Presenting our research on molecular changes in flower and extract. A visual guide through the production pipeline. With added data sets for better insight.
Optimization of the Decarboxylation Reaction in Cannabis ExtractMarkus Roggen
Presentation at Emerald Scientific Conference 2019. We present our work on building an in-process analytical method for cannabinoid quantification by IR for cannabis extract to track decarboxylation. Finding the endpoint is important for process efficiency, but surprisingly difficult. Therefore having a reliable quick and cheap tool to do so is of high value.
Cannabis/Hemp Decarboxylation Monitoring by IRMarkus Roggen
CBDV is a research venture focused on supporting the cannabis and mushroom industries through collaborative research. Some areas of research include process design, optimization, and analytics. CBDV utilizes various analytical techniques like infrared spectroscopy to monitor decarboxylation reactions for cannabis extracts. IR spectroscopy allows for fast, easy, and economical in-process monitoring of decarboxylation without sample preparation. CBDV has developed IR monitoring methods for crude oil and flower and shown IR can predict cannabinoid concentrations during decarboxylation. Computational studies explored rate differences in THCA and CBDA decarboxylation. In summary, CBDV utilizes IR spectroscopy to monitor and control decarboxylation, a key processing step.
Data Analytics for Process OptimizationMarkus Roggen
Understanding the cannabis production processes is key to improve them. We present our data analytics and optimization principles for cannabis extraction and processing.
Cannabis & Hemp Science Seminar Series - VancouverMarkus Roggen
Controlling Cannabinoids and Terpenes in Flower and Extract
Our latest research results on drying, curing and extraction of cannabis to control the chemical composition of the plant and its extracts.
CannMed 2018: Controlling Terpenes and Cannabinoids in Flower and ExtractMarkus Roggen
This document summarizes a presentation about controlling terpenes and cannabinoids in cannabis flowers and extracts. It discusses how cultivation conditions, drying/curing methods, extraction techniques, and post-processing can influence terpene and cannabinoid levels and ratios. It provides an example of how using THC from different cultivars in a CBD/THC oil caused a patient's seizures to resume, demonstrating the importance of chemovars for medical effects. The presenter advocates for research collaborations to better understand these complex relationships.
This document summarizes information from a presentation on cannabis extraction. It discusses defining extracts and what constitutes an extract in terms of cannabis compounds. It also examines optimizing extraction processes by exploring factors like temperature, pressure, time and their effects on yield, cannabinoid concentration and terpene content. The document compares subcritical and supercritical extraction methods and shares lessons learned from experiments. It also briefly discusses other separation techniques like rosin presses and ice water hash.
Optimization of the Decarboxylation Reaction in Cannabis ExtractMarkus Roggen
Presentation at Emerald Scientific Conference 2019. We present our work on building an in-process analytical method for cannabinoid quantification by IR for cannabis extract to track decarboxylation. Finding the endpoint is important for process efficiency, but surprisingly difficult. Therefore having a reliable quick and cheap tool to do so is of high value.
Cannabis/Hemp Decarboxylation Monitoring by IRMarkus Roggen
CBDV is a research venture focused on supporting the cannabis and mushroom industries through collaborative research. Some areas of research include process design, optimization, and analytics. CBDV utilizes various analytical techniques like infrared spectroscopy to monitor decarboxylation reactions for cannabis extracts. IR spectroscopy allows for fast, easy, and economical in-process monitoring of decarboxylation without sample preparation. CBDV has developed IR monitoring methods for crude oil and flower and shown IR can predict cannabinoid concentrations during decarboxylation. Computational studies explored rate differences in THCA and CBDA decarboxylation. In summary, CBDV utilizes IR spectroscopy to monitor and control decarboxylation, a key processing step.
Data Analytics for Process OptimizationMarkus Roggen
Understanding the cannabis production processes is key to improve them. We present our data analytics and optimization principles for cannabis extraction and processing.
Cannabis & Hemp Science Seminar Series - VancouverMarkus Roggen
Controlling Cannabinoids and Terpenes in Flower and Extract
Our latest research results on drying, curing and extraction of cannabis to control the chemical composition of the plant and its extracts.
CannMed 2018: Controlling Terpenes and Cannabinoids in Flower and ExtractMarkus Roggen
This document summarizes a presentation about controlling terpenes and cannabinoids in cannabis flowers and extracts. It discusses how cultivation conditions, drying/curing methods, extraction techniques, and post-processing can influence terpene and cannabinoid levels and ratios. It provides an example of how using THC from different cultivars in a CBD/THC oil caused a patient's seizures to resume, demonstrating the importance of chemovars for medical effects. The presenter advocates for research collaborations to better understand these complex relationships.
This document summarizes information from a presentation on cannabis extraction. It discusses defining extracts and what constitutes an extract in terms of cannabis compounds. It also examines optimizing extraction processes by exploring factors like temperature, pressure, time and their effects on yield, cannabinoid concentration and terpene content. The document compares subcritical and supercritical extraction methods and shares lessons learned from experiments. It also briefly discusses other separation techniques like rosin presses and ice water hash.
The science and economics of CO2 extraction and oil manufacturingMarkus Roggen
This document discusses optimization of cannabis extraction processes. It describes different extraction methods and compounds that can be extracted from cannabis, including cannabinoids and terpenes. It then discusses using design of experiments and chemometrics to optimize extraction parameters like temperature, pressure, time and particle size using supercritical CO2 extraction. Graphs show results of experiments measuring effects of time and particle size on extraction yield. The document concludes with future plans to study additional factors and scale up the extraction system.
1) CBDV is a research venture that seeks to add scientific insight to cannabis and mushroom production through process design, optimization, analytics, and formulation research conducted in collaboration with academic and industry partners.
2) CBDV's research topics include chemometrics, kinetics, in-process analytics, computational studies, and process development for fundamental cannabis and mushroom chemistry.
3) CBDV has expertise in analytical chemistry, process chemistry, engineering, physics, data science, and statistics and is led by Dr. Markus Roggen and Prof. Glenn Sammis to provide scientific insights and solutions for the cannabis and mushroom industries.
Cannabis Chemistry Industrial Applications of Chemistry & Innovation and Entr...Markus Roggen
Lecture at the chemistry department of Imperial College, London. A brought overview and specific deep dive into the chemical aspects of cannabis production.
