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Dr. Dina A. B. Awad
Lecturer food hygiene and control , faculty of veterinary medicine
Benha university, Egypt
Milk thermal treatments
1- Pasteurization 2- Sterilization
3- Boiling
Chemical, organoleptic properties of milk
???????????????????????????????????
1- Pasteurization processes
Raw milk
Certain
temperature
Certain
time
Sudden cooling
Minimizing
pathogenic organisms
Aim Associated
Minimal chemical,
physical changes
63°C/30 min
72°C/15sec
85°C/2-4 sec
4-10° C
Receiving of raw milk at 4 °C
Organoleptic examination
Acidity determination (C.O.B,A.P.T)
Dirt & sediment examination
Antibiotic residues & T.B.C & MBRT
Filtration 35-50 °C OR clarification 4.5 °C
Standardization
Homogenization
Pearson square
(add cream or skim milk)
(LTLT) 63C/30min
Kill 98% bacteria
Not affect chemical properties
1- avoid cream line
2-mouth feel
3- improve color
Pasteurization process
(HTST) 72C/ 15 sec.
Kill 99% bacteria
Slightly affect chemical properties
(Flash method) 85C/ 2-4 sec.
Kill 99.5% bacteria
Slightly affect chemical properties
Pasteurization
flow chart
Cooling less 7°C (inhibit thermoduric bacteria)&packaging (7 days)
Coxiella
burnteii
Milk
receiving
clarification 4.5 °C
Milk mechanical clarifier
Filtration 35-50 °C
Applied under centrifugation
(ppt dirt) Producing clarifier slime
Remove dust, somatic cell,some bacteria
Using disc wool or synthetic filters
Remove visible dirt and sediment,
coarse particles
But not the bacterial cells
Standardization
10
1st stage
2nd stage
Advantages:
1- Smaller fat globules --no cream-line formation
2-Whiter and more appetizing colour.
3-More better mouthfeel.
4-Better stability of cultured milk products.
Disadvantages:
1-May increased sensitivity to light – sunlight can
result in “sunlight flavour” .
2-More susceptible for rancid flavour.
April /2020
Dr.Dina A. B.Awad
Homogenization
Factors affecting keeping quality of pasteurized milk
Initial
bacterial
count
Efficiency of
pasteurization
Process
Sudden
cooling at
5-7 C
Post
pasteurization
contamination
Time –temperature factor (accurate) (phosphatase enz.)
To stop the action of bacteria that resist pasteurized temperature.
Insufficient clean piplines,tanks,filling machine, contamination water supply, poor personal hygiene
To avoid that??????????????????????????????????????????????????????????????????????????????
- Thermophilic bacteria-- resist holding method but HTST destroy it. St. coccus thermophilus
Thermoduric bacteria– resist pasteurization (spore former) as bacillus cereus,bacillus subtilus. (bactofugation)
Pschrophilic bacteria –as pseudomoans bacteria produce heat resistant enzymespoilage in pasteurized milk.(thermisation)
Spoilage of pasteurized milk
When stored at high temperature or at room temperature or kept opened – shelf life
Growth thermophilc bacteria
5.5pH Growth of lactobacilli
4 or less pH
N.B
Survive thermoduric bacteria,
bacillus (Sweet curdling)
(Rennin like enz.)
Mould &yeast pH
--Their presence indicate post
pasteurization contamination
Extend shelf life (ESL)--- means extended shelf life of the product
With maintaining its nutritional value (ultra-pasteurization )
125-130°C –2-4 sec
Milk sterilization (long
life milk)
A process in which milk is heated to a certain time that all microorganisms are destroyed
Producing high quality milk could stored for long time at room temperature (tropical areas).
Methods of
sterilization
In –bottle (container) UHT(uprization)
Sterilization for both milk &bottle
Flow chart for in -bottle sterilization
APT Test negative
Remove debris(brown sediment??)
Avoid cream line formation
Initial removal bacterial spores
Pre-serilization of milk at 80 C
Bottling in pre-wormed bottles
Milk in bottle sterilization
Cooling gradual within 1 hr
At 115-120/15-20 min in steam
Kept at room temp. 6 months
Page 132
Flow chart UHT sterilization
APT Test negative
Remove debris(brown sediment??)
Direct heating type
Inject milk in steam
Fearing from unsterile packaging
UHT process 140 – 150° C/1-4 sec.
Homogenization (avoid aggregates)
Packaging under O3
Page 132
Tubular heating using
Plate heat exchanger
Steam instead of hot water
Advantage and disadvantages UHT sterilization
Milk is effectively sterilized Gelation, ppt protein, loss vit.,cooked
Spoilage of UHT sterilization
Page 130
Rare:::::::::but,,,,,,,,,,,,,,,,,,heat resistant spores ---
survived Post- heat treatment
contamination
Psychrotrophic
organisms
Dual heat treatment
140C/5 sec
60 C/5 min
Bactofugation
Milk thermal treatment

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Milk thermal treatment

  • 1. Dr. Dina A. B. Awad Lecturer food hygiene and control , faculty of veterinary medicine Benha university, Egypt
  • 2. Milk thermal treatments 1- Pasteurization 2- Sterilization 3- Boiling Chemical, organoleptic properties of milk ???????????????????????????????????
