neoplex pcr test kit [genematrix advantages of neo_plex covid-19 Rapid Test K...HK HuZef
This Presentation Briefly Explains All About Neoplex Pcr Test Kit, for Corona Virus Detection. Pcr Covid-19 Rapid test Kits Have Advantages Over Other Manufacturers.
Pcr technology and its importance in covid 19 pandemicAnupam Maity
Since the discovery of the PCR technology, its application in the various fields is increased gradually. Based on to this principle, many variations of the PCR have been established. Year by year, it is upgraded very much. It is established as a most common and accurate technique for the detection of the various diseases in the field of medicine. Now it is a ‘Gold standard’ for the detection of covid-19 also, which is much needed to contain the spread of the virus. Though various detection techniques are there for detection, but real time RT-PCR (variation of PCR) is most reliable. Viral detection is based on a simple principle of nucleic acid (viral) amplification. Various manufacturing companies are manufacturing the PCR instrument. Though the accuracy of the instruments are slightly differ to each other.
Polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail. PCR was invented in 1984 by the American biochemist Kary Mullis at Cetus Corporation. It is fundamental to much of genetic testing including analysis of ancient samples of DNA and identification of infectious agents. Using PCR, copies of very small amounts of DNA sequences are exponentially amplified in a series of cycles of temperature changes. PCR is now a common and often indispensable technique used in medical laboratory and clinical laboratory research for a broad variety of applications including biomedical research and criminal forensics
Cancer Research & the Challenges of FFPE Samples – An IntroductionQIAGEN
A cascade of complex genetic and epigenetic changes regulate tumor formation and progression. Gene expression analyses can shed light on these changes at a molecular level and identify the key genes and associated pathways involved in cancer. Often the samples used in cancer research are FFPE samples, which pose a significant challenge in terms of nucleic acid quality. The quality of nucleic acids extracted from FFPE samples depends on a number of factors, including how the samples were handled before, during and after fixation and embedding.
Dr. Vishwadeepak Tripathi describes the variability of sample purification from FFPE samples – in particular, samples to be used in cancer research. What are the challenges and solutions, and what quality control approach can ensure credible results? This webinar will focus on sample purification and the quality control of FFPE samples and compare different automated purification procedures.
RotorGene Q A Rapid, Automatable real-time PCR Instrument for Genotyping and...QIAGEN
QIAGEN has developed a selection of robust, novel chemistries to prevent PCR crosstalk. We can successfully measure target abundance and fold change in real-time assays, and perform sub-genotyping using a fast, high-throughput and powerful High-Resolution Melting (HRM) statistical analysis program. In this presentation, we will demonstrate these features and benefits with examples.
Automated Nucleic Acid Purification from Diverse Sample types using dedicated...QIAGEN
This webinar will focus on the automation of QIAGEN’s new line of DNA and RNA sample prep kits for the microbiome. We will show how automation on the QIAcube enables efficient and reliable use of these samples for sensitive downstream applications such as qPCR and NGS. In addition, you will learn how to successfully use the CLC Microbial Genomics Module for metagenome sequencing and identification of microbial composition and diversity.
neoplex pcr test kit [genematrix advantages of neo_plex covid-19 Rapid Test K...HK HuZef
This Presentation Briefly Explains All About Neoplex Pcr Test Kit, for Corona Virus Detection. Pcr Covid-19 Rapid test Kits Have Advantages Over Other Manufacturers.
Pcr technology and its importance in covid 19 pandemicAnupam Maity
Since the discovery of the PCR technology, its application in the various fields is increased gradually. Based on to this principle, many variations of the PCR have been established. Year by year, it is upgraded very much. It is established as a most common and accurate technique for the detection of the various diseases in the field of medicine. Now it is a ‘Gold standard’ for the detection of covid-19 also, which is much needed to contain the spread of the virus. Though various detection techniques are there for detection, but real time RT-PCR (variation of PCR) is most reliable. Viral detection is based on a simple principle of nucleic acid (viral) amplification. Various manufacturing companies are manufacturing the PCR instrument. Though the accuracy of the instruments are slightly differ to each other.
Polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail. PCR was invented in 1984 by the American biochemist Kary Mullis at Cetus Corporation. It is fundamental to much of genetic testing including analysis of ancient samples of DNA and identification of infectious agents. Using PCR, copies of very small amounts of DNA sequences are exponentially amplified in a series of cycles of temperature changes. PCR is now a common and often indispensable technique used in medical laboratory and clinical laboratory research for a broad variety of applications including biomedical research and criminal forensics
Cancer Research & the Challenges of FFPE Samples – An IntroductionQIAGEN
A cascade of complex genetic and epigenetic changes regulate tumor formation and progression. Gene expression analyses can shed light on these changes at a molecular level and identify the key genes and associated pathways involved in cancer. Often the samples used in cancer research are FFPE samples, which pose a significant challenge in terms of nucleic acid quality. The quality of nucleic acids extracted from FFPE samples depends on a number of factors, including how the samples were handled before, during and after fixation and embedding.
Dr. Vishwadeepak Tripathi describes the variability of sample purification from FFPE samples – in particular, samples to be used in cancer research. What are the challenges and solutions, and what quality control approach can ensure credible results? This webinar will focus on sample purification and the quality control of FFPE samples and compare different automated purification procedures.
RotorGene Q A Rapid, Automatable real-time PCR Instrument for Genotyping and...QIAGEN
QIAGEN has developed a selection of robust, novel chemistries to prevent PCR crosstalk. We can successfully measure target abundance and fold change in real-time assays, and perform sub-genotyping using a fast, high-throughput and powerful High-Resolution Melting (HRM) statistical analysis program. In this presentation, we will demonstrate these features and benefits with examples.
Automated Nucleic Acid Purification from Diverse Sample types using dedicated...QIAGEN
This webinar will focus on the automation of QIAGEN’s new line of DNA and RNA sample prep kits for the microbiome. We will show how automation on the QIAcube enables efficient and reliable use of these samples for sensitive downstream applications such as qPCR and NGS. In addition, you will learn how to successfully use the CLC Microbial Genomics Module for metagenome sequencing and identification of microbial composition and diversity.
Sequential Automation of RNA and DNA preps on the same QIAcube instrumentQIAGEN
Automation of QIAGEN spin-column kits on the QIAcube saves valuable time and ensures standardized results. Since the same QIAcube may be used by multiple researchers for different applications, cross-contamination between samples and preparation technologies must be avoided (e.g., when nucleases are used). The unique instrument design and features minimize contamination between sequential preps, allowing both RNA and DNA preps to be performed on the same instrument. To show the process safety and robustness, we performed alternating automated RNA preps (requiring a DNase step) and DNA plasmid preps (requiring an RNase step). The preps were sequentially performed on the same QIAcube instrument using the RNeasy® Mini Kit and the QIAprep® Spin Miniprep Kit, respectively.
Independently, we performed a series of manually processed preps to compare with the automated preps. RNA and DNA quality and yields were similar between the two methods, showing the absence of carryover of nucleases.
Corona is here to stay and it is predicted that over 70% of population will get the infection (fortunately not all will fall sick or very sick). (Recovery rate of over 74% & Death rate around 2%).
A lot of confusion exists regarding testing for covid and what test to do, when and how to interpret these tests.
