Primary cells are cells directly isolated from living differentiated tissues (animal or plant) that retain the physiological characteristics of the tissue of origin. Unlike cell lines, they have finite lifespan and are true representative of the genetic make up of organism.
this presentation depicts the usefulness of single cell profiling in crop plant for identifying novel gene sources which can be used for crop improvement
A statistical framework for multiparameter analysis at the single cell levelShashaanka Ashili
Phenotypic characterization of individual cells provides crucial insights into intercellular heterogeneity and enables access to information that is unavailable from ensemble averaged, bulk cell analyses. Single-cell studies have attracted significant interest in recent years and spurred the development of a variety of commercially available and research-grade technologies. To quantify cell-to-cell variability of cell populations, we have developed an experimental platform for real-time measurements of oxygen consumption (OC) kinetics at the single-cell level. Unique challenges inherent to these single-cell measurements arise, and no existing data analysis
methodology is available to address them. Here we present a data processing and analysis method that addresses challenges encountered with this unique type of data in order to extract biologically relevant information. We applied the method to analyze OC profiles obtained with single cells of two different cell lines derived from metaplastic and dysplastic human Barrett’s esophageal epithelium. In terms of method development, three main challenges were considered for this heterogeneous dynamic system: (i) high levels of noise, (ii) the lack of a priori knowledge of single-cell dynamics, and (iii) the role of intercellular variability within and across cell types.
Several strategies and solutions to address each of these three challenges are presented. The features such as slopes, intercepts, breakpoint or change-point were extracted for every OC profile and compared across individual cells and cell types. The results demonstrated that the extracted features facilitated exposition of subtle differences between individual cells and their responses to
cell–cell interactions. With minor modifications, this method can be used to process and analyze
data from other acquisition and experimental modalities at the single-cell level, providing a valuable statistical framework for single-cell analysis.
The cell and its evolution:
*Stanford researchers produce 12 cell types from human stem cells in days.
*Study uncovers new drug-gene mutation combinations that can kill cancer cells
this presentation depicts the usefulness of single cell profiling in crop plant for identifying novel gene sources which can be used for crop improvement
A statistical framework for multiparameter analysis at the single cell levelShashaanka Ashili
Phenotypic characterization of individual cells provides crucial insights into intercellular heterogeneity and enables access to information that is unavailable from ensemble averaged, bulk cell analyses. Single-cell studies have attracted significant interest in recent years and spurred the development of a variety of commercially available and research-grade technologies. To quantify cell-to-cell variability of cell populations, we have developed an experimental platform for real-time measurements of oxygen consumption (OC) kinetics at the single-cell level. Unique challenges inherent to these single-cell measurements arise, and no existing data analysis
methodology is available to address them. Here we present a data processing and analysis method that addresses challenges encountered with this unique type of data in order to extract biologically relevant information. We applied the method to analyze OC profiles obtained with single cells of two different cell lines derived from metaplastic and dysplastic human Barrett’s esophageal epithelium. In terms of method development, three main challenges were considered for this heterogeneous dynamic system: (i) high levels of noise, (ii) the lack of a priori knowledge of single-cell dynamics, and (iii) the role of intercellular variability within and across cell types.
Several strategies and solutions to address each of these three challenges are presented. The features such as slopes, intercepts, breakpoint or change-point were extracted for every OC profile and compared across individual cells and cell types. The results demonstrated that the extracted features facilitated exposition of subtle differences between individual cells and their responses to
cell–cell interactions. With minor modifications, this method can be used to process and analyze
data from other acquisition and experimental modalities at the single-cell level, providing a valuable statistical framework for single-cell analysis.
The cell and its evolution:
*Stanford researchers produce 12 cell types from human stem cells in days.
*Study uncovers new drug-gene mutation combinations that can kill cancer cells
Creative Bioarray offers 35 human cell systems with over 160 different cell types. Moreover, we also provide our customers with primary cells from over 13 types of other animals.
https://www.creative-bioarray.com/products/primary-cell-types-list-14.htm?page=1
Austin Cell Biology strongly supports the scientific up gradation and fortification in related scientific research community by enhancing access to peer reviewed scientific literary works.
Lecture presented by Dr.Fatma Taha at BIOCHEM Cairo 2014 organized by Department of Medical Biochemistry and Molecular Biology, Cairo University. BIOCHEM Cairo 2014 is a Scribe event ( www.scribeofegypt.com)
EXTRACTION AND CLASSIFICATION OF BLEBS IN HUMAN EMBRYONIC STEM CELLdbpublications
A main objective of this paper is
to extract bleb from the human
embryonic stem cells. Blebbing is an
important biological indicator in
determining the health of human
embryonic stem cells (hESC). Especially,
areas of a bleb sequence in a video are
often used to distinguish two cells
blebbing behaviours in HESC; dynamic
and apoptotic blessings. Here analyses
active contour segmentation method for
bleb extraction in hESC videos and
introduces a bio-inspired score function
to improve the performance in bleb
extraction. The full bleb formation
consists of bulb expansion and retraction.
