This document summarizes the MediaDNA project, which investigates emerging fingerprinting technologies to help uncover relationships between disparate audiovisual materials. The project brings together a network of organizations to apply these technologies for public use and digital humanities research. The goals are to help search collections, understand how content circulates online, and stimulate public engagement with cultural heritage. Challenges include scaling the technologies beyond proof of concepts and addressing issues of privacy and control of online content.
Doing Television History Outside the Box: Unexplored Territories on the Europ...EUscreen
Presentation by Dana Mustata about doing television history outside the box at the Second EUscreen International Conference on Use and Creativity, which took place at the National Library of Sweden, Stockholm, on September 15-16, 2011.
Transcribathons as citizen science projects: a comparative analysis of Europ...Web2Learn
Presentation at the Natural History Museum Berlin, October 28-29, 2021. Symposium "Participatory Transcription Projects in Museums, Archives, Libraries - A Practical Exchange of Experience" https://www.museumfuernaturkunde.berlin/en/museum/events/participatory-transcription-projects-museums-archives-libraries-a-practical-exchange
Doing Television History Outside the Box: Unexplored Territories on the Europ...EUscreen
Presentation by Dana Mustata about doing television history outside the box at the Second EUscreen International Conference on Use and Creativity, which took place at the National Library of Sweden, Stockholm, on September 15-16, 2011.
Transcribathons as citizen science projects: a comparative analysis of Europ...Web2Learn
Presentation at the Natural History Museum Berlin, October 28-29, 2021. Symposium "Participatory Transcription Projects in Museums, Archives, Libraries - A Practical Exchange of Experience" https://www.museumfuernaturkunde.berlin/en/museum/events/participatory-transcription-projects-museums-archives-libraries-a-practical-exchange
Research and Development at Sound and Vision Victor de Boer
Slides for guest lecture about R&D at the Netherlands Institute for Sound and Vision for the lecture series "Introduction to IMM" at VU Amsterdam.
With slides by Lotte Belice Baltussen, Maarten Brinkerink, Johan Oomen, Bouke Huurnink and Victor de Boer
Data modelling at Europeana and DM2E - SMW13Antoine Isaac
Presentation on how the Eduorpeana Data Model is used and extended in the Europeana and DM2E projects.
Made for the Semantic Media Web innovation day, Berlin, Sept 27, 2013: http://semantic-media-web.de/innovationsforum/metadaten/
3D Digitizing a whole museum: a metadata centered workflow3D ICONS Project
Guidi, G., Rodríguez Navarro, P., Micoli, L.L., Gonizzi Barsanti, S., Russo, M., "3D Digitizing a whole museum: a metadata centered workflow", 2013 Digital Heritage International Congress (DigitalHeritage), Vol. 2, pp. 307-310, IEEE, 2013. ISBN 978-1-4799-3169-9.
ViBRANT—Virtual Biodiversity Research and Access Network for TaxonomyVince Smith
Presented by Dave Roberts and coauthored by Vince Smith at BioIdentify 2010, the National Muséum of Natural History (MNHN), Paris, France. 20-22 Sept, 2010.
Europeana Publishing Framework (Concept) at Culture JamEuropeana
Presentation given by Paul Keller (kennisland) and Harry Verwayen (Europeana) at the culture jam conference, Vienna July 9 2015. It explains the concept of the new publishing framework that supports cultural institutions participating in Europeana to share their material more openly and in higher quality.
Presentation at the H2020-CEF Infoday, 16 January 2014 http://ec.europa.eu/digital-agenda/en/news/information-and-networking-days-h2020-work-programme-2014-2015-connecting-europe-facility
Europeana and the relevance of the DM2E resultsAntoine Isaac
Presentation on the value of results of the DM2E project, from the Europeana perspective.
