The document summarizes a study that evaluated the antihyperglycemic and antinociceptive activities of the methanolic extract of Kaempferia rotunda rhizomes. In glucose tolerance tests in mice, the extract significantly lowered serum glucose levels in a dose-dependent manner, with the highest dose of 400 mg/kg reducing glucose by 39.6%. In antinociceptive tests using acetic acid-induced writhing in mice, the extract significantly reduced the number of writhes in a dose-dependent manner, with the highest dose of 400 mg/kg reducing writhes by 69.4%. The extract demonstrated significant antihyperglycemic and antinociceptive effects,
In vitro antidiabetic activity of Clerodendrum viscosum VentSriramNagarajan17
The study evaluated the antidiabetic effects of Clerodendrum viscosum, a common plant in Bangladesh, on alloxan-induced diabetic mice. Mice were divided into five groups: a normal control group, an alloxan-induced diabetic control group, two groups treated with 250 mg/kg or 500 mg/kg of the C. viscosum extract, and a group treated with the antidiabetic drug glibenclamide. The extract significantly reduced blood glucose levels in a dose-dependent manner, with the 500 mg/kg dose showing stronger effects compared to the 250 mg/kg dose. The extract was found to have antidiabetic properties and lower blood glucose in diabetic mice.
1) The document describes a study that evaluated the hepatoprotective potential of extracts from in vitro regenerated Boerhaavia diffusa plants compared to extracts from naturally grown plants.
2) Mice of both sexes were intoxicated with carbon tetrachloride to induce hepatic injury, then treated with extracts from in vitro plants or natural plants. Serum levels of liver enzymes (SGOT and SGPT) were measured to assess hepatoprotection.
3) Treatment with both in vitro and natural plant extracts significantly reduced elevated liver enzyme levels compared to untreated intoxicated mice, indicating both were effective at protecting the liver from carbon tetrachloride toxicity. This suggests the in vitro regenerated plants produced bioactive molecules with
Analgesic and Anti-diarrheal Activities of Aganosma dichotoma (Roth)Aranno Hossain
Analgesic and Anti-diarrheal Activities of Aganosma dichotoma (Roth)
K. Schum. in Swiss-Albino Mice Model
Md. Al Faruk1, Mohammad Firoz Khan2, Md. Yeunus Mian2, Mohammad Sharifur Rahman3
and Mohammad A. Rashid3
This document describes a study that evaluated the anti-diabetic effects of an Ayurvedic herbo-mineral formulation called Trushanadi loha. The formulation contains 14 plant and mineral ingredients. An aqueous extract was tested in rats. Blood glucose levels were measured. The extract produced a 40% reduction in blood glucose levels in normal rats, demonstrating significant anti-diabetic activity. The formulation was also found to be non-toxic in acute oral toxicity tests in rats at doses up to 5,000 mg/kg. Overall, the study provides evidence that Trushanadi loha has anti-diabetic properties and a safe toxicity profile.
Antihyperglycemic Effect of Aqueous Leaf Extract of Mimusops elengi against S...BRNSS Publication Hub
Objective: The present study was hypothesized to evaluate the antihyperglycemic effect of aqueous leaf extract of Mimusops elengi on streptozotocin (STZ)-induced diabetic animals. Materials and Methods: Antidiabetic activity of M. elengi leaf extract at a dosage of 250 mg/kg body weight was evaluated. Results: The activity levels of glucose, triglycerides, total cholesterol, and serum glutamic pyruvic transaminase were significantly elevated in STZ-induced diabetic animals when compared to that of normal animals. After supplemented with aqueous leaf extract of M. elengi, animals group recorded significant lower blood glucose level. Conclusion: The aqueous leaf extract of M. elengi has been potent antidiabetic effect in male albino rat.
