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Component-2 “Production-to-Consumption”


   A Value Chain on Composite Dairy Foods with
            Enhanced Health Attributes




Project Sanctioned: 1st, March, 2009      Completion Date: 1st, June, 2012


National Dairy Research Institute, Karnal
Central Institute of Post Harvest Engineering & Technology, Ludhiana
Arpana Research & Charities Trust, Madhuban, Karnal
                     Total Budget Outlay: 283.076 Lakhs
                                    Website:          www.compositefoods.org.in
Objectives
 To harness the nutritional and therapeutic potential of
   milk by-products and underutilized plant species
  (pearl millet and barley) for development of
  functional foods

 To develop technological package for composite dairy
  foods (complementary foods, fortified convenience
  foods and probiotic milk-cereal foods) with enhanced
  health attributes

 To validate the consumer acceptability and targeted
  health benefits of composite dairy foods

 To assess the techno-economic feasibility of the newly
  developed technologies through linkages with
  industry, marketing personnel and Self-help group
Primary Processing Equipments
• Millet Pearler developed by CIPHET, Ludhiana and
  the performance evaluation of the machine is
  completed
• It is quite useful for the manufacture of primary
  processed products like grits, semolina and flour
  from Pearl millet
• Problems of low shelf-life, unattractive colour and
  higher tannin/phenolics could be simply solved by
  hydrothermal treatment
• Process of germination and roasting could also be
  applied to enhance the nutritional profile and
  organoleptic quality of millet crops
Some equipments/machines for primary processing of
            pearl millet and barley.




  DESTONER        CLEANER CUM         PEARLER
                    GRADER
Convenience Products
• Processing technology for the manufacture of
  convenience mixes for Halwa and Upma has been
  optimized
• These mixes can be utilized at home scale as well
  for Institutional catering to save time
• Pearl millet pasta enriched with whey protein has
  been developed. Addition of hydrocolloids improved
  the quality attributes of the product
• Test marketing of these products indicate that the
  acceptability of these products could further be
  improved if they are used in combination with
  wheat grits or flour
Snack Foods




• Formulation and technology to produce protein-enriched
  extruded snack has been completed.
• Biscuits of highly acceptable quality is made from a
  formulation of composite dairy-cereal mix consisting of
  whey protein concentrate, pearl millet flour and refined
  wheat flour
• Optimized biscuits will provide 15.6% of calorie, 20%
  protein, 7.6% iron and 9% of daily calcium requirement of
  RDA per 100 g of product.
Pearl Millet-Whey Based Complementary
                      Food
•   Whey & Skim milk blend in the ratio of 70:30 or 80:20 can
    be utilized for complementary food base material
•   Germination of Pearl millet grains for 48 hr followed by
    roasting decrease phytic acid level and also results in higher
    amylase activity.
•   Optimum levels of pearl millet, barley malt extract and
    maltodextrin in the complementary food is 20 %, 15 % and
    5.7% respectively
•   The product developed was readily reconstitutable and at
    9% level of reconstitution, it was highly acceptable
•   The product was nutritionally superior in terms of minerals,
    protein and digestibility
•   Protein, moisture, ash, carbohydrate and microbial quality
    was according to PFA rules (2008) for milk-cereal based
    complementary foods.
Pearl Millet-Whey-Skim Milk
    Complementary Foods




   Spray Dried          Tray Dried
Complementary Food   Complementary Food
Pearl Millet based Beverage (Bajra Lassi)
• Suitable bacterial starter have
  been identified that can ferment
  milk as well as millet solids
• Process of fermentation reduced
  the level of phytate and improved
  the bioavailability of minerals
Milk-Millet Based Probiotic Food
• Millet components perform number of functions in
  probiotic consortium
      Provide fermentable substrates   for   growth   of
       probiotic microorganisms
      As prebiotics due to presence of non-digestible
       carbohydrates

• Malt and extracts of millet showed significant
  protective effect on L. plantarum & L. acidophilus
  strains under acidic conditions

• Presence of certain compounds like fermentable sugar,
  free amino acid favour the growth of probiotic microbes
  in millet based substrate
Popularization of Developed Products
• Development of publicity material related to nutritional
  and therapeutic potential of Pearl millet & whey
• Brochure and Posters of the products developed in the
  project
• Development of a website www.compositefoods.org.in for
  disseminating the information through ICT tools
• Participation in more than 20 exhibitions at various places
  of the country to display our products and distribution of
  samples
• Test marketing in collaboration with industry and
  prospective entrepreneurs for the developed products
• Publication of articles on composite foods in print media.
  Demonstration of technologies and products on electronic
  media
Market Access to Technology
• Test marketing of instant mixes, pasta from
  CIPHET, Ludhiana and Bajra lassi &
  biscuits from NDRI Parlour has been started
• One Integrated processing unit has started
  the production of composite dairy foods at
  Amritpur Kalan, near Karnal
• Linkage with Technology Business
  Incubator (TBI) and ZTM-BPD Units of
  ICAR for technology transfer and
  entrepreneurship development
Rural Enterprise on Composite Foods