  • 3. 1- Pasteurization processes Raw milk Certain temperature Certain time Sudden cooling Minimizing pathogenic organisms Aim Associated Minimal chemical, physical changes 63°C/30 min 72°C/15sec 85°C/2-4 sec 4-10° C
  • 4. Receiving of raw milk at 4 °C Organoleptic examination Acidity determination (C.O.B,A.P.T) Dirt & sediment examination Antibiotic residues & T.B.C & MBRT Filtration 35-50 °C OR clarification 4.5 °C Standardization Homogenization Pearson square (add cream or skim milk) (LTLT) 63C/30min Kill 98% bacteria Not affect chemical properties 1- avoid cream line 2-mouth feel 3- improve color Pasteurization process (HTST) 72C/ 15 sec. Kill 99% bacteria Slightly affect chemical properties (Flash method) 85C/ 2-4 sec. Kill 99.5% bacteria Slightly affect chemical properties Pasteurization flow chart Cooling less 7°C (inhibit thermoduric bacteria)&packaging (7 days) Coxiella burnteii
  • 5.
  • 6.
  • 8. clarification 4.5 °C Milk mechanical clarifier Filtration 35-50 °C Applied under centrifugation (ppt dirt) Producing clarifier slime Remove dust, somatic cell,some bacteria Using disc wool or synthetic filters Remove visible dirt and sediment, coarse particles But not the bacterial cells
  • 10. 10 1st stage 2nd stage Advantages: 1- Smaller fat globules --no cream-line formation 2-Whiter and more appetizing colour. 3-More better mouthfeel. 4-Better stability of cultured milk products. Disadvantages: 1-May increased sensitivity to light – sunlight can result in “sunlight flavour” . 2-More susceptible for rancid flavour. April /2020 Dr.Dina A. B.Awad Homogenization
  • 11. Factors affecting keeping quality of pasteurized milk Initial bacterial count Efficiency of pasteurization Process Sudden cooling at 5-7 C Post pasteurization contamination Time –temperature factor (accurate) (phosphatase enz.) To stop the action of bacteria that resist pasteurized temperature. Insufficient clean piplines,tanks,filling machine, contamination water supply, poor personal hygiene To avoid that?????????????????????????????????????????????????????????????????????????????? - Thermophilic bacteria-- resist holding method but HTST destroy it. St. coccus thermophilus Thermoduric bacteria– resist pasteurization (spore former) as bacillus cereus,bacillus subtilus. (bactofugation) Pschrophilic bacteria –as pseudomoans bacteria produce heat resistant enzymespoilage in pasteurized milk.(thermisation)
  • 12. Spoilage of pasteurized milk When stored at high temperature or at room temperature or kept opened – shelf life Growth thermophilc bacteria 5.5pH Growth of lactobacilli 4 or less pH N.B Survive thermoduric bacteria, bacillus (Sweet curdling) (Rennin like enz.) Mould &yeast pH --Their presence indicate post pasteurization contamination Extend shelf life (ESL)--- means extended shelf life of the product With maintaining its nutritional value (ultra-pasteurization ) 125-130°C –2-4 sec
  • 13. Milk sterilization (long life milk) A process in which milk is heated to a certain time that all microorganisms are destroyed Producing high quality milk could stored for long time at room temperature (tropical areas). Methods of sterilization In –bottle (container) UHT(uprization) Sterilization for both milk &bottle
  • 14. Flow chart for in -bottle sterilization APT Test negative Remove debris(brown sediment??) Avoid cream line formation Initial removal bacterial spores Pre-serilization of milk at 80 C Bottling in pre-wormed bottles Milk in bottle sterilization Cooling gradual within 1 hr At 115-120/15-20 min in steam Kept at room temp. 6 months Page 132
  • 15. Flow chart UHT sterilization APT Test negative Remove debris(brown sediment??) Direct heating type Inject milk in steam Fearing from unsterile packaging UHT process 140 – 150° C/1-4 sec. Homogenization (avoid aggregates) Packaging under O3 Page 132 Tubular heating using Plate heat exchanger Steam instead of hot water
  • 16. Advantage and disadvantages UHT sterilization Milk is effectively sterilized Gelation, ppt protein, loss vit.,cooked Spoilage of UHT sterilization Page 130 Rare:::::::::but,,,,,,,,,,,,,,,,,,heat resistant spores --- survived Post- heat treatment contamination Psychrotrophic organisms Dual heat treatment 140C/5 sec 60 C/5 min Bactofugation