Hepatoprotective Effect of Cestrum parqui L. aerial parts and Phytochemical ...Jing Zang
This study deals with the investigation of hepatoprotective effect of 70% methanolic extract from Cestrum parqui aerial parts and determination of the bioactive components of the plant. The hepatoprotective effect of Cestrum parqui methanol extract (100, 500, 1000 mg/kg) was analysed on carbon tetrachloride (CCl4)-induced acute liver injury. The administration of a single dose of 40% CCl4 (1ml/kg b.w.) causes an increase in the activities of serum alanine aminotransferase (ALT) and aspirate aminotransferase (AST) enzymes and so pretreated orally of a dose from Cestrum parqui methanol extract (100, 500, 1000 mg/kg) and silymarin (200 mg/kg) for three consecutive days prior to The administration of a single dose of CCl4 significantly prevented the increase in the activities of these enzymes. Histological analysis showed that Cestrum parqui methanol extract at doses of 500 and 1000 mg/kg and silymarin reduced the incidence of liver lesions including vacuole formation, neutrophil infiltration and necrosis of hepatocytes induced by CCl4. The extract cause a negative result on the antioxidative enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GRd) and decreased malondialdehyde (MDA) level in liver, as compared to those in the CCl4-treated group and this suggests that the hepatoprotective activity of the extract is due to the antioxidant effect of the extract. Phytochemical analysis of the methanol extract from Cestrum parqui aerial parts showed that it contained different phytoconstituents, flavonoids, tannins, saponins, alkaloids, terpenes and carbohydrates.
This study evaluated the antiasthmatic potential of a polyherbal formulation containing extracts of four plants - Solanum xanthocarpum, Aegle marmelos, Caesalpinia bonduc, and Murraya koenigii. Experimental models in rats and guinea pigs were used to assess mast cell stabilization, antihistaminic, and anticholinergic effects. The results demonstrated that the polyherbal formulation dose-dependently inhibited mast cell degranulation and protected against histamine- and acetylcholine-induced bronchospasm in guinea pigs, suggesting antiasthmatic effects through mast cell membrane stabilization, antihistaminic, and anticholinergic mechanisms of action. This
In vitro antidiabetic activity of Clerodendrum viscosum VentSriramNagarajan17
The study evaluated the antidiabetic effects of Clerodendrum viscosum, a common plant in Bangladesh, on alloxan-induced diabetic mice. Mice were divided into five groups: a normal control group, an alloxan-induced diabetic control group, two groups treated with 250 mg/kg or 500 mg/kg of the C. viscosum extract, and a group treated with the antidiabetic drug glibenclamide. The extract significantly reduced blood glucose levels in a dose-dependent manner, with the 500 mg/kg dose showing stronger effects compared to the 250 mg/kg dose. The extract was found to have antidiabetic properties and lower blood glucose in diabetic mice.
1) The document describes a study that evaluated the hepatoprotective potential of extracts from in vitro regenerated Boerhaavia diffusa plants compared to extracts from naturally grown plants.
2) Mice of both sexes were intoxicated with carbon tetrachloride to induce hepatic injury, then treated with extracts from in vitro plants or natural plants. Serum levels of liver enzymes (SGOT and SGPT) were measured to assess hepatoprotection.
3) Treatment with both in vitro and natural plant extracts significantly reduced elevated liver enzyme levels compared to untreated intoxicated mice, indicating both were effective at protecting the liver from carbon tetrachloride toxicity. This suggests the in vitro regenerated plants produced bioactive molecules with
Analgesic and Anti-diarrheal Activities of Aganosma dichotoma (Roth)Aranno Hossain
Analgesic and Anti-diarrheal Activities of Aganosma dichotoma (Roth)
K. Schum. in Swiss-Albino Mice Model
Md. Al Faruk1, Mohammad Firoz Khan2, Md. Yeunus Mian2, Mohammad Sharifur Rahman3
and Mohammad A. Rashid3
This document describes a study that evaluated the anti-diabetic effects of an Ayurvedic herbo-mineral formulation called Trushanadi loha. The formulation contains 14 plant and mineral ingredients. An aqueous extract was tested in rats. Blood glucose levels were measured. The extract produced a 40% reduction in blood glucose levels in normal rats, demonstrating significant anti-diabetic activity. The formulation was also found to be non-toxic in acute oral toxicity tests in rats at doses up to 5,000 mg/kg. Overall, the study provides evidence that Trushanadi loha has anti-diabetic properties and a safe toxicity profile.
