GenapSys™ has developed a novel electrical-based platform capable of accurately detecting single base incorporations. On the surface of the CMOS sequencing chip (Complementary Metal Oxide Semiconductor), there are millions of sensors, each designed to capture one clonally amplified bead. DNA sequencing technology.

1. GenapSys Sequencing
Group 9 | Melvin Alex | VIT Bhopal

2. Why Genapsys sequencing
method ?
Accurate, Robust, Affordable, Scalability

3. Project Overview
• Introduction
• Work Flow
• Technology
• Results
• Applications
• Conclusion and Questions

4. Introduction
• Novel sequencing by synthetic
approach that employs electrical
detection of nucleotide.
• Capable of generating highly accurate
DNA sequence information.
• Sequencing is carried out on
microfluidic chips that have a scalable
number of detectors.

5. Overview of Genapsys sequencing platform

6. Procedure
Step 1:
• Library
preparation
Step 2:
• Clonal
Amplification
Step 3:
• Sequencing
Step 4:
• Data
Analysis

7. 4-Step Workflow
Library
preparation
• Sequencing
libraries were
generated by
random shearing,
end repair, A-
tailing, ligation of
adapters and PCR
Clonal
Amplification
• Clonally amplify
libraries on the
GenapSys
Sequencing Prep
System.
Sequencing
• Load amplified
beads into the
chip and
sequence using
the GenapSys
Sequencer.
Data Analysis
• Basecall and
generate FASTQ
with Genapsys
analysis software.
Perform alignment
and variant
calling.(sequence
assembly)

8. Library preparation Clonal amplification Sequencing

9. CMOS
sequencing chip
• Chip contains millions of
individual sensors, which are
each loaded with a clonally
amplified bead.
• The electrodes in each sensor
measure minute changes in
impedance when nucleotides are
incorporated opposite the bead-
bound templates.
• The magnitude of the differential
signal correlates with the number
of incorporated nucleotides.
• Reusable chips.

10. RESULTS AND
OBSERVATIONS

11. Data/Observations
1
2.5
3
5.5
8.5 8.5
0
2
4
6
8
10
12
0 2 4 6 8 10 12 14
Genapsys

12. Applications
• Targeted resequencing
• Small genome sequencing
• Gene editing validation
• 16S rRNA-sequencing
• Targeted mRNA sequencing
• Small RNA sequencing
• Whole genome sequencing and others

13. Conclusion and Questions
• Small, low cost instrument : somewhere between Illumina iSeq100 and ONT
MiniON/Flongle
• Short reads ,relatively high quality similar to Illumina, but single end.
• Aiming initially at targeted panels
• How difficult is the sample prep? Can it become a point of care device?
• Will throughput go up? What will be the speed of 30x human genome?

15. Works Cited
• https://doi.org/10.1101/604553 (High accuracy DNA sequencing on a small,
scalable platform via electrical detection of single base incorporations)
• www.genapsys.com