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Microscopy of Kurchi bark
Mr. Vipul. A. Sansare
M.Pharm (Pharmaceutics)
Contact No. :8692930764
Email :avipulsansare@gmail.com
Synonym: Holarrhena, Kurchi
BS: Dried stem bark of the
Holarrhena antidysentrica
Family: Apocynaceae.
Collected from plants of 8 to 12 years old.
Chemical constituents: Contain NLT 2% of
alkaloids.
Alkaloids like Conessine (30%),
Norconessine, Isoconessine and kurchicine.
Uses: Antiprotozoal in dysentery and
malaria, astringent and tonic
T.S. of kurchi bark
Powder microscopy
• Cork cells: Thin walled, few are colourless
and few with yellowish brown matter.
• Stone cells: Rectangular to oval shape,
lignified, striated walls.
• Medullary rays: Parenchyma cell at right
angle
• Starch grains: Few, simple grains.
• Calcium oxalate crystals: Rhombic /diamond
shape.
• Phloem fibres are absent.
Micro-chemical tests
Reagent Observations Characteristics
Phloroglucinol +
conc. HCl
Pink colour Lignified stone cells
Iodine solution Blue colour Starch
Conc. Sulphuric acid Green colour Stone cells
Dil acetic acid Insoluble Calcium oxalate crystals
Dil. Hydrochloric
acid
Crystal soluble Calcium oxalate crystals
Sulphuric acid (60%) Needles of calcium
sulphate crystals
form on standing
Calcium oxalate crystals
Chemical tests
General chemical tests for alkaloids

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Microscopy of kurchi

  • 1. Microscopy of Kurchi bark Mr. Vipul. A. Sansare M.Pharm (Pharmaceutics) Contact No. :8692930764 Email :avipulsansare@gmail.com
  • 2. Synonym: Holarrhena, Kurchi BS: Dried stem bark of the Holarrhena antidysentrica Family: Apocynaceae. Collected from plants of 8 to 12 years old.
  • 3. Chemical constituents: Contain NLT 2% of alkaloids. Alkaloids like Conessine (30%), Norconessine, Isoconessine and kurchicine. Uses: Antiprotozoal in dysentery and malaria, astringent and tonic
  • 5. Powder microscopy • Cork cells: Thin walled, few are colourless and few with yellowish brown matter. • Stone cells: Rectangular to oval shape, lignified, striated walls. • Medullary rays: Parenchyma cell at right angle • Starch grains: Few, simple grains. • Calcium oxalate crystals: Rhombic /diamond shape. • Phloem fibres are absent.
  • 6. Micro-chemical tests Reagent Observations Characteristics Phloroglucinol + conc. HCl Pink colour Lignified stone cells Iodine solution Blue colour Starch Conc. Sulphuric acid Green colour Stone cells Dil acetic acid Insoluble Calcium oxalate crystals Dil. Hydrochloric acid Crystal soluble Calcium oxalate crystals Sulphuric acid (60%) Needles of calcium sulphate crystals form on standing Calcium oxalate crystals
  • 7. Chemical tests General chemical tests for alkaloids