Antihyperglycemic Effect of Aqueous Leaf Extract of Mimusops elengi against S...BRNSS Publication Hub
Objective: The present study was hypothesized to evaluate the antihyperglycemic effect of aqueous leaf extract of Mimusops elengi on streptozotocin (STZ)-induced diabetic animals. Materials and Methods: Antidiabetic activity of M. elengi leaf extract at a dosage of 250 mg/kg body weight was evaluated. Results: The activity levels of glucose, triglycerides, total cholesterol, and serum glutamic pyruvic transaminase were significantly elevated in STZ-induced diabetic animals when compared to that of normal animals. After supplemented with aqueous leaf extract of M. elengi, animals group recorded significant lower blood glucose level. Conclusion: The aqueous leaf extract of M. elengi has been potent antidiabetic effect in male albino rat.
Hepatoprotective Effect of Cestrum parqui L. aerial parts and Phytochemical ...Jing Zang
This study deals with the investigation of hepatoprotective effect of 70% methanolic extract from Cestrum parqui aerial parts and determination of the bioactive components of the plant. The hepatoprotective effect of Cestrum parqui methanol extract (100, 500, 1000 mg/kg) was analysed on carbon tetrachloride (CCl4)-induced acute liver injury. The administration of a single dose of 40% CCl4 (1ml/kg b.w.) causes an increase in the activities of serum alanine aminotransferase (ALT) and aspirate aminotransferase (AST) enzymes and so pretreated orally of a dose from Cestrum parqui methanol extract (100, 500, 1000 mg/kg) and silymarin (200 mg/kg) for three consecutive days prior to The administration of a single dose of CCl4 significantly prevented the increase in the activities of these enzymes. Histological analysis showed that Cestrum parqui methanol extract at doses of 500 and 1000 mg/kg and silymarin reduced the incidence of liver lesions including vacuole formation, neutrophil infiltration and necrosis of hepatocytes induced by CCl4. The extract cause a negative result on the antioxidative enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GRd) and decreased malondialdehyde (MDA) level in liver, as compared to those in the CCl4-treated group and this suggests that the hepatoprotective activity of the extract is due to the antioxidant effect of the extract. Phytochemical analysis of the methanol extract from Cestrum parqui aerial parts showed that it contained different phytoconstituents, flavonoids, tannins, saponins, alkaloids, terpenes and carbohydrates.
This study evaluated the antiasthmatic potential of a polyherbal formulation containing extracts of four plants - Solanum xanthocarpum, Aegle marmelos, Caesalpinia bonduc, and Murraya koenigii. Experimental models in rats and guinea pigs were used to assess mast cell stabilization, antihistaminic, and anticholinergic effects. The results demonstrated that the polyherbal formulation dose-dependently inhibited mast cell degranulation and protected against histamine- and acetylcholine-induced bronchospasm in guinea pigs, suggesting antiasthmatic effects through mast cell membrane stabilization, antihistaminic, and anticholinergic mechanisms of action. This
Effects of Metformin, Pioglitazone and Aqueous Extract of Delonix Regia on Bl...iosrjce
The effects of Delonix regia extract (d200mg, d300mg, and d400mg), metformin (m8.3mg, m12.5mg
and m16.5mg), pioglitazone (p0.5mg, p0.7mg and p0.9mg) and combined formulation of metformin and extract
(m6.25d150mg) on glycated hemoglobin status in streptozotocin-induced diabetic Albino wistar rats. Diabetic
status of these rats was assessed by estimating fasting blood glucose levels. A total of 150 albino rats were used
for the investigation and were grouped into twelve groups of twelve rats each as follows; Group I: normal
control rats (NCR). Group II: Diabetic control rats (DCR). Group III: Diabetic rats treated with d200mg.