Compiled by Dr. Narendra Malhotra
Stable 16 year storage of DNA purified with the QIAamp® DNA Blood mini kit - ...QIAGEN
In this application note, we describe the success of the QIAamp DNA Blood Mini Kit in the preparation of highly stable DNA,as evidenced by 16-year storage data. We also report the best storage conditions for maximal protection against degradation.
2011 course on Molecular Diagnostic Automation - Part 2 - AmplificationPatrick Merel
2011 course on Molecular Diagnostic Automation - Part 2 - Amplification.
This is from early 2011. Prices and Specifications of instruments may have changed.
Part 2 of 3
Optimal RNAlater® incubation and removal conditions prior to isolation of tot...QIAGEN
RNA is highly sensitive to degradation. Handling methods and prolonged storage of cells can greatly affect the quality of the RNA that can be later isolated. Contamination with RNases is the most significant problem, especially as they are so ubiquitous in the environment. They can degrade RNA to the point where results of downstream analyses become meaningless.
Submerging cells in RNAlater, an RNA stabilization reagent, helps to stabilize the RNA within the cells and prevent degradation, supporting accurate downstream gene expression analyses. However, to avoid any interference from any RNAlater components in isolation and analyses, cells must be pelleted and the reagent must be removed. The separation of cells from excess RNAlater via centrifugation is impeded due to the higher density of the reagent compared to standard culture medium. This means it requires higher centrifugal forces, which might damage cells due to increased shearing forces, leading to reduced RNA yield. The aim of this study was to establish the optimal conditions for the recovery of cells from RNAlater after RNA stabilization for maximum RNA yield and integrity.
SARS-CoV-2 (COVID-19) is the virus responsible for respiratory disease caused by a novel (new) coronavirus that was first detected in Wuhan City, Hubei Province, China, later causing a global pandemic.
The virus is contagious and can be spread from humans to humans primarily through the exchange of mucus droplets that are expelled through sneezes or coughs.
The SARS-CoV-2 is a severe acute respiratory syndrome coronavirus. This outbreak is an important reminder that the global community must strengthen national and international programs for detection and response to future disease outbreaks.
Automated DNA purification from diverse Microbiome samples using dedicated Mi...QIAGEN
This application note demonstrates the automation of QIAGEN’s new line of DNA sample prep kits for the microbiome. The microbiome of samples as diverse as soil, water and stool was purified using dedicated QIAcube compatible kits. Automation on the QIAcube enabled efficient and reliable use of these samples for sensitive downstream applications such as qPCR and NGS. In addition, the CLC Microbial Genomics Module was successfully employed for metagenome sequencing and identification of microbial composition and diversity.
Simultaneous Isolation of RNA & DNA from one FFPE SampleQIAGEN
Worldwide, there are millions of tissue samples archived in tissue biobanks and biorepositories. These samples are extremely valuable for pharmacological and biomedical research and companion diagnostics, due to the linkage to patient history. The vast majority of archived tissue samples are formalin-fixed and paraffin-embedded (FFPE), since formalin is the standard fixative for tissue samples.
FFPE blocks serve as an excellent source for histomorphology studies, but their use in molecular studies is challenging, due to crosslinking and fragmentation caused by fixation, processing, embedding, and storage conditions. For reliable comparison of genomic and transcriptomic data from heterogeneous samples and to spare sample material, purification of DNA and RNA from
the same sample is essential. This is particularly important when working with tumorous tissues, which contain a heterogeneous distribution of healthy and malignant cells.
Critical Steps for Real-Time PCR Analysis: Tips and Solutions to Achieve Effi...QIAGEN
In this slidedeck, we cover the following topics which are critical steps for efficient and precise gene expression studies using real-time PCR technology:
1) Effect of RNA integrity on real-time PCR results – tips to achieve a true RNA profiling suitable for real-time PCR studies
2) Improved methods for cDNA synthesis, optimized for real-time PCR
3) Real-time PCR analysis:
• Real-time PCR essentials and background information on different quantification strategies
• SYBR Green real-time PCR – factors influencing specificity
• Introduction to probe technology
• New, fast and efficient real-time PCR solutions
Maximizing PCR and RT-PCR Success - Download the BrochureQIAGEN
The invention of the polymerase chain reaction (PCR) by K. Mullis and coworkers in 1985 revolutionized molecular biology and molecular medicine. Major research areas, such as biomarker discovery, gene regulation and cancer research are challenging today’s PCR technologies with more demanding requirements. These include the need for increased throughput while reducing costs, higher assay sensitivity and reliable data normalization. Assay development and evaluation, reproducibility of data and time to result are still major problems encountered by researchers.
Meeting today’s challenges in PCR requires advances in all methods of the workflow that starts with sample collection, sample stabilization, and nucleic acid purification, and ends with amplification and detection. The following pages focus on the importance of amplification in meeting these challenges.
RNA Integrity and Quality – Standardize RNA Quality Control QIAGEN
RNA integrity and quality are critical to obtain meaningful and reliable downstream data. This slidedeck details the challenges and considerations of handling RNA samples, the need for quality control analysis and common methods for RNA integrity and quality assessment. The QIAxcel Advanced System will be introduced to automate the process of RNA sample integrity analysis and obtain objective quality measurement. Application data will be presented.
Advancing Microbiome Research: From challenging samples to insight with Confi...QIAGEN
Microbiome research encompasses sample types as diverse as the human gut, Antarctic soil, ocean water and acidic hot spring biofilms. These samples are challenging because they are difficult to lyse, with some microbes containing a tough extracellular matrix. Incomplete lysis of a microbial community results in an inaccurate representation of the microbial content of the sample. Additionally, PCR inhibitors present in these samples, especially humic acids, polysaccharides, polyphenolics, lipids and heavy metals result in inaccurate quantification of nucleic acids that may inhibit downstream applications such as qPCR and NGS.
QIAcube® RNA isolation from stool samples using the RNeasy® PowerMicrobiome® ...QIAGEN
This application note demonstrates that RNA is extracted efficiently from stool samples using the RNeasy PowerMicrobiome Kit and the QIAcube system. Furthermore, the RNA isolated with the RNeasy PowerMicrobiome Kit and the QIAcube system is compatible with downstream applications.
Automated DNA extraction from FFPE tissue using a xylene free deparaffinizati...QIAGEN
Formalin-fixed paraffin-embedded (FFPE) tissue samples are routinely used for immunohistochemistry and molecular analysis in cancer research. However, many methods for DNA extraction from FFPE tissue sections are manual procedures that are not standardized, time consuming and often involve the use of hazardous materials like xylene. Recently we introduced an automated solution for the DNA extraction from FFPE tissue using the QIAsymphony SP instrument in combination with the QIAsymphony DNA Mini kit.
Practical hints and new solutions for successful real-time PCR studies QIAGEN
Part 1: Practical hints and new solutions for successful real-time PCR studies
In this webinar we will cover the following topics which are critical steps for efficient and precise gene expression studies using real-time PCR technology:
- Effect of RNA integrity on real-time PCR results – tips to achieve a true RNA profiling suitable for real-time PCR studies
- Improved methods for cDNA synthesis, optimized for real-time PCR
- Real-time PCR analysis
o Real-time PCR essentials and background information on different quantification strategies
o SYBR Green real-time PCR – factors influencing specificity
o Introduction to probe technology
o New, fast and efficient real-time PCR solutions
Part 2: Critical Factors for Successful Multiplex Real-Time PCR
Multiplex real-time PCR is a powerful tool for gene expression analysis, viral load monitoring, genotyping, and many other applications. The ability to amplify and detect several genomic DNA, cDNA, or RNA targets in the same reaction offers many benefits:
• Conservation of precious samples – more quantification data per sample
• Increased throughput – more targets analyzed per run on a cycler
• Reliable results – no well-to-well variability due to co-amplification of internal control
• Reduced costs – save time and reagents
The QuantiFast Multiplex PCR and RT-PCR kits are optimized for reliable amplification of many different templates despite a high variability in abundance. Thus they enable successful amplification of multiple targets on the first attempt without optimization.
