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Alkaloids
• Natural/ synthetic
• Contain one or more nitrogen atoms
• Heterocyclic nature
• Basic nature
Chemical tests
Test Reagent Observation
Mayer’ s test Potassium mercuric iodide Cream coloured ppt
Drangendroff’s test Potassium bismuth iodide Reddish brown ppt
Wagner’s test Iodine potassium iodide Reddish brown ppt
Hager’s test Picric acid Yellow ppt
Alkaloids biosynthesis
Amino acids Alkaloid synthesized
Ornithine Tropane
Lysine Lupinine, Anabasin, Conneine
Phenylalanine Ephedrine, Mescaline, Prunasin
Tyrosine+Phenylalanine Opium, Dhurrin
Tryptophan Indole, Quinoline, Psilabin
Dopamine Emetine, Colchicine
Histidine Pilocarpine
Valine Linamarine
Isoleucine Lotaustraline
Tryptophan and tryptamine Reserpine
Indole alkaloid
• Ergot
• Nux vomica
• Physostigma
• Rauwolfia
• Vinca
Trick: PVR:NE
Name BS CC test
Physostigma Physostigma venosum
(Seeds): Leguminosae
Physostigmine ----
Vinca Catharanthus roseus(
whole plant):
Apocynaceae
Vincrstine,
vinblastine
----
Rauwolfia Rauwolfia serpentine:
(Root): Apocynaceae
Reserpine,
Ajmaline,Yohimbine
-----
Nux vomica Strychnos nuxvomica
(Seeds): Loganiaceae
Srychnine, brucine Mandinn’s test
Ergot Claviceps purpurea
(Fungi): Hypocreaceae,
clavicipitaceae.
Rye plant, Secale cereale
(Gramnae)
Peptide alkaloid,
Ergometrine,
ergotamine (D and
L form): L form is
active
Van-Urk’s test
Fluorescent test
Tropane alkaloid
• Belladonna
• Datura
• Hyoscymus
• Coca
Name BS CC test
Belladona Atropa belladonna (European)
Atropa acuminata (Indian).
solanaceae : leaves
Hyoscine,
Atrpine
Vitali-Morin
test
Datura
(Angel’s
trumpet)
Datura metel, solanaceae: leaves and
flowering tops
Hyoscine,
Hyoscine
esters
Vitali-Morin
test
Hyoscyamus
(Henbane)
Hyoscyamus niger. solanaceae: leaves
and flowering tops
Hyoscine Vitali-Morin
test
Coca Erythroxylum coca (Bolivia coca),
Erythroxylum truxillense (Peruvia
coca). Erythroxylaceae: Leaves
Cocaine,
cinnamyl
cocaine
-----
Quinoline alkaloids
Name BS CC test
Cinchona
(Peruvian
bark,
Jesuit’s
bark)
Cinchona
calisaya,
C. ledgeriana,
C.officinalis,
C. succirubra
Rubuiaceae:
Barks
Quinine, quinidine,
cinchonine,cinchonidine
Quinine content is high
in C. ledgeriana
Thallequin
test
Quinazoline alkaloids
Name BS CC test
Vasaka (Malabar
nut, Adulsa,
Adhatoda)
Adhatoda vasaka.
Acanthaceae:
leaves
Vasicine,
Vasicinone
-----
Proto/amino alkaloid
do not have heterocyclic ring with nitrogen, but are also derived
from amino acid
Name BS CC test
Ephedra
(Ma-Huang)
Ephedra gerardiana,
Ephedra nebrodensis.
Ephedraceae, Gnetaceae:
Young stems
Ephedrine -----
Steroidal alkaloid
Name BS CC test
Kurchi
(Holarrhena)
Holarrhena
antidysentrica.
Apocynaceae: bark
Kurchicine
(Conessine)
-----
Ashwagandha
(withania, Indian
ginseng, Winter
cherry)
Withania
somnifera.Solanace
ae: roots and stems
Withanine
(alkaloid)
-----

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Alkaloids

  • 2. • Natural/ synthetic • Contain one or more nitrogen atoms • Heterocyclic nature • Basic nature
  • 3. Chemical tests Test Reagent Observation Mayer’ s test Potassium mercuric iodide Cream coloured ppt Drangendroff’s test Potassium bismuth iodide Reddish brown ppt Wagner’s test Iodine potassium iodide Reddish brown ppt Hager’s test Picric acid Yellow ppt
  • 4. Alkaloids biosynthesis Amino acids Alkaloid synthesized Ornithine Tropane Lysine Lupinine, Anabasin, Conneine Phenylalanine Ephedrine, Mescaline, Prunasin Tyrosine+Phenylalanine Opium, Dhurrin Tryptophan Indole, Quinoline, Psilabin Dopamine Emetine, Colchicine Histidine Pilocarpine Valine Linamarine Isoleucine Lotaustraline Tryptophan and tryptamine Reserpine
  • 5. Indole alkaloid • Ergot • Nux vomica • Physostigma • Rauwolfia • Vinca Trick: PVR:NE
  • 6. Name BS CC test Physostigma Physostigma venosum (Seeds): Leguminosae Physostigmine ---- Vinca Catharanthus roseus( whole plant): Apocynaceae Vincrstine, vinblastine ---- Rauwolfia Rauwolfia serpentine: (Root): Apocynaceae Reserpine, Ajmaline,Yohimbine ----- Nux vomica Strychnos nuxvomica (Seeds): Loganiaceae Srychnine, brucine Mandinn’s test Ergot Claviceps purpurea (Fungi): Hypocreaceae, clavicipitaceae. Rye plant, Secale cereale (Gramnae) Peptide alkaloid, Ergometrine, ergotamine (D and L form): L form is active Van-Urk’s test Fluorescent test
  • 7. Tropane alkaloid • Belladonna • Datura • Hyoscymus • Coca
  • 8. Name BS CC test Belladona Atropa belladonna (European) Atropa acuminata (Indian). solanaceae : leaves Hyoscine, Atrpine Vitali-Morin test Datura (Angel’s trumpet) Datura metel, solanaceae: leaves and flowering tops Hyoscine, Hyoscine esters Vitali-Morin test Hyoscyamus (Henbane) Hyoscyamus niger. solanaceae: leaves and flowering tops Hyoscine Vitali-Morin test Coca Erythroxylum coca (Bolivia coca), Erythroxylum truxillense (Peruvia coca). Erythroxylaceae: Leaves Cocaine, cinnamyl cocaine -----
  • 9. Quinoline alkaloids Name BS CC test Cinchona (Peruvian bark, Jesuit’s bark) Cinchona calisaya, C. ledgeriana, C.officinalis, C. succirubra Rubuiaceae: Barks Quinine, quinidine, cinchonine,cinchonidine Quinine content is high in C. ledgeriana Thallequin test
  • 10. Quinazoline alkaloids Name BS CC test Vasaka (Malabar nut, Adulsa, Adhatoda) Adhatoda vasaka. Acanthaceae: leaves Vasicine, Vasicinone -----
  • 11. Proto/amino alkaloid do not have heterocyclic ring with nitrogen, but are also derived from amino acid Name BS CC test Ephedra (Ma-Huang) Ephedra gerardiana, Ephedra nebrodensis. Ephedraceae, Gnetaceae: Young stems Ephedrine -----
  • 12. Steroidal alkaloid Name BS CC test Kurchi (Holarrhena) Holarrhena antidysentrica. Apocynaceae: bark Kurchicine (Conessine) ----- Ashwagandha (withania, Indian ginseng, Winter cherry) Withania somnifera.Solanace ae: roots and stems Withanine (alkaloid) -----