Group IV: Diabetic rats treated with d300mg. Group V: Diabetic rats treated with d400mg. Group VI: Diabetic
rats treated with m8.3mg. Group VII: Diabetic rats treated with m12.5mg. Group VIII: Diabetic rats treated
with m16.5mg. Group IX: Diabetic rats treated with p0.5mg. Group X: Diabetic rats treated with p0.75mg.
Group XI: Diabetic rats treated with p1.0mg. Group XII: Diabetic rats treated with m125d300mg each for male
and female respectively, for a total of 56 days. After every two weeks interval of treatment for eight weeks three
rats from each group were sacrificed and blood sample were collected and analyzed for various parameters.
The result obtained showed an elevated level of glycated hemoglobin in diabetic-induced wistar albino rats
compared with normal control rats. However, there was reversal of the effects when treated with the
drug/extract. Also there was reduction in the blood glucose level of the diabetic rats treated with metformin
(from 6.37±0.69 to 5.20±0.62mmol/l), pioglitazone (from 7.30±0.21mmol/l to 4.70±0.46), aqueous extract of
Delonixregia (from 8.20±0.81mmol/l to 6.10±0.60) and combined formulation of metformin and extract (from
7.81±0.34 to 4.80±0.17), at p<0.05 confidence level when compared with diabetic control rats in the various
weeks of treatment respectively
This research article studied the anti-obesity effects of Moringa oleifera leaf extract (MEMOL) in rats with high fat diet-induced obesity. Rats were fed a high fat diet to induce obesity over 49 days. Treatment with MEMOL for 49 days significantly reduced body weight, total cholesterol, triglycerides, and LDL levels in obese rats, and increased body temperature, compared to untreated obese rats. MEMOL treated rats also showed decreased levels of liver enzymes and blood glucose. The results indicate that MEMOL attenuated body weight gain in obese rats without affecting food intake, and demonstrated hypoglycemic and hypolipidemic effects, suggesting it may help treat obesity and related disorders.
This research article evaluated the potential aphrodisiac effects of Moringa oleifera seed extracts in male albino rats. Researchers administered aqueous, alcohol, and chloroform extracts of M. oleifera seeds to male rats for 21 days. They observed several parameters of sexual behavior and found that the extracts significantly increased mounting frequency, intromission frequency, and ejaculation latency, while decreasing mounting latency, intromission latency, and post-ejaculatory interval. The extracts also increased libido without any adverse effects. The results suggest that M. oleifera seed extracts can enhance sexual behavior in male rats, supporting its traditional use as an aphrodisiac.
The document evaluates the analgesic and anti-inflammatory activities of Karuvilanchi ver chooranam (KVC), the root powder of Smilax zeylanica, in rodent models. The results showed that KVC significantly increased the reaction time in hot plate test and inhibited acetic acid-induced writhing responses in mice, indicating its analgesic effects. KVC also significantly reduced formalin-induced paw edema in rats and cotton pellet-induced granuloma formation, demonstrating its anti-inflammatory properties. The study confirms the traditional use of KVC for pain and inflammation management.
The document summarizes a study that analyzed samples collected from diarrhea patients and their mothers in rural Bangladesh to detect coliform bacteria. Several species of coliform bacteria were isolated from the samples, with isolation rates ranging from 38.01-3.51%. Extracts of Moringa oleifera leaves were tested against the isolated bacteria. The leaf extracts showed antibacterial effects and were able to inhibit the growth of all tested bacterial pathogens, including E. coli, Shigella, Salmonella, Enterobacter, Klebsiella, and Serratia species. The extracts had zone of inhibition ranging from 8-23 mm and minimum inhibitory concentration values between 62.5-1000 μg/mL, suggesting M. oleifera extracts could
Background: The probable mechanism of an earlier reported capacity of palm oil extracts to confer protection
against high dose cadmium poisoning in rats was reported in this study. Similar experimental design earlier reported
by us was retained. Rats therefore were sacrificed at intervals of twelve; twenty four and forty eight hours post CdCl2
insult.
