This document discusses the four levels of measurement: nominal, ordinal, interval, and ratio. Each level provides more information than the previous. Nominal measurement simply categorizes objects into groups with no ordering. Ordinal adds rank ordering but distances between ranks are meaningless. Interval maintains equal distances between ranks and allows for subtraction and addition, but not ratios. Ratio is the highest level with all interval properties plus a true zero point, meaning ratios are meaningful. Appropriate statistics depend on the level of measurement.
Topic: Frequency Distribution
Student Name: Abdul Hafeez
Class: B.Ed. (Hons) Elementary
Project Name: “Young Teachers' Professional Development (TPD)"
"Project Founder: Prof. Dr. Amjad Ali Arain
Faculty of Education, University of Sindh, Pakistan
This presentation includes an introduction to statistics, introduction to sampling methods, collection of data, classification and tabulation, frequency distribution, graphs and measures of central tendency.
Topic: Frequency Distribution
Student Name: Abdul Hafeez
Class: B.Ed. (Hons) Elementary
Project Name: “Young Teachers' Professional Development (TPD)"
"Project Founder: Prof. Dr. Amjad Ali Arain
Faculty of Education, University of Sindh, Pakistan
This presentation includes an introduction to statistics, introduction to sampling methods, collection of data, classification and tabulation, frequency distribution, graphs and measures of central tendency.
Types of Statistics Descriptive and Inferential StatisticsDr. Amjad Ali Arain
Topic: Types of Statistics Descriptive and Inferential Statistics
Student Name: Bushra
Class: B.Ed. 2.5
Project Name: “Young Teachers' Professional Development (TPD)"
"Project Founder: Prof. Dr. Amjad Ali Arain
Faculty of Education, University of Sindh, Pakistan
Measure of dispersion has two types Absolute measure and Graphical measure. There are other different types in there.
In this slide the discussed points are:
1. Dispersion & it's types
2. Definition
3. Use
4. Merits
5. Demerits
6. Formula & math
7. Graph and pictures
8. Real life application.
Lecture on Introduction to Descriptive Statistics - Part 1 and Part 2. These slides were presented during a lecture at the Colombo Institute of Research and Psychology.
This presentation is on Measurement and it's scales. There are four different types of scales of measurement, namely, Nominal, Ordinal, Interval and Ratio
Types of Statistics Descriptive and Inferential StatisticsDr. Amjad Ali Arain
Topic: Types of Statistics Descriptive and Inferential Statistics
Student Name: Bushra
Class: B.Ed. 2.5
Project Name: “Young Teachers' Professional Development (TPD)"
"Project Founder: Prof. Dr. Amjad Ali Arain
Faculty of Education, University of Sindh, Pakistan
Measure of dispersion has two types Absolute measure and Graphical measure. There are other different types in there.
In this slide the discussed points are:
1. Dispersion & it's types
2. Definition
3. Use
4. Merits
5. Demerits
6. Formula & math
7. Graph and pictures
8. Real life application.
Lecture on Introduction to Descriptive Statistics - Part 1 and Part 2. These slides were presented during a lecture at the Colombo Institute of Research and Psychology.
This presentation is on Measurement and it's scales. There are four different types of scales of measurement, namely, Nominal, Ordinal, Interval and Ratio
measurement and scaling is an important tool of research. by following the right and suitable scale will provide an appropriate result of research.this slide show will additionally provide the statistical testing for research measurement and scale.
Measurement is the process observing and recording the observations that are collected as part of a research effort.
Process of assigning numbers to objects or observations, the level of measurement being a function of the rules under which the numbers are assigned.
“convert the basic materials of the problem to data”
Understanding the Scales of MeasurementDrShalooSaini
This Power Point Presentation has been made while referring to the research books written by eminent, renowned and expert authors as mentioned in the references section. The purpose of this Presentation is to help the research students in developing an insight about the Scales of Measurement.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
2. Measurement is assigning numbers to objects or events according to rules.
The level of measurement refers to the relationship among the values that are assigned
to the attributes for a variable.
There are typically four levels of measurement.
1
Nominal 2 Ordinal
3
Interval
4
Ratio
Levels of Measurement
3. In nominal measurement the numerical values just “name” the attribute uniquely.
No ordering of the cases is implied.
In nominal level of measurement, numbers are assigned to objects or events which can be placed into
mutually exclusive and exhaustive categories.