Presented at the DM2E final event, Pisa, Dec 11 2014
http://dm2e.eu/dm2e-final-event-registration-and-agenda/
Maarten Brinkerink and Johan Oomen (Netherlands Institute for Sound and Vision, NL) will talk about Waisda?, an open source video labeling game framework developed by Sound and Vision[3], which is currently being developed further in the context of Europeana.[4] Sound and Vision has collaborated with several public broadcasters in the Netherlands to enable fans of certain programmes to contribute fine-grained description of this content. In the latest edition called ‘Spotvogel’ (Mockingbird) Sound and Vision collaborated with the nature TV programme ‘Vroege Vogels’ (Early Birds, by the VARA) to mobilize the online community around the programme for identifying flora, fauna and locations within specific segments of the broadcasts. To support the tagging of the flora and fauna the game utilized a controlled vocabulary that is maintained by Naturalis. Players are awarded points when their tag entries match with other players, and they can score bonus points for using ‘professional’ terms from the controlled vocabulary. Players can also earn badges for certain achievements within the game, for instance for identifying a certain number of birds. Up until now the game managed to gather over 240,000 tags.
Research and Development at Sound and Vision Victor de Boer
Slides for guest lecture about R&D at the Netherlands Institute for Sound and Vision for the lecture series "Introduction to IMM" at VU Amsterdam.
With slides by Lotte Belice Baltussen, Maarten Brinkerink, Johan Oomen, Bouke Huurnink and Victor de Boer
Data modelling at Europeana and DM2E - SMW13Antoine Isaac
Presentation on how the Eduorpeana Data Model is used and extended in the Europeana and DM2E projects.
Made for the Semantic Media Web innovation day, Berlin, Sept 27, 2013: http://semantic-media-web.de/innovationsforum/metadaten/
3D Digitizing a whole museum: a metadata centered workflow3D ICONS Project
Guidi, G., Rodríguez Navarro, P., Micoli, L.L., Gonizzi Barsanti, S., Russo, M., "3D Digitizing a whole museum: a metadata centered workflow", 2013 Digital Heritage International Congress (DigitalHeritage), Vol. 2, pp. 307-310, IEEE, 2013. ISBN 978-1-4799-3169-9.
ViBRANT—Virtual Biodiversity Research and Access Network for TaxonomyVince Smith
Presented by Dave Roberts and coauthored by Vince Smith at BioIdentify 2010, the National Muséum of Natural History (MNHN), Paris, France. 20-22 Sept, 2010.
Europeana Publishing Framework (Concept) at Culture JamEuropeana
Presentation given by Paul Keller (kennisland) and Harry Verwayen (Europeana) at the culture jam conference, Vienna July 9 2015. It explains the concept of the new publishing framework that supports cultural institutions participating in Europeana to share their material more openly and in higher quality.
Presentation at the H2020-CEF Infoday, 16 January 2014 http://ec.europa.eu/digital-agenda/en/news/information-and-networking-days-h2020-work-programme-2014-2015-connecting-europe-facility
Europeana and the relevance of the DM2E resultsAntoine Isaac
Presentation on the value of results of the DM2E project, from the Europeana perspective.
Presented at the DM2E final event, Pisa, Dec 11 2014
http://dm2e.eu/dm2e-final-event-registration-and-agenda/
Maarten Brinkerink and Johan Oomen (Netherlands Institute for Sound and Vision, NL) will talk about Waisda?, an open source video labeling game framework developed by Sound and Vision[3], which is currently being developed further in the context of Europeana.[4] Sound and Vision has collaborated with several public broadcasters in the Netherlands to enable fans of certain programmes to contribute fine-grained description of this content. In the latest edition called ‘Spotvogel’ (Mockingbird) Sound and Vision collaborated with the nature TV programme ‘Vroege Vogels’ (Early Birds, by the VARA) to mobilize the online community around the programme for identifying flora, fauna and locations within specific segments of the broadcasts. To support the tagging of the flora and fauna the game utilized a controlled vocabulary that is maintained by Naturalis. Players are awarded points when their tag entries match with other players, and they can score bonus points for using ‘professional’ terms from the controlled vocabulary. Players can also earn badges for certain achievements within the game, for instance for identifying a certain number of birds. Up until now the game managed to gather over 240,000 tags.
The EUscreen project aims to promote the use of television content to explore Europe's rich and diverse cultural history.
It will create access to over 30,000 items of programme content and information, and by developing a number of interactive functionalities and dynamic links with Europeana it will prove valuable to the widest range of cultural, educational and recreational users.
EUscreen started in October 2009 and the project consortium, which includes 27 partner institutions from around Europe, is being co-ordinated by Utrecht University.