Blebs change their size and image
properties dynamically in both processes
and between frames. Therefore, adaptive
parameters are needed for each
segmentation method. A score function
derived from the change of bleb area and
orientation between consecutive frames with cuckoo optimization is proposed
which provides adaptive parameters for
bleb extraction in videos and classified
using artificial neural networks (ANN).
evidences of anatomy, cytology and chemistry to plant taxonomynasira jaffry
taxonomy is based on other disciplines of sciences. in this presentation, there is discussion how anatomy, cytology and chemistry influnces the taxonomy
Exosomes are smallest extracellular vesicles of size 30 to 100 nm originated from late endosomes. These are released by broad array of cells including B‐ cells, cells, dendritic cells (DCs), T‐cells, epithelial cells,
platelets and many more.
Creative Bioarray offers 35 human cell systems with over 160 different cell types. Moreover, we also provide our customers with primary cells from over 13 types of other animals.
https://www.creative-bioarray.com/products/primary-cell-types-list-14.htm?page=1
Austin Cell Biology strongly supports the scientific up gradation and fortification in related scientific research community by enhancing access to peer reviewed scientific literary works.
Lecture presented by Dr.Fatma Taha at BIOCHEM Cairo 2014 organized by Department of Medical Biochemistry and Molecular Biology, Cairo University. BIOCHEM Cairo 2014 is a Scribe event ( www.scribeofegypt.com)
EXTRACTION AND CLASSIFICATION OF BLEBS IN HUMAN EMBRYONIC STEM CELLdbpublications
A main objective of this paper is
to extract bleb from the human
embryonic stem cells. Blebbing is an
important biological indicator in
determining the health of human
embryonic stem cells (hESC). Especially,
areas of a bleb sequence in a video are
often used to distinguish two cells
blebbing behaviours in HESC; dynamic
and apoptotic blessings. Here analyses
active contour segmentation method for
bleb extraction in hESC videos and
introduces a bio-inspired score function
to improve the performance in bleb
extraction. The full bleb formation
consists of bulb expansion and retraction.
Blebs change their size and image
properties dynamically in both processes
and between frames. Therefore, adaptive
parameters are needed for each
segmentation method. A score function
derived from the change of bleb area and
orientation between consecutive frames with cuckoo optimization is proposed
which provides adaptive parameters for
bleb extraction in videos and classified
using artificial neural networks (ANN).
evidences of anatomy, cytology and chemistry to plant taxonomynasira jaffry
taxonomy is based on other disciplines of sciences. in this presentation, there is discussion how anatomy, cytology and chemistry influnces the taxonomy
Exosomes are smallest extracellular vesicles of size 30 to 100 nm originated from late endosomes. These are released by broad array of cells including B‐ cells, cells, dendritic cells (DCs), T‐cells, epithelial cells,
platelets and many more.
For decades, cell lines have played a critical role in scientific developments. In most cases, researchers just got data generated from cell lines. However, due to some weaknesses of cell lines, scientists become increasingly cautious about these generated results. But now the game has changed! Primary cells now are believed to be a more biologically relevant tool than cell lines for studying human and animal biology. And we design this primary cell culture guide aimed at showing new investigators the basic principles of primary cell and some practical culture skills.
In conclusion, both cell strains and primary cells have played vital roles in advancing our understanding of cellular processes, disease mechanisms, and drug discovery. While cell strains provide a continuous and convenient supply of cells, primary cells offer a more authentic representation of human biology, enabling researchers to study specific tissues and diseases in a physiologically relevant context. The growth of cell strains and the significance of primary cells have propelled biomedical research forward, leading to breakthroughs in various fields and fostering the development of innovative therapeutic strategies. As technologies continue to evolve, primary cells will remain indispensable in further unraveling the complexities of human health and disease.
introduction to cell biology
Cell Biology
The cell is a chemical system that can maintain its structure and reproduce.
Cells are the fundamental unit of life.
All living things are cells or composed of cells.
The interior contents of cells in the cytoplasm
The cell is a chemical system that can maintain its structure and reproduce.
Cells are the fundamental unit of life.
All living things are cells or composed of cells.
The interior contents of cells in the cytoplasm.