This webinar explains the principles of the QIAGEN multiplex technologies and shows data demonstrating the exceptional multiplex real-time PCR performance of the QuantiFast Multiplex kits.
Rapid DNA isolation from diverse plant material for use in Next Generation Se...QIAGEN
Isolation of DNA from plant material is often a tedious process which involves significant hands on time and leads to varying results due to the diverse nature of the material. Different parts of the plants as well as the plants themselves differ in both consistency of material and presence of inhibitory substances, making dependable isolation of DNA difficult.
Here, we developed a method for the efficient extraction of DNA from different plant types, including strawberry leaf, pine needle, grape leaf, and cotton and coffee seeds (workflow at right). A novel bead beating method and lysis chemistry led to more efficient sample lysis with minimal hands-on time and significantly increased DNA yield compared to conventional methods. Through the use of multiple technologies to improve removal of secondary metabolites, such as polyphenols, complex polysaccharides, alkaloids and tannins that may inhibit downstream applications, the isolated DNA was of high quality and purity.
The resulting DNA is suitable for immediate use in downstream reactions, including PCR, qPCR and Next Generation Sequencing based applications. Using this method we were further able to design a workflow that included DNA isolation, library preparation and bioinformatics analyses for the efficient detection of plant pathogens isolated from infected samples. With this, our protocol is a substantial improvement within workflows used for plant microbiome and plant pathology studies as well as in plant breeding and engineering.
Sequential Automation of RNA and DNA preps on the same QIAcube instrumentQIAGEN
Automation of QIAGEN spin-column kits on the QIAcube saves valuable time and ensures standardized results. Since the same QIAcube may be used by multiple researchers for different applications, cross-contamination between samples and preparation technologies must be avoided (e.g., when nucleases are used). The unique instrument design and features minimize contamination between sequential preps, allowing both RNA and DNA preps to be performed on the same instrument. To show the process safety and robustness, we performed alternating automated RNA preps (requiring a DNase step) and DNA plasmid preps (requiring an RNase step). The preps were sequentially performed on the same QIAcube instrument using the RNeasy® Mini Kit and the QIAprep® Spin Miniprep Kit, respectively.
Independently, we performed a series of manually processed preps to compare with the automated preps. RNA and DNA quality and yields were similar between the two methods, showing the absence of carryover of nucleases.
Corona is here to stay and it is predicted that over 70% of population will get the infection (fortunately not all will fall sick or very sick). (Recovery rate of over 74% & Death rate around 2%).
A lot of confusion exists regarding testing for covid and what test to do, when and how to interpret these tests.
Compiled by Dr. Narendra Malhotra
Stable 16 year storage of DNA purified with the QIAamp® DNA Blood mini kit - ...QIAGEN
In this application note, we describe the success of the QIAamp DNA Blood Mini Kit in the preparation of highly stable DNA,as evidenced by 16-year storage data. We also report the best storage conditions for maximal protection against degradation.
2011 course on Molecular Diagnostic Automation - Part 2 - AmplificationPatrick Merel
2011 course on Molecular Diagnostic Automation - Part 2 - Amplification.
This is from early 2011. Prices and Specifications of instruments may have changed.
Part 2 of 3
Optimal RNAlater® incubation and removal conditions prior to isolation of tot...QIAGEN
RNA is highly sensitive to degradation. Handling methods and prolonged storage of cells can greatly affect the quality of the RNA that can be later isolated. Contamination with RNases is the most significant problem, especially as they are so ubiquitous in the environment. They can degrade RNA to the point where results of downstream analyses become meaningless.
Submerging cells in RNAlater, an RNA stabilization reagent, helps to stabilize the RNA within the cells and prevent degradation, supporting accurate downstream gene expression analyses. However, to avoid any interference from any RNAlater components in isolation and analyses, cells must be pelleted and the reagent must be removed. The separation of cells from excess RNAlater via centrifugation is impeded due to the higher density of the reagent compared to standard culture medium. This means it requires higher centrifugal forces, which might damage cells due to increased shearing forces, leading to reduced RNA yield. The aim of this study was to establish the optimal conditions for the recovery of cells from RNAlater after RNA stabilization for maximum RNA yield and integrity.
SARS-CoV-2 (COVID-19) is the virus responsible for respiratory disease caused by a novel (new) coronavirus that was first detected in Wuhan City, Hubei Province, China, later causing a global pandemic.
The virus is contagious and can be spread from humans to humans primarily through the exchange of mucus droplets that are expelled through sneezes or coughs.
The SARS-CoV-2 is a severe acute respiratory syndrome coronavirus. This outbreak is an important reminder that the global community must strengthen national and international programs for detection and response to future disease outbreaks.
Automated DNA purification from diverse Microbiome samples using dedicated Mi...QIAGEN
This application note demonstrates the automation of QIAGEN’s new line of DNA sample prep kits for the microbiome. The microbiome of samples as diverse as soil, water and stool was purified using dedicated QIAcube compatible kits. Automation on the QIAcube enabled efficient and reliable use of these samples for sensitive downstream applications such as qPCR and NGS. In addition, the CLC Microbial Genomics Module was successfully employed for metagenome sequencing and identification of microbial composition and diversity.
Simultaneous Isolation of RNA & DNA from one FFPE SampleQIAGEN
Worldwide, there are millions of tissue samples archived in tissue biobanks and biorepositories. These samples are extremely valuable for pharmacological and biomedical research and companion diagnostics, due to the linkage to patient history. The vast majority of archived tissue samples are formalin-fixed and paraffin-embedded (FFPE), since formalin is the standard fixative for tissue samples.
FFPE blocks serve as an excellent source for histomorphology studies, but their use in molecular studies is challenging, due to crosslinking and fragmentation caused by fixation, processing, embedding, and storage conditions. For reliable comparison of genomic and transcriptomic data from heterogeneous samples and to spare sample material, purification of DNA and RNA from
the same sample is essential. This is particularly important when working with tumorous tissues, which contain a heterogeneous distribution of healthy and malignant cells.
Critical Steps for Real-Time PCR Analysis: Tips and Solutions to Achieve Effi...QIAGEN
In this slidedeck, we cover the following topics which are critical steps for efficient and precise gene expression studies using real-time PCR technology:
1) Effect of RNA integrity on real-time PCR results – tips to achieve a true RNA profiling suitable for real-time PCR studies
2) Improved methods for cDNA synthesis, optimized for real-time PCR
3) Real-time PCR analysis:
• Real-time PCR essentials and background information on different quantification strategies
• SYBR Green real-time PCR – factors influencing specificity
• Introduction to probe technology
• New, fast and efficient real-time PCR solutions
Maximizing PCR and RT-PCR Success - Download the BrochureQIAGEN
The invention of the polymerase chain reaction (PCR) by K. Mullis and coworkers in 1985 revolutionized molecular biology and molecular medicine. Major research areas, such as biomarker discovery, gene regulation and cancer research are challenging today’s PCR technologies with more demanding requirements. These include the need for increased throughput while reducing costs, higher assay sensitivity and reliable data normalization. Assay development and evaluation, reproducibility of data and time to result are still major problems encountered by researchers.