More information about the project can be found on www.euscreen.eu
The European (Digital) Library - Overview and OutlookOlaf Janssen
The European Library (www.theeuropeanlibrary.org) is a multilingual portal offering integrated access to the tens of millions of resources (books, magazines, journals...) of 18 national libraries in Europe. It offers free searching and delivers both digital and non-digital objects. It provides a vast virtual collection of mate-rials from all disciplines. The European Library is currently being expanded with the holdings of the national libraries of the 10 EU New Member States. From September 2006 onwards the remaining EU and EFTA na-tional libraries will be connected to TheEuropeanLibrary.org, bringing the total number of participating na-tional libraries to ±35 by the end of 2008.
In the beginning of 2006 the EC expressed support for The European Library to evolve into a much bigger European Digital Library (EDL), including access to the digital collections of other major cultural heritage institutions, such as museums and archives. The EDL is planned to include the holdings of all European na-tional libraries and a minimum of 2M digital works by the end of 2008. By 2010 the EDL needs to have ex-panded to include collections of archives, museums and other libraries, with a minimum of 6M digital works.
The European Library aims to remain a major player in the European cultural heritage field and is already strengthening its cooperation with other relevant key initiatives, such as MACS, DELOS, MICHAEL, BRICKS and MINERVA.
Janssen, O.D. (2006), “The European (Digital) Library - Overview and Outlook”, in: The e-volution of Information Communication Technology in Cultural Heritage, Joint event CIPA/VAST/EG/EuroMed, Project papers, M. Ioannides, D. Arnold, F. Niccolucci, K. Mania (Eds.), EPOCH publication, 2006, pp. 189-193 (and on CD-ROM)
Challenging Television Historiography in an Analogue WorldEUscreen
Presentation by Dana Mustata (RUG) at the NECS Conference 2012 in Lisbon, Portugal, in the panel 'Unstable Histories: The Problem of Seeing and Understanding 'Old' Television in the Digital Age'.
European perspectives on design for learning in the 21 centuryTeemu Leinonen
Keynote at the National Conference about flexible learning, 15-17 July Wrest Point Conference Centre, Hobart, Tasmania, Australia / Australasian Association of Distance Education Schools.
In this address, Professor Leinonen will discuss ‘meta-design’, which means design of ‘things’ for educators to design their own teaching and for learners to design their own learning. He will also present a generic Finnish / Northern European perspective on ICT in education, which he and his colleagues aim to make a pan-European model through a project entitled Innovative Technologies for an Engaging Classroom (iTEC). iTEC is a four-year, large-scale project that takes an informed look the potential classrooms of the future. With 27 project partners, including 14 Ministries of Education and funding from the European Commission of 9.45 million Euros, iTEC will provide a model describing how the deployment of technology in support of innovative teaching and learning activities can move beyond small scale pilots and become embedded in all Europe's schools. iTEC is being piloted in over 1,000 classrooms in 12 countries, making it by the most significant pan-European validation of ICT in schools yet undertaken.
Through a new Audiovisual Think Tank, visionary experts in the AV cultural heritage sector are working together to map out our shared strategic priorities and put into place a research and action agenda to shape the coming decade. The AV Think Tank looks to represent major AV archives and digital cultural heritage professionals from across the globe and closely connects these key players to work collectively at the forefront of the sector in consultation with the wider community. Initiated and actively supported by Sound and Vision, the AV Think Tank aims to lay the groundwork for an AV archiving sector that enables more long-term use of, learning with, and education through AV materials.
Models and Tools for Knowledge ReconstructionPaolo Nesi
International Conference “Tools and Applications for the Dissemination of Cultural and Linguistical Heritage”aBologna, 23 April 2014
Knowledge Work-flow: from Sources to Final Users
Data Integrity: Accuracy and Reliability of Data
Web Crawling and Data Mining
Link Discovering
NLP - Natural Language Processing Phases
Web Ontologies and Languages
ECLAP: E-Library for Performing Arts
The OSIM Project - Open Space Innovative Mind
Digital Cultural Heritage and the new EU Framework Programmelocloud
2nd LoCloud CY Awareness Event at the Ministry of Education and Culture.
