Talk describing our resource activity to the scientific advisory board of a similar resource in England that was the subject of a European design study, known as DGEmap.

1. Service de Cytogénétique & d’Embryologie Hôpital Necker-Enfants Malades M Vekemans The Necker Human Embryo Resource Foetal pathology Embryology Molecular Genetics G Goudefroye H Etchevers S Thomas C Babarit T Attié-Bitach F Encha-Razavi M Bonnière C Esculpavit J Martinovic

3. Mostly RU-486 Occasionally aspiration Embryos < 50 days 5-9 wg C9-C19 Late embryos/early foetuses 8-12 wg C18-23+ whole embryonic sac 60% recovery entire / pieces fragments organs, parts never whole brain Techniques for elective abortion Whole embryo with trophoblast Pieces (spinal cord)

6. Distribution of embryo ages

8. Gene expression: in situ hybridization FGF10 , C19 NANOG , C15

9. Gene expression: SAGE and microarrays

10. Gene expression: (q)RT-PCR

11. Gene translation: immunohistochemistry Synaptogamin (brown) in intestine Neural cell adhesion molecule (white) in nerves and spinal cord

14. © Nick Kim, http://nearingzero.net/