Meeting today’s challenges in PCR requires advances in all methods of the workflow that starts with sample collection, sample stabilization, and nucleic acid purification, and ends with amplification and detection. The following pages focus on the importance of amplification in meeting these challenges.
RNA Integrity and Quality – Standardize RNA Quality Control QIAGEN
RNA integrity and quality are critical to obtain meaningful and reliable downstream data. This slidedeck details the challenges and considerations of handling RNA samples, the need for quality control analysis and common methods for RNA integrity and quality assessment. The QIAxcel Advanced System will be introduced to automate the process of RNA sample integrity analysis and obtain objective quality measurement. Application data will be presented.
Advancing Microbiome Research: From challenging samples to insight with Confi...QIAGEN
Microbiome research encompasses sample types as diverse as the human gut, Antarctic soil, ocean water and acidic hot spring biofilms. These samples are challenging because they are difficult to lyse, with some microbes containing a tough extracellular matrix. Incomplete lysis of a microbial community results in an inaccurate representation of the microbial content of the sample. Additionally, PCR inhibitors present in these samples, especially humic acids, polysaccharides, polyphenolics, lipids and heavy metals result in inaccurate quantification of nucleic acids that may inhibit downstream applications such as qPCR and NGS.
QIAcube® RNA isolation from stool samples using the RNeasy® PowerMicrobiome® ...QIAGEN
This application note demonstrates that RNA is extracted efficiently from stool samples using the RNeasy PowerMicrobiome Kit and the QIAcube system. Furthermore, the RNA isolated with the RNeasy PowerMicrobiome Kit and the QIAcube system is compatible with downstream applications.
Automated DNA extraction from FFPE tissue using a xylene free deparaffinizati...QIAGEN
Formalin-fixed paraffin-embedded (FFPE) tissue samples are routinely used for immunohistochemistry and molecular analysis in cancer research. However, many methods for DNA extraction from FFPE tissue sections are manual procedures that are not standardized, time consuming and often involve the use of hazardous materials like xylene. Recently we introduced an automated solution for the DNA extraction from FFPE tissue using the QIAsymphony SP instrument in combination with the QIAsymphony DNA Mini kit.
Practical hints and new solutions for successful real-time PCR studies QIAGEN
Part 1: Practical hints and new solutions for successful real-time PCR studies
In this webinar we will cover the following topics which are critical steps for efficient and precise gene expression studies using real-time PCR technology:
- Effect of RNA integrity on real-time PCR results – tips to achieve a true RNA profiling suitable for real-time PCR studies
- Improved methods for cDNA synthesis, optimized for real-time PCR
- Real-time PCR analysis
o Real-time PCR essentials and background information on different quantification strategies
o SYBR Green real-time PCR – factors influencing specificity
o Introduction to probe technology
o New, fast and efficient real-time PCR solutions
Part 2: Critical Factors for Successful Multiplex Real-Time PCR
Multiplex real-time PCR is a powerful tool for gene expression analysis, viral load monitoring, genotyping, and many other applications. The ability to amplify and detect several genomic DNA, cDNA, or RNA targets in the same reaction offers many benefits:
• Conservation of precious samples – more quantification data per sample
• Increased throughput – more targets analyzed per run on a cycler
• Reliable results – no well-to-well variability due to co-amplification of internal control
• Reduced costs – save time and reagents
The QuantiFast Multiplex PCR and RT-PCR kits are optimized for reliable amplification of many different templates despite a high variability in abundance. Thus they enable successful amplification of multiple targets on the first attempt without optimization.
This webinar explains the principles of the QIAGEN multiplex technologies and shows data demonstrating the exceptional multiplex real-time PCR performance of the QuantiFast Multiplex kits.
Rapid DNA isolation from diverse plant material for use in Next Generation Se...QIAGEN
Isolation of DNA from plant material is often a tedious process which involves significant hands on time and leads to varying results due to the diverse nature of the material. Different parts of the plants as well as the plants themselves differ in both consistency of material and presence of inhibitory substances, making dependable isolation of DNA difficult.
Here, we developed a method for the efficient extraction of DNA from different plant types, including strawberry leaf, pine needle, grape leaf, and cotton and coffee seeds (workflow at right). A novel bead beating method and lysis chemistry led to more efficient sample lysis with minimal hands-on time and significantly increased DNA yield compared to conventional methods. Through the use of multiple technologies to improve removal of secondary metabolites, such as polyphenols, complex polysaccharides, alkaloids and tannins that may inhibit downstream applications, the isolated DNA was of high quality and purity.
The resulting DNA is suitable for immediate use in downstream reactions, including PCR, qPCR and Next Generation Sequencing based applications. Using this method we were further able to design a workflow that included DNA isolation, library preparation and bioinformatics analyses for the efficient detection of plant pathogens isolated from infected samples. With this, our protocol is a substantial improvement within workflows used for plant microbiome and plant pathology studies as well as in plant breeding and engineering.
Validation of anti niv igm capture elisa version#1krishgen
NiV is a negative-sense, non-segmented RNA virus that was first isolated from cerebrospinal fluid of human patients and classified in the family Paramyxoviridae under the new genus
Henipavirus. Its genome encodes six structural proteins: the nucleocapsid (N) protein,
phosphoprotein (P), matrix (M) protein, fusion (F) protein, glycoprotein (G), and large (L)
protein.
Nipah virus glycoprotein G has a globular head domain formed of a six-bladed beta sheet propeller, connected via a flexible stalk domain to a transmembrane anchor. The G binds to the cellular receptors ephrin B2 are ephrin B3, mediating viral attachment. Following attachment Nipah Virus glycoprotein G undergoes a conformational change that leads to triggering of glycoprotein F which leads to membrane fusion (Biering et al, 2012).
The Nipah virus glycoprotein G is a recombinant protein expressed in mammalian HEK293 cells. It is presented as a fusion protein with a mouse Fc tag linked to the C-terminus of glycoprotein G, amino acids 71-602.
We established preliminary specifications defining acceptable ranges for the parameters indicated herein below for our Anti Nipah Virus IgM Capture ELISA kit. These parameters were tracked day-to-day, run-to-run, and operator-to-operator, over a schedule defined inhouse.
Recommended assay characteristics included absorbance of a zero concentration standard; factors which describe the calibration for each standard and statistical description of the calibration curve such as coefficient of correlation, slope and/or intercept; and recovery of results on control samples. It is important to be able to relate the specifications for a parameter to expected reliability of the result. Our in-house standard defined was r=0.990.
User Menual How To USE Covid-19 Antibody Tests By Healgen HK HuZef
The COVID-19 IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma) is
a lateral flow immunoassay intended for the qualitative detection and differentiation
of IgM and IgG antibodies to SARS-CoV-2 in human venous whole blood, plasma
from anticoagulated blood (Li+ heparin, K2EDTA and sodium citrate), or
serum. The COVID-19 IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma)
is intended for use as an aid in identifying individuals with an adaptive immune
response to SARS-CoV-2, indicating recent or prior infection. At this time, it is
unknown for how long antibodies persist following infection and if the presence of
antibodies confers protective immunity. The COVID-19 IgG/IgM Rapid Test
Cassette (Whole Blood/Serum/Plasma) should not be used to diagnose acute
SARS-CoV-2 infection. Testing is limited to laboratories certified under the Clinical
Laboratory Improvement Amendments of 1988 (CLIA), 42 U.S.C 263a, to perform
moderate or high complexity tests.