Presentation delivered by Marinos Ioannides, Cyprus University of Technology
Cyprus
5 March 2014
Open, Smart and Connected access to Audiovisual CollectionsJohan Oomen
Talk given at COPEAM 2018.
“Heritage and Media – Preserving the future through our past: an opportunity for growth and democracy?”
Calviá - Mallorca, 10-12 May 2018
Hotel Meliá Calviá Beach
Calle Violeta, 1 Calviá Beach - 07181 Mallorca, Spain
Cultural heritage embraces resources inherited from the past and offers a great variety of opportunities to the present: monuments, sites and traditions, but also visual arts, cinema, TV and radio archives.
In this framework, the Media of the Euro-Mediterranean region – both traditional and new ones – have to play their role, particularly given the challenges that such issue implies in terms of content production, audiovisual documents preservation and impact of the digital transition as a tool for the safeguard and enhancement of our common heritage.
New approaches towards accessing digital audiovisual heritage What will EUscr...Johan Oomen
During the past decade, a massive body of European audiovisual heritage has become accessible online: on video sharing sites and websites of archives, or through initiatives such as EUscreen.eu and Europeana.eu. Once online, audiovisual heritage circulates in diverse ways: users watch, share, like, or dislike it; they comment, appropriate, and download videos for remix and recirculation. It thus becomes part of the popular consumption of history, potentially creating new interpretations of heritage materials, challenging authorised perspectives.
Challenges to audiovisual heritage online
Heritage institutions perceive the consequences of the recent technological transformations of the sector as a major challenge and opportunity. Nevertheless, urgent questions regarding the circulation of audiovisual heritage online remain unanswered:
How do strategies of curation shape the appropriation of digitized heritage?
How does digitisation and online circulation of audiovisual heritage affect the mission, role, and structure heritage institutions, as well as their relationships with media producers and publics?
How can audiovisual archives better foster the re-use of Europe’s audiovisual heritage?
(How) do digital curation and other appropriations of audiovisual heritage create new perspectives on European history and identity do ?
How does online circulation of audiovisual heritage alter the power relationships between amateur and professional historians in a public history environment, potentially blurring the boundaries between authorised and popular visions of European history?
What new tools and methods do we need to analyse the circulation of audiovisual heritage online, and how have traditional methods to be adapted for this aim?
To discuss these challenges, Utrecht University's Centre of Television in Transition in collaboration with The Netherlands Institute for Sound and Vision and CLARIAH will organize a one day symposium on May 16th, 2018.
DIVE+: Explorative Search for Digital HumanitiesJohan Oomen
DIVE+ is an event-centric linked data digital collection browser aimed to provide an integrated and interactive access to multimedia objects from various heterogeneous online collections. It enriches the structured metadata of online collections with linked open data vocabularies with focus on events, people, locations and concepts that are depicted or associated with particular collection objects. DIVE+ is result of a true inter-disciplinary collaboration between computer scientists, humanities scholars, cultural heritage professionals and interaction designers. The tool allows humanities scholars to explore unexpected relations between entities and media objects and to construct and share navigation paths to develop research narratives.
Preserving Interactive Media - SXSW 2017Johan Oomen
http://schedule.sxsw.com/2017/events/PP96792
Interactive documentaries are at the vanguard of current media technologies. Taking into account every framework imaginable, its makers challenge some of our assumptions about how these technologies can or cannot support bringing a non-fiction storyline to a audience. In over a decade of IDFA DocLab’s existence, web technologies have changed dramatically and many producers experience how complicated it can be to keep their creations accessible and ‘experienceable’.
In this panel, chair Johan Oomen from Sound and Vision, will outline the challenges to creating dynamic web archives. We will then take a deeper look at particular cases. NFB collaborated with Google on the re-making of Bear 71 - porting it from a Flash-based to a WebVR online experience. Megan Lindsay will present this collaboration on re-representing a modern classic. After the presentations, there will be room for questions.
