Genetic markers, Classical markers, DNA markers, MICROSATELLITES, AFLP, SNP: Single Nucleotide Polymorphism, QTL: Quantitative Trait Locus, Activities of marker-assisted breeding, Marker-based breeding and conventional breeding Perspectives,The application of molecular technologies to plant breeding is still facing the following drawbacks and/or challenges
Process whereby a marker is used for indirect selection of a genetic determinant or determinants of a trait of interest (i.e. productivity, disease resistance, abiotic stress tolerance, and/or quality).
Trait of interest is selected not based on the trait itself but on a marker linked to it.
The assumption is that linked allele associates with the gene and/or quantitative trait locus (QTL) of interest. MAS can be useful for traits that are difficult to measure, exhibit low heritability, and/or are expressed late in development.
Pre-Requisites: Two pre-requisites for marker assisted selection are: (i) a tight linkage between molecular marker and gene of interest, and (ii) high heritability of the gene of interest.
Markers Used: The most commonly used molecular markers include amplified fragment length polymorphisms (AFLP), restriction fragment length polymorphisms (RFLP), random amplified polymorphic DNA (RAPD), simple sequence repeats (SSR) or micro satellites, single nucleotide polymorphisms (SNP), etc. The use of molecular markers differs from species to species also.
Genetic markers, Classical markers, DNA markers, MICROSATELLITES, AFLP, SNP: Single Nucleotide Polymorphism, QTL: Quantitative Trait Locus, Activities of marker-assisted breeding, Marker-based breeding and conventional breeding Perspectives,The application of molecular technologies to plant breeding is still facing the following drawbacks and/or challenges
Process whereby a marker is used for indirect selection of a genetic determinant or determinants of a trait of interest (i.e. productivity, disease resistance, abiotic stress tolerance, and/or quality).
Trait of interest is selected not based on the trait itself but on a marker linked to it.
The assumption is that linked allele associates with the gene and/or quantitative trait locus (QTL) of interest. MAS can be useful for traits that are difficult to measure, exhibit low heritability, and/or are expressed late in development.
Pre-Requisites: Two pre-requisites for marker assisted selection are: (i) a tight linkage between molecular marker and gene of interest, and (ii) high heritability of the gene of interest.
Markers Used: The most commonly used molecular markers include amplified fragment length polymorphisms (AFLP), restriction fragment length polymorphisms (RFLP), random amplified polymorphic DNA (RAPD), simple sequence repeats (SSR) or micro satellites, single nucleotide polymorphisms (SNP), etc. The use of molecular markers differs from species to species also.
Role of Marker Assisted Selection in Plant Resistance RandeepChoudhary2
Topic Role of Marker Assisted Selection in Plant Resistance is described in detail including some case studies.
Types of markers used in genetic engineering and biotechnology are described in detail.
Marker assisted selection is a process whereby a marker (morphological, biochemical or one
based on DNA/RNA variation) is used for indirect selection of a genetic determinant of a trait
of interest. Since the first reported linkage of an agronomically important trait (a quantitative
trait locus affecting seed weight) to a simply controlled gene (seed colour) in common bean by
Sax (1923), it has taken more than 60 years for genetic markers to become a qualified tool for
plant breeding programs. In rice, the Xieyou 218 hybrid was the first to be developed through
MAS to select individuals carrying a bacterial blight-resistant gene. Marker-assisted selection
(MAS) can be applied at the seedling stage, with high precision and reductions in cost. Genetic
mapping of major genes and quantitative traits loci (QTLs) for agricultural traits is increasing
the integration of biotechnology with the conventional breeding process. Traits related to
disease resistance to pathogens and to the quality of some crop products are offering some
important examples of a possible routinary application of MAS. For more complex traits, like
yield and abiotic stress tolerance, a number of constraints have severe limitations on an efficient
utilization of MAS in plant breeding. However, the economic and biological constraints such
as a low return of investment in small-grain cereal breeding, lack of diagnostic markers, and
the prevalence of QTL-background effects hinder the broad implementation of MAS but over
the past 2 decades, a number of R-genes conferring resistance to a diverse range of pathogens
have been mapped in many crops using molecular markers.
Marker assisted selection is the breeding strategy in which selection for a gene is based on molecular markers closely linked to the gene of interest rather than the gene itself, and the markers are used to monitor the incorporation of the desirable allele from the donor source. Selection of a genotype carrying desirable gene via linked marker (s) is called Marker Assisted Selection. MAS can be applied to possible to use this kind of information.