Cell, in biology, the basic membrane-bound unit that contains the fundamental molecules of life and of which all living things are composed.
A single cell is often a complete organism in itself, such as a bacterium or yeast.
Other cells acquire specialized functions as they mature.
These cells cooperate with other specialized cells and become the building blocks of large multicellular organisms, such as humans and other animals.
The smallest known cells are a group of tiny bacteria called mycoplasmas; some of these single-celled organisms are spheres as small as 0.2 μm in diameter.
Largest cell ….
The largest single-celled organism is an animal
called Syringammina fragilissima, which can grow to a width of 4 inches.
– are highly folded, forming a complex network of tubes.
The biological science which deals with the study of structure, function, molecular organization, growth, reproduction, and genetics of the cells, is called cytology or cell biology
(Gr., kytos = hollow vessel or cell; logos = to discourse).
Much of cell biology is devoted to the study of structures and functions of specialized cells.
Individual cells that form our bodies can grow, reproduce, process information, respond to stimuli, and carry out an amazing array of chemical reactions.
SYNTHETIC CELLS
An artificial cell or minimal cell or synthetic cell is an engineered particle that mimics one or many functions of a biological cell.
Artificial cells are biological or polymeric membranes which enclose biologically active materials.
A "living" artificial cell has been defined as a completely synthetically made cell that can capture energy, maintain ion gradients, contain macromolecules as well as store information and have the ability to mutate.
DEFINITION
EXAMPLE
SYNTHETIC BIOLOGY
Synthetic biology is a multidisciplinary area of research that seeks to create new biological parts, devices, and systems, or to redesign systems that are already found in nature.
Due to more powerful genetic engineering capabilities and decreased DNA synthesis and sequencing costs, the field of synthetic biology is rapidly growing
HISTORY
BOTTOM-UP APPROACH FOR CONSTRUCTING SYNTHETIC CELLS
A bottom-up approach is commonly used to design and construct genetic circuits by piecing together functional modules that are capable of reprogramming cells with novel behavior.
CELL ENCAPSULATION METHOD
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins.
TECHNIQUES USED FOR THE PREPARATION OF EMULSION
1- high pressure homogenization
2- microfluidization
3- drop method
4- emulsion method
MEMBRANES OF SYNTHETIC CELLS
THE MINIMAL CELL
A minimal cell is one whose genome only encodes the minimal set of genes necessary for the cell to survive.
THE SYNTHETIC BLOOD CELLS
Synthetic red blood cells mimic natural ones, and have new abilities
APPLICATIONS OF SYNTHETIC CELLS
1- DRUG RELEASE AND DELIEVERY
2- GENE THERAPY
3- ENZYME THERAPY
4- HEMOPERFUSION
5- OTHER APPLICATIONS
FUTURE OF SYNTHETIC CELLS AND BIOLOGY
ACHIEVEMENTS
HEALTH AND SAFETY ISSUES
ETHICS AND CONTROVERSIES
REFERENCES
THANK YOU
Cell Biology Understanding the Building of Life.pptxSSSI .
The study of the composition, operation, and behavior of cells—the essential building blocks of life is the subject of cell biology, a fundamental discipline of science
Similar to KOSHEEKA PRIMARY CELLS : PHYSIOLOGICAL MODELS OF RELEVANCE (20)
The pursuit of understanding cellular processes and their intricate interplay with external stimuli lies at the heart of modern biomedical research. In this context, assay development in cell culture has emerged as an indispensable tool, allowing scientists to investigate cellular responses, signalling pathways, drug effects, and disease mechanisms in a controlled and replicable environment. This essay delves into the significance of assay development in cell culture, its methodologies, applications, and contributions to advancing scientific knowledge.
Cell culture is an essential technique used in many fields, including biotechnology, pharmaceuticals, and medical research. It involves the growth and maintenance of cells in a controlled environment, typically in a laboratory setting. Let’s explore some of the challenges and solution associated with scaling up cell culture.
Maintaining Cell Viability
Contamination
Optimization of Culture Medium
Scale-up Cost
Website:- https://kosheeka.com/
Mesenchymal stem cells (MSCs) are a type of adult stem cell that can differentiate into a variety of cell types, including osteoblasts (bone cells), chondrocytes (cartilage cells), myocytes (muscle cells), and adipocytes (fat cells). MSCs have the potential to be used for regenerative medicine, tissue engineering, and cell therapy due to their ability to self-renew and differentiate. Several types of MSCs can be categorized based on their origin and potential for differentiation.