Slides presented by Jan Müller (CEO Netherlands Institute for Sound and Vision) at the CLARIAH kick-off.
http://www.clariah.nl/activiteiten/clariah-kick-off
The general aim of LinkedCulture is to describe how the information need of the Tussen Kunst & Kitsch (Antiques Roadshow) viewers can be satisfied from both their couch and on-the-go, supporting both passive and more active needs. Linking to external information and content, such as Europeana, museum collections but also auction information has been incorporated in these scenarios
Towards more smart, connected and open audiovisual archivesJohan Oomen
As a result of digitisation of analogue holdings and working processes, more and more material from audiovisual archies is being made available online. This marks a transformative shift, as archives and users are now sharing the same information space. Once digital and part of an open network, objects from audiovisual archives can be shared, recommended, remixed, embedded, cited, referenced to and so on. It is a far cry from several years ago, when users were obliged to visit brick and mortar institutions to access collections. This shift towards digital enables archives to fulfil their pubic missions better; crossing geographical boundaries, using new channels for content distribution, engage with user groups and use new technologies to make work processes more efficient and allow for new access points to collections. It also introduces fundamental challenges, forcing audiovisual archives to [1] rethink their role and function in the value chain of media production and modern society at large, [2] assess which activities and competences are vital to succeed in a digital context.
We envision the future audiovisual archives to be smart, connected and open; using smart technologies to optimise workflows for annotation and content distribution. Collaborating with third parties to co-design and co-develop new technologies in order to manifest themselves as frontrunners rather than followers. Being connected to other sources of information (other collections, contextual sources), to a variety of often niche user communities, researchers and the creative industries. To embrace the use of standards defined by external instances rather than by the cultural heritage communities themselves. Fully embrace ‘open’ as the default to have maximum impact in society: applying open licences for content delivery, using open source software and open standards wherever possible. Promote open access to publications and so on.
This keynote examines how the public mission of archives (i.e. supporting a myriad of users to utilize collections to learn, experience and create) can be achieved in a digital context. It addresses the challenges related to the role and function of institutions and provides practical insights in how archives can establish a culture of innovation to manage challenges they face today. It addresses some of the major questions audiovisual archives are faced with today.
Hoe breng je aan elkaar gerelateerde openbare gegevens ook echt met elkaar in verbinding? Die vraag brengt een grote groep mensen vanuit het bedrijfsleven, kennisinstituten en de overheid bijeen in de pilot Linked open data. Nadat in 2012/2013 de mogelijkheden voor het prepareren van data, het daadwerkelijke linken van data en het toepassen van linked data zijn onderzocht, is in november 2013 een vervolg van start gegaan: het Platform implementatie Linked Open Data (PiLOD). Focus van het PiLOD ligt op het toepassen en werken met Linked Open Data.
Op weg naar een Nederlandse Erfgoedthesaurus met Linked Open DataJohan Oomen
Steeds meer collectiebeheerders zijn bezig om de mogelijkheden voor eindgebruikers van Linked Open Data te onderzoeken en in praktijk te brengen. Door het toevoegen van externe informatie aan de eigen collectie (contextualisering) en het verbinden van de eigen collectie aan externe informatiebronnen wordt de collectie onderdeel van een groter geheel en ontstaat er een dynamische relatie van de inhoud van de eigen collectie met de buitenwereld. De thesauri van erfgoedinstellingen zijn bij uitstek geschikt om externe bronnen te verbinden. RCE, Nederlands Instituut voor Beeld en Geluid en Naturalis sloten een convenant om een erfgoedthesaurus voor het Nederlandse erfgoeddomein te ontwikkelen. Hiermee leggen zij de basis van een netwerk van thesauri, zodat instellingen en externe kennisnetwerken rijke verbindingen kunnen realiseren. Als onderdeel van dit proces ontwikkelen zij een gemeenschappelijke set tools die vrijelijk aan de Nederlandse erfgoedsector ter beschikking zullen worden gesteld. De presentatie toont de meerwaarde van Linked Open Data voor de erfgoedsector en plaatst de Nederlandse Erfgoedthesaurus in de context van de infrastructuur voor het erfgoeddomein. Verder zal de “Linked Open Data demonstrator” worden gepresenteerd, zoals deze door Beeld en Geluid en de RCE is gerealiseerd.
