The document summarizes the research of Dr. Thomas Hudson Sanderson and his team on using non-invasive light therapy to modulate mitochondria and reduce reactive oxygen species production after brain ischemia. They have found specific infrared light wavelengths that can inhibit cytochrome c oxidase activity and mitochondrial respiration in cellular and animal models of brain injury. Further pre-clinical studies in a pig model of cardiac arrest showed inhibitory light therapy reduced neuronal injury and improved functional outcomes. Based on this research, Dr. Sanderson co-founded Mitovation, Inc. to develop a medical device for treating post-ischemic brain injury in humans using non-invasive mitochondrial modulation therapy.
As more breakthroughs in the understanding of photomedicine and low level laser therapy occur, it is increasingly important for physicians to understand the role of low level laser therapy in the management of androgenetic alopecia (male and female pattern hair loss). This brief overview from the 19th Annual World Congress of Anti-Aging and Aesthetic medicine (A4M) in Orlando April 2011 followed Harvard/MIT professor, Dr. Michael Hamblin's detailed talk on the mechanisms of low level laser therapy.
As more breakthroughs in the understanding of photomedicine and low level laser therapy occur, it is increasingly important for physicians to understand the role of low level laser therapy in the management of androgenetic alopecia (male and female pattern hair loss). This brief overview from the 19th Annual World Congress of Anti-Aging and Aesthetic medicine (A4M) in Orlando April 2011 followed Harvard/MIT professor, Dr. Michael Hamblin's detailed talk on the mechanisms of low level laser therapy.
PRP Platelet Rich Plasma is a non-surgical, non-invasive treatment for hair loss. “Cell therapy” is a relatively new frontier in medical technology which involves introducing cells into a tissue in order to treat a disease or condition. Areas of research such as stem cell processing and activation, cell multiplication, cytokines, growth factors, extracellular matrix products, bioscaffolds, platelet rich plasma (PRP), gene therapy, tissue repair and more are some of the exciting new ways in which scientists are investigating in the use of cell therapy to physicians improve outcomes and treat a variety of conditions.
Adjunctive therapies which impact wound healing like the use of hyperbaric oxygen, copper peptide solutions, phototherapy, extracellular matrix (ECM), protein soaks, etc. have been a part of Dr. Alan Bauman’s post-operative treatment regimens after hair transplantation for years. Helping the body safely “help itself” is the cornerstone of these types of treatments which often utilize natural components found in the human body. Along those lines, PRP has already been found to be a helpful adjunct successfully in use in many areas of medicine, including orthopedics, dentistry and plastic surgery for tissue repair, tissue regeneration and wound healing.
What is Platelet Rich Plasma (PRP)?
Produced in just minutes from a small quantity of your own blood, PRP contains a wide variety of the body’s own natural growth factors which may be helpful in accelerating tissue regeneration, improving healing time and stimulating hair growth. Platelets are cell fragments that are normally found in the blood and play a key role in blood clotting and are a natural source of growth factors. Special medical equipment is used by your physician to separate a platelets from a small sample of your own blood and prepare them for use. Once applied and activated, platelets release their stores of powerful growth factors and other molecules.
What Growth Factors are found in Platelet Rich Plasma (PRP)? The platelets collected in PRP are activated by thrombin and calcium chloride to induce the release of these growth factor proteins and other cytokines:
• platelet-derived growth factor
• transforming growth factor beta
• fibroblast growth factor
• insulin-like growth factor 1
• insulin-like growth factor 2
• vascular endothelial growth factor
• epidermal growth factor
• interleukin-8
• keratinocyte growth factor
• connective tissue growth factor
HOW IS PRP MADE?
PRP is made from a small sample of your own blood. The FDA-approved process concentrates the platelets found in the blood sample and separates them from other blood components. These concentrated platelets contain huge reservoirs of growth factors and wound healing factors. Growth factors are natural components of your body whose potential benefits can now be harnessed using modern medical technology. For hands-on PRP training classes v
Journal of Stem Cells Research, Reviews & Reports is a peer-reviewed, open access journal published by Austin Publishers. It provides easy access to high quality Manuscripts in all related aspects covering Stem cell research that focuses on stem cells, which have a capacity to regenerate and develop into other types of cells namely, like kidney cells, liver cells, heart cells, etc. These circulate and function to replace dysfunctional cells, naturally maintaining optimal health. The Journal encourages all the current medical research that is focused on two particular types of stem cells -- adult and embryonic stem cells that are used in various stem cell therapies against many dreadful diseases.
