The oral presentation at Congress of the Italian Association of Veterinary Laboratory Diagnosticians.
Staphylococcus aureus is the most common cause of food poisoning worldwide. Enterotoxigenic strains can produce multiple staphylococcal enterotoxins (SEs), including the five major Ses: SEA, SEB, SEC, SED, and SEE. While variants of these toxins have been described and were linked to specific hosts or levels or enterotoxin production, data on sequence variation is still limited. In this study, we analysed the promoter and gene sequences of seb, sec, and sed from strains isolated from foodborne outbreaks, human infections, human nasal colonization, rabbits, and cattle. New sequence variants were detected for all three enterotoxins. While the seb promoter and gene sequences exhibited a high degree of variability, the sec and sed promoter and gene were more conserved. Interestingly, a truncated variant of sed was detected in all tested sed harboring rabbit strains.
Dr. Yao-Wei Huang - Here we go again? Emergence of a novel swine enteric alph...John Blue
Here we go again? Emergence of a novel swine enteric alphacoronavirus (SeACov) in Southern China - Dr. Yao-Wei Huang, Zhejiang University, from the 2017 North American PRRS/National Swine Improvement Federation Joint Meeting, December 1‐3, 2017, Chicago, Illinois, USA.
More presentations at http://www.swinecast.com/2017-north-american-prrs-nsif-joint-meeting
Dr. Yao-Wei Huang - Here we go again? Emergence of a novel swine enteric alph...John Blue
Here we go again? Emergence of a novel swine enteric alphacoronavirus (SeACov) in Southern China - Dr. Yao-Wei Huang, Zhejiang University, from the 2017 North American PRRS/National Swine Improvement Federation Joint Meeting, December 1‐3, 2017, Chicago, Illinois, USA.
More presentations at http://www.swinecast.com/2017-north-american-prrs-nsif-joint-meeting
Transfer of Potential pseudomonas stutzeri genes ROB1323
In this research of saxitoxin bacterial production bioinformatics will be utilized to identify sxt coding genes within pseudomonas stutzeri strain A1501. PCR will then be employed in the isolation of sxt genes from pseudomonas stutzeri. The isolated genes will be transformed into Ecoli cultures.
Transfer of Potential pseudomonas stutzeri genes ROB1323
In this research of saxitoxin bacterial production bioinformatics will be utilized to identify sxt coding genes within pseudomonas stutzeri strain A1501. PCR will then be employed in the isolation of sxt genes from pseudomonas stutzeri. The isolated genes will be transformed into Ecoli cultures.
Shigella flexneri serotype 1c derived from serotype 1a by acquisition of gtrI...Swee Seong TANG
Background
Shigella spp. are the primary causative agents of bacillary dysentery. Since its emergence in the late 1980s, the S. flexneri serotype 1c remains poorly understood, particularly with regard to its origin and genetic evolution. This article provides a molecular insight into this novel serotype and the gtrIC gene cluster that determines its unique immune recognition.
Results
A PCR of the gtrIC cluster showed that serotype 1c isolates from different geographical origins were genetically conserved. An analysis of sequences flanking the gtrIC cluster revealed remnants of a prophage genome, in particular integrase and tRNAPro genes. Meanwhile, Southern blot analyses on serotype 1c, 1a and 1b strains indicated that all the tested serotype 1c strains may have had a common origin that has since remained distinct from the closely related 1a and 1b serotypes. The identification of prophage genes upstream of the gtrIC cluster is consistent with the notion of bacteriophage-mediated integration of the gtrIC cluster into a pre-existing serotype.
Conclusions
This is the first study to show that serotype 1c isolates from different geographical origins share an identical pattern of genetic arrangement, suggesting that serotype 1c strains may have originated from a single parental strain. Analysis of the sequence around the gtrIC cluster revealed a new site for the integration of the serotype converting phages of S. flexneri. Understanding the origin of new pathogenic serotypes and the molecular basis of serotype conversion in S. flexneri would provide information for developing cross-reactive Shigella vaccines.