The prerequisites for the classical procedure of MAS are the tight linkage between molecular marker and gene of interest and high heritability of the gene of interest. It is noteworthy that the “quality” and the number of markers have a major impact on the success of MAS. The quality of markers relates to their characteristics and to the cost and the efficiency of the genotyping process. The number of markers affects the reliability of the linkage between them and the gene(s). In other words, screening a large number of markers has the potential to identify close and reliable linkage between the marker and the gene of interest. MAS has greater potential for efficient gene pyramiding combining several important genes in one cultivar. MAS is gaining considerable importance as it can improve the efficiency of plant breeding through precise transfer of genomic regions of interest and acceleration of the recovery of the recurrent parent genome. Marker-assisted selection is gaining considerable importance as it would improve the efficiency of plant breeding through precise transfer of genomic regions of interest (foreground selection) and accelerating the recovery of the recurrent parent genome (background selection). The use of MAS in crop improvement will not only reduce the cost of developing new varieties but will also increase the precision and efficiency of selection in the breeding program as well as lessen the number of years required to come up with a new crop variety.
Marker Assisted Selection in Crop BreedingPawan Chauhan
Marker Assisted Selection is a value addition to conventional methods of Crop Breeding. It has been gaining importance in plant breeding with new generation of plant breeders and to get accurate and fast desired result from plant breeding.
Marker assisted selection or marker aided selection is an indirect selection process where a trait of interest is selected based on a marker linked to a trait of interest, rather than on the trait itself. This process has been extensively researched and proposed for plant and animal breeding.Marker-assisted breeding uses DNA markers associated with desirable traits to select a plant or animal for inclusion in a breeding program early in its development. ... This genetic test is helping breeders to select for hornless cattle, which makes it safer for the animals themselves and the people handling them.
I would like to share this presentation file.
Some basics information regarding to molecular plant breeding, hope this help the beginner who start working in this field.
Thanks for many original source of information (mainly from slideshare.net, IRRI, CIMMYT and any paper received from professor and some over the internet)
Strategies for mapping of genes for agronomic traits in plantstusharamodugu
The genomic regions associated with the expression of a quantitative trait is referred to as quantitative trait loci (QTL).
A QTL may contain one or more genes affecting the concerned quantitative trait.
Sax(1923) reported linkage between seed coat colour and seed size, which are qualitative and quantitative traits in common bean and the work highlighted the basic principles for mapping of polygenes based on the detection of association between a quantitative trait phenotype and a genetic marker.
Thoday (1961) explored this QTL concept further by combining elaborate cytogenetic techniques with genetic analysis to map QTLs for several quantitative traits in Drosophila
Molecular Markers: Indispensable Tools for Genetic Diversity Analysis and Cro...Premier Publishers
Recent progress in molecular biology has led to the development of new molecular tools that offer the promise of making plant breeding faster. Molecular markers are segments of DNA associated with agronomically important traits and can be used by plant breeders as selection tools. Breeders can use marker-assisted selection (MAS) to bypass the traditional phenotype-based selection methods in order to improve crop varieties with pyramiding the desirable traits within short time. Various molecular markers such as RAPD, SSR, ISSR, RFLP, AFLP, SNP, SCAR, CAPS, etc. are extensively used for plant genetic diversity studies and crop improvement biotechnology. These markers are different in characteristic properties, applicability to various plants, unique in the resolving power and also have own advantages and disadvantages. This review article provides a valuable insight into different molecular marker techniques, classification, their advantages, disadvantages, ways of actions, uses of molecular markers in plant genetic diversity analysis and quantitative trait loci (QTL) mapping. It could be helpful for plant scientists and breeders in MAS breeding and crop improvement biotechnology in the post-genomic era.
In this presentation, we will delve into the principles of QTL mapping and explore various strategies for mapping QTLs in plants. We will also discuss the advantages and limitations, and provide insights into how QTL mapping is advancing our understanding of genetics.
Role of Marker Assisted Selection in Plant Resistance RandeepChoudhary2
Topic Role of Marker Assisted Selection in Plant Resistance is described in detail including some case studies.
Types of markers used in genetic engineering and biotechnology are described in detail.
Marker assisted selection is a process whereby a marker (morphological, biochemical or one
based on DNA/RNA variation) is used for indirect selection of a genetic determinant of a trait
of interest. Since the first reported linkage of an agronomically important trait (a quantitative
trait locus affecting seed weight) to a simply controlled gene (seed colour) in common bean by
Sax (1923), it has taken more than 60 years for genetic markers to become a qualified tool for
plant breeding programs. In rice, the Xieyou 218 hybrid was the first to be developed through
MAS to select individuals carrying a bacterial blight-resistant gene. Marker-assisted selection
(MAS) can be applied at the seedling stage, with high precision and reductions in cost. Genetic
mapping of major genes and quantitative traits loci (QTLs) for agricultural traits is increasing
the integration of biotechnology with the conventional breeding process. Traits related to
disease resistance to pathogens and to the quality of some crop products are offering some
important examples of a possible routinary application of MAS. For more complex traits, like
yield and abiotic stress tolerance, a number of constraints have severe limitations on an efficient
utilization of MAS in plant breeding. However, the economic and biological constraints such
as a low return of investment in small-grain cereal breeding, lack of diagnostic markers, and
the prevalence of QTL-background effects hinder the broad implementation of MAS but over
the past 2 decades, a number of R-genes conferring resistance to a diverse range of pathogens
have been mapped in many crops using molecular markers.