1: Bone Marrow-Derived MSCs
2: Adipose Tissue-Derived MSCs
3: Umbilical Cord-Derived MSCs
4: Dental Pulp-Derived MSCs
5: Synovial-Derived MSCs
Primary human cells are cells that have been taken out of human tissues like bone marrow and blood. They are recognised as a crucial tool for developing in vivo models, such as xenograft or humanised mice, or for in vitro cell-based tests.
Cancer stem cells (CSC) are currently one of the most complex targets for cancer therapy. Cancer hierarchy has developed as a revolutionary framework over the last two decades, altering our understanding of tumour heterogeneity and cancer therapeutic administration.
KOSHEEKA is powered by Advancells: India’s premier regenerative medicine solution provider. We provide authentic, high quality, standard primary cells and stem cells required for scientific animal cell culture research and breakthrough in the field of healthcare.
Kosheeka is the premier provider of regenerative medicine solutions in India. The company
promotes the production of customized primary cells and stem cells essential for
groundbreaking scientific advancements. The company is a pioneer in stem cell research and
has established massive production of Mesenchymal Stem Cells (MSCs).
In vitro models are crucial tools in cancer research because they assist researchers to identify carcinogens, developing cancer therapies, drug discovery, and study about the molecular pathways of tumour growth and spread. Cancer cells are an essential part of any in vitro tumour model. Cancer cell lines are simple to culture, allow for quick comparisons of experiments, and are commonly employed to research tumour cell biology molecular pathways. Tumour cell biology, 3D cell culture, tissue engineering, biomaterials, microfabrication, and microfluidics advancements have facilitated the dynamic development of in vitro tumour models.
In vitro models are crucial tools in cancer research because they assist researchers to identify carcinogens, developing cancer therapies, drug discovery, and study about the molecular pathways of tumour growth and spread. Cancer cells are an essential part of any in vitro tumour model.
Cell culture is a realm within itself. The medium in the culture dish includes nutrients that cells require to live and thrive. But, every once in a while, uninvited invaders breach the cultures, endangering the delicate stability of the system and tainting the scientific conclusions.
KOSHEEKA is powered by Advancells: India’s premier regenerative medicine solution provider. Our cell culture processing and storage unit is ISO 9001:2015 certified and GLP accredited.
More from Kosheeka : Primary Cells for Research (11)
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
New Drug Discovery and Development .....NEHA GUPTA
The "New Drug Discovery and Development" process involves the identification, design, testing, and manufacturing of novel pharmaceutical compounds with the aim of introducing new and improved treatments for various medical conditions. This comprehensive endeavor encompasses various stages, including target identification, preclinical studies, clinical trials, regulatory approval, and post-market surveillance. It involves multidisciplinary collaboration among scientists, researchers, clinicians, regulatory experts, and pharmaceutical companies to bring innovative therapies to market and address unmet medical needs.
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
2. WHAT ARE PRIMARY CELLS ?
Primary cells are cells directly isolated from living differentiated tiss
ues (animal or plant) that retain the physiological characteristics of t
he tissue of origin. Unlike cell lines, they have finite lifespan and ar
e true representative of the genetic make up of organism.
ADHERENT
CULTURE
SUSPENSION
CULTURE
4. PRIMARY CELL APPLICATIONS
Maximum permissible
drug dosage
1. Drug screening &
Toxicity analysis
Reduction of cost, time
and ethical issues
3. Substitute for
animal studies
Resemblance to
in-vivo environment
2. Cell Biology &
metabolic research
Reconstruction of
damaged organs
4. Tissue
engineering
1.
2.
3.
4.
5. WHY PRIMARY CELLS OVER CELL
LINES?
CLOSELY MIMIC IN VIVO
ENVIRONMENT.
MINIMAL CHROMOSOMAL
ABERRATIONS.
RETAIN FUNCTIONALITY.
Primary cells are true models of physiological relevance that represent
reliable data
6. FUTURE SCOPE
Primary cells are now being explored for research in 3D systems for translational applications and
better study models
3D Organoid 3D Bioprinting Biochips
7. Why KOSHEEKA ?
Our cell processing and storage unit is ISO 9001:2015 certified and GLP accredited
PURE
POPULATION
VERY EARLY
PASSAGE
HIGH QUALITY
&RELIABILITY
ON-DEMAND
ISOLATION
8. QUALITY CONTROL
Kosheeka primary cells are subjected to
strict quality control and authentication
Testing for mycoplasma, bacteria
and fungi
Authenticated
Highly characterized
Processing under BSL 3 category
Specialized media for every cell
9. SHIPPING
• On User Demand-Live cells and frozen
• High cell viability
• Cell count and authentication certificate
• Media details and instructions in CoA