Keywords
Shigella flexneri, Bacillary dysentery, Serotype-conversion , Evolutionary origin, Glucosyltransferase, Serotype 1c
Since Staphylococcus nepalensis were reported for the first time from Nepalese animal specimen, and have been reported from human specimens elsewhere, this bug can be a threat in our part. Protocols must be designed aimed at their identification in our laboratory during microbiological analysis of clinical specimens.
Classification of enterbacteriaceae and biochemical reactionsDAKhan4
slide 2
Commonly present in large intestine •
Non sporing ,
Non Acid fast,
Gram – bacilli. •
A complex family of organisms, •
Some are non pathogenic •
A few are highly Pathogenic, •
Some commensals turn out to be pathogenic as in UTI after catheterization.
slide 3
Characters of Enterobacteriaceae •
All Enterobacteriaceae
a) – Gram-negative rods
b) – Ferment glucose with acid production
c) – Reduce nitrates into nitrites
d) – Oxidase negative
Facultative anaerobic
Motile except Shigella and Klebsiella
Non-capsulated except Klebsiella
Non-fastidious
Grow on bile containing media (MacConkey agar
slide7
Conserved signature inserts and deletions (CSIs) in protein sequences provide an important category of molecular markers for understanding phylogenetic relationships. ... The CSIs that are restricted to a particular clade or group of species, generally provide good phylogenetic markers of common evolutionary descent
slide 8
The family Enterobacteriaceae harbors many important pathogens, however it has proven difficult to reliably distinguish different members of this family or discern their interrelationships.
To understand the interrelationships among the Enterobacteriaceae species, we have constructed two comprehensive phylogenetic trees for 78 genome-sequenced Enterobacteriaceae species based on 2487 core genome proteins, and another set of 118 conserved proteins.
The genome sequences of Enterobacteriaceae species were also analyzed for genetic relatedness based on average amino acid identity and 16S rRNA sequence similarity.
In parallel, comparative genomic studies on protein sequences from the Enterobacteriaceae have identified 88 molecular markers in the form of conserved signature indels (CSIs) that are uniquely shared by specific members of the family.
slide 10 identification of enterobacteriaceae
biochemical reaction
Oxidase test
All members of Enterobacteriaceae are oxidase negative
Pseudomonas is oxidase positive
O/F test
All members of Enterobacteriaceae are O+/F+
Pseudomonas is O+/F-
Nitrate reductase
All members of Enterobacteriaceae are nitrate reductase positive
Pseudomonas is nitrate reductase negative
slide 11
identification of enterobacteriaceae
Differentiation between LF & NLF by Growth
Method:
on MacConkey agar
MacConkey agar is inoculated with tested organism using streak plate technique
Incubate the plate in incubator at 37 C/24 hrs
Results:
LF organism appears as pink colonies (e.g. E. coli)
NLF organism appears as colorless colonies (e.g. Shigella)
Dr. Matt Sturos - Senecavirus A Infection in BoarsJohn Blue
Senecavirus A Infection in Boars - Dr. Matt Sturos, from the 2017 Allen D. Leman Swine Conference, September 16-19, 2017, St. Paul, Minnesota, USA.
More presentations at http://www.swinecast.com/2017-leman-swine-conference-material
Dr. Matt Sturos - Impact of Senecavirus A in Neonate PigsJohn Blue
Impact of Senecavirus A in Neonate Pigs - Dr. Matt Sturos, from the 2018 Allen D. Leman Swine Conference, September 15-18, 2018, St. Paul, Minnesota, USA.
More presentations at http://www.swinecast.com/2018-leman-swine-conference-material
The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
I will finally argue that deep variability is both the problem and solution of frictionless reproducibility, calling the software science community to develop new methods and tools to manage variability and foster reproducibility in software systems.