The many unexptected joys if being "out there": examples of user participatio...Johan Oomen
Contribution as part of the SXSW 2014 panel "100 Years of Oversharing: Tools for Time Travel" - http://schedule.sxsw.com/2014/events/event_IAP21645 @johanoomen
A typed journal from WWI passed on through generations fuels a young man's dreams of time travel and allows us to explore the power of personal stories and photos. Together with archival collections, these items take us through space and time, and the magical ability of cultural memory institutions to help individuals bring these incredibly compelling dreams to life. The World Wide Web provides the cultural, technological, and legal frameworks to open the doors to innovation and imagination, and also enables libraries, archives and museums the world over to play a critical role. We explore some of the diverse efforts to bring stories and memory to life in new ways, while also fostering open data and preservation, and the pros and cons at the intersection of public domain and private enterprise.
Europeana Awareness year 2 review slides for Workpackage 2 'End-user engagement'Johan Oomen
General information on the project: http://pro.europeana.eu/web/europeana-awareness
Scope of this presentation: following the success of the first year, WP2 continued to deliver results in this reporting period. Overall progress for this year has been good, with no major deviations from the work plan. The content gathering campaigns for Europeana 1914-1918 were successful, both in terms of public engagement and content added as well as audience reach.
This year also saw the launch of the Europeana 1989 campaign, aiming to create a digital archive of memorabilia connected to the fall of the Iron Curtain. Besides the objects that were gathered, the campaign provided important insights into the challenges of managing a campaign which deals with more recent events. These lessons have been documented and will be taken on board in future campaigns.
The collaboration with the Wikipedia community was launched in January and continued throughout the year. A great variety of events were organized as part of the project, including an ambitious international photo competition “Wiki Loves Public Monuments.” As one of the results, a strategic plan for future collaboration between the two communities (Europeana Network and the Wikipedia community) was written in December.
Europeana Sounds kick-off - Workpackage 2 Enrichment and ParticipationJohan Oomen
The objective of this workpackage is to support discovery and use by improving metadata through innovative methods including semantic enrichment and crowdsourcing. It coordinates the design and implementation on mechanisms to improve the quality of existing metadata and contextual information. This will support enhanced exploration, deepen understanding of the collections, and will increase end-user engagement. Significantly increase quality of existing and new Europeana metadata for audio and audio-related items though: (a) active participation with existing audiences; (b) machine-driven tools.
Specific goals
• Offer tools for metadata tagging and contextualisation to the wider community. This will (1) increase quality and user satisfaction in terms of content discovery; (2) promote increased engagement between institutions and their audiences.
• Apply semantic web technologies to enable enrichment of the Europeana Sounds collections. This will increase quality of the metadata and user satisfaction in terms of content discovery.
• Collaborate with Wikimedia chapters in Europe to add contextual knowledge on the Europeana Sounds collection. Six edit-a-thons (campaigns that aim to create wiki pages on focussed areas) will be organised in year two and three of the project. This will (1) add a layer of in-depth knowledge to the collections presented online;
(2) strengthen links between Europeana, the Europeana Network and the international Wikipedia community.
• Align music scores to text, to forge a dynamic connection between currently separated collections. By allowing for new types of exploration, the value for end-users of both the multimedia and digitised paper-based resources will be increased.
• Explore possibilities of music information retrieval to support innovative, language independent exploration of audio collections.
• Put in place policies and (in connection with WP5) infrastructural preconditions allowing enrichments to be re-ingested in the information systems of the contributing archives, wherever relevant.
BRIDGE addresses access to television archives. Television is not an isolated medium, but a key node in a complex information landscape. From an exclusive focus on producing radio, television and publishing program guides, broadcasting organisations have moved to cross-media production companies where users are directly involved in online follow-up discussions. To be meaningful, these additional materials need to be cross-linked to the traditionally archived television materials so as to arrive at a meaningful web of information.
The project will develop, test and deploy efficient, effective and robust methods for automatically creating meaningful links between dynamically expanding archived television data and other information sources that are centered around entities, themes and events.
