Lyme disease is caused by the bacterium Borrelia burgdorferi and its symptoms include a characteristic rash called erythema migrans. If left untreated, later symptoms can include joint pain, heart palpitations, and shooting pains. Diagnosis is made through ELISA testing which detects antibodies produced by the immune system against Borrelia antigens like OspC, Crasp, and VIsE. The Borrelia IgM ELISA uses recombinant antigens for high sensitivity and specificity in detecting IgM antibodies in serum or plasma samples, with results available in under two hours.

1. Lyme
Disease

2. Agenda

3. Introduction

4. Lyme Disease

5. Lyme Disease
BASIC INFORMATION ABOUT THE DISEASE

6. Pathogen- Borrelia burgdorferi
CHARACTERISTIC APPERANCE

7. Symptoms
IF UNTREATED

8. ELISA

9. Testing Diagnosis

10. Borrelia ELISA

11. Antigens vs Antibodies
IgM,IgG
VIsE
BmpA
Crasp
OspC

12. Borrelia IgM ELISA

13. Borrelia IgM ELISA Assay Principle

14. Procedure

15. Principle of Assay

16. Calculation of Results

17. Qualitative Calculation for
Serum/Plasma

18. Advantages
Antigens
Recombinant antigens lead to
better standardization and lot-
to-lot consistency
Sensitivity
High sensitivity and specificity
confirmed by clinical samples
Controls
Controls included: positive /
cut-off / negative
Easy
Easy test procedure for
manual and instrument use
Fast
Total incubation time less than
2 hours

19. Significance of VIsE (antigen)
SWITCHING AT VISE IN BORRELIA
BURGDORFERI VISE PROTEIN

20. Questions

21. Interpretation

22. References
Borrelia IgM ELISA Kit for CSF & serum | BI-
21042 | Biomedica (bmgrp.com)