Results: Oxidative stress and antioxidant status (malondialdehyde, superoxide dismutase, catalase and glutathione)
were assessed in tissues (liver, kidney, heart, brain, muscle) and serum. Oxidative stress indicators showed a significantly
(p < 0.05) increased lipid peroxidation and alterations in antioxidant defence systems occasioned by drop in
catalase and superoxide dismutase enzymes (serum, liver, heart, brain and kidneys) of the rats. Also observed were
significant (p < 0.05) reduction in the non-enzymatic antioxidant reduced glutathione over time. Pre-administration of
rats with the crude palm oil and its extracts modulated cadmium mediated depletion of the antioxidant capacities of
rats acutely exposed to cadmium and rising lipid peroxidation profile.
Conclusions: Regulation of stress and antioxidant response was the underlying mechanism by which the extracts
conferred protection against high dose cadmium insult thus suggesting its potential as a viable therapeutic target
against its deleterious effects.
The use of medicinal plants in the treatment of harmful impacts of xenobiotics in animals is attracting an increasing attention in recent times. The aim of the current study is to assess the preventive potential of Costus afer aqueous leaves extract (CAAE) in treating metabolic aberrations imposed by crude oil contaminated diet in Wistar albino rats. Six groups of rats were treated as follows: A = Normal diet; B= Normal diet + 100 mg/kg body weight of CAAE; C =Normal diet + 200 mg/kg body weight of CAAE; D= Crude oil contaminated diet; E= crude oil contaminated diet + 100 mg/kg body weight of CAAE, F = crude oil contaminated diet + 200 mg/kg body weight of CAAE. After thirty days of exposure to the diet and administration of the corresponding plant extracts, the rats were sacrificed with chloroform and the required organs were excised. The hematological indices, as well as function indicators and levels of drug metabolizing enzymes in the liver and kidney, were investigated with standard protocols. The results indicated that the hematological parameters and kidney and liver function indices were altered in rats fed with crude oil contaminated diet. However, the values came close to those in control rats when Costus afer aqueous extracts were administered. Similarly, the activities of oxidase enzymes (aldehyde oxidase, monoamine oxidase, xanthine oxidase, and sulphite oxidase), following their inhibition by the ingestion of crude oil contaminated diet, equally restored close to control values upon treatment with Costus afer aqueous extract. This study, therefore, was able to establish an aqueous extract of Costus afer leave as an antidote for crude oil intoxication.
Blood glucose and lipid reducing activities of the oral administration of aqu...Alexander Decker
1. The study investigated the effects of repeated oral administration of an aqueous extract of Moringa oleifera leaves on blood glucose and lipid levels in male Wistar rats over 56 days.
2. The extract significantly reduced blood glucose and serum lipid levels like total cholesterol and triglycerides in a dose-dependent manner. It also significantly increased HDL cholesterol.
3. The extract maintained or slightly reduced body weight in the rats. It significantly reduced food intake, which may be due to effects on appetite or metabolism in the gastrointestinal tract.
Synthesis, Characterization, and Biological Evaluation of Some Novel Phthalim...BRNSS Publication Hub
Objective: Different Phthalimide derivatives (4,5,6,7-tetrachloro-2-[1,2,4]triazol-4-yl]-isoindole-1,3-dione derivatives) were synthesized and biological activities of them were evaluated. Materials and Methods: In the present study, four new phthalimide derivatives were synthesized. The structures of final compounds were characterized on the basis of spectral data. Then, biological evaluation of all the synthesized compounds means in vivo anticancer activity was evaluated on the Ehrlich ascites carcinoma (EAC) bearing Swiss albino mice model, and in vitro antioxidant activity was assessed using 1,1-diphenyl-2-picryl hydrazine (DPPH) radical scavenging assay. Results: The titled compounds (2A-2D) were found to reduce tumor volume, viable cell count and increase non-viable cell count, and percentage increase in life span. All compounds showed significant activity in quenching DPPH free radical. Conclusion: All compounds showed significant (P<0.01) anticancer activity compared to EAC control group. All synthesized compound exhibited considerable antioxidant activity against free radicals, and they showed significant IC50 values and can, thus, ensure protection against oxidative stress.