Results are for the detection of SARS CoV-2 antibodies. IgM and IgG antibodies to
SARS-CoV-2 are generally detectable in blood several days after initial infection,
although the duration of time antibodies are present post-infection is not well
characterized. Individuals may have detectable virus present for several weeks
following seroconversion.
Laboratories within the United States and its territories are required to report all
positive results to the appropriate public health authorities.
The sensitivity of COVID-19 IgG/IgM Rapid Test Cassette (Whole
Blood/Serum/Plasma) early after infection in unknown. Negative results do not
preclude acute SARS-CoV-2 infection. If acute infection is suspected, direct testing
for SARS-CoV-2 is necessary.
False positive results for COVID-19 IgG/IgM Rapid Test Cassette (Whole
Blood/Serum/Plasma) may occur due to cross-reactivity from pre-existing
antibodies or other possible causes. Due to the risk of false positive results,
confirmation of positive results should be considered using second, different IgG or
IgM assay.
The COVID-19 IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma) is only
for use under the Food and Drug Administration’s Emergency Use Authorization.
Foregene's detection solution to covid 19Maggie Ma
In response to the Covid-19, Foregene developes the RT-PCR kit within 3 days.Based on Direct PCR tech, test centers needn't buy extra nucleic acid extraction kit and machine, just do PCR directly.It's so economical way. This kit is CE certificated, and welcomed by 10+ countries with stable quality and competitive prices.
The variant nucleic acid detection kit for Brazil, UK,India,and South Africa is also available with high specificity and sensitivity.
Are you a medical device importer?
Welcome your enquiry. E-mail:maggie@foregene.com
In this slide contains introduction, genomic materials of virus and testing method of covid 19 by using RT-PCR.
Presented by: R.Rekha (Department of pharmacology),
RIPER, anantapur.
The interfering effects of biotin concentrations ranging between 625 ng/mL and 10 µg/mL were
tested in a separate study. Biotin concentrations up to 1.25 µg/ml did not lead to false results.
Biotin concentrations ≥2.5 µg/ml can cause false-negative COVID-19 results with the
CareStart™ COVID-19 Antigen.
High-dose Hook Effect
The CareStart™ COVID-19 Antigen was tested up to 105 TCID50/ml of heat-inactivated SARSCoV-2 strain and no high-dose hook effect was observed.
Point of Care Use
The CareStart™ COVID-19 Antigen was demonstrated at near patient or Point of Care (POC)
testing that non-laboratory personnel can perform the test accurately in the intended use
environment. In addition, the robust use of the CareStart™ COVID-19 Antigen for near patient
or Point of Care (POC) testing was demonstrated by thirteen (13) Flex studies.
Technical Support
For questions, or to report a problem, please call Technical Support at +1-888-898-1270
(Available Hours: Mon. to Fri.: 8 a.m. – 5 p.m.) or TShelp@accessbio.net (24/7 available).
Test system problems may also be reported to the FDA using the MedWatch reporting system
(phone: 1-800 FDA-1088; fax: 1-800 FDA-1078: or http://www.fda.gov/medwatch)
For Emergency Use Authorization (EUA) Only
The CareStartTM COVID-19 Antigen test is a lateral ow immunochromatographic assay intended for the qualitative detection of the nucleocapsid protein antigen from SARS-CoV-2
in nasopharyngeal swab specimens directly collected from individuals who are suspected of COVID-19 by their healthcare provider within ve days of symptom onset.
IMPORTANT!
- Refer to the Package Insert for Warnings and Precautions, Specimen Collection Procedures, Storage and Handling Conditions, and Quality Control Recommendations.
- Warning and Precautions - All kit components can be discarded as Biohazard waste according to local guidelines. Refer to the product safety data sheet for risk and safety phrases and disposal information.
- Biotin Interference: False negative results may occur in patients who have indicated or whose clinical status or history would indicate they are currently taking high doses of biotin (> 10 mg per day). Biotin
levels of 2.5 µg/mL have been demonstrated to result in false negative test results.
- The extracted sample must be used within 4 hours of preparation when stored at room temperature.
- Refer to the CDC Interim Guidelines for Collecting, Handling, and Testing Clinical Specimens from Persons for Coronavirus Disease 2019 (COVID-19)
https://www.cdc.gov/coronavirus/2019-nCoV/lab/guidelines-clinical-specimens.html
Access bio care antigen US=FDA EUA Approvel LetterHK HuZef
est Principles
The CareStart™ COVID-19 Antigen Test is a lateral flow immunochromatographic assay for the detection of extracted nucleocapsid protein antigens specific to SARS-CoV-2 in nasopharyngeal and nasal swab specimens directly collected from individuals who are suspected of COVID-19 by their healthcare providers.
buy carestart covid-19 Antigen test by bio Access Inc, EUA Approvel Letter by...HK HuZef
buy carestart covid-19 Antigen test by bio Access Inc, EUA Approvel Letter by US-FDA for USA
Due to the highly contagious nature and global
health crises, SARS-CoV-2 has been designated
as a pandemic by the World Health Organization
(WHO) and continues to have devastating
impacts on healthcare systems and the world
economy including the U.S.
To effectively end the SARS-CoV-2 pandemic,
systematic screening and detection of clinical
COVID-19 cases is critical. An effective screening
regimen in a workplace, campus or other
community setting can be established using
the quick results provided by antigen tests.
As an intended point-of-care (POC) designated
test with a 10 min processing time, CareStart™
COVID-19 Antigen Test allows effective screening
of COVID-19 infection on a large scale.
Features
( Lateral flow assay
( No equipment required
( Rapid results within 10 minutes
( Minimally invasive specimen collection
(nasopharyngeal)
( Intended at POC setting by
medical professionals
Clinical Features
( Detect SARS-CoV-2 nucleocapsid
protein antigen with ultra high
performance
( Identify acute infection with high
sensitivity and 100% specificity
Test Principles
The CareStart™ COVID-19 Antigen Test is a lateral flow immunochromatographic assay for the detection of extracted nucleocapsid protein antigens specific to SARS-CoV-2 in nasopharyngeal and nasal swab specimens directly collected from individuals who are suspected of COVID-19 by their healthcare providers.
Accuracy report of Healgen IgG/IgM Rapid Test kits AntibodyHK HuZef
1. Accuracy
1.1 Purpose
To evaluate the equivalence of COVID-19 IgG/IgM Rapid Test result and clinical diagnosis.
1.2 Method
By testing clinical samples with known results, to evaluate the accuracy of COVID-19 IgG/IgM
Rapid Test (Whole blood/Serum/Plasma).
1.3 Material
COVID-19 IgG/IgM Rapid Test Cassette (Whole blood/Serum/Plasma)
Cassette Lot1: 2002155,Lot2:2002156,Lot3:2002157
Clinical samples
1.4 Test Procedure
1) Prepare specimens: tests were taken to clinical partner to evaluate, totally 113 specimens.
2) Perform the test according to the test procedure in the package insert
1.5 Result
1) IgM
Risk Analysis of igg/igm Rapid Test Kits by healgenHK HuZef
1. Introduction
1.1. Overview
This document provides a safety risk analysis for COVID-19 IgG/IgM Rapid Test Cassette (Whole
Blood/Serum/Plasma).