Don't Hold Your Breath! Cannabis Labs Virtual 2021Markus Roggen
CBDV is a research venture seeking to add scientific insights to cannabis and mushroom production through collaborative research on process design, optimization, and analytics. The research focuses on topics like chemometrics, kinetic studies, in-process analytics, and computational studies to understand fundamental cannabis and mushroom chemistry. Studies have found that smoking produces higher levels of toxic thermal degradation products than vaping, and that consumption method, voltage level, and oil weight can impact toxicant levels and THC delivery.
MJBiz Con: Leading Issues in Hemp ScienceMarkus Roggen
This document summarizes hemp processing techniques including extraction methods using solvents like CO2 and ethanol. It discusses post-processing steps like decarboxylation to convert acids to neutrals, distillation to separate compounds by boiling point, chromatography to separate mixtures, and crystallization to produce pure CBD isolate. The genetic differences between hemp and cannabis are also covered as well as how regulations define hemp based on THC content.
Dr. Markus Roggen founded Complex Biotech Discovery Ventures (CBDV) to establish a research hub focused on fundamental scientific insights into cannabis production processes like decarboxylation and crystallization through collaborative projects with academic and industry partners utilizing analytical techniques like infrared spectroscopy and computational studies. CBDV seeks to optimize cannabis processing through in-line monitoring, design of experiments, data analytics, and developing a metabolite database to support the industry.
CBDV is a research venture seeking to develop analytical methods like HPLC to quantify psilocybin in psychedelic mushrooms for cultivation sites, as current methods take too long; they developed a 3.5 minute HPLC method using ammonium bicarbonate and acetonitrile to quantify psilocybin without degradation, and will next explore extraction methods from mushrooms.
Don’t hold your breath: Smoke and vaping studies on cannabis products to qua...Markus Roggen
Delic Labs is a research company that collaborates with academic and industry partners to study cannabis and mushroom production through process design, optimization, and analytics research. Their research focuses on understanding thermal degradation of cannabinoids and terpenes during smoking and vaping, as well as quantifying cannabinoid and terpene levels inhaled on a puff-by-puff basis to provide insights into real-world usage. The research aims to add fundamental scientific insights to support the cannabis and mushroom industries.
DELIC Labs seeks to add scientific insight to cannabis and mushroom production through collaborative research. Their research focuses on process design, optimization, analytics, and formulation. They collaborate with academic and industry partners. Their research topics include process control, kinetics, in-process analytics, computational studies, and process development. CO2 extraction optimization is limited by focusing on single parameters and empirical exploration. DELIC Labs uses over 100,000 data points from multiple instruments and producers to develop machine learning and Bayesian optimization models to identify optimal extraction conditions considering multiple interactive factors.
What is in your vape?! CannMed 2019 PresentationMarkus Roggen
The current research on cannabis smoking and vaping. How much THC is inhaled, which compounds are formed through heating and combustion, and how is this research done.
Cannabis Analysis Identification and Quantification of THC and CBD by GC/FID ...PerkinElmer, Inc.
Analysis of cannabis has taken on new importance in light of legalized marijuana in several states of the USA. Cannabis contains several different components classed as cannabinoids. Primary cannabinoids of interest are tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN). Positive identification and quantification of the THC/CBD ratio is a primary objective in the analysis of cannabis. Cannabis is analyzed for several different purposes. This application note will concentrate on the potency identification and quantification of THC and CBD in cannabis by Gas Chromatography.
This report from Botanica Testing Inc. summarizes the analysis of a CBD isolate sample. The report provides the batch number, container type, label information, total cannabinoid content in mg/g, individual cannabinoid percentages, and signatures from the CEO and Chief Scientific Officer. The results indicate the sample is 99.12% CBD and 0.00% THC.
Controlling Terpenes and Cannabinoids in Flower & ExtractBlake Grauerholz
This talk encompasses all of our recent work from 2016-2017. Methods for optimizing & preserving terpene and cannabinoid composition is a primary focus covering all aspects from cultivation, harvest, extraction, to packaging of finished products.
Crystallizing the science of CBD purificationMarkus Roggen
Cannabidiol, or CBD, is the new star of functional ingredients. It is a food, medicine, saviour of the farmers, even a health elixir.
The cannabis and hemp industries have recently experienced extreme growth and progress in all fields. Countries, states and countries are legalizing hemp and/or cannabis, cannabinoid producers see unpreceded business growth, new treatment option for various conditions are researched and approved. But while the markets are growing, hemp and cannabis producers are suffering. An overproduction depresses commodity prices, and inefficient processes squeeze the profit margins. Additionally, hemp and cannabis products require high purity of cannabinoid ingredients, both from a regulatory level and for patient care.
This presentation will cover the latest research on CBD isolation, from its solubility behaviour in common extraction solvents to purification via crystallization. Our latest findings in understanding, controlling, and optimizing cannabis production in analytics, processing, extraction and formulation are presented. The collaborative effort of the multidisciplinary research team at Delic Labs led to a diverse set of insight in every stage of production.
This poster shows an HPLC method which builds on the well-established potency method using the Shimadzu Cannabis Analyzer for Potency™, a comprehensive and fast determination of 21 cannabinoids in only 15 minutes (including the wash-step). Cannabinoid profiles for commercially available dry hemp and finished tinctures are presented.
MACHEREY-NAGEL is a globally operating company with stable growth. In the recent years, the annual turnover exceeded 100 million Euros.
Our comprehensive portfolio includes products for Filtration, Rapid Tests, Water Analysis, Chromatography, and Bioanalysis. We are proud to carry more than 20,000 products designed and manufactured to fit your individual needs.
With more than 470 highly qualified and experienced employees MACHEREY-NAGEL provides the best and most convenient service to our customers. 10 % of our staff have advanced degrees in the fields of Chemistry, Biology, Physics and Engineering, working in our research department on solutions to make your daily laboratory work easier.
The science and economics of CO2 extraction and oil manufacturingMarkus Roggen
This document discusses optimization of cannabis extraction processes. It describes different extraction methods and compounds that can be extracted from cannabis, including cannabinoids and terpenes. It then discusses using design of experiments and chemometrics to optimize extraction parameters like temperature, pressure, time and particle size using supercritical CO2 extraction. Graphs show results of experiments measuring effects of time and particle size on extraction yield. The document concludes with future plans to study additional factors and scale up the extraction system.
1) CBDV is a research venture that seeks to add scientific insight to cannabis and mushroom production through process design, optimization, analytics, and formulation research conducted in collaboration with academic and industry partners.