Antinociceptive and Diuretic Activities of Tagetes erecta LinnJing Zang
In the present investigation, the possible antinociceptive and diuretic activities of methanolic extract of Tagetes erecta has been tested in animal models. The methanol extract of both aerial part and root of the plant exhibited significant antinociceptive activity at higher dose (400 mg/kg body weight) in Swiss albino mice. The root extract was found to reduce the writhing more effectively than that of aerial part which is comparable to that produced by aminopyrine, used as standard drug. In addition, crude whole plant extract was also showed efficient diuresis at higher dose 400 mg/kg tested. Diuretic activity was proved by the electrolyte loss ratio (Na+/K+ excretion ratio) and we used furosemide as the reference.
Acute and subchronic toxicity of mulberry fruitsEditya Apriliani
This document summarizes a study that investigated the acute and subchronic toxicity of mulberry fruits in rats. In the acute toxicity test, rats were given a single dose of 2000 mg/kg of mulberry fruit extract and observed for 14 days. No signs of toxicity or deaths were observed. In the subchronic toxicity test, rats were given oral doses of 2, 10, or 500 mg/kg/day of mulberry fruit extract for 90 days. Body weights, organ weights, hematological and clinical chemistry parameters were measured. No abnormalities were found except for some changes in BUN and glucose levels that were still within normal ranges. Histopathological examination also found no abnormalities. The results suggest mulberry fruits do not
Antidiabetic and Cytoprotective Effect of Ethanolic Extract of SalaciaNitida ...IOSRJPBS
The document summarizes a study that investigated the antidiabetic and cytoprotective effects of the ethanolic root extract of Salacia nitida on alloxan-induced diabetic rats. Key findings of the study include:
1) The ethanolic extract of S. nitida roots showed significant antidiabetic activity, demonstrated by a dose-dependent reduction in blood glucose levels and increase in body weight of treated diabetic rats.
2) Histological examination showed the extract helped restore damaged pancreatic and kidney tissues in treated diabetic rats closer to normal.
3) The extract demonstrated significant glucose tolerance effects and may lower blood glucose through mechanisms like stimulating insulin production or protecting pancreatic beta cells.
Analgesic activity of various leaf extracts of saraca indica linn.Younis I Munshi
The document summarizes a study that evaluated the analgesic activity of different leaf extracts of Saraca indica in mice models. The extracts showed dose-dependent analgesic effects in both the formalin-induced pain and tail immersion tests. In the formalin test, the methanol extract at 400 mg/kg produced the highest analgesic activity, inhibiting pain response by 55.87% in the first phase and 49.21% in the second phase. In the tail immersion test, the methanol extract also increased the tail flick latency time the most compared to control, suggesting it may have central analgesic effects. The results indicate that extracts of Saraca indica leaves possess analgesic properties.
Anti-inflammatory activity of Pupalia lappacea L.JussAnti inflammatory activi...pharmaindexing
This study evaluated the anti-inflammatory properties of the methanolic extract of Pupalia lappacea in carrageenan-induced rat paw edema and egg white-induced paw edema models. The extract at a dose of 200 mg/kg significantly inhibited paw edema in both models, supporting the traditional use of the plant for inflammatory conditions. HPTLC analysis revealed the presence of gallic acid, ferulic acid, chlorogenic acid, and rutin in the extract, which may be responsible for the anti-inflammatory effects. The results provide a pharmacological basis for the folkloric use of P. lappacea as an anti-inflammatory agent.
Hepatoprotective activity of extract of Homalium Letestui stem against carbon...oyepata
Hepatoprotective activity of extract of Homalium Letestui stem against
carbon tetrachloride-induced liver injury
Oyepata Simeon Joseph1*, Jude e Okokon2, Opeyemi tosin Joseph3
Background: Dennentia tripetalla (Pepper Fruit) belongs to the Annonaceae family and is abundant in Nigeria. Its
fruit in folklore medicine is used for treatment of varying ailments. While ample research evidence exists on the
plants fruit and seed, no current study exists on the toxicological profile of the plant leaves.