Marker assisted selection is the breeding strategy in which selection for a gene is based on molecular markers closely linked to the gene of interest rather than the gene itself, and the markers are used to monitor the incorporation of the desirable allele from the donor source. Selection of a genotype carrying desirable gene via linked marker (s) is called Marker Assisted Selection. MAS can be applied to possible to use this kind of information.
The prerequisites for the classical procedure of MAS are the tight linkage between molecular marker and gene of interest and high heritability of the gene of interest. It is noteworthy that the “quality” and the number of markers have a major impact on the success of MAS. The quality of markers relates to their characteristics and to the cost and the efficiency of the genotyping process. The number of markers affects the reliability of the linkage between them and the gene(s). In other words, screening a large number of markers has the potential to identify close and reliable linkage between the marker and the gene of interest. MAS has greater potential for efficient gene pyramiding combining several important genes in one cultivar. MAS is gaining considerable importance as it can improve the efficiency of plant breeding through precise transfer of genomic regions of interest and acceleration of the recovery of the recurrent parent genome. Marker-assisted selection is gaining considerable importance as it would improve the efficiency of plant breeding through precise transfer of genomic regions of interest (foreground selection) and accelerating the recovery of the recurrent parent genome (background selection). The use of MAS in crop improvement will not only reduce the cost of developing new varieties but will also increase the precision and efficiency of selection in the breeding program as well as lessen the number of years required to come up with a new crop variety.
Marker Assisted Selection in Crop BreedingPawan Chauhan
Marker Assisted Selection is a value addition to conventional methods of Crop Breeding. It has been gaining importance in plant breeding with new generation of plant breeders and to get accurate and fast desired result from plant breeding.
Marker assisted selection or marker aided selection is an indirect selection process where a trait of interest is selected based on a marker linked to a trait of interest, rather than on the trait itself. This process has been extensively researched and proposed for plant and animal breeding.Marker-assisted breeding uses DNA markers associated with desirable traits to select a plant or animal for inclusion in a breeding program early in its development. ... This genetic test is helping breeders to select for hornless cattle, which makes it safer for the animals themselves and the people handling them.
I would like to share this presentation file.
Some basics information regarding to molecular plant breeding, hope this help the beginner who start working in this field.
Thanks for many original source of information (mainly from slideshare.net, IRRI, CIMMYT and any paper received from professor and some over the internet)
Strategies for mapping of genes for agronomic traits in plantstusharamodugu
The genomic regions associated with the expression of a quantitative trait is referred to as quantitative trait loci (QTL).
A QTL may contain one or more genes affecting the concerned quantitative trait.
Sax(1923) reported linkage between seed coat colour and seed size, which are qualitative and quantitative traits in common bean and the work highlighted the basic principles for mapping of polygenes based on the detection of association between a quantitative trait phenotype and a genetic marker.
Thoday (1961) explored this QTL concept further by combining elaborate cytogenetic techniques with genetic analysis to map QTLs for several quantitative traits in Drosophila
Molecular Markers: Indispensable Tools for Genetic Diversity Analysis and Cro...Premier Publishers
Recent progress in molecular biology has led to the development of new molecular tools that offer the promise of making plant breeding faster. Molecular markers are segments of DNA associated with agronomically important traits and can be used by plant breeders as selection tools. Breeders can use marker-assisted selection (MAS) to bypass the traditional phenotype-based selection methods in order to improve crop varieties with pyramiding the desirable traits within short time. Various molecular markers such as RAPD, SSR, ISSR, RFLP, AFLP, SNP, SCAR, CAPS, etc. are extensively used for plant genetic diversity studies and crop improvement biotechnology. These markers are different in characteristic properties, applicability to various plants, unique in the resolving power and also have own advantages and disadvantages. This review article provides a valuable insight into different molecular marker techniques, classification, their advantages, disadvantages, ways of actions, uses of molecular markers in plant genetic diversity analysis and quantitative trait loci (QTL) mapping. It could be helpful for plant scientists and breeders in MAS breeding and crop improvement biotechnology in the post-genomic era.