2) CBDV's research topics include chemometrics, kinetics, in-process analytics, computational studies, and process development for fundamental cannabis and mushroom chemistry.
3) CBDV has expertise in analytical chemistry, process chemistry, engineering, physics, data science, and statistics and is led by Dr. Markus Roggen and Prof. Glenn Sammis to provide scientific insights and solutions for the cannabis and mushroom industries.
Cannabis Chemistry Industrial Applications of Chemistry & Innovation and Entr...Markus Roggen
Lecture at the chemistry department of Imperial College, London. A brought overview and specific deep dive into the chemical aspects of cannabis production.
Don't Hold Your Breath! Cannabis Labs Virtual 2021Markus Roggen
CBDV is a research venture seeking to add scientific insights to cannabis and mushroom production through collaborative research on process design, optimization, and analytics. The research focuses on topics like chemometrics, kinetic studies, in-process analytics, and computational studies to understand fundamental cannabis and mushroom chemistry. Studies have found that smoking produces higher levels of toxic thermal degradation products than vaping, and that consumption method, voltage level, and oil weight can impact toxicant levels and THC delivery.
MJBiz Con: Leading Issues in Hemp ScienceMarkus Roggen
This document summarizes hemp processing techniques including extraction methods using solvents like CO2 and ethanol. It discusses post-processing steps like decarboxylation to convert acids to neutrals, distillation to separate compounds by boiling point, chromatography to separate mixtures, and crystallization to produce pure CBD isolate. The genetic differences between hemp and cannabis are also covered as well as how regulations define hemp based on THC content.
Dr. Markus Roggen founded Complex Biotech Discovery Ventures (CBDV) to establish a research hub focused on fundamental scientific insights into cannabis production processes like decarboxylation and crystallization through collaborative projects with academic and industry partners utilizing analytical techniques like infrared spectroscopy and computational studies. CBDV seeks to optimize cannabis processing through in-line monitoring, design of experiments, data analytics, and developing a metabolite database to support the industry.
CBDV is a research venture seeking to develop analytical methods like HPLC to quantify psilocybin in psychedelic mushrooms for cultivation sites, as current methods take too long; they developed a 3.5 minute HPLC method using ammonium bicarbonate and acetonitrile to quantify psilocybin without degradation, and will next explore extraction methods from mushrooms.
Don’t hold your breath: Smoke and vaping studies on cannabis products to qua...Markus Roggen
Delic Labs is a research company that collaborates with academic and industry partners to study cannabis and mushroom production through process design, optimization, and analytics research. Their research focuses on understanding thermal degradation of cannabinoids and terpenes during smoking and vaping, as well as quantifying cannabinoid and terpene levels inhaled on a puff-by-puff basis to provide insights into real-world usage. The research aims to add fundamental scientific insights to support the cannabis and mushroom industries.
DELIC Labs seeks to add scientific insight to cannabis and mushroom production through collaborative research. Their research focuses on process design, optimization, analytics, and formulation. They collaborate with academic and industry partners. Their research topics include process control, kinetics, in-process analytics, computational studies, and process development. CO2 extraction optimization is limited by focusing on single parameters and empirical exploration. DELIC Labs uses over 100,000 data points from multiple instruments and producers to develop machine learning and Bayesian optimization models to identify optimal extraction conditions considering multiple interactive factors.
What is in your vape?! CannMed 2019 PresentationMarkus Roggen
The current research on cannabis smoking and vaping. How much THC is inhaled, which compounds are formed through heating and combustion, and how is this research done.
Cannabis Analysis Identification and Quantification of THC and CBD by GC/FID ...PerkinElmer, Inc.
Analysis of cannabis has taken on new importance in light of legalized marijuana in several states of the USA. Cannabis contains several different components classed as cannabinoids. Primary cannabinoids of interest are tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN). Positive identification and quantification of the THC/CBD ratio is a primary objective in the analysis of cannabis. Cannabis is analyzed for several different purposes. This application note will concentrate on the potency identification and quantification of THC and CBD in cannabis by Gas Chromatography.
This report from Botanica Testing Inc. summarizes the analysis of a CBD isolate sample. The report provides the batch number, container type, label information, total cannabinoid content in mg/g, individual cannabinoid percentages, and signatures from the CEO and Chief Scientific Officer. The results indicate the sample is 99.12% CBD and 0.00% THC.
Controlling Terpenes and Cannabinoids in Flower & ExtractBlake Grauerholz
This talk encompasses all of our recent work from 2016-2017. Methods for optimizing & preserving terpene and cannabinoid composition is a primary focus covering all aspects from cultivation, harvest, extraction, to packaging of finished products.
Crystallizing the science of CBD purificationMarkus Roggen
Cannabidiol, or CBD, is the new star of functional ingredients. It is a food, medicine, saviour of the farmers, even a health elixir.
The cannabis and hemp industries have recently experienced extreme growth and progress in all fields. Countries, states and countries are legalizing hemp and/or cannabis, cannabinoid producers see unpreceded business growth, new treatment option for various conditions are researched and approved. But while the markets are growing, hemp and cannabis producers are suffering. An overproduction depresses commodity prices, and inefficient processes squeeze the profit margins. Additionally, hemp and cannabis products require high purity of cannabinoid ingredients, both from a regulatory level and for patient care.
This presentation will cover the latest research on CBD isolation, from its solubility behaviour in common extraction solvents to purification via crystallization. Our latest findings in understanding, controlling, and optimizing cannabis production in analytics, processing, extraction and formulation are presented. The collaborative effort of the multidisciplinary research team at Delic Labs led to a diverse set of insight in every stage of production.
This poster shows an HPLC method which builds on the well-established potency method using the Shimadzu Cannabis Analyzer for Potency™, a comprehensive and fast determination of 21 cannabinoids in only 15 minutes (including the wash-step). Cannabinoid profiles for commercially available dry hemp and finished tinctures are presented.
MACHEREY-NAGEL is a globally operating company with stable growth. In the recent years, the annual turnover exceeded 100 million Euros.
Our comprehensive portfolio includes products for Filtration, Rapid Tests, Water Analysis, Chromatography, and Bioanalysis. We are proud to carry more than 20,000 products designed and manufactured to fit your individual needs.
With more than 470 highly qualified and experienced employees MACHEREY-NAGEL provides the best and most convenient service to our customers. 10 % of our staff have advanced degrees in the fields of Chemistry, Biology, Physics and Engineering, working in our research department on solutions to make your daily laboratory work easier.