Unit is involved in Manufacturing & Marketing of dairy
products including whey based drinks, Bajra lassi and
Biscuits, run by 14 resource poor women
Observation of Beneficiaries/Stakeholders
• 32 entrepreneurs have registered with TBI for training
  and Technology Business incubation
• Bhartiya Kisaan Union (BKU) of Haryana has indicated
  their willingness to promote the primary processing of
  Pearl millet in Jind District of Haryana
• Invited to make special presentation during the 46th All
  Indian Coordinated Project on Pearl Millet Improvement
  at Hisar
• Invited to make special presentation at National KVKs
  Conference held at Udaipur, Rajasthan
• Danone India Pvt. Ltd. is working for the establishment of
  rural enterprise in similar way as developed in project
• Britannia India Ltd. New Delhi are willing to start certain
  composite dairy products developed in project.
Cross Consortia Learning
• Strengthening the linkage with stakeholders for
  promoting the production, consumption and value
  addition through composite dairy foods

• Assistance in promotion of developed products in
  Northern states through display of publicity material

• Development    of   technological  packages   for
  milk/sorghum/small millet products like lassi,
  extruded snacks and complementary foods at NDRI

• Assistance in organization of “Entrepreneurship
  Development Programme” at NDRI, Karnal for the
  benefit of interested personnel
Outreach Programmes
• Inclusion of Post Harvest Processing & Value
  addition as major activity in 12th Plan of All
  India Coordinated Research Project on Pearl
  Millet Improvement

• Identification of “Composite Dairy Foods”
  based on Millets & minor cereals as Major
  Research Programme for the next 5-year
  plan of NDRI, Karnal

• Submitted project in “Milk-Millet” based
  composite probiotic foods under Indo-
  Finnish Cooperation
Cross Consortia Learning
• Strengthening the linkage with stakeholders for
  promoting the production, consumption and value
  addition through composite dairy foods

• Assistance in promotion of developed products in
  Northern states through display of publicity material

• Development    of   technological  packages   for
  milk/sorghum/small millet products like lassi,
  extruded snacks and complementary foods at NDRI

• Assistance in organization of “Entrepreneurship
  Development Programme” at NDRI, Karnal for the
  benefit of interested personnel
Experience & Lesson Learned
• Experience is quite encouraging as there is
  increasing awareness among stakeholders
  regarding millets and dairy by-products
• Incorporation of millets with milk/milk by-
  products not only improve nutritional profile
  but also enhance consumer acceptability
• Certain products like millet based bakery
  products, snacks and beverages could attract
  large consumer base
• Technology transfer mechanisms are quite
  cumbersome and discourage the industry and
  entrepreneurs
Antimicrobial Activity
Salmonella                     Listeria Monocytogens




                     E.Coli




                                     B.Cereus
S.Aureus
Antifungal activity
Kluveromyces marxianus   Saccharomyces cerevisiae      Aspergillus




        Candida                 Rhizopus            Kluveromyces Lactis
Procedure
- L.rhamnosus RSI-3 has shown good survivalability at
  different pH, and different concentration of Bile salts in
  in-vitro treatment

- L.rhamnosus    RSI-3     shown     good    cell   surface
  hydrophobicity

- L.rhamnosus RSI-3 has exhibited best antifungal activity,
  antimicrbial activity and antibiotic sensitivity against
  various antibiotics

- L.rhamnosus RSI-3 produces sufficient β-galactsidase
  enzymes and has ability to deconjugate bile salts
• Probiotic fed group of Rats significantly increased level of
  haemoglobin as compared to Normal             control and
  Diabetic control group

• Probiotic fed group of rats significantly increased level of
  antioxidative enzymes in liver and RBCs such as
  Catalase, SOD and GPx

• Probiotic fed group of rats slightly improved lipid
  profiles, including the reduction of Serum/Plasma total
  cholesterol, LDL-cholesterol, VLDL-Cholesterol and
  triglycerides or increment of HDL-Cholesterol.
 The present investigation has culminated in establishment of
  potential probiotic attributes of RSI3, a native strain
  bioprospected from a traditional cereal based fermented
  milk product.
 This strain has shown better in vitro probiotic attributes vis –
  a- vis LGG.
 In animal model, this strain has shown as a good antidiabetic
  effect in terms of ---------and better in terms of ……….in
  comparison to LGG.
 This study has shown that probiotic therapy is effective in
  control of diabetes.
 This strain is a prolific grower typical of probiotic organism
  which are invariably slow grower and has the potential to be
  used as commercial probiotic strain with due validation and
  has the techno-functional attributes for its use in
  manufacture OF a functional dairy food.
Ndri composite experience-presentation