Methods: qualitative and quantitative phytochemicals and In vitro antioxidant assays were carried out using
standard methods. The acute toxicity study indicates that the LD50 was higher than 2000 mg/Kg body weight. Subchronic
toxicity studies was carried out using five groups of rats. Group 1 served as control, 2–5 received 100 mg/
Kg, 200 mg/Kg, 500 mg/Kg and 1000 mg/Kg body weight orally for 28 days.
Results: Post-administration biochemical analysis indicates there was increased weight in rats administered 100
mg/kg and 200 mg/kg while it reduced in the 500 mg/kg group. Significant elevations of liver function markers
were reported for 200 mg/kg and 500 mg/kg respectively. Serum and hepatic protein profiles remained unaltered.
Renal function analysis revealed elevated serum urea and creatinine for 200 and 500 mg/kg groups, elevated serum
Na+ and Ca+ and reduced serum Cl− for the 500 mg/Kg group. Elevated Kidney K+ and Ca+ levels, reduced Cl−
were significantly observed in 500 mg/Kg group. Significant rise in hepatic and renal lipid peroxidation was
observed in 200 and 500 mg/Kg groups. There were observed disarmament of the antioxidant defense systems
occasioned by rise and drop in tissue (hepatic, renal, testes, heart) Superoxide dismutase (SOD), Catalase (Cat),
Glutathione-s-transferase (GST), Glutathione peroxidase (GPx) activities in the test groups relative to control.
Histopathological examination indicated architectural aberrations at 500 and 1000 mg/kg.
Conclusions: It concluded that the plant had significant phytochemical and antioxidant properties of medical
interest and possessed toxic properties in rats when administered at a dose above 200 mg/Kg over a prolonged
period of time.
Toxicological profile of Grewia bicolor root extractIOSRJPBS
The document summarizes a study on the toxicological profile of Grewia bicolor root extract. The study found:
1) The tolerated dose of the extract in rats was 800mg/kg while the toxic dose was 1600mg/kg, with behavioral and neurological signs observed at toxic doses.
2) The median lethal dose (LD50) of the extract was calculated to be 2663.92 mg/kg in acute toxicity testing.
3) In a 28-day sub-acute toxicity study, rats administered 800mg/kg of the extract daily showed no changes in body weight, hematological, or biochemical parameters compared to controls.
Preliminary Studies on Antidiabetic, Antimicrobial and Antioxidant Activities...IJARIIT
Pharmaceutical industries are still in the hunt of effective scavengers for free radicals from the unexplored
medicinal plants. About 80,000 species of plants are utilized for treating various diseases in different systems of Indian
medicine. Many pharmaceutical companies giving importance in plant-derived drugs mainly due to the current widespread
belief that 'Green Medicine' is safe and more dependable than the costly synthetic drugs, which have adverse side effects.
The objective of the study is isolation of Phytochemical active constituents, Antidiabetic, antimicrobial and
antioxidant activities of the rare Antidiabetic medicinal plant Epaltes divaricata (Linn.) since the selected plant has varied
medicinal properties used in Ayurveda. This valuable plant is used in traditional Ayurvedic medicine to alleviate jaundice,
diabetes mellitus, urethral discharges and acute dyspepsia. It is also regarded as a diaphoretic, diuretic and a stimulating
expectorant.
The methanol extract of Epaltes divaricata L. showed excellent antimicrobial activity against bacteria and fungi.
Phytochemical analysis was carried out for the same extract by two different standard methods and which confirmed the
presence of steroids, triterpenoids and phenolic compounds. Decreasing of postprandial hyperglycemia is a therapeutic
approach for treating diabetes mellitus. This can be achieved in current trends through the inhibition of carbohydrate
hydrolyzing enzymes such as alpha glucosidase and alpha amylase. Agents with α-amylase and α-glucosidase inhibitory
activity are very useful as oral anti hypoglycemic agents for the control of hyperglycemia in patients who have diabetes
mellitus. In this study the methanol extract exhibited above 50% of inhibition in all standard concentration.
1) The aqueous extract of Sida rhombifolia ssp. retusa roots showed hepatoprotective effects against liver damage induced by thioacetamide and allyl alcohol in rats.