Austin Publishing Group is a successful host of more than hundred peer reviewed, open access journals in various fields of science and medicine with intent to bridge the gap between academia and research access.
Journal of Stem Cells Research, Reviews & Reports accepts original research articles, review articles, case reports, mini reviews, rapid communication, opinions and editorials on all the related aspects of Stem Cells and Cell-Based Therapies.
A branch of medicine dealing with diseases and metabolic disorders that affect mitochondria. Focusing on diagnosing and treatment of wide range of these diseases. The symptom of these diseases varies from metabolic-induced developmental delay to complex problems that involve many body systems.
Magnets - Not Drugs: TMS IMMH San Antonio 2014Louis Cady, MD
In this talk, Dr. Cady covers a remarkable new treatment for depression: transcranial magnetic stimulation. The historical roots of this treatment are traced, followed by a review of the literature in terms of the proven efficacy of this treatment. A comparison with ECT shows that TMS has a very favorable profile, with remarkably fewer side effects and incredibly better tolerated side effects compared to ECT. Given that this was a "CME" talk, off-label uses of TMS were reviewed, including stepping stones for future avenues to explore
In Feb 2022, Dr. Cassano presented this NYU-MGH study at LifeStance Health. The presentation covers in-vitro and pre-clinical data in support of the use of transcranial PBM for Alzheimer’s disease (AD). The most recent clinical data on PBM for AD were also reviewed. The NYU-MGH study was described: a large randomized, double-blind clinical trial (n=125) sponsored by NIH/NIA and by the Alzheimer’s Association.
A novel implantable dual microelectrode for monitoring/predicting post trauma...dharmakarma
Here, we describe a novel dual microelectrode concept based on brain oxygenation that can be used to predict seizures caused due to traumatic brain injury. Since brain oxygenation occurs slightly prior to chaotic neural firing, it can be used to predict in advance the occurrence of a seizure.
Biology, genetics, nanotechnology, neuroscience, materials science, biotech, ...Brian Russell
Over the past two years I've done a lot of interesting research which I've decided to aggregate. My research pertains to the following: Biology, Genetics, Nanotechnology, Neuroscience, Materials Science, Biotechnology, Chemical Engineering, All Things 3-D, Super Computing, Quantum Physics, Energy, Design, & Sustainability.
NanoTechnology in the Field of Medicine.pptAkshitRajput25
NanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Fie
a presentation on experimental study design for CVD models, will help you in selecting the case to study and pick a right one. as Cardiovascular plays a important role.
PRP Platelet Rich Plasma is a non-surgical, non-invasive treatment for hair loss. “Cell therapy” is a relatively new frontier in medical technology which involves introducing cells into a tissue in order to treat a disease or condition. Areas of research such as stem cell processing and activation, cell multiplication, cytokines, growth factors, extracellular matrix products, bioscaffolds, platelet rich plasma (PRP), gene therapy, tissue repair and more are some of the exciting new ways in which scientists are investigating in the use of cell therapy to physicians improve outcomes and treat a variety of conditions.
Adjunctive therapies which impact wound healing like the use of hyperbaric oxygen, copper peptide solutions, phototherapy, extracellular matrix (ECM), protein soaks, etc. have been a part of Dr. Alan Bauman’s post-operative treatment regimens after hair transplantation for years. Helping the body safely “help itself” is the cornerstone of these types of treatments which often utilize natural components found in the human body. Along those lines, PRP has already been found to be a helpful adjunct successfully in use in many areas of medicine, including orthopedics, dentistry and plastic surgery for tissue repair, tissue regeneration and wound healing.
What is Platelet Rich Plasma (PRP)?
Produced in just minutes from a small quantity of your own blood, PRP contains a wide variety of the body’s own natural growth factors which may be helpful in accelerating tissue regeneration, improving healing time and stimulating hair growth. Platelets are cell fragments that are normally found in the blood and play a key role in blood clotting and are a natural source of growth factors. Special medical equipment is used by your physician to separate a platelets from a small sample of your own blood and prepare them for use. Once applied and activated, platelets release their stores of powerful growth factors and other molecules.