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Ndri composite experience-presentation

  • 1. Component-2 “Production-to-Consumption” A Value Chain on Composite Dairy Foods with Enhanced Health Attributes Project Sanctioned: 1st, March, 2009 Completion Date: 1st, June, 2012 National Dairy Research Institute, Karnal Central Institute of Post Harvest Engineering & Technology, Ludhiana Arpana Research & Charities Trust, Madhuban, Karnal Total Budget Outlay: 283.076 Lakhs Website: www.compositefoods.org.in
  • 2. Objectives  To harness the nutritional and therapeutic potential of milk by-products and underutilized plant species (pearl millet and barley) for development of functional foods  To develop technological package for composite dairy foods (complementary foods, fortified convenience foods and probiotic milk-cereal foods) with enhanced health attributes  To validate the consumer acceptability and targeted health benefits of composite dairy foods  To assess the techno-economic feasibility of the newly developed technologies through linkages with industry, marketing personnel and Self-help group
  • 3. Primary Processing Equipments • Millet Pearler developed by CIPHET, Ludhiana and the performance evaluation of the machine is completed • It is quite useful for the manufacture of primary processed products like grits, semolina and flour from Pearl millet • Problems of low shelf-life, unattractive colour and higher tannin/phenolics could be simply solved by hydrothermal treatment • Process of germination and roasting could also be applied to enhance the nutritional profile and organoleptic quality of millet crops
  • 4. Some equipments/machines for primary processing of pearl millet and barley. DESTONER CLEANER CUM PEARLER GRADER
  • 5. Convenience Products • Processing technology for the manufacture of convenience mixes for Halwa and Upma has been optimized • These mixes can be utilized at home scale as well for Institutional catering to save time • Pearl millet pasta enriched with whey protein has been developed. Addition of hydrocolloids improved the quality attributes of the product • Test marketing of these products indicate that the acceptability of these products could further be improved if they are used in combination with wheat grits or flour
  • 6. Snack Foods • Formulation and technology to produce protein-enriched extruded snack has been completed. • Biscuits of highly acceptable quality is made from a formulation of composite dairy-cereal mix consisting of whey protein concentrate, pearl millet flour and refined wheat flour • Optimized biscuits will provide 15.6% of calorie, 20% protein, 7.6% iron and 9% of daily calcium requirement of RDA per 100 g of product.
  • 7. Pearl Millet-Whey Based Complementary Food • Whey & Skim milk blend in the ratio of 70:30 or 80:20 can be utilized for complementary food base material • Germination of Pearl millet grains for 48 hr followed by roasting decrease phytic acid level and also results in higher amylase activity. • Optimum levels of pearl millet, barley malt extract and maltodextrin in the complementary food is 20 %, 15 % and 5.7% respectively • The product developed was readily reconstitutable and at 9% level of reconstitution, it was highly acceptable • The product was nutritionally superior in terms of minerals, protein and digestibility • Protein, moisture, ash, carbohydrate and microbial quality was according to PFA rules (2008) for milk-cereal based complementary foods.
  • 8. Pearl Millet-Whey-Skim Milk Complementary Foods Spray Dried Tray Dried Complementary Food Complementary Food
  • 9. Pearl Millet based Beverage (Bajra Lassi) • Suitable bacterial starter have been identified that can ferment milk as well as millet solids • Process of fermentation reduced the level of phytate and improved the bioavailability of minerals
  • 10. Milk-Millet Based Probiotic Food • Millet components perform number of functions in probiotic consortium  Provide fermentable substrates for growth of probiotic microorganisms  As prebiotics due to presence of non-digestible carbohydrates • Malt and extracts of millet showed significant protective effect on L. plantarum & L. acidophilus strains under acidic conditions • Presence of certain compounds like fermentable sugar, free amino acid favour the growth of probiotic microbes in millet based substrate
  • 11. Popularization of Developed Products • Development of publicity material related to nutritional and therapeutic potential of Pearl millet & whey • Brochure and Posters of the products developed in the project • Development of a website www.compositefoods.org.in for disseminating the information through ICT tools • Participation in more than 20 exhibitions at various places of the country to display our products and distribution of samples • Test marketing in collaboration with industry and prospective entrepreneurs for the developed products • Publication of articles on composite foods in print media. Demonstration of technologies and products on electronic media