The term nominal means ‘to name’.
Names are given to the categories, with no assumption about relationships between them.
Nominal scales, also called categorical scales, measure categorical data.
For example- people may be classified as male-female, farmer- non-farmer etc. and numbers may be
assigned to them, say 1 for male and 2 for female. Number 1 simply indicates category.
Nominal
4. The appropriate measure of central tendency of a nominal scale is mode, and neither the mean
nor the median can be defined.
Permissible statistical operations- Frequency, percentage, mode, chi-square test etc.
5. In ordinal measurement the attributes can be rank-ordered.
Here, distances between attributes do not have any meaning.
Ordinal scales are those that measure rank-ordered data, such as the ranking of students in a class as first, second,
third, and so forth, based on their grade point average or test scores.
The actual or relative values of attributes or difference in attribute values cannot be assessed.
In ordinal level of measurement, numbers are assigned to objects or events which can be placed
into mutually exclusive categories and be ordered into a greater or less than scale.
At the ordinal level of measurement, numbers are assigned to objects or events not only to categorize
them but also to indicate a greater than or less than relationship.
The scale has no absolute zero point and there are unequal distances between scale values.
Numbers assigned at the ordinal level provides more information than at the nominal level because
they also establish an ordering of the objects or events.
Ordinal
6. For example, Various television programs may be categorized according to popularity.
Classification of upper, middle and lower classes in the socio- economic status.
Categories may be made on the basis of land holding.
The central tendency measure of an ordinal scale can be its median or mode, and means are uninterpretable.
Hence, statistical analyses may involve percentiles and non-parametric analysis, but more sophisticated
techniques such as correlation, regression, and analysis of variance, are not appropriate.
The most suitable statistical tests for ordinal data (e.g., Likert scale) are non-parametric tests, such as Mann-
Whitney U test (one variable, no assumption on distribution), Wilcoxon signed rank sum test (two variables,
normal distribution), Kruskal Wallis test (two or more groups, no assumption on distribution).
Statistical operations- median, percentile, correlation coefficient based on rankings etc.
7. In interval level of measurement, numbers are assigned to objects or events which can be categorized,
ordered and assumed to have an equal distance between scale values.
The zero point is set arbitrarily.
Addition and subtraction are permissible operations but because of the arbitrary zero, multiplication
and division are not permissible.
Example- measuring the temperature with thermometer, measuring the time from selecting a starting
moment, measuring the altitude from mean sea level etc.
In interval measurement the distance between attributes does have meaning.
For example, when we measure temperature (in Fahrenheit), the distance from 30-40 is same as distance
from 70-80. The interval between values is interpretable.
But in interval measurement ratios don’t make any sense - 80 degrees is not twice as hot as 40 degrees
(although the attribute value is twice as large).
Interval
8. Interval scale allows us to examine “how much more” is one attribute when compared to another,
which is not possible with nominal or ordinal scales.
Permissible statistical operations are mean, range, standard deviation, Pearson product moment correlation,
T test, F- test etc.
Permissible statistical analyses include all of those allowed for nominal and ordinal scales, plus
correlation, regression, analysis of variance, and so on.
9. In ratio level of measurement, numbers are assigned to objects or events which can be categorized, ordered,
assumed to have equals intervals between scale points and have a real zero point.
It is highest level of measurement and has all the properties of nominal, ordinal and interval plus an
absolute or true zero point.
These scales are called “ratio” scales because the ratios of two points on these
measures are meaningful and interpretable.
The salient feature of the ratio scale is that the ratio of any two numbers is independent of the unit of
measurement and therefore, it can meaningfully be equated.
Ratio scale represents the actual amounts of variables.
Ratio
10. Example- Adoption quotient
Measures of physical dimensions such as height, weight, distance etc. are examples.
Generally all statistical techniques, multiplication and division can be used with the scale.
Permissible statistical operations- central tendency and coefficient of variation.
11. Scale Central Tendency Statistics
Nominal Mode Chi-Square
Ordinal Median Percentile, Non
parametric statistics
Interval Arithmetic mean, Range,
Standard deviation
Correlation, regression,
analysis of variance
Ratio Geometric mean, Harmonic
mean
Coefficient of variation
12. Social Science Research- Principles, methods, and practices by Anol Bhattacherjee
Foundations of behavioral research- Kerlinger F. N.
Extension in Research- John. W. Best
References