1. Simultaneous use of microarray expression data
The analysisof gene expressionmicroarraydatausingclusteringtechniquestoimprove the data
service howeverattentions are nowturningtomodel basedapproaches the tissue space andthe
gene space are generallyquite differentdue tothe dimensions of tissue growththe clusteringof the
tissuesonthe basisof the gene is therefore anonstandard problemof clustergrowth
One way tohandle this dimensionally problemistoignore the correlationsbetweenthe genesto
clustersthe tissue samplesbye fittingmixturesof abnormal components innew bacteriacompounds
withdiagonal covariance matrices.Thisisessential equivalenttousingthe k-meansclustering
procedures intissues samples,howeverthis assumption of uncorrelatedgenes leadstospherical
clusterswhere,inpractice the clusterstendtobe elliptical due tothe correlationsthat extendtobe
elliptical due tothe data correlationsthatexistbetweensome of the gene samples.Whichenables
elliptical clusterstobe imposedonthe tissuessamples.Withamixture model-basedapproachto
clustering,the gcomponents inthe mixture model are conceptualised asrepresentingthe external
classedcorrespondingtothe g clusters to be imposedonthe data.Once the mixture model has been
fitted,aprobabilitiesof competentmembershipforthe tissue data,anoutrightassignmenteach
data setcorrespondingtothe g clusteris achieved byassigningeachdatapointto the componentto
whichithas the highestestimatedprobabilityof belonging.The questionof how manygenuine
tissue clustersetsgissupported bythe data can be considered intermsof the change intissue
growth.
The extensiontothe presentcase where the dimensions of the feature vectorthe numbersof genes
isso much greaterthan the outputof data sets to be formedasclusterbasednumberof tissue
samples ishandledintoways.Firstlygenesare screened onanindividual basisto eliminate those
whichhave little variationacrossthe tissue samples intermsof likely hoodtoratios,thenthe
retainedgenesare formedintoclusterssetsforminginadecreasingorderof the clustercapacityof
theirmeanings therefore groupsare neededtoexploretissuesamplesforanyclassor sub classof
data structure that isthe meansof groupsintowhichthe geneshave beenclusteredprovidea useful
representationof the genesinalower diametricspace the dimensionof the space isequal tothe
data setsinthe providednumberof groupsets.The use of the latterreducesthe numberof
parametersinthe tissue byimposingthe assumption thatthe correlationsbetweenthe genescanbe
expressed inaloweroutputbythe dependence of the tissue onasmall numberof unobservable
factors includingthe recognition sequences specificdnaproteinsinteractionsare very goodinthe
regulates networkof the geno.The exactrulesof these interactionsare notwell understood.The
knowformsof dnathe double helix are closed,inverted structureswhere molecularinformationis
not directly exposedonasurface or tissue, howeverthe majorgrove isrichin chemical information.
The edgesof each base data setsare exposedinmajorand minorgrooves,creatingapatternof new
hydrogen bondtissues. If all the testgenesare assumedindependentthe samplescanhave
generatedbyindependentlysampling,Inanexampleof atwo sample clusterproblem bycalculating
the observedlevel of significance of the test staticscalculate usingrandomresampledtissuevectors
of the observationsof gene expressionsfromthe original datasets,Howeverthere will be overlaps
these are calledpairwise overlapsthese are commonlyfoundindatasetsof a hole clusterof a
chromosome these become mutatedconnectedthrough transitive pairwiseoverlappingof the data
setsafterthe internal quality filteringare firstgroupedinto clusters eachof whichholdcommon
property’s sequencesthatoverlapswith all othersequencesinthe clustersthussuperclusters.
Superclustersare formedbygroupingtogetherall clustersthatshare one ormore data sequences or
tissue fragments fromadifferentclusterdatasetthus,overlapvalidationviasequenceslayoutneeds
onlyto be performedwithineachsupercluster,greatlyreducingcomputation. Efficientoverlap
2. validation viasequenceslayoutrequiresrapidretrieval of apairwise overlapinformation,forwhich
we use hash tables.Once superclustershave beengenerated,one hashtable canstore all the
pairwise overlapsforeachsupercluster,usingpairwise sequence idsasthe keys.
Overlapvalidationiscarriedoutbybuildingasequence coordinatesystemforeachclusterinthe
supercluster.The system assignsapaircoordate data setsto each tissues segmentsbasedonits
overlappositionwiththe commonsubject sequences these determinethe certain base positionof
the sequences the cordationsystemspecifiesthe orientation.The commonsequences setatthe
originwithcoordinate while coordate foranyothersequencesinthe tissueclusterisdeterminedby
where the dataholdsand are definedasdifferentclustersinthe same superclustershave different
origins.