What Growth Factors are found in Platelet Rich Plasma (PRP)? The platelets collected in PRP are activated by thrombin and calcium chloride to induce the release of these growth factor proteins and other cytokines:
• platelet-derived growth factor
• transforming growth factor beta
• fibroblast growth factor
• insulin-like growth factor 1
• insulin-like growth factor 2
• vascular endothelial growth factor
• epidermal growth factor
• interleukin-8
• keratinocyte growth factor
• connective tissue growth factor
HOW IS PRP MADE?
PRP is made from a small sample of your own blood. The FDA-approved process concentrates the platelets found in the blood sample and separates them from other blood components. These concentrated platelets contain huge reservoirs of growth factors and wound healing factors. Growth factors are natural components of your body whose potential benefits can now be harnessed using modern medical technology. For hands-on PRP training classes v
Journal of Stem Cells Research, Reviews & Reports is a peer-reviewed, open access journal published by Austin Publishers. It provides easy access to high quality Manuscripts in all related aspects covering Stem cell research that focuses on stem cells, which have a capacity to regenerate and develop into other types of cells namely, like kidney cells, liver cells, heart cells, etc. These circulate and function to replace dysfunctional cells, naturally maintaining optimal health. The Journal encourages all the current medical research that is focused on two particular types of stem cells -- adult and embryonic stem cells that are used in various stem cell therapies against many dreadful diseases.
Austin Publishing Group is a successful host of more than hundred peer reviewed, open access journals in various fields of science and medicine with intent to bridge the gap between academia and research access.
Journal of Stem Cells Research, Reviews & Reports accepts original research articles, review articles, case reports, mini reviews, rapid communication, opinions and editorials on all the related aspects of Stem Cells and Cell-Based Therapies.
A branch of medicine dealing with diseases and metabolic disorders that affect mitochondria. Focusing on diagnosing and treatment of wide range of these diseases. The symptom of these diseases varies from metabolic-induced developmental delay to complex problems that involve many body systems.
Magnets - Not Drugs: TMS IMMH San Antonio 2014Louis Cady, MD
In this talk, Dr. Cady covers a remarkable new treatment for depression: transcranial magnetic stimulation. The historical roots of this treatment are traced, followed by a review of the literature in terms of the proven efficacy of this treatment. A comparison with ECT shows that TMS has a very favorable profile, with remarkably fewer side effects and incredibly better tolerated side effects compared to ECT. Given that this was a "CME" talk, off-label uses of TMS were reviewed, including stepping stones for future avenues to explore
In Feb 2022, Dr. Cassano presented this NYU-MGH study at LifeStance Health. The presentation covers in-vitro and pre-clinical data in support of the use of transcranial PBM for Alzheimer’s disease (AD). The most recent clinical data on PBM for AD were also reviewed. The NYU-MGH study was described: a large randomized, double-blind clinical trial (n=125) sponsored by NIH/NIA and by the Alzheimer’s Association.
A novel implantable dual microelectrode for monitoring/predicting post trauma...dharmakarma
Here, we describe a novel dual microelectrode concept based on brain oxygenation that can be used to predict seizures caused due to traumatic brain injury. Since brain oxygenation occurs slightly prior to chaotic neural firing, it can be used to predict in advance the occurrence of a seizure.
Biology, genetics, nanotechnology, neuroscience, materials science, biotech, ...Brian Russell
Over the past two years I've done a lot of interesting research which I've decided to aggregate. My research pertains to the following: Biology, Genetics, Nanotechnology, Neuroscience, Materials Science, Biotechnology, Chemical Engineering, All Things 3-D, Super Computing, Quantum Physics, Energy, Design, & Sustainability.
NanoTechnology in the Field of Medicine.pptAkshitRajput25
NanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Field of MediNanoTechnology in the Fie
a presentation on experimental study design for CVD models, will help you in selecting the case to study and pick a right one. as Cardiovascular plays a important role.
differentiating different brain lesion using MR Spectroscopy which is a newer MR technique to quantify different metabolites present within the lesion and adjacent parenchyma
Protection of humans during long space flight. using cannabis to reduce biol...Dmitri Popov
Protection of humans during long space flight. using cannabis to reduce biological consequences of high doses of radiation, treat stress, anxiety, and depression Associated with Long-term Space Flight to Mars.
