologies available
and in development. Sample
preparation, detection methods,
focus on semiconductor-based
consumables
• Company profiles: 50+ companies
developing sequencing technologies
• Dedicated part for DNA synthesis
technologies, applications and
market trends with a focus on
semiconductor technologies
REPORT OBJECTIVES
• Give an overview of the Next-
Generation Sequencing and
Next-Generation DNA synthesis
market and technology landscapes,
understand who the established and
upcoming players are, what their
technologies are along with their
advantages and drawbacks, and how
they will evolve in the next few years
• Understand how and why
semiconductor technologies are
leveraged in these fields, enabling
improvements areas such as cost,
size, speed, production scale-up and
reproducibility
• Describe the supply chain
• Identify where the opportunities are
for the different players all along the
supply chain, from materials suppliers
and MEMS/microfluidic foundries to
sequencing technology developers
• Provide detailed market data and
forecasts along with market shares
• Understand the different
technologies at the consumable
flow cell level, in terms of
microstructures, materials and
manufacturing processes (Yole Développement,April 2019)
Sequencing market forecasts of flow cells shipped
SEQUENCING IS A VERY DYNAMIC MARKET AND HAS APPLICATIONS IN
MANY FIELDS, HOWEVER IT IS ONLY THE BEGINNING OF THE STORY
0
1
2
3
4
5
2017 2018 2019 2020 2021 2022 2023 2024
Flowcells(inmillionunitsshipped)
Others
Pacific Biosciences
BGI
Thermo Fisher Scientific
Oxford Nanopore
Illumina
CAGR 2019-2024
= +21%
23. NEXT GENERATION SEQUENCING DNA SYNTHESIS – TECHNOLOGY, CONSUMABLES MANUFACTURING AND MARKET TRENDS 2019
THE TIME FOR NEW DNA SYNTHESIS TECHNOLOGIES BASED ON
SEMICONDUCTOR CHIPS HAS ARRIVED
Gene synthesis technology has revolutionized both
the understanding of DNA functions and the ability
to manipulate DNA for experimental, medical,
and industrial purposes. Until recently, DNA was
synthesized using enzymatic and chemical processes.
These methods include random errors, making the
DNA useless, and finding error-free DNA is a time-
consuming mission requiring further analysis and
sequencing.
Semiconductor chips are allowing important
advancements in the field of genomic research by
enabling the synthesis of thousands of genes in parallel.
This is revolutionizing the way DNA synthesis is
performed, enablingfasterandmoreefficientsynthesis.
Such technology leverages semiconductor processing
techniques to greatly increase throughput and has the
potential to make oligonucleotide synthesis cost 1000
times lower. On the other hand, the throughput can
be increased even further by scaling down the chips’
feature size. In this context, Yole’s analysts estimate
that the global market of semiconductor chips used
for DNA synthesis will reach $213.2M in 2024 with a
CAGR of 40% over the period 2018-2024.
A wide range of applications such as drug discovery,
agriculture and data storage will increasingly rely upon
gene synthesis to solve problems related to healthcare,
food supplies and storage modalities respectively. In
the report, Yole’s analysts explore the possibilities
envisioned thanks to emerging semiconductor-based
DNA synthesis technologies, identify the key players
leading the market, highlight the new needs for each
application and discuss the technology’s evolution.
Sequencing flow cells market shares per material (in units)
(Yole Développement,April 2019)
Glass flow cells
Silicon flow cells
72%
(0.92 million
units)
1.28 million flow cells
2018
2024
*CAGR2018-2024
= +21%
*CAGR: Compound Annual Growth Rate
52%
(2.17 million units)
4.18 million flow cells
28%
(0.36 million
units)
48%
(2.01 million units)
CHEAPER, BETTER, FASTER, LONGER SEQUENCING READS: SEMICONDUCTOR
TECHNOLOGIES HAVE A KEY ROLE TO PLAY IN DRIVING THIS ENDLESS RACE
Most sequencing technologies are using, to some
extent, microstructures. Beads, wells, membranes,
patterned surfaces and nanopores are all examples
of microstructures that can be found in sequencing
consumables. Sequencing consumables from most
players leverage semiconductor technologies to
manufacture these microstructures in a reproducible
and scalable manner. The key to scaling sequencing
power is always increasing flow cells’ density to
generate more data on the same surface area. It is no
coincidence that the cost of sequencing has dropped
much faster than Moore’s law over the past 15 years.