More: http://ilps.science.uva.nl/node/735
Op 9 november is er een Hackathon op het Mediapark in Hilversum. Het thema is Omroep Data. Er komen allerlei interessante datasets beschikbaar, verder kan je kan teamen met programmamakers, omroepers, webguru’s, maar ook je eigen App uitbouwen. Deze Hackathon Publieke Omroep wordt mede mogelijk gemaakt door NPO, STER en Beeld en Geluid. - See more at: http://www.hackdeoverheid.nl/hackathon-publieke-omroep-2/#sthash.Zxjjw8OR.dpuf
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
1. MediaDNA
Investigating emerging technologies in Humanities
research to serve the public use of digitized
audiovisual material
DARIAH-EU Innovation Forum #1
Aarhus, November 3, 2017
Prof. Eggo Müller, Professor of Media and Communication. Utrecht University
Johan Oomen, Head of Research, Netherlands Institute for Sound and Vision
8. MediaDNA - network
Netherlands Institute for Sound and Vision (NL)
Utrecht University (NL)
Digital Humanities Lab, Umeå University (SE)
Centre for Contemporary and Digital History (LUX)
Institute for Contemporary European History (CZ)
EUscreen Foundation EUscreen.eu
EUROCLIOH (European Association of History Educators)
Europeana Foundation Europeana.eu
10. MediaDNA - objectives
DARIAH-EU grant theme “Public Humanities” to…
Initiate a Trans-European network of digital humanities researchers,
audiovisual archives, educators and software developers.
Investigate and test multimedia fingerprinting and –tracking
technologies for use in the in the public domain.
Create understanding of how these emerging technologies can help
search collections and uncover previously hidden relations between
disparate items.
Stimulate truly public engagement with audiovisual heritage.
11. MediaDNA - actions
Research proposal “European History Reloaded: Circulation
and Appropriation of European audiovisual Heritage”
(CADEAH) to Horizon 2020 JPICH call “Digital Heritage” (June
2017)
Symposium “Video Tracing and Tracking in Digital Humanities
Research” (Hilversum, 13 October 2017)
Symposium “The Many Lives of Europe’s Audiovisual Heritage
Online” (Utrecht, 16 May 2018)
Open access publication on the topic (2018)
12. MediaDNA - challenges
steered by tools by questions
particular collections the Internet
curation machines
proof of concepts scalability
13.
14. Eggo Müller, Utrecht University
Johan Oomen, Netherlands Institute for Sound and Vision
15. MediaDNA - motivation
Audiovisual material is central to our daily lives and the remembrance of
the, eventful, 20th century.
More and more films, television and radio programmes, photographs and
related printed materials become digitized and thus are accessible online
for consumption and re-use.
Re-use in the public sector (education, broadcasters) supports the
integration of the imaginative audiovisual to our material and collective
cultural memory, and in line with this a greater understanding of notions of
identity; of who we are and where we belong.
At the same time though, the availability of digitized audiovisual material
fosters piracy and the 'unofficial' re-use of the resources circulating online.
20. Fingerprinting technologies
Four main application domains:
metadata extraction and audio forensics (item-based)
dataset analysis (segment matching, audio phylogeny)
integrating different tools and multimodal analysis (see MICO,
OSS framework)
privacy-aware personalization and recommendation based on
a combination of content-based recommendation and
collaborative filtering
25. European History Reloaded:
Circulation and Appropriation of
Digital European Audiovisual Heritage
Proposal to EU Horizon 2020 JPI Cultural Heritage -
Digital Heritage
circulation an reuse of archival partner’s contributions beyond
the EUscreen platform
tracing and tracking of reused items/fragments
understanding popular appropriation of European heritage
advising archives about their online policies and curation
fostering user engagement and reuse
26. MediaDNA: new research instruments
“Watermarking and fingerprinting are exiting technologies that can
facilitate critical business functions, including content identification,
copyright control, behavior tracking, copy protection and forensics. In
addition, these technologies provide the means for content owners who
embrace emerging distributions platforms, such as user generated
content websites, to develop new revenue models based on what they
previously might have considered pirated content.” (Milano 2012)
27. Symposium
“Video Tracing and Tracking in Digital
Humanities Research”
Netherlands Institute for Sound and Vision, 13 October 2017
Explore the state of the art of fingerprinting technologies
28. Symposium
“The Many Lives of Europe’s Audiovisual
Heritage Online”
Utrecht University, 16 May 2018
Explore the circulation and appropriation of audiovisual
heritage online