Determine the frequency of 16 high-risk conditions and associated complications in a Michigan state-wide network of academic and community EDs (MiPEM) during the COVID-19 pandemic
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Integrating Ayurveda into Parkinson’s Management: A Holistic ApproachAyurveda ForAll
Explore the benefits of combining Ayurveda with conventional Parkinson's treatments. Learn how a holistic approach can manage symptoms, enhance well-being, and balance body energies. Discover the steps to safely integrate Ayurvedic practices into your Parkinson’s care plan, including expert guidance on diet, herbal remedies, and lifestyle modifications.
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
Adv. biopharm. APPLICATION OF PHARMACOKINETICS : TARGETED DRUG DELIVERY SYSTEMSAkankshaAshtankar
MIP 201T & MPH 202T
ADVANCED BIOPHARMACEUTICS & PHARMACOKINETICS : UNIT 5
APPLICATION OF PHARMACOKINETICS : TARGETED DRUG DELIVERY SYSTEMS By - AKANKSHA ASHTANKAR
ABDOMINAL TRAUMA in pediatrics part one.drhasanrajab
Abdominal trauma in pediatrics refers to injuries or damage to the abdominal organs in children. It can occur due to various causes such as falls, motor vehicle accidents, sports-related injuries, and physical abuse. Children are more vulnerable to abdominal trauma due to their unique anatomical and physiological characteristics. Signs and symptoms include abdominal pain, tenderness, distension, vomiting, and signs of shock. Diagnosis involves physical examination, imaging studies, and laboratory tests. Management depends on the severity and may involve conservative treatment or surgical intervention. Prevention is crucial in reducing the incidence of abdominal trauma in children.
263778731218 Abortion Clinic /Pills In Harare ,sisternakatoto
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Rasamanikya is a excellent preparation in the field of Rasashastra, it is used in various Kushtha Roga, Shwasa, Vicharchika, Bhagandara, Vatarakta, and Phiranga Roga. In this article Preparation& Comparative analytical profile for both Formulationon i.e Rasamanikya prepared by Kushmanda swarasa & Churnodhaka Shodita Haratala. The study aims to provide insights into the comparative efficacy and analytical aspects of these formulations for enhanced therapeutic outcomes.
Basavarajeeyam is a Sreshta Sangraha grantha (Compiled book ), written by Neelkanta kotturu Basavaraja Virachita. It contains 25 Prakaranas, First 24 Chapters related to Rogas& 25th to Rasadravyas.
Here is the updated list of Top Best Ayurvedic medicine for Gas and Indigestion and those are Gas-O-Go Syp for Dyspepsia | Lavizyme Syrup for Acidity | Yumzyme Hepatoprotective Capsules etc
Mitochondria in Post-Ischemic Brain Injury: Mechanistic Insights and Novel Therapeutics by Tom Sanderson, PhD
1. Mitochondria in Post-Ischemic Brain Injury:
Mechanistic Insights and Novel Therapeutics
Thomas Hudson Sanderson, Ph.D.
Associate Professor
Departments of Emergency Medicine and Molecular and Integrative Physiology
University of Michigan Medical School, Ann Arbor MI
Grant/Research Support
NIH - NINDS R42NS105238
NIH - NINDS R01NS091242
NIH - NINDS R01NS076715
DoD - W81XWH-16-0175
Kellogg Foundation
NEI/MTRAC
American Heart Association
Disclosures
Patents
8945196- “Light Therapy Treatment”
9610460- “Light Therapy Treatment –Method”
Company Interests
Co-Founder and CSO of Mitovation, Inc.
