LVG(Lymphogranuloma venereum) antigen test is laboratory diagnostic test done for identifying LVG antigen for the diagnosis of LGV.

2. Detection of LGV ANTIGEN IN PUS

3. • LVG stands for “Lymphogranuloma
Venereum”.
• LVG antigen is an antigen which enters in a
human body, causes LVG and shows symptoms
after 2-21 days.

4. DETECTION OF LVG ANTIGEN IN PUS
• Pus is obtained from bubo ( an enlarged
lymph node that is tender and painful ) With
monoclonal antibodies using
immunofluorescence method.
• Pus can be collected from rectum, genital
tract.

5. Monoclonal antibodies
• MA are laboratory produced antibodies from
one cell.
Immunofluorescence method
Immunofluorescence (IF) or cell imaging
techniques rely on the use of antibodies to label a
specific target antigen with a fluorescent dye
(also called fluorophores or fluorochromes)

6. IMMUNOFLUORENCE METHOD
Immunofluorescence (IF) or cell imaging
techniques rely on the use of antibodies to
label a specific target antigen with a
fluorescent dye (also called fluorophores or
fluorochromes)

7. • Combination of Monoclonal antibodies
and immunofluorescence method helps for
the detection and visualization of proteins, or
other antigens, in cells using antibodies.

8. Procedure
Articles required
Swab
Normal saline or PBS Solution
Syringe (18 gauze needle)
Clary Blair transport medium tube
Durable marker for writing on sample
Gauze pad
Adhesive tap
Skin anti-septic solution

11. PROCEDURE
• Hand washing using proper technique.
• Before entering patient room, assemble all
equipment.
• REMOVE ALL PERSONAL ITEMS (jewellery,
watches, mobile phones, pens, etc.
• Put on all personal protective equipment
(PPE)
• Again,

12. CONTINUE..
• Perform hand hygiene. Duration of the entire
procedure: 40-60 sec if hand washing with
soap and water; 20-30 sec if hand rubbing
with an alcohol-based solution.(WHO
recommended)
• Prepare room.
• Identify and prepare the patient.
• Locate the bubo and disinfect the skin over
and around the bubo with the alcohol pad

13. CONTINUE
• Aspirate the PBS solution (from collection kit)
or saline solution into syringe ( This will help
make aspiration of the bubo pus easier).
• Immobilize the bubo with your gloved hand.
• Put the needle into the bubo at a
perpendicular angle.

14. • Inject a few millilitres of saline solution or PBS
solution into bubo.
• Aspirate the pus . (Collect a minimum of 2
millilitres of bubo pus).
• Withdraw the needle gently.
• Transfer pus from the syringe to the swab
using the syringe and needle. DO NOT remove
the needle.

15. • Once bubo pus is on the swab, push the swab
into the Cary-Blair transport medium tube.
• Labelling should be done including type, date,
• Identity of the patient.
• The sample is now ready for shipment to the
National Reference Laboratory.
• Sample is send for laboratory analysis.
• Remove PPE.
• Perform hand hygiene.

16. • Report (24-48 hour)