While glass is often used when optical detection
is the method of choice, the advent of electrical
detection methods has led to the increased use of
silicon and CMOS substrates. Silicon, especially
CMOS, is the material of choice for the flow cells
used by most technologies currently in development.
The expected market introduction of several of these
technologies in the next few years will boost the
associated number of wafers. Sequencing companies
need, or will need, to mass-manufacture these flow
cells, creating opportunities at all the levels of the
semiconductor supply chain. Indeed, most sequencing
players have made the choice to outsource this
production to specialized players. However, some
start-ups struggle in the development phase because
foundries seem not to realize the potential volumes
sequencing flow cells could represent in the future.
Consequently it is difficult for them to get access to
the equipment and processes they need. In this report,
Yole analyzes the supply chain and also focuses on
several leading and emerging flow cell technologies
from key and upcoming players including the type of
microstructures, process flow, and evolution over
time. Yole's analysts also talk about the limitation
of optical detection technologies leading to ever
larger glass flow cells, which represents a significant
opportunity for glass players as well.
History of DNA writing
(Yole Développement,April 2019)
1970 1975 1980 1985 1990 1995 2000 2005 2010 2015
100
1,000
10,000
100,000
100,00,000
10,00,000
1,00,000
77bpAgarwal:alatRNA
33bpKoester:angiotensinII
41bpItakura:somatostatin
Constructsize(bp)
514bpEdge:leukocyteinterferon
2.1kbYoung:plasmid
2.7kbStemmer:plasmid
7.5kbCello:poliovirus
32kbKodumal:polyketidesynthase
1.1MbGibson:M.mycoides
273kbAnnaluru:Sc2.0synIII
12MbSc2.0
PCR invention
Introduction of
commercial gene
synthesis
2020
The next generation
of DNA synthesis
Up to ~ 1M Oligos/Chip
Courtesy of Fisher Scientific
24. MARKET TECHNOLOGY REPORT
COMPANIES CITED IN THE REPORT (non exhaustive list)
AGC [Asahi Glass Co.], Agilent Technologies, Advanced Liquid Logic (Illumina), Apton Biosystems,
Armonica Technologies, Atum, Base4 Innovation, BGI [Beijing Genomics Institute], Bio Basic,
Biocat, Bioneer, Bio-Rad Laboratories, Biosearch Technologies, Caerus Molecular Diagnostics,
Catalog, Centrillion Technologies, Complete Genomics (BGI), Corning, Cygnus Biosciences,
Depixus, Desktop Genetics, Direct Genomics, DNA Electronics, DNA Script, Electronic
Biosciences, Electron Optica, Electroseq, Eurofins, Eve Biomedical, Evonetix, Gen9 Bio (Gingko
Bioworks), Genapsys, GeneArt, GeneOracle, Geneseque, Genewiz (Brooks Automation), Genia
Technologies, Genome Surveilllance, GenScript, Gingko Bioworks, GnuBio (Bio-Rad), Grail,
Helixworks, Heraeus, Hoya, Integrated DNA Technologies (Danaher), Illumina, IMT AG, IMT
MEMS, iNanoBio, inSilixa, Intelligent Biosystems (Qiagen), Invenios, Ion Torrent (Thermo Fisher),
Iridia, Jilin Zixin Pharma, Kilobaser, LabGenius, LaserGen (Agilent), Lightspeed Genomics, Little
Things Factory, MGI (BGI), Micralyne, Micronit, Mir Enterprises, Molecular Assemblies, Nabsys,
NanoString Technologies, NorthShore Bio, Novati (Skorpios Technologies), Ohara Corporation,
Omniome, Origene, Oxford Nanopore Technologies [ONT], Pacific Biosciences (Illumina),
Plan Optik, Personal Genomics, Powerchip Technologies, Quantapore, Qiagen, QuantuMDx,
QuantumSi, Roche, Roswell Biotechnologies, Schott AG, SeqLL, Silex Microsystems, SingularBio,
Singular Genomics, Solexa (Illumina), Stratos Genomics, Synthomics, Thermo Fisher Scientific,
TSMC [Taiwan Semiconductor Manufacturing Company], Twist Bioscience, Two Pore Guys,
Universal Sequencing Technology Corporation, Xgenomes, ZS Genetics, and more.