2. Understanding Brain Ischemia: From Biochemistry to the Pig
Molecular Interrogation
• Cellular models – primary neurons or immortalized cells
• Genetic manipulation – viral constructs, plasmids, Cas9/CRISPR,
transgenic mice
Small Animal Models of Brain Ischemia
• Cardiac arrest/resuscitation – mouse
• Adult global brain ischemia – rat: 2VOH
• Stroke - focal ischemia – rat: MCAO
• Perinatal hypoxia/ischemia – rat: Vannucci model of HIE
Large Animal Translational Models
• Pig model of cardiac arrest/resuscitation
• Piglet model of perinatal hypoxia/asphyxia
Sanderson Lab: Basic and Translational Neuroscience
EMERGENCY MEDICINE
3. Non-Invasive Mitochondrial Modulation Therapy
• Therapeutic discovery→ development → translation
• Isolated enzyme studies on CcO activity
• Cellular, mechanistic, and efficacy investigations
• Pre-clinical studies: cardiac arrest/resuscitation in pigs.
Overview
EMERGENCY MEDICINE
4. Design: Utilize a combined O2
electrode/ spectrophotometer to
find IRL wavelengths that modulate
CcO and mitochondrial respiration
Mechanistic Discovery: The Foundation
EMERGENCY MEDICINE
5. Design: Utilize a combined O2
electrode/ spectrophotometer to
find IRL wavelengths that modulate
CcO and mitochondrial respiration
• Two wavelengths of IRL, 750nm
and 950nm, were identified that
inhibited O2 consumption
(attenuated CcO activity).
• Effect translates to intact
mitochondria.
• 810nm (used in previous
studies) activates CcO.
Mechanistic Discovery: The Foundation
EMERGENCY MEDICINE
6. How Does IRL Modulate Mitochondrial Respiration?
EMERGENCY MEDICINE
It is the scientific consensus that
mitochondrial CcO is the primary
cellular photo-acceptor of IRL.
CcO contains two copper centers:
CuA and CuB (blue and green
arrows)
• Involved in enzyme catalysis and
have been shown to function as
the photo-acceptors for IRL
7. Problem: Generation of ROS during early reperfusion precipitates
significant neuronal injury and cell death.
Barriers to Previous Therapeutic Approaches:
• ROS act rapidly (nano to milliseconds) and irreversibly.
• Scavenging compounds must be at the appropriate subcellular
targets at the necessary concentration at the time of reflow.
• Pretreatment needed to deliver compounds to ischemic tissue.
Solution: Non-Invasive Mitochondrial Modulation.
Hypothesis: Non-Invasive mitochondrial modulation therapy can
overcome these barriers to ROS therapy and reduce post-ischemic
brain injury.
Potential for Therapeutic Use
EMERGENCY MEDICINE
8. Balance Between Energy and ROS
EMERGENCY MEDICINE
• The mitochondrial
membrane potential (m)
is critical for energy
production (ATP) and
reactive oxygen species
(ROS) generation.
• Dynamic control of
mitochondrial membrane
potential is disrupted during
ischemia/reperfusion.
9. Balance Between Energy and ROS
EMERGENCY MEDICINE
• The mitochondrial
membrane potential (m)
is critical for energy
production (ATP) and
reactive oxygen species
(ROS) generation.
• Dynamic control of
mitochondrial membrane
potential is disrupted during
ischemia/reperfusion.
During ischemia mitochondrial
membrane potential depolarizes
10. Balance Between Energy and ROS
EMERGENCY MEDICINE
• The mitochondrial
membrane potential (m)
is critical for energy
production (ATP) and
reactive oxygen species
(ROS) generation.
• Dynamic control of
mitochondrial membrane
potential is disrupted during
ischemia/reperfusion.
During reperfusion mitochondrial
membrane potential is rapidly restored
11. Balance Between Energy and ROS
EMERGENCY MEDICINE
• The mitochondrial
membrane potential (m)
is critical for energy
production (ATP) and
reactive oxygen species
(ROS) generation.
• Dynamic control of
mitochondrial membrane
potential is disrupted during
ischemia/reperfusion.
During reperfusion
increased mitochondrial
membrane potential
levelslead to excessive
ROS production.
During reperfusion mitochondrial
membrane potential is rapidly restored
12. Balance Between Energy and ROS
EMERGENCY MEDICINE
Mild mitochondrial modulation
can provide significant
reduction in ROS production.
• The mitochondrial
membrane potential (m)
is critical for energy
production (ATP) and
reactive oxygen species
(ROS) generation.