Exposé invité Journées Nationales du GDR GPL 2024
ESR spectroscopy in liquid food and beverages.pptxPRIYANKA PATEL
With increasing population, people need to rely on packaged food stuffs. Packaging of food materials requires the preservation of food. There are various methods for the treatment of food to preserve them and irradiation treatment of food is one of them. It is the most common and the most harmless method for the food preservation as it does not alter the necessary micronutrients of food materials. Although irradiated food doesn’t cause any harm to the human health but still the quality assessment of food is required to provide consumers with necessary information about the food. ESR spectroscopy is the most sophisticated way to investigate the quality of the food and the free radicals induced during the processing of the food. ESR spin trapping technique is useful for the detection of highly unstable radicals in the food. The antioxidant capability of liquid food and beverages in mainly performed by spin trapping technique.
Nucleophilic Addition of carbonyl compounds.pptxSSR02
Nucleophilic addition is the most important reaction of carbonyls. Not just aldehydes and ketones, but also carboxylic acid derivatives in general.
Carbonyls undergo addition reactions with a large range of nucleophiles.
Comparing the relative basicity of the nucleophile and the product is extremely helpful in determining how reversible the addition reaction is. Reactions with Grignards and hydrides are irreversible. Reactions with weak bases like halides and carboxylates generally don’t happen.
Electronic effects (inductive effects, electron donation) have a large impact on reactivity.
Large groups adjacent to the carbonyl will slow the rate of reaction.
Neutral nucleophiles can also add to carbonyls, although their additions are generally slower and more reversible. Acid catalysis is sometimes employed to increase the rate of addition.
Travis Hills' Endeavors in Minnesota: Fostering Environmental and Economic Pr...Travis Hills MN
Travis Hills of Minnesota developed a method to convert waste into high-value dry fertilizer, significantly enriching soil quality. By providing farmers with a valuable resource derived from waste, Travis Hills helps enhance farm profitability while promoting environmental stewardship. Travis Hills' sustainable practices lead to cost savings and increased revenue for farmers by improving resource efficiency and reducing waste.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
New sequence variants of Staphylococcal Enterotoxins detected: host specificity and characterization study
1. New sequence variants of Staphylococcal
Enterotoxins detected: host specificity and
characterization study
Macori Guerrino1, Gallina Silvia1, Johler Sophia2, Sihto Henna-Maria2, Stephan Roger2 and Decastelli Lucia 1
1National Reference Laboratory for Coagulase-Positive Staphylococci including Staphylococcus aureus, Istituto Zooprofilattico Sperimentale
del Piemonte, Liguria e Valle d'Aosta, Torino, Italy
2Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Zurich, Switzerland.
28th- 30th September 2016
Pacengo di Lazise (VR)
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
Guerrino Macori, PhD
Post-Doctoral Researcher
Teagasc
APC - Microbiome Institute
guerrino.macori@teagasc.ie
@guerrinomacori
2. XVII Congresso Nazionale S.I.Di.LV.
Outline
1. Introduction
2. Aim of the study
3. Material and Methods
4. Results
5. Discussion
6. Conclusion
New Sequence Variants in S.aureus
NRL for
Coagulase Positive
Staphylococci including
Staphylococcus aureus
IZSPLV - Turin
3. Background
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
New Sequence Variants in S.aureus
4. Background
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
New Sequence Variants in S.aureus
5. Background
EFSA Journal 2015;13(12):4329
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
New Sequence Variants in S.aureus
6. 1. Introduction
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
Five major SEs
SEA, SEB, SEC, SED, SEE
New Sequence Variants in S.aureus
7. 1. Introduction
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
Strain-specific variation of SE mRNA and protein levels
environmental stress encountered in the food matrix
Glucose stress (30%) can lead to decreased sed expression in stationary phase.
•Enterotoxin D production is decreased in the presence of nitrite (c = 150 mg/L).