RELATED REPORTS
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Find all our reports on www.i-micronews.com
Sébastien Clerc works as a Technology
Market Analyst, Microfluidics Medical
TechnologiesatYoleDéveloppement(Yole).
As part of the Life Sciences Healthcare
division, Sébastien has authored a collection
of market and technology reports dedicated
to topics such as microfluidics, point-of-
care, MEMS for healthcare applications
and connected medical devices. In parallel,
he is daily involved in custom projects such
as strategic marketing, technology scouting
and technology evaluation to help academic
and industrial players in their innovation
processes. Thanks to his technology
market expertise, Sébastien has spoken
in more than 10 industry conferences
worldwide over the last 2 years. Sébastien
Clerc graduated from Grenoble Institute
of Technology (Grenoble INP - Grenoble,
France) with a Master’s degree in Biomedical
Technologies. He then completed his cursus
with a Master’s degree in Innovation and
Technology Management in the same
institute.
AUTHORS
TABLE OF CONTENTS (complete content on i-Micronews.com)
Executive summary 14
DNA SEQUENCING PART 55
Context 56
Market forecasts 70
Installed base of sequencers
Flow cell market (units and 8” equivalent wafers)
Sequencing consumable market ($M)
Sequencing consumable fab market ($M)
Sequencing consumable raw material market ($M)
Market trends 83
Applications of NGS
Evolution of sequencing revenue by application
Applications driving the use of NGS
Leveraging semiconductor technologies
The value will switch from the optics to the chip
Clonal vs. single molecule technologies – A difficult
road to market
Consumables and sample preparation
Penetration of the clinical market
Why has Illumina won the sequencing race so far?
Why will Illumina be challenged at some point?
Competition on price
Advice to newcomers
Market shares and supply chain 104
Installed base of sequencers
Number of flow cells per player and per material
Potential sequencing output per player
Sequencing consumable revenue per player and per
flow cell material
8” equivalent wafers, per flow cell material
Flow cell fab market, per flow cell material
Flow cell raw material market, per flow cell material
Comments and conclusion
Supply chain analysis:
- DNA sequencing flow cell manufacturing
- Glass substrate providers
Technology trends 124
Methods for DNA sequencing
Clonal amplification vs. single molecule technologies
Sample preparation
Drivers for technology development: an endless race
Clonal vs. single molecule technologies – which ones
are solid-state?
Which substrates are used?
Detection methods
Use of semiconductor substrates
Size of the chips
Flow cell cost and price breakdown
Quality scores in sequencing
Performances comparison of sequencing technologies
Technology focus: Illumina, Oxford Nanopore, Pacific
Biosciences, Ion Torrent
Conclusion
Profiles of over 50 companies developing
sequencing technologies 161
DNA SYNTHESIS PART 193
Definition
Rapid growth in base pairs
A massive divergence in capacity
History of DNA writing
What can be synthesized?
How can DNA be synthesized?
Current DNA synthesis methods
Geographical location of DNA synthesis companies
Mergers and acquisitions
Market data and forecasts
Writing synthetic DNA on silicon chips
Company profiles
Applications for DNA synthesis
New types of DNA suppliers
Market opportunities and current developments
Adoption of silicon chip-based DNA synthesis technology
Conclusion of the report 215
As a Technology Market Analyst,
Biotechnologies Molecular Innovations,
Medical Technologies in the Life Sciences
Healthcare division at Yole Développement
(Yole), Asma Siari is involved in the
development of technology market
reports as well as the production of custom
consulting projects. After a Master’s degree
in Biotechnologies, Diagnostic Therapeutics
Management, Asma served as Research
Assistant at the Moores Cancer Center
(San Diego, CA). She is a coauthor in
three scientific publications published in
the Molecular Cancer Research Journal.
Asma Siari graduated with an Advanced
Master’s degree in International Strategy
Marketing BtoB from EM Lyon Business
School (France).
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