• Dynamic control of
mitochondrial membrane
potential is disrupted during
ischemia/reperfusion.
During reperfusion
increased mitochondrial
membrane potential
levelslead to excessive
ROS production.
13. In Vitro – Mitochondrial ROS and Cell Death
EMERGENCY MEDICINE
Glutamate Exposure:
• Glutamate promotes
mitochondrial ROS
generation (Red).
• IRL treatment minimized
mitochondrial ROS.
Oxygen Glucose
Deprivation (OGD):
• OGD causes loss of live
neurons (green) and
increases dead neurons
(red).
• Inhibitory IRL increases
neuronal survival.
• Excitatory IRL is ineffective.
In Vitro Models of Neuronal Injury
Sanderson TH, Hüttemann M. et al. Sci Rep 2018.
14. Efficacy Trials in Small Animal Model
EMERGENCY MEDICINE
Sanderson TH, Hüttemann M. et al. Sci Rep 2018.
Global Brain Ischemia Protocol
• Random enrollment, blinded treatment
and analysis.
• IRL treatment with LED diodes for a 2
hour duration.
• No increase in brain temperature.
Brain Injury with Histology
• Global brain ischemia caused loss of
neurons in the hippocampus.
• Inhibitory IRL prevented neuronal injury.
• Excitatory IRL was ineffective.
• Functional improvement with treatment
detected with RAM.
• Delayed treatment effective, but efficacy
reduced.
15. Pre-Clinical Evaluation of Mitovation Technology
EMERGENCY MEDICINE
Pig Cardiac Arrest/Resuscitation – Survival Studies
• Random enrollment, blinded treatment and analysis.
• IRL treatment with bench prototype - LED diodes for a 2 hour
duration. Device provides light penetration through pig brain.
Post-Ischemic Brain Injury. Cardiac arrest/resuscitation
resulted in loss of neurons in the hippocampus 4 days after
ROSC.
• Inhibitory IRL reduced neuronal injury.
• Early evidence of functional improvement.
16. Mitovation, Inc. is a medical device company
developing a non-invasive platform technology
for the treatment of post-ischemic brain injury.
• A start-up company founded by:
Thomas H. Sanderson, Ph.D.
Maik Hüttemann, Ph.D.
Mark Morsfield, M.B.A.
The Mitovation Device:
• Disposable human interface - Fiber optic
light delivery system.
• Therapeutic light generator - Compact,
portable, and powered by a battery or
electrical outlet.
MTRAC and the DoD funded the alpha
prototype design through Tekna, Inc.
• Regulatory and efficacy testing ongoing
through Mitovation, Inc. and the
Sanderson lab.
Human Device Prototyping
EMERGENCY MEDICINE Mitovation, Inc.
17. Research Teams
SANDERSON LAB
Kathleen Maheras, PhD
Postdoctoral Fellow - Genetics, molecular
biology, mouse cardiac/arrest resuscitation
Joseph Wider, PhD
Postdoctoral Fellow - Large/small animal
models of brain and heart ischemia, cell
culture/biochemistry
Erin Gruley, BS
Research Specialist - Large animal
surgery, porcine behavioral studies,
neonatal brain injury
Sarita Raghunayakula, MS
Research Specialist - Molecular biology,
cell culture, primary neuron
isolation/culturing, genetic manipulation
Anthony Anzell, BS
PhD candidate - Primary neuron culture, in
vitro hypoxia, live cell imaging
Christos Strubakos, MS
PhD candidate - Ischemic stroke, MRI
EMERGENCY
MITOVATION, INC
Thomas Sanderson, PhD
Co-Founder and Chief Scientific Officer,
Mitovation, Inc.
Maik Hüttemann, PhD
Co-Founder and Chief Technology
Officer, Mitovation, Inc.
Professor of Molecular Medicine, Wayne
State University
Mark Morsfield, MBA
Chief Executive Officer, Mitovation, Inc.
Christian Reynolds, PhD
Director of Translational Research,
Mitovation, Inc. Assistant Professor,
Wayne State University
Joseph Wider, PhD
Research Fellow, University of Michigan
Lola Tatum
Grants Management Specialist,
Controller, IBT
Kimberly Johnston, DVM
Veterinary Surgeon, IBT