1.2. Intended Use/Purpose
COVID-19 IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma) is a solid phase immunochromatographic
assay for the rapid, qualitative and differential detection of IgG and IgM antibodies to 2019 Novel Coronavirusi in
human whole blood, serum or plasma. This test provides only a preliminary test result. Therefore, any reactive
specimen with the COVID-19 IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma) must be confirmed
with alternative testing method(s) and clinical findings.
1.3. Scope
This risk analysis addresses the safety risks that may affect the patient or the operator as associated with the
operation of the COVID-19 IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma).
This document provides a safety risk analysis for COVID-19 IgG/IgM Rapid Test Cassette (Whole
Blood/Serum/Plasma), which references the standard of ISO 14971:2012.
buy Healgen Antibody IgG/IgM rapid test kitsHK HuZef
COVID-19 IgG/IgM Rapid Test
COVID-19 IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma) is a solid
phase immunochromatographic assay for the rapid, qualitative and differential
detection of IgG and IgM antibodies to 2019 Novel Coronavirus in human whole
blood, serum or plasma.
HandleCFUS Series1000and all human blood products as though capable of transmitting infectious agents. -This product has not been FDA cleared or approved; -This product has been authorized by FDA under a EUA for use by laboratories certified under CLIA, that meet requirements to perform moderate or high complexity tests; -This product is only authorized for the duration of the declaration that circumstances
exist just if ying the authorization of emergency use of in vitro diagnostics for the detection and/or diagnosis of COVID-19 under Section 564(b)(1) of the Federal Food, Drug and Cosmetic Act, 21 U.S.C. § 360bbb-3(b)(1) unless the authorization is terminated or revoked sooner.
HandleCFUS Series1000and all human blood products as though capable of transmitting infectious agents. -This product has not been FDA cleared or approved; -This product has been authorized by FDA under a EUA for use by laboratories certified under CLIA, that meet requirements to perform moderate or high complexity tests; -This product is only authorized for the duration of the declaration that circumstances
exist just if ying the authorization of emergency use of in vitro diagnostics for the detection and/or diagnosis of COVID-19 under Section 564(b)(1) of the Federal Food, Drug and Cosmetic Act, 21 U.S.C. § 360bbb-3(b)(1) unless the authorization is terminated or revoked sooner.
Eua authorization letter for LYHER Covid-19 IgG/IgM Antibody rapid Test KitsHK HuZef
LYHER Covid-19 IgG/IgM Antibody Rapid test Kits, US FDA EUA Approval Certificate.
This product, CFUS Anti-SARS-CoV-2 Reference Material Kit Series 1000, is formulated for use with the LYHER Novel Coronavirus (2019-nCoV) IgM/IgG Antibody Combo Test Kit under the Emergency Use Authorization only.
CFUSSeries1000includes both antibody positive and negative reference materials. The positive is manufactured from human serum or plasma reactive for SARS-CoV-2IgG/IgM andnonreactiveforHBsAg and antibodies to HIV 1and 2, HTLV I and II, and HCV. There is a single vial of positive reference material (red cap) contained within each kit. The negative reference material is manufactured from human serum or plasma non-reactive for antibodies to SARS-CoV-2,aswellasHBsAgandantibodies to HIV 1 and 2, HTLV I and II, and HCV. There is a single vial of negative reference material (clear caps) contained within each kit. PACKAGE DETAIL:
Positive (Redcaps): 1x1.0mLvials Negative(Clearcaps): 1 x 1.0 ml vials This control contains stabilizers (EDTA, buffering agents), and 0.1% ProClin® (5- chloro-2-methyl- 4-isothiazolin-3-one & 2-methyl-4-isothiazolin-3-one) as preservative
Covid-19 Best test Kits Official partners in Nigeria Rescue Technologies LimitedHK HuZef
Online Selling Authorized on www.covid-19besttestkits.com officially made a sucessful deal for the sale of covid-19 rapid home test with kits Nigeria Rescue Technologies Limited
Rapid test kits approved by NAFDAC
9 Furo Ezimora street
+2348066861218
+234 802 423 0993
Rescue Technologies Limited is a private Emergency ambulance Armed Response company. We provide low-cost subscription based 24/7 availability as well as location based dedicated ambulance.
During this global pandemic we are assisting government and other organizations by providing covid-19 Rapid Tests as well as all all PPE our prices are better than competitive and fair and we are committed to keeping that way but prices continue to rise and supplies will become limited due th3 global demand
B4u Bravo cabs services in Pakistan, complete presentation for driversHK HuZef
b4u businees for you companies malysia and b4u trades launches complete best online texi cab service in pakistan and this is a complete presentation for online texi cab services frovided by bravo , these bravo cabs have extra services then other cabs ser5vicess avalable
this is the presentation prepared by Mr Muhammad Huzaifa A PHD Scholar and Founder , CEO Of MITZ " Mansehra Information technology Zone" and this presenation explaining the complete idea about humen resource management software . Mitz is one of the leading pakistani IT Commpany
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
New Drug Discovery and Development .....NEHA GUPTA
The "New Drug Discovery and Development" process involves the identification, design, testing, and manufacturing of novel pharmaceutical compounds with the aim of introducing new and improved treatments for various medical conditions. This comprehensive endeavor encompasses various stages, including target identification, preclinical studies, clinical trials, regulatory approval, and post-market surveillance. It involves multidisciplinary collaboration among scientists, researchers, clinicians, regulatory experts, and pharmaceutical companies to bring innovative therapies to market and address unmet medical needs.
HOT NEW PRODUCT! BIG SALES FAST SHIPPING NOW FROM CHINA!! EU KU DB BK substit...GL Anaacs
Contact us if you are interested:
Email / Skype : kefaya1771@gmail.com
Threema: PXHY5PDH
New BATCH Ku !!! MUCH IN DEMAND FAST SALE EVERY BATCH HAPPY GOOD EFFECT BIG BATCH !
Contact me on Threema or skype to start big business!!
Hot-sale products:
NEW HOT EUTYLONE WHITE CRYSTAL!!
5cl-adba precursor (semi finished )
5cl-adba raw materials
ADBB precursor (semi finished )
ADBB raw materials
APVP powder
5fadb/4f-adb
Jwh018 / Jwh210
Eutylone crystal
Protonitazene (hydrochloride) CAS: 119276-01-6
Flubrotizolam CAS: 57801-95-3
Metonitazene CAS: 14680-51-4
Payment terms: Western Union,MoneyGram,Bitcoin or USDT.
Deliver Time: Usually 7-15days
Shipping method: FedEx, TNT, DHL,UPS etc.Our deliveries are 100% safe, fast, reliable and discreet.
Samples will be sent for your evaluation!If you are interested in, please contact me, let's talk details.
We specializes in exporting high quality Research chemical, medical intermediate, Pharmaceutical chemicals and so on. Products are exported to USA, Canada, France, Korea, Japan,Russia, Southeast Asia and other countries.
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
Follow us on: Pinterest
Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...
neoplex pcr test kit [genematrix] neo_plex covid-19 detection kit_instruction for use_r
1. NR05A
NeoPlexTM
COVID-19 Detection kit
Multiplex Real-time PCR Reagents for 2019-novel coronavirus pathogens Detection
For professional in vitro diagnostic use only
[INTENDED USE]
The 'NeoPlex™ COVID-19 Detection Kit' Assay is a qualitative in vitro test for the simultaneous detection and
confirmation of N gene and RdRp gene in 2019-Novel Coronavirus causing COVID-19 from Respiratory specimens*
based on real-time reverse transcription polymerase chain reaction(RT-PCR) assay. This test kit is intended for
professional use.