•Staphylococcus aureus growth is not significantly affected by nitrite (c = 150 mg/L).
New Sequence Variants in S.aureus
8. 1. Introduction
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
• Gene expression regulated by mRNA turnover modulation, factors in transcript
degradation are poorly understood.
• In S.aureus, the accessory regulator, SarA, (RNA-binding protein) turnover modulator.
• A sigma factor (σ factor) is a protein needed only for initiation of RNA synthesis.
• Alternative sigma factor B has an essential role in enterotoxin D regulation.
• Staphylococcal accessory regulator A positively regulates enterotoxin D production.
• Loss of accessory gene regulator does not alter enterotoxin D production.
New Sequence Variants in S.aureus
9. 1. Introduction
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
Accessory elements intro
Expression of the phage-encoded:
• life cycle of the phage
• nucleotide sequence variation in the sea gene and upstream promoter region
Ana Camacho, and Margarita Salas FEMS Microbiol Rev 2010;34:828-841
New Sequence Variants in S.aureus
SEA
10. 1. Introduction
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
Accessory elements and variation in SEB, SEC, and SED
• seb and sed promoters region from -98 to -59 is required for the expression and
regulation of seb
• region from -34 to +18 is required for sed promoter function
-98 -59
-34 +18
New Sequence Variants in S.aureus
SEB and SED
11. 1. Introduction
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
Accessory elements SEB
The seb gene resides in one of seven different S.aureus pathogenicity islands
Variation in SEB levels produced
Five allelic variants of SEB have been described that vary in biological activity
SEB SEB
SEB SEB
SEB SEB
New Sequence Variants in S.aureus
7 SaPIs
12. 1. Introduction
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
Accessory elements SEC
sec gene located in 4 different SaPIs.
• Four variants of SEC (SEC1-4) associated with human S.aureus strains
• Host-specific variants SEC-bovine and SEC-ovine
SaPIn1 SaPIm1
SaPImw2 SaPIbov1
SEC
New Sequence Variants in S.aureus
13. 1. Introduction
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
Accessory elements SED
• sed gene highly conserved, located on a plasmid
• strains harboring a single base deletion in various locations in the sed sequence
have been reported in S. aureus isolates from human.
Argudin et al., 2010)
New Sequence Variants in S.aureus
human hosts
14. 2. Aim of the study
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
• Analyse the promoter and gene
sequences of seb, sec, and sed
from S.aureus strains originating
from different sources;
• Strain-specific variation in SE
expression;
• Monitoring the evolution of
S.aureus pathogenicity and host
adaptation.
New Sequence Variants in S.aureus
BIOINFORMATIC CHALLENGE
15. 3. Materials and Methods
Bacterial Strains
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
350 isolates
DNA microarray enterotoxin hybridization patterns, spa types and clonal complexes .