In this presentation, we will delve into the principles of QTL mapping and explore various strategies for mapping QTLs in plants. We will also discuss the advantages and limitations, and provide insights into how QTL mapping is advancing our understanding of genetics.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
Francesca Gottschalk - How can education support child empowerment.pptxEduSkills OECD
Francesca Gottschalk from the OECD’s Centre for Educational Research and Innovation presents at the Ask an Expert Webinar: How can education support child empowerment?
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
Introduction to AI for Nonprofits with Tapp NetworkTechSoup
Dive into the world of AI! Experts Jon Hill and Tareq Monaur will guide you through AI's role in enhancing nonprofit websites and basic marketing strategies, making it easy to understand and apply.
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
3. MARKER ASSISTED SELECTION
Marker assisted selection or marker aided selection (MAS) is an indirect selection process where a
trait of interest is selected based on a marker (morphological, biochemical or DNA/RNA variation)
linked to a trait of interest (e.g. productivity, disease resistance, abiotic stress tolerance, and
quality), rather than on the trait itself. This process has been extensively researched and proposed
for plant and animal breeding.
For example, using MAS to select individuals with disease resistance involves identifying a
marker allele that is linked with disease resistance rather than the level of disease resistance. The
assumption is that the marker associates at high frequency with the gene or quantitative trait
locus (QTL) of interest, due to genetic linkage (close proximity, on the chromosome, of the marker
locus and the disease resistance-determining locus). MAS can be useful to select for traits that are
difficult or expensive to measure, exhibit low heritability and/or are expressed late in development.
4. Marker types
The majority of MAS work in the present era uses DNA-based markers.However, the first markers
that allowed indirect selection of a trait of interest were morphological markers.
In 1923, Karl Sax first reported association of a simply inherited genetic marker with a quantitative
trait in plants when he observed segregation of seed size associated with segregation for a seed
coat color marker in beans (Phaseolus vulgaris L.).
In 1935, J. Rasmusson demonstrated linkage of flowering time (a quantitative trait) in peas with a
simply inherited gene for flower color.[7]
Markers may be:
Morphological – These were the first markers loci available that have an obvious impact on the
morphology of plants. These markers are often detectable by eye, by simple visual inspection.
Examples of this type of marker include the presence or absence of an awn, leaf sheath coloration,
height, grain color, aroma of rice etc. In well-characterized crops like maize, tomato, pea, barley
or wheat, tens or hundreds of genes that determine morphological traits have been mapped to
specific chromosome locations.
Biochemical – A protein that can be extracted and observed; for example, isozymes and storage
proteins.
5. • Cytological – Cytological markers are chromosomal features that can be identified through
microscopy. These generally take the form of chromosome bands, regions of chromatin that
become impregnated with specific dyes used in cytology. The presence or absence of a
chromosome band can be correlated with a particular trait, indicating that the locus responsible
for the trait is located within or near (tightly linked) to the banded region.
• Morphological and cytological markers formed the backbone of early genetic studies in crops such
as wheat and maize.
• DNA-based- Including microsatellites (also known as short tandem repeats, STRs, or simple
sequence repeats, SSRs), restriction fragment length polymorphism (RFLP), random amplification
of polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and single
nucleotide polymorphisms (SNPs)
6. Important properties of ideal markers for MAS
• An ideal marker: Has easy recognition of phenotypes - ideally all possible phenotypes (homo-
and heterozygotes) from all possible alleles
• Demonstrates measurable differences in expression between trait types or gene of interest
alleles, early in the development of the organism
• Testing for the marker does not have variable success depending on the allele at the marker
locus or the allele at the target locus (the gene of interest that determines the trait of interest).
• Low or null interaction among the markers allowing the use of many at the same time in a
segregating population
• Abundant in number
• Polymorphic
7. Steps for MAS
Generally the first step is to map the gene or quantitative trait locus (QTL) of interest first
by using different techniques and then using this information for marker assisted selection.
Generally, the markers to be used should be close to gene of interest (<5 recombination
unit or cM) in order to ensure that only minor fraction of the selected individuals will be
recombinants. Generally, not only a single marker but rather two markers are used in
order to reduce the chances of an error due to homologous recombination. For example, if
two flanking markers are used at same time with an interval between them of
approximately 20cM, there is higher probability (99%) for recovery of the target gene.
QTL mapping techniques.
Quantitative_trait_locus § QTL_mapping
In plants QTL mapping is generally achieved using bi-parental cross populations; a cross
between two parents which have a contrasting phenotype for the trait of interest are
developed. Commonly used populations are near isogenic lines (NILs), recombinant
inbred lines (RILs), doubled haploids (DH), back cross and F2. Linkage between the
phenotype and markers which have already been mapped is tested in these populations in
order to determine the position of the QTL. Such techniques are based on linkage and are
therefore referred to as "linkage mapping".