* Respiratory specimens : Sputum, Bronchoalveolar lavage fluid(BAL), Nasopharyngeal or Oropharyngeal swab
[KIT CONTENTS] 96 Tests /Kit
Contents Volume(96T) Storage condition Shelf life
COVID-19 PPM
One-step Master Mix
COVID-19 PC
DW(RNase-free Water)
500 µL x 1Vial
500 µL x 1Vial
100 µL x 1Vial
1 mL x 1 Vial
-20 ℃ 5 months
[Compatible Instruments]
Applied Biosystems™ 7500 (Fast) Real-time PCR Instrument system (Thermo Fisher Scientific)
CFX96™ Real-time PCR detection system (Bio-Rad)
Gentier96 Real-time PCR System (Xi’an TianLong Science and Technology)
[Additional required equipment and materials]
0.2 ml 8-Tube PCR Strips without Caps, low profile, white (BioRad, Inc., Cat No. TLS0851)
Optical Flat 8-Cap Strips for PCR Tubes (BioRad, Inc., Cat No. TCS0803)
QIAamp DSP Viral RNA Mini Kit (QIAGEN,Cat No.61904) or equivalent nucleic acid extraction kit
Pipettes set, P2/P10, P20, P200, and P1000 aerosol barrier tips
Micro Centrifuge, Vortexer mixer
Disposable powder-free gloves
Use PCR plate strip caps only. Do not use PCR plate sealing film.
[KIT STORAGE AND STABILITY]
Store the kit below -20℃.
Kit materials are stable until the expiration date printed on the label under un-opened condition.
Kit’s shelf life is five (5) months.
Please use the reagents within four (4) weeks after opening.
1 / 9 NR05A-EN-IR1
2. NR05A
NeoPlexTM
COVID-19 Detection kit
Multiplex Real-time PCR Reagents for 2019-novel coronavirus pathogens Detection
For professional in vitro diagnostic use only
[WARNINGS AND PRECAUTIONS]
1. This device is intended for in vitro use only. Do not use the device for other purposes.
2. Wear personal protective equipments, such as gloves and lab coats when handling NeoPlexTM
COVID-19
Detection kit and/or specimens.
3. Do not smoke, drink or eat while handling NeoPlexTM
COVID-19 Detection kit and/or samples.
4. Please be careful when handling samples to prevent infections of user and/or indirect contact to a person.
Sample contains a risk of infections and unknown diseases.
5. Do not use reagents from different lots or from different tubes of the same lot.
6. If you do not frequently inspect the product, keep a kit in a refrigerator for a certain amount of time. Do not
freeze/thaw over four times. Repeated frozen/thawed product may result in false negative and false positive
results.
7. Be careful not to contaminate the product when extracting nucleic acid, amplifying PCR product, using positive
control (PC, Positive Control). The use of filter tips is recommended to prevent contamination of the product.
8. It is recommended that the sample or the positive control (PC, Positive Control) contained in the product to be
frozen and stored separately from the freezer storing the product.
9. Use the sterilized consumable laboratory supplies. Do not reuse it.
10. Add the extracted nucleic acid sample and positive control (PC, Positive Control) into the reaction solution in a
space separate from the PCR reaction solution preparation space.
11. Before using, read this instruction for use carefully.
12. Use calibrated measuring tools. (e.g. pipette)
13. Please check the expiration date before using the reagent.
14. Keep Positive Control separately when using to avoid contamination.
15. Before starting the PCR, make sure the lid is closed properly.
16. Dispose the product in accordance with local or national regulations.
17. Please consult with doctor about the test results.
2 / 9 NR05A-EN-IR1
3. NR05A
NeoPlexTM
COVID-19 Detection kit
Real-time RT-PCR Reagents for 2019-Novel Coronavirus (causing COVID-19) Detection
For professional in vitro diagnostic use only
[TEST PROCEDURE]
STEP 1. Preparation before testing
1) Preparation before testing
A. Prepare the all devices and reagent before use.
B. Place the kit under the room temperature at least 10 minutes before testing.
C. Mix well kit contents, and place them on ice before preparing PCR master mix.
Do not freeze/thaw over four times.
2) Specimen Collection, Transportation and Storage
A. Specimens for use: Respiratory specimens
B. Store specimens at 4 °C for no longer than seventy two (72) hours. For pro-longed storage, Freeze under
-70 °C condition.
C. Extracted nucleic acids should be stored at -70 °C or lower.
D. Transportation of clinical specimens must comply with local regulations for the transport of etiologic agents.
Use only the specimen type listed in the instruction manual.
The specimen volume should be above 0.5ml.
Wear eye protection, laboratory coats and disposable gloves when handling specimens.
Specimens should be stored under the storage conditions above. Otherwise, the wrong test results can be
obtained.
Sample information should be recorded to avoid confusion.
3 / 9 NR05A-EN-IR1
4. NR05A
NeoPlexTM
COVID-19 Detection kit
Real-time RT-PCR Reagents for 2019-Novel Coronavirus (causing COVID-19) Detection
For professional in vitro diagnostic use only
STEP 2. Nucleic acid extraction
After pre-treatment, nucleic acid extraction can be done by automated purification system or using manual prep kits
(QIAamp DSP Viral RNA Mini Kit or equivalent).
1) Pre-treatment of theSpecimen
BAL, Naso/Oropharyngeal swab Sputum
Place the specimen at room temperature Place the specimen at room temperature
Prepare the sample by vortexing for 20 seconds or more
before use.
Add saline or PBS to the specimen (1 of specimen: 2 of
saline or PBS) and vortex it for 1 minute.
Leave it at room temperature for 20 minutes.
Vortex it for 30 seconds
4 / 9 NR05A-EN-IR1
2) For nucleic acid extraction, follow the manufacturer'sprotocol.
We recommend QIAamp DSP Viral RNA Mini Kit or equivalent nucleic acid extraction kit/automatic machine for
nucleic acid extraction.
STEP 3. Prepare PCR Master Mix and sample
1) Prepare the PCR MasterMix
Contents Volume per test
COVID-19 PPM 5ul
One-step Master Mix 5ul
DW(RNase-free Water) 5ul
Total Volume 15ul
Note : Calculate the required amount of each reagent based on the number of reactions (samples + controls).
2) Vortex and briefly centrifuge the PCR Master Mix.
3) Place 15μL aliquots of the PCR Master mix into 0.2ml PCR tubes, and close the lids.
4) Add 5μl of each nucleic acid sample to its respective tube.
Contents 1 test (Volume)
PCR Master Mix 15ul
Nucleic acid sample 5ul
Total Reaction Volume 20ul
It is recommended that the PCR mixture to be prepared just before use.
Aerosol-resistant filter tips and tight gloves should be used when preparing samples. Take great care to avoid
cross contamination.
Defrost the reagents completely
Centrifuge the reagent tubes briefly to remove the drops from the inside of the lids.