Throughout history, cannabis has been used as a panacea, an herbal remedy for nearly all medical concerns from simple headaches to severe pain. Now that many states have legalized medical cannabis, it is important to have analytical methodologies to study the compounds that the patients will be ingesting or inhaling. Terpenes are a major class of compounds found in cannabis. They are volatile hydrocarbons responsible for the plant’s aroma. These compounds are found in other plants as well. Through various clinical trials they were found to be medically relevant. In terms of cannabis, these compounds reportedly assist the cannabinoids in their effects. The cannabinoids bind to the cannabinoid receptor in the brain, and thus have medical relevance. Cannabichromene, cannabidiol, cannabigerol, and cannabinol are the main four cannabinoids that are implicated in relieving symptoms of pain, nausea, and directly reducing seizures. Delta-9-tetrahydrocannabinol is responsible for the euphoria experienced when smoked or ingested.
With the increase in usage of cannabis due to its medical legalization in many states, it is important to have analytical methods for testing potency and variance of the cannabinoids and terpenes within the plant material. To do this, terpenes and cannabinoids were analyzed using a GC-FID. As the terpenes have higher volatility, several injection techniques were tested, including liquid injection, SPME, and headspace. The cannabinoid method was then applied to test the variance in subsequent doses of the same size, mimicking that of doses distributed to patients.
Determination of dicarbonyls and crotonaldehyde in e-vapor productsRana Tayyarah
A robust method for ecigarette FDA-CTP PMTA guidance analytes: Diacetyl, Acetyl propionyl (also known as 2,3-pentanedione), and crotonaldehyde
2019_TSRC14_Zich.pdf
This study focuses on particle sizing and the potential effects it has on the molecular makeup of milled cannabis, extract quality and efficiency in SFE.
This document discusses safety compliance in the cannabis industry and recalls due to pesticide contamination. It provides the following key points:
- Safety compliance facilities are the first to get licensed and ensure safety standards are met through testing. However, some have faced recalls due to pesticide contamination from suppliers.
- Several cannabis businesses in Colorado and Oregon have had recalls involving thousands of units due to failing pesticide tests. Traces of unauthorized pesticides have been found, some as low as 5 parts per billion.
- Colorado saw 49% of cannabis samples test positive for unauthorized pesticides in 2016 and 13% so far in 2017. Oregon found pesticide failures in hundreds of samples last year.
- Cannabis testing
Total Legal: A “Joint” Journey into the Chemistry of CannabinoidsMarkus Roggen
This lecture gives an overview of the state of the cannabis science, the North American markets and our cannabis research.
Bridging the gap between analytical chemistry, machine learning, and synthetic chemistry, this compilation of studies explores the multifaceted nature of cannabis compounds. We begin with advancements in extraction techniques, utilizing machine learning to optimize yield predictions in large-scale botanical recovery, specifically focusing on cannabis. This approach significantly enhances the efficiency and precision of extraction processes. We then transitions to molecular analysis, examining the stability and transformation of CBD derivatives, highlighting the need for robust quality control in product development. Additionally, the lecture addresses the critical aspect of safety in cannabis use by exploring innovative strategies for heavy metal testing, demonstrating how pooling methods can reduce testing resources while maintaining safety standards. The lecture will answer the question of how to build the best joint, the implications of joint architecture on cannabis consumption, offering insights into optimizing product efficacy and consumer experience.
Overall, this lecture encapsulates the collaborative efforts between academia and innovative research, providing a comprehensive understanding of the complexities and potential of cannabis chemistry.
Birds, Bees and Buds: How to Talk About Cannabis and How to Label it CorrectlyMarkus Roggen
The current labeling of cannabis products is complicated, useless, and even gives the wrong incentive to the customer. The current market is tilted towards buying the highest total THC percentage, because it is a number that everyone believes they understand. There is a lot wrong with this. Starting from the problem that total THC is miscalculated. Adding the fact that “potency” and intoxication are not directly linked. Cannabis offers more bioactive compounds then just THC and CBD, but current labeling doesn’t show it. How can we present a cannabis product in a new way to guide the customer/patent to the right product. This presentation will build on my publications in the area of total THC labeling and chemovar research.
Phytochemical investigation of lawsonia inermis linn and itsMamoona Nigha
This document summarizes a study investigating the phytochemical components and applications of Lawsonia inermis Linn (henna) leaves. The aims were to evaluate the antioxidant and antimicrobial properties of the essential oil and extracts. Methods included extracting and analyzing the essential oil using GC-MS, performing phytochemical analysis of extracts, and testing antioxidant activity and color formulations. Results found the essential oil contained compounds like α-pinene and exhibited antioxidant and antimicrobial effects. Phytochemical analysis revealed the presence of alkaloids, flavonoids, and other compounds. Formulations with mordants were made and tested on leather, showing variations in properties. In conclusion, the study characterized components of he
This document summarizes a study on optimizing the extraction of sandalwood oil using subcritical carbon dioxide compared to conventional techniques like steam distillation. Subcritical carbon dioxide extraction at 200 bars and 28°C yielded 4.11% oil in the first hour, higher than other methods and with higher quality as indicated by acid value and santalol content. Analysis found the oil extracted in each hour contained varying amounts of key constituents like α-santalene and β-santalol. The study demonstrates that subcritical carbon dioxide extraction is more efficient and yields higher quality sandalwood oil than conventional techniques like steam distillation.
Hot Topics and Hotboxing: Latest research on cannabis aerosolsMarkus Roggen
Cannabis testing focuses on harm reduction, by testing for unwanted pesticides, heavy metals and biological contamination. On one side, cannabinoid levels are precisely measured in the product.
Although these final product tests miss one important aspect of cannabis consumption. The actual process of consumption. For inhalable products, like joints and vape cartridges, the dosing of cannabinoids and chemical changes during burning/vaporization are mostly unknow.
We have developed a testing platform to quantify cannabinoid and terpene levels on a puff per puff basis. This setup also allows us to screen the aerosol and gas phase for potential toxicants present. We also looked at cannabinoid concentrations puff by puff, how those compare between joints and vapes, and how those levels change over the lifecycle of a product.
In this talk we will present our findings from our latest vape and joint experiment and answer the longstanding question: Which part of a joint is the best to smoke? And, will cannabis kill you?
The presentation describes the automated process of the system and present a number of applications from sample matrices such as food, polymers, and pharmaceuticals to show the utility of the system.
