This document describes a study that used bioluminescent imaging to track Streptococcus pyogenes (Group A Streptococcus) infection in the nasopharynx of mice over time. Researchers genetically engineered S. pyogenes to express the lux operon from Photorhabdus luminescens, allowing non-invasive imaging of bacteria in live mice using an IVIS imaging system. This revealed that bioluminescent S. pyogenes was able to colonize the nasopharynx and provided a non-invasive way to longitudinally track bacterial infection and clearance following vaccination over multiple time points.