5. NR05A
NeoPlexTM
COVID-19 Detection kit
Real-time RT-PCR Reagents for 2019-Novel Coronavirus (causing COVID-19) Detection
For professional in vitro diagnostic use only
5) Make the control amplification reactions
- Negative Control(NC): Add 5μl of DW (RNase-free water (devided)) instead of nucleic acid samples to the tube
- Positive Control(PC): Add 5μl of COVID-19 PC instead of nucleic acid samples to the tube
Use a new pipette tip with each different sample.
Avoid cross-contamination of PCR Master mix and samples with Positive Control.
Do not label on the cap of the reaction tubes as fluorescence is detected through the cap.
Centrifuge the PCR tube thoroughly for 30 seconds
STEP 4. PCR
1) Selection of fluorescencechannels
Instruments RdRp gene N gene IC
CFX96 FAM HEX Cy5
ABI 7500 (Fast) FAM JOE Cy5
Gentier96 FAM HEX Cy5
2) Setting the PCRprotocol
PCR protocol should be set according to the table below.
Segment Temperature (°C) Time Cycles
1 50 30 min 1
2 95 15 min 1
3 95 15 sec
40
4* 60 60 sec
* Segment 4: Fluorescence data should be collected during the 60°C incubation step
STEP 5. Test result analysis
For the analysis of the test result after PCR amplification, take the Ct* result and interpret the according to the
following table.
[INTERPRETATION OF TEST RESULTS]
For the analysis of the test result after PCR amplification, take the amplification curve (or amplification plot) result
(For ABI 7500(Fast) and GENTIER96, check the ‘Analysis’ tab and for CFX96, check the ‘Quantitation’ tab) and
interpret the according to the following interpretation table.
5 / 9 NR05A-EN-IR1
6. NR05A
NeoPlexTM
COVID-19 Detection kit
Real-time RT-PCR Reagents for 2019-Novel Coronavirus (causing COVID-19) Detection
For professional in vitro diagnostic use only
1. Interpretation criteria for result analysis
Target fluorescence Ct * Interpretation
COVID-19 RdRp gene FAM
≤ 38
>38 or N/A
Positive (+)
Negative (-)
COVID-19 N gene JOE or HEX
≤ 38
>38 or N/A
Positive (+)
Negative (-)
≤ 38 Positive (+)
IC** Cy5
>38 or N/A Negative (-)
* Ct: threshold cycle value
** The Internal Control (IC) gene is to monitor the nucleic acid isolation procedure and the possibility of PCR inhibition.
2. Interpretation of result
FAM JOE or HEX Cy5
Positive Negative
Case
RdRp gene N gene IC*
Control Control Interpretation
1 + + + + - COVID-19
2 + + - + - COVID-19
3 - - + + - Negative
4 - - - + -
5 +/- +/- +/- + -
6 +/- +/- +/- + + Invalid/Re-test
7 +/- +/- +/- - +
8 +/- +/- +/- - -
6 / 9 NR05A-EN-IR1
* IC is not necessary for the interpretation of positive or negative results and high load of pathogen’s nucleic acid results in the low signal or
negative signal of IC.
[Quality Control]
NeoPlex™ COVID-19 Detection Kit includes COVID-19 PC as positive control and DW(RNase-free water) as
negative control. For all runs, valid test results must be obtained for both Positive and Negative control. Positive
Control result must be Positive (Valid). Negative Control result must be Negative (Valid). If the positive and negative
control results are consistently invalid, contact us for technical assistance.
7. NR05A
NeoPlexTM
COVID-19 Detection kit
Multiplex Real-time PCR Reagents for 2019-novel coronavirus pathogens Detection
For professional in vitro diagnostic use only
[TROUBLE SHOOTING]
NeoPlex™ COVID-19 Detection Kit includes COVID-19 PC as positive control and DW(RNase-free water) as
negative control. For all runs, valid test results must be obtained for both Positive and Negative control. Positive
Control result must be Positive (Valid). Negative Control result must be Negative (Valid). If the positive and negative
control results are consistently invalid, contact us for technical assistance.
1. If the Internal control signal is not observed
7 / 9 NR05A-EN-IR0
Potential causes Solution
Error in specimen collection If the both target and IC signal were not observed, recollect the specimen
Nucleic acid extraction failure
Read carefully the instruction for use of nucleic acid extraction kit and extract the
nucleic acid from specimen again.
IC may be mixed with 10 μl during the extraction process.
Incorrect PCR setting Repeat the detection procedure with a correct setting
Incorrect PCR cycle or machine temperature Check the PCR conditions and repeat the PCR under the correct setting if necessary
The fluorescence for data analysis do not comply
with the protocol
Select the correct fluorescence for each target listed in this Instruction guide for data
analysis
Leaving reagents at room temperature for a long
time or incorrect storage condition
Check the storage conditions and the expiration date of the reagents and use a new kit
Presence of inhibitor
Dilute the template nucleic acid in distilled water (10-100x) and repeat the PCR with
the diluted nucleic acid (If specimen is still present, restart from nucleic acid extraction
procedure)
High load of pathogen’s nucleic acid
Dilute the template nucleic acid in distilled water (10-100x) and repeat the PCR with
the diluted nucleic acid
2. If signals are observed at the negative control / false positive
Potential causes Solution
Presence of cross contamination
Decontaminate all surfaces and instruments with sodium hypochlorite or ethanol. Use
filter tips during the extraction procedure. Change tips among tubes. Repeat the
nucleic acid extraction with the new set of reagents
3. If no signal is observed at the positive control / false negative
Potential causes Solution
Error in specimen collection Recollect the specimen
Incorrect storage of the specimen
Recollect the specimen and repeat the whole process. Make sure the product is stored
in recommended conditions
Error in nucleic acid extraction Re-extract the nucleic acid
Incorrect PCR setting Repeat the PCR with corrected setting
Error in adding nucleic acid to corresponding PCR
tubes
Check the sample numbers for nucleic acid containing tubes and make sure to add
nucleic acid into correct PCR tubes during detection process.
Incorrect PCR mixture
Check whether all components are added or not (If you use to pre-composed premix,
should be reduce sensitivity) Each reagents should be used after homogenization and
spin down reagent tube before put the real-time PCR
8. NR05A
NeoPlexTM
COVID-19 Detection kit
Multiplex Real-time PCR Reagents for 2019-novel coronavirus pathogens Detection
For professional in vitro diagnostic use only
[LIMITATION OF TEST]
1. Results from this test must be correlated with the clinical history, epidemiological data, and other data of the
patient available to the clinician.
2. If you do not use the samples and other specimens described in this manual, you may get inaccurate results.
3. Although the results of this test are negative, it is not advisable to exclude the possibility that the infection is
actually present.
4. It is not excluded that this kit shows false positive results due to the presence of cross-contamination.
5. False negative results may occur due to polymerase inhibition. COVID-19 IC may help to identify any substance
existing in the specimens interfering with nucleic acid isolation and PCR amplification.
6. This kit is for professional use only. Only trained healthcare provider can use this kit.
8 / 9 NR05A-EN-IR0
9. NR05A
NeoPlexTM
COVID-19 Detection kit
Multiplex Real-time PCR Reagents for 2019-novel coronavirus pathogens Detection
For professional in vitro diagnostic use only
[SYMBOLS]
Catalogue number Batch code Date of manufacture Use-by date
In vitro diagnostic medical
device
Upper limit of temperature Caution Consult instruction for use
Manufacturer
Contains sufficient for <n>
tests
Authorized representative in
the European Community
Conformity to European
Directive 98/79/EC
Issue date: 2020.03.
9 / 9 NR05A-EN-IR0