Supercritical fluid extraction of food components in food industries such as ...CaresmaChuwa
Supercritical fluid is a Fluid which is in the state above its critical point (CP), i.e. above its critical temperature (Tc) and critical pressure (Pc) where distinct liquid and gas phases do not exist.
It can be produced by warming a gas or liquid on temperature higher than its critical temperature Tc by simultaneous compression on value higher than its Pc
Above the critical temperature and pressure is the material in one condensed state with properties between gas and liquid
The document summarizes a study that analyzed the effects of storage conditions on the volatile profile of lemon essential oil microcapsules produced by combining membrane emulsification and spray-drying. Lemon microcapsules were stored at 5°C and 40°C for 180 days. Selected volatile compounds were analyzed by solid phase microextraction and gas chromatography. Storage at 40°C had a more pronounced influence on the oxidation product alpha-pinene and on alpha-terpineol, acetyl nerol, and acetyl geraniol contents. Linalool did not suffer significant changes during storage regardless of temperature or microcapsule composition. Microcapsules produced using Arabic gum showed less severe oxidation of lemon oil and alpha-pinene production compared
Supercritical Fluid Extraction in Food AnalysisVarad Bende
Supercritical Fluid Extraction (SFE) has emerged as a promising technique of extraction in past few years in food domain. The presentation reviews the theoretical aspects, instrumentation, applications and some case studies.Supercritical Fluid Extraction (SFE) has emerged as a promising technique of extraction in past few years in food domain. The presentation reviews the theoretical aspects, instrumentation, applications and some case studies.
Introducing the SPExtR Extraction System.pptxSimplebiz1
The document introduces the SPExtR extraction system from Myers Vacuum, a new solventless approach to cannabis extraction. Plant material is weighed and loaded into the system, parameters are set, and the process extracts three portions - spent plant material, a decarbed cannabis-rich portion, and a terpene-rich portion. Test results show the system producing extracts with over 77% THC and over 80% total cannabinoids, demonstrating high potency without using any solvents, steam, CO2, hot gases, or water.
comparison of colour and capsaicin content in different type of chilli using ...aswathibabuachus
The document describes a study that compares the color value and capsaicin content of different types of chili using spectrophotometric methods. Five varieties of chili were tested: Wonder Hot, Red Top, White, Green, and Teja Red. The results showed that White chili had the highest capsaicin content at 9.3695% while Teja Red chili had both a high color value and capsaicin content, suggesting it is the best quality variety. Spectrophotometric analysis was used to measure color value and capsaicin content.
Similar to LabRoots 2019: Molecular Manipulation (20)
Unveiling the Cannabis Plant’s PotentialMarkus Roggen
Cannabinoid research is one of the most exciting areas of the cannabis science field, with new information being discovered about the potential therapeutic benefits of cannabinoids on a seemingly frequent basis. As we learn more about the cannabinoids that make up the cannabis plant and their unique qualities, research must be conducted with a focus on applying knowledge toward developing wellness products and cannabis therapies for patients. This panel will discuss the unique challenges facing the cannabinoid research field, results from recent research efforts, and what the future may hold for therapeutic applications of cannabinoids.
Have a Good Trip? How to Analyze PsychedelicsMarkus Roggen
Entheogens, frequently used interchangeably with hallucinogens and psychedelics, are naturally occurring psychoactive substances. Historically, these substances have been used to induce a change of perception, mood, consciousness, or behavior for the purposes of spiritual development or social enrichment. In addition to these uses, these substances have become strong candidates for alternative medicines for the treatment of psychological disorders. Cannabis, a more common entheogen, has paved the way for decriminalization and legalization of other substances, such as psilocin, psilocybin, MDMA, and LSD to be used for clinical treatments. While these compounds in many places are still classified as Schedule I substances under the Controlled Substances Act, other entheogenic plants with different psychoactive compounds are not, such as Mitragyna speciosa and Amanita muscaria, which already appear on the market in the form of edibles, extracts, or powders. With the lack of standardized methods for these psychoactive targets, and with the rise of legalization and clinical treatment centers, it is important to understand this emerging market from a safety and quality perspective. This session will focus on emerging products, testing targets, sample preparation, method development, and analytical challenges. Attendees of this session will learn technical steps that need to be considered to develop robust, reproducible analytical methods, and a quality assurance program to expand into this new and evolving area of testing.
From Leaf to Lab: Uncovering the Molecular Mysteries of CannabisMarkus Roggen
This lecture presents our cannabis research, conducted in collaboration with the University of British Columbia (UBC) Chemistry Department.
Bridging the gap between analytical chemistry, machine learning, and synthetic chemistry, this compilation of studies explores the multifaceted nature of cannabis compounds. We begin with advancements in extraction techniques, utilizing machine learning to optimize yield predictions in large-scale botanical recovery, specifically focusing on cannabis. This approach significantly enhances the efficiency and precision of extraction processes. We then transitions to molecular analysis, examining the stability and transformation of CBD derivatives, highlighting the need for robust quality control in product development. Additionally, the lecture addresses the critical aspect of safety in cannabis use by exploring innovative strategies for heavy metal testing, demonstrating how pooling methods can reduce testing resources while maintaining safety standards. The lecture will answer the question of how to build the best joint, the implications of joint architecture on cannabis consumption, offering insights into optimizing product efficacy and consumer experience.
Overall, this lecture encapsulates the collaborative efforts between academia and innovative research, providing a comprehensive understanding of the complexities and potential of cannabis chemistry.
Your Secret Sauce to Succeeding as a Cannabis Business: A Data-Driven ApproachMarkus Roggen
The cannabis industry is going through boom-bust cycles, many businesses are losing money or barely scrape by. All that is set against an ever-growing market with sales growing 20-30% year over year.
In this session we are analysing various factors exerting pressure on the industry, from taxes to testing costs, but also business strategies and operational decisions. This is all funded on extensive analysis of dataset from sales, business performance, production processes and public sources.
The work from an interdisciplinary team of economists, data scientists and chemists cumulated in an in-depth analysis of the cannabis economy and guiding principles for industry-wide success.