Population studied (37 strains) published data and characteristics well described
• SFP outbreaks (9)
• symptomatic nasal colonization or cases of infections in humans (16)
• rabbit carcasses (8)
• bovine mastitis milk (4)
New Sequence Variants in S.aureus
12 SEB
10 SEC
15 SED
16. 3. Materials and Methods
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
• 350 strains DNA Extraction and PCR Amplification
• Microarray and molecular typing
• Selected strains PCR Purification and Sequencing
• Sequence variants: Toxin detection by SET-RPLA
• Amino acid identity, bioinformatic
New Sequence Variants in S.aureus
17. 4. Results
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
seb Promoter and Gene Sequences (12 strains)
Five variants of the seb promoter (sevp v1–v5)
New Sequence Variants in S.aureus
18. 4. Results
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
New Sequence Variants in S.aureus
V1 Multiple positions: Four variants of the seb gene (seb v1–v4) Firstly described
19. 4. Results
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
New Sequence Variants in S.aureus
V2 Multiple positions: Four variants of the seb gene (seb v1–v4) Firstly described
20. 4. Results
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
New Sequence Variants in S.aureus
V3 Multiple positions: Four variants of the seb gene (seb v1–v4) Firstly described
21. 4. Results
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
New Sequence Variants in S.aureus
V4 Multiple positions: Four variants of the seb gene (seb v1–v4) Firstly described
22. 4. Results
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
Three variants of the sec promoter (secp v1–v3)
Four variants of the sec gene (sec v1–v4)
sec Promoter and Gene Sequences (10 strains)
New Sequence Variants in S.aureus
23. 4. Results
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
New Sequence Variants in S.aureus
24. 4. Results
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
Three variants of the sed promoter (sedp v1–v3)
New Sequence Variants in S.aureus
Sed Promoter and Gene Sequences (12 strains)
25. 4. Results
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
New Sequence Variants in S.aureus
Sed Promoter and Gene Sequences (12 strains)
Four variants of the sed gene (sed v1–v4)
26. 4. Results
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
New Sequence Variants in S.aureus
Four variants of the sed Amino Avid (sed v1–v4)
Rabbit
carcasses
27. 4. Results
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
New Sequence Variants in S.aureus
Four variants of the sed Amino Acid (sed v1–v4)
Rabbit carcasses
STOP CODON
28. 4. Results
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
XVII Congresso Nazionale S.I.Di.LV.
New Sequence Variants in S.aureus
Four variants of the sed Amino Acid (sed v1–v4)
Rabbit carcasses
STOP CODON
POSITIVE RESULTS – ENTEROTOXIN DETECTED
SET-RPLA
29. One SED variant exhibited a deletion in sed
premature stop codon and a truncated sed amino acid precursor.
FBOs isolates, deletions at two nucleotide positions (150 and 514)
premature stop codon
A deletion in sed identical to the one seen in rabbit isolates in this study (nucleotide position
521) has been reported in S. aureus isolates originating from humans and from food but not
success the detection by Western Blot and qPCR.
In contrast, in this study, three of four rabbit strains tested with truncated sed variants yielded
a weak, but positive result for SED in the SET-RPLA assay
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
5. Discussion
New Sequence Variants in S.aureus
XVII Congresso Nazionale S.I.Di.LV.
30. 6. Conclusion
1. sequence variation in enterotoxin genes of S. aureus strains isolated from various sources.
2. All SED+ rabbit strains tested in this study harbored a sed variant that exhibited a deletion in sed
leading to a premature stop codon.
3. The sequence data -> sequence variation in enterotoxin genes of S. aureus strains isolated from
various sources, truncated proteins and specificity of host?
Prospects: quantifying gene expression?
mRNA, genomic profiles, Mass spectrometry?
NRL for
Coagulase Positive Staphylococci
including Staphylococcus aureus
New Sequence Variants in S.aureus
XVII Congresso Nazionale S.I.Di.LV.
31. • Thanks to SIDiLV for the opportunity;
• Thanks to the Institute for Food Safety and Hygiene (IFSH) of the University of Zurich and the Director Stephan Roger for guest me
as a Visitor Scientist and the
• Thanks to the researcher of the IFSH, Sophia Johler for the great experience, the competence and the quality of the researches she
is conducting. It was very inspiring , I learnt a lot!
• Thanks to Henna-Maria Sihto for the collaboration;
• Thanks to the Italian NRL for Coagulase Positive Staphylococci, in particular the Head of the Laboratory Lucia Decastelli and Silvia
Gallina for the chance to work with you!
• The researchers of the Food Control lab in Turin;
• Thanks to Paul D Cotter the head of the Vision I lab at Teagasc, Food Bioscience Dept. (APC Microbiome Institute Fermoy, Co.
Cork, Ireland) where I’m working.
Guerrino Macori, PhD
Post-Doctoral Researcher
Teagasc
APC - Microbiome Institute
guerrino.macori@teagasc.ie
@guerrinomacori
Keep in touch!