  • 12. Market Access to Technology • Test marketing of instant mixes, pasta from CIPHET, Ludhiana and Bajra lassi & biscuits from NDRI Parlour has been started • One Integrated processing unit has started the production of composite dairy foods at Amritpur Kalan, near Karnal • Linkage with Technology Business Incubator (TBI) and ZTM-BPD Units of ICAR for technology transfer and entrepreneurship development
  • 13. Rural Enterprise on Composite Foods Unit is involved in Manufacturing & Marketing of dairy products including whey based drinks, Bajra lassi and Biscuits, run by 14 resource poor women
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  • 16. Observation of Beneficiaries/Stakeholders • 32 entrepreneurs have registered with TBI for training and Technology Business incubation • Bhartiya Kisaan Union (BKU) of Haryana has indicated their willingness to promote the primary processing of Pearl millet in Jind District of Haryana • Invited to make special presentation during the 46th All Indian Coordinated Project on Pearl Millet Improvement at Hisar • Invited to make special presentation at National KVKs Conference held at Udaipur, Rajasthan • Danone India Pvt. Ltd. is working for the establishment of rural enterprise in similar way as developed in project • Britannia India Ltd. New Delhi are willing to start certain composite dairy products developed in project.
  • 17. Cross Consortia Learning • Strengthening the linkage with stakeholders for promoting the production, consumption and value addition through composite dairy foods • Assistance in promotion of developed products in Northern states through display of publicity material • Development of technological packages for milk/sorghum/small millet products like lassi, extruded snacks and complementary foods at NDRI • Assistance in organization of “Entrepreneurship Development Programme” at NDRI, Karnal for the benefit of interested personnel
  • 18. Outreach Programmes • Inclusion of Post Harvest Processing & Value addition as major activity in 12th Plan of All India Coordinated Research Project on Pearl Millet Improvement • Identification of “Composite Dairy Foods” based on Millets & minor cereals as Major Research Programme for the next 5-year plan of NDRI, Karnal • Submitted project in “Milk-Millet” based composite probiotic foods under Indo- Finnish Cooperation
  • 19. Cross Consortia Learning • Strengthening the linkage with stakeholders for promoting the production, consumption and value addition through composite dairy foods • Assistance in promotion of developed products in Northern states through display of publicity material • Development of technological packages for milk/sorghum/small millet products like lassi, extruded snacks and complementary foods at NDRI • Assistance in organization of “Entrepreneurship Development Programme” at NDRI, Karnal for the benefit of interested personnel
  • 20. Experience & Lesson Learned • Experience is quite encouraging as there is increasing awareness among stakeholders regarding millets and dairy by-products • Incorporation of millets with milk/milk by- products not only improve nutritional profile but also enhance consumer acceptability • Certain products like millet based bakery products, snacks and beverages could attract large consumer base • Technology transfer mechanisms are quite cumbersome and discourage the industry and entrepreneurs
  • 21. Antimicrobial Activity Salmonella Listeria Monocytogens E.Coli B.Cereus S.Aureus
  • 22. Antifungal activity Kluveromyces marxianus Saccharomyces cerevisiae Aspergillus Candida Rhizopus Kluveromyces Lactis
  • 24. - L.rhamnosus RSI-3 has shown good survivalability at different pH, and different concentration of Bile salts in in-vitro treatment - L.rhamnosus RSI-3 shown good cell surface hydrophobicity - L.rhamnosus RSI-3 has exhibited best antifungal activity, antimicrbial activity and antibiotic sensitivity against various antibiotics - L.rhamnosus RSI-3 produces sufficient β-galactsidase enzymes and has ability to deconjugate bile salts
  • 25. • Probiotic fed group of Rats significantly increased level of haemoglobin as compared to Normal control and Diabetic control group • Probiotic fed group of rats significantly increased level of antioxidative enzymes in liver and RBCs such as Catalase, SOD and GPx • Probiotic fed group of rats slightly improved lipid profiles, including the reduction of Serum/Plasma total cholesterol, LDL-cholesterol, VLDL-Cholesterol and triglycerides or increment of HDL-Cholesterol.
  • 26.  The present investigation has culminated in establishment of potential probiotic attributes of RSI3, a native strain bioprospected from a traditional cereal based fermented milk product.  This strain has shown better in vitro probiotic attributes vis – a- vis LGG.  In animal model, this strain has shown as a good antidiabetic effect in terms of ---------and better in terms of ……….in comparison to LGG.  This study has shown that probiotic therapy is effective in control of diabetes.  This strain is a prolific grower typical of probiotic organism which are invariably slow grower and has the potential to be used as commercial probiotic strain with due validation and has the techno-functional attributes for its use in manufacture OF a functional dairy food.