How Long Is Your Trip? Analysing the Micros and Heroics of PsychedelicsMarkus Roggen
Psychedelics are a diverse group of drugs that are known for their ability to alter consciousness, perception, mood, and thought. Detecting the presence, quantity and quality of these compounds is crucial to research development and involves various analytical tools such as High Performance Liquid Chromatography (HPLC) with optical or mass detectors, and other instruments types. These analytical tests are performed for a variety of reasons, including product, drug, or safety testing, all of which are subject to regulations and guidelines set by the licensing authorities. Besides the regulations, we face several other challenges with psychedelic analysis, such as the lack of standardized testing methods, difficulties in sample preparation, and analyte stability.
Simulations of Test Reduction Using Pooled Heavy Metals Analysis in CannabisMarkus Roggen
Background: Cannabis species have a propensity to bioaccumulate toxic heavy metals from their growth media. Increased testing for these metals is required to improve the safety of the legal medical and recreational cannabis industries. However, the current methods used for mandated heavy metals tests are not efficient for a large framework. As a result, there is limited testing capacity, high testing costs, and long wait times for results across North America.
Objective: This study aimed to demonstrate that pooling strategies can be used to increase the throughput in cannabis testing labs and reduce some of the strain on the industry.
Methods: This paper presents an algorithm to simulate different pooling strategies. The algorithm was applied to real world data sets collected from Washington and California state testing labs.
Results: Using a single pooling method, for the California lab, pooling four samples on average resulted in a 54.1% reduction in tests required for 100 samples. Conclusion: The algorithms generated from lab data demonstrated that pooled testing strategies can be developed on a case- by-case method to reduce the time, effort, and costs associated with heavy metals tests.
Highlights: The benefits of pooled testing will vary depending on the region and rate of contamination seen in each testing lab. Overall, our results demonstrate pooled testing has the potential to reduce the fiscal costs of testing through increased efficiency, allowing increased testing, leading to greater safety.
Structure Activity Relationship between the Emerging Psychedelic Industry and...Markus Roggen
DELIC Labs seeks to establish a research hub in Vancouver to study cannabis and mushrooms through process design, optimization, analytics, and formulation research. It collaborates with academic and industry partners globally. The document discusses psychedelic compounds and their medical potential but also highlights flaws in current clinical trials, a lack of knowledge about psilocybin mushroom composition, and Delic Labs' work using metabolomics to address this. It also covers the patenting of psilocybin crystal structures and questions whether this is appropriate given the medical applications of these compounds.
- DELIC Labs conducts research on cannabis and mushroom production, collaborating with academic and industry partners. Research topics include process optimization, analytics, and formulation studies.
- CBD usage is increasing globally but users face confusion over legality, regulation, and determining proper dosages. CBD concentrations in e-liquids were found to deviate significantly from labeled amounts in some cases.
- CBD is subject to degradation over time from factors like heat, light, pH levels, and metal leaching. On average, 20% degradation was observed after 30 days at 37°C.
Out of the Shadows: Identifying Impurities in Cannabis ProductsMarkus Roggen
Highly concentrated cannabis products have seen rapid growth, as customers become more accustomed to cannabis consumption and actively seek out high-potency products. Cannabis concentrates come in various forms and product names, from Badder, Budder and Crumble to Distillate, Oil and Shatter. Those products can have THC concentrations are high as 95%, compared with less than 25% common in cannabis flower.
While the actual THC concentration can vary between 70 and 95%, what is common for all cannabis concentrates is that the total of identified compounds seldomly goes above 95% of product weight.
Delic Labs is a research venture that seeks to add fundamental scientific insight to the field of cannabis and mushroom production. In this regard, we set out to identify those compounds in the missing mass balance for cannabis concentrates. This presentation will show our latest advances in characterizing and quantifying impurities in cannabis concentrates. For example, we found reduced cannabinoid species in CBN products, THC isomers in distillates and oxidation products in CBD formulations.
Deep Purple: Discolouration in CBD productsMarkus Roggen
Presentation at ACS Spring 2022 conference.
Recently, there has been a flood of cannabidiol (CBD)-containing products, with divers marketing claims for a plethora of use cases. With this new market segment, regulatory oversight is still developing, and label claims of CBD concentration is often verifiable. CBD is unstable in solution and some CBD products are anecdotally reported to turn purple on storage; however, the decomposition products of CBD are mostly unknown.
Delic Lab embarked on a long and painstaking hunt for those decomposition products, established their identity through complementary chemical methods and established reaction pathways between them.
We will present our findings about common CBD oxidation products, who those are highly photochemically unstable and decomposes rapidly. Decomposition leads to a multitude of new cannabinoid derivatives.
Artificial Intelligence for Craft Cannabis ProductsMarkus Roggen
Cannabis extraction operations focus on extraction yields. This is an imprecise way to think about production. Defining the yield as yield per pound of biomass is very difference to yield per hour. And yield should not be the only focus for extractors. Quality of extract, cost of extraction, loss in post-processing, all these aspects should be considered too.
Our laboratory undertook extensive experimental studies on the extraction behavior of various solvents. We furthermore analyzed thousands of real-world extractions, from various producers and for different instruments to build a machine learning algorithm that can optimize extraction processes autonomously.
We present our latest results and insights from developing and utilizing our extraction optimization AI, to give the audience most actionable insight on how to make better cannabis extracts.
This document discusses using data analytics to optimize cannabis extraction and production processes. It describes how tracking additional metrics like net weight collected, run time, and flow rate can help producers increase yields and profit margins. Specifically, a predictive algorithm could help fill data gaps. The document also discusses cannabis extraction variables that impact yields, such as temperature, pressure and run time. It suggests extraction runs have an initial mass transfer mode where longer times yield more, but eventually reach a diffusion mode with diminishing returns. Overall, the document advocates for data-driven approaches to optimize cannabis extraction and production efficiency.
Cannabis Science Conference 2019: CBDV Fundamental CollaborationMarkus Roggen
CBDV is a research venture that seeks to add fundamental scientific insight to cannabis production through collaboration. It operates a research hub in Vancouver for process design, optimization, analytics, and formulation research. CBDV collaborates with academic and industry partners globally on topics like extraction optimization, analytics for reaction monitoring, and computational studies on decarboxylation rates. The goal is to answer fundamental questions about cannabis cultivation and processing to support a mature cannabis products market.
When I was asked to give a companion lecture in support of ‘The Philosophy of Science’ (https://shorturl.at/4pUXz) I decided not to walk through the detail of the many methodologies in order of use. Instead, I chose to employ a long standing, and ongoing, scientific development as an exemplar. And so, I chose the ever evolving story of Thermodynamics as a scientific investigation at its best.
Conducted over a period of >200 years, Thermodynamics R&D, and application, benefitted from the highest levels of professionalism, collaboration, and technical thoroughness. New layers of application, methodology, and practice were made possible by the progressive advance of technology. In turn, this has seen measurement and modelling accuracy continually improved at a micro and macro level.
Perhaps most importantly, Thermodynamics rapidly became a primary tool in the advance of applied science/engineering/technology, spanning micro-tech, to aerospace and cosmology. I can think of no better a story to illustrate the breadth of scientific methodologies and applications at their best.
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...Leonel Morgado
Current descriptions of immersive learning cases are often difficult or impossible to compare. This is due to a myriad of different options on what details to include, which aspects are relevant, and on the descriptive approaches employed. Also, these aspects often combine very specific details with more general guidelines or indicate intents and rationales without clarifying their implementation. In this paper we provide a method to describe immersive learning cases that is structured to enable comparisons, yet flexible enough to allow researchers and practitioners to decide which aspects to include. This method leverages a taxonomy that classifies educational aspects at three levels (uses, practices, and strategies) and then utilizes two frameworks, the Immersive Learning Brain and the Immersion Cube, to enable a structured description and interpretation of immersive learning cases. The method is then demonstrated on a published immersive learning case on training for wind turbine maintenance using virtual reality. Applying the method results in a structured artifact, the Immersive Learning Case Sheet, that tags the case with its proximal uses, practices, and strategies, and refines the free text case description to ensure that matching details are included. This contribution is thus a case description method in support of future comparative research of immersive learning cases. We then discuss how the resulting description and interpretation can be leveraged to change immersion learning cases, by enriching them (considering low-effort changes or additions) or innovating (exploring more challenging avenues of transformation). The method holds significant promise to support better-grounded research in immersive learning.
Phenomics assisted breeding in crop improvementIshaGoswami9
As the population is increasing and will reach about 9 billion upto 2050. Also due to climate change, it is difficult to meet the food requirement of such a large population. Facing the challenges presented by resource shortages, climate
change, and increasing global population, crop yield and quality need to be improved in a sustainable way over the coming decades. Genetic improvement by breeding is the best way to increase crop productivity. With the rapid progression of functional
genomics, an increasing number of crop genomes have been sequenced and dozens of genes influencing key agronomic traits have been identified. However, current genome sequence information has not been adequately exploited for understanding
the complex characteristics of multiple gene, owing to a lack of crop phenotypic data. Efficient, automatic, and accurate technologies and platforms that can capture phenotypic data that can
be linked to genomics information for crop improvement at all growth stages have become as important as genotyping. Thus,
high-throughput phenotyping has become the major bottleneck restricting crop breeding. Plant phenomics has been defined as the high-throughput, accurate acquisition and analysis of multi-dimensional phenotypes
during crop growing stages at the organism level, including the cell, tissue, organ, individual plant, plot, and field levels. With the rapid development of novel sensors, imaging technology,
and analysis methods, numerous infrastructure platforms have been developed for phenotyping.
Current Ms word generated power point presentation covers major details about the micronuclei test. It's significance and assays to conduct it. It is used to detect the micronuclei formation inside the cells of nearly every multicellular organism. It's formation takes place during chromosomal sepration at metaphase.
Authoring a personal GPT for your research and practice: How we created the Q...Leonel Morgado
Thematic analysis in qualitative research is a time-consuming and systematic task, typically done using teams. Team members must ground their activities on common understandings of the major concepts underlying the thematic analysis, and define criteria for its development. However, conceptual misunderstandings, equivocations, and lack of adherence to criteria are challenges to the quality and speed of this process. Given the distributed and uncertain nature of this process, we wondered if the tasks in thematic analysis could be supported by readily available artificial intelligence chatbots. Our early efforts point to potential benefits: not just saving time in the coding process but better adherence to criteria and grounding, by increasing triangulation between humans and artificial intelligence. This tutorial will provide a description and demonstration of the process we followed, as two academic researchers, to develop a custom ChatGPT to assist with qualitative coding in the thematic data analysis process of immersive learning accounts in a survey of the academic literature: QUAL-E Immersive Learning Thematic Analysis Helper. In the hands-on time, participants will try out QUAL-E and develop their ideas for their own qualitative coding ChatGPT. Participants that have the paid ChatGPT Plus subscription can create a draft of their assistants. The organizers will provide course materials and slide deck that participants will be able to utilize to continue development of their custom GPT. The paid subscription to ChatGPT Plus is not required to participate in this workshop, just for trying out personal GPTs during it.
Travis Hills' Endeavors in Minnesota: Fostering Environmental and Economic Pr...Travis Hills MN
Travis Hills of Minnesota developed a method to convert waste into high-value dry fertilizer, significantly enriching soil quality. By providing farmers with a valuable resource derived from waste, Travis Hills helps enhance farm profitability while promoting environmental stewardship. Travis Hills' sustainable practices lead to cost savings and increased revenue for farmers by improving resource efficiency and reducing waste.
Remote Sensing and Computational, Evolutionary, Supercomputing, and Intellige...University of Maribor
Slides from talk:
Aleš Zamuda: Remote Sensing and Computational, Evolutionary, Supercomputing, and Intelligent Systems.
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Inter-Society Networking Panel GRSS/MTT-S/CIS Panel Session: Promoting Connection and Cooperation
https://www.etran.rs/2024/en/home-english/
1. Controlling Terpenes and
Cannabinoids in Flower and
Extract
Cannabis Curing & Extraction Research
by
Dr. Markus Roggen
Complex Biotech Discovery Ventures
2. Agenda
• Introduction
• Data Visualization
• Different Strains = Different Chemovars
• Drying & Storage
• Drying Conditions
• Gasses for Storage
• Extraction
• Particle Size
• DoE
• Post-Processing
• Decarboxylation
4. Cannabis Processing Pipeline
• Drying and curing
• Extraction
• Post processing
Myrcene
a-Pinene
b-Pinene
Limonene
b-Caryophyllene
5. Cannabis Flower and Medicine
• Cannabinoid ratios and concentrations show little variability
• Terpene ratios and concentrations show strong variability
Cannabinoid Chemotaxonomy: DOI:10.1038/s41598-018-31120-2
Terpene Chemovars: DOI: 10.1089/can.2016.0040
Gorilla Glue Sour Diesel
Cookies
Sour Tangie
Lemonade Haze
6. What is in the Plant
6
>140 Cannabinoids
>200 Terpenes
~30 Flavonoids
Alcohols, Phenols
Aldehydes, Ketones
Alkaloids
Carbohydrates, Fatty Acids
Lactones
7. Drying and Curing
• Drying room conditions influence on terpenes
30%
Drying
Conditions
Slower
Faster
Air
Vacuum
N2
Ar
Long Term
Storage
20%
CO2
Storage
Atmosphere
8. Water Activity during Dry and Cure
0.45
0.55
0.65
0.75
0.85
0.95
0 5 10 15 20 25 30
WaterActivity
Day
Water Activity
Aw of 0.65 represents the value in which
Botrytis cannot continue to grow
Terpene Content (% dry weight)
Rapid Dry Slow Dry
Lemonade Haze 1.42 1.82
Sour Tangie 1.69 2.2
• Moisture loss is driven by a difference in vapor pressure
• Balance between inhibiting mold growth and terpene loss
9. Drying and Curing
• Drying room conditions influence on terpenes
• Storage time influences on cannabinoids
• Drying room conditions influence on terpenes
• Storage time influences on cannabinoids
• Curing Gases influence on terpene ratios
30%
Drying
Conditions
Slower
Faster
Air
Vacuum
N2
Ar
Long Term
Storage
20%
CO2
Storage
Atmosphere
10. Drying and Curing
• Drying room conditions influence on terpenes
• Storage time influences on cannabinoids
• Curing Gases influence on terpene ratios
30%
Drying
Conditions
Slower
Faster
Air
Vacuum
N2
Ar
Long Term
Storage
20%
CO2
Storage
Atmosphere
11. Storing under Modified Atmosphere
18.00
20.00
22.00
24.00
26.00
28.00
30.00
0 2 4 6 8 10 12 14 16
w%
Weeks
Cannabinoids
Air Vacuum N2 Ar CO2
0
0.5
1
1.5
2
2.5
0 2 4 6 8 10 12 14 16 18
w%
Weeks
Total Terpens
Air Vacuum N2 Ar CO2
14. Effect of Milling
Does milling destroy THC?
0%
1%
2%
%decarb by Size
0
0.2
0.4
0.6
0.8
1
1.2
1.4
%wt. Terpenes by Size
15. Effect of Milling
But it does give you higher yields!
20
22
24
26
28
30
32
34
Non-Ground Food Blender 2 mm 6 mm 10 mm
%Recovery
Cannabinoid Recovery by Size
75
80
85
90
95
Non-Ground Food Blender 2 mm 6 mm 10 mm
%Recovery
Terpene Recovery by Size
16. Extraction Parameters
• 3 kg of plant matter
• Terpene Fraction (F1): 1100 psi, 34˚C, 5 h
• Cannabinoid Fraction (F2): 1900 psi, 60˚C, 12 h
17. Extraction Precision Terpenes
30
35
40
45
50
55
Non-Ground Food Blender 2 mm 6 mm 10 mm
%conc.
Terpene Concentration F1
Higher is better, Food Blender wins
0
1
2
3
4
5
Non-Ground Food Blender 2 mm 6 mm 10 mm
%conc.
Terpene Concentration F2
Lower is better, 2mm wins
• 3 kg of plant matter
• Terpene Fraction (F1): 1100 psi, 34˚C, 5 h
• Cannabinoid Fraction (F2): 1900 psi, 60˚C, 12 h
18. 0
2
4
6
Non-Ground Food Blender 2 mm 6 mm 10 mm
%conc.
Cannabinoid Concentration F1
Lower is better, 10mm wins
55
60
65
70
75
Non-Ground Food Blender 2 mm 6 mm 10 mm
%conc.
Cannabinoid Concentration F2
Higher is better, 2mm wins
Extraction Precision Cannabinoids
• 3 kg of plant matter
• Terpene Fraction (F1): 1100 psi, 34˚C, 5 h
• Cannabinoid Fraction (F2): 1900 psi, 60˚C, 12 h
19. Extraction
• Particle size effects yield and precision
• SFE can extract terpene fractions
• SFE can extract either THCA or THC
2mm
Whole
Flower
Food
Blender
Terpenes
THC
THCA
Milling Method
Extraction Method
20. Extraction
• Particle size effects yield and precision
• SFE can extract terpene fractions
• SFE can extract either THCA or THC
• SFE can change the THC to CBD ratio
2mm
Milling Method
Extraction Method
Terpenes
THC
THCA
CBDA
Whole
Flower
Food
Blender
21. Analyzing CO2 SFE for THC Recovery (Super)
• a= 14.9 xT + 123 xp + 18.4 xTxp
22. Analyzing CO2 SFE for THC Recovery (Sub)
• a= - 7.75 xT + 126 xp + 59.3 xTxp
23. Analyzing CO2 SFE for %Terpene (Super)
• a= - 14.9 xT - 11.7 xp + 16.8 xTxp
24. Analyzing CO2 SFE for %Terpene (Sub)
• a= 11.8 xT - 16.6 xp - 12.5 xTxp
25. Comparing Sub- and Supercritical
• SFE, 900.0 – 1900 psi, 5.0 – 60 ˚C, 22h max
Response Sub Super
THC Recovery 60% 80%
Terpene Fraction 52% at 34% yield 63% at 82% yield
26. Lessons learned in SFE
• THC extracts faster than THCA
• THC to THCA ratio adjusted by SFE
% Decarboxylation S.M. 7h 22h
Exp. B 8.0% 25% 10%
Exp. J 14% 25% 14%
27. Lessons learned in SFE
• CBD extracts faster than THC
• CBD to THC ratio adjusted by SFE
CBD/THC S.M. 7h 22h
Exp. A 4.1E-03 1.3E-02 5.0E-03
Exp. J 1.7E-04 1.3E-03 3.1E-03
Bonus: Decarboxylation of CBDA
slower than for THCA
J. of Supercritical Fluids, 2010, 603
28. Lessons learned in SFE
• Enrichment in minor cannabinoids
• CBG enrichment by SFE
CBG/total Canna. S.M. 7h 22h
Exp. B 2.9% 6.% 2.9%
Exp. J 3.0% 13% 4.9%
J. of Supercritical Fluids, 2010, 603
29. Lessons learned in SFE
• Changing terpene profiles in SFE
• Sativa: rich in monoterpenes
• Indica: rich in sesquiterpenes
Hazekamp, et al.; Cannabis and Cannabinoid Research 2016, 202