2) Pretreatment with the extract significantly reduced elevated liver enzymes and normalized liver weight and volume caused by thioacetamide toxicity. The extract also prevented decreases in body weight and reduced necrosis caused by allyl alcohol.
3) The study demonstrates that the roots of Sida rhombifolia ssp. retusa have potent hepatoprotective properties against chemically-induced liver damage in rats.
This document describes a study that evaluated the pharmacological effects of Terminalia chebula fruit extracts against neurodegenerative disorders in rodents. The study involved extracting Terminalia chebula fruits with polar and non-polar solvents, conducting preliminary phytochemical analysis, and screening the extracts for anti-parkinsonism and memory-enhancing effects. For anti-parkinsonism activity, the extracts were tested in a haloperidol-induced catatonia model in rats, while for memory effects the extracts were evaluated in ethanol-induced cognitive impairment and diazepam-induced amnesia models. The results of the study were discussed and compared to control groups.
Effects of Metformin, Pioglitazone and Aqueous Extract of Delonix Regia on Bl...iosrjce
The effects of Delonix regia extract (d200mg, d300mg, and d400mg), metformin (m8.3mg, m12.5mg
and m16.5mg), pioglitazone (p0.5mg, p0.7mg and p0.9mg) and combined formulation of metformin and extract
(m6.25d150mg) on glycated hemoglobin status in streptozotocin-induced diabetic Albino wistar rats. Diabetic
status of these rats was assessed by estimating fasting blood glucose levels. A total of 150 albino rats were used
for the investigation and were grouped into twelve groups of twelve rats each as follows; Group I: normal
control rats (NCR). Group II: Diabetic control rats (DCR). Group III: Diabetic rats treated with d200mg.
Group IV: Diabetic rats treated with d300mg. Group V: Diabetic rats treated with d400mg. Group VI: Diabetic
rats treated with m8.3mg. Group VII: Diabetic rats treated with m12.5mg. Group VIII: Diabetic rats treated
with m16.5mg. Group IX: Diabetic rats treated with p0.5mg. Group X: Diabetic rats treated with p0.75mg.
Group XI: Diabetic rats treated with p1.0mg. Group XII: Diabetic rats treated with m125d300mg each for male
and female respectively, for a total of 56 days. After every two weeks interval of treatment for eight weeks three
rats from each group were sacrificed and blood sample were collected and analyzed for various parameters.
The result obtained showed an elevated level of glycated hemoglobin in diabetic-induced wistar albino rats
compared with normal control rats. However, there was reversal of the effects when treated with the
drug/extract. Also there was reduction in the blood glucose level of the diabetic rats treated with metformin
(from 6.37±0.69 to 5.20±0.62mmol/l), pioglitazone (from 7.30±0.21mmol/l to 4.70±0.46), aqueous extract of
Delonixregia (from 8.20±0.81mmol/l to 6.10±0.60) and combined formulation of metformin and extract (from
7.81±0.34 to 4.80±0.17), at p<0.05 confidence level when compared with diabetic control rats in the various
weeks of treatment respectively
This research article studied the anti-obesity effects of Moringa oleifera leaf extract (MEMOL) in rats with high fat diet-induced obesity. Rats were fed a high fat diet to induce obesity over 49 days. Treatment with MEMOL for 49 days significantly reduced body weight, total cholesterol, triglycerides, and LDL levels in obese rats, and increased body temperature, compared to untreated obese rats. MEMOL treated rats also showed decreased levels of liver enzymes and blood glucose. The results indicate that MEMOL attenuated body weight gain in obese rats without affecting food intake, and demonstrated hypoglycemic and hypolipidemic effects, suggesting it may help treat obesity and related disorders.
This research article evaluated the potential aphrodisiac effects of Moringa oleifera seed extracts in male albino rats. Researchers administered aqueous, alcohol, and chloroform extracts of M. oleifera seeds to male rats for 21 days. They observed several parameters of sexual behavior and found that the extracts significantly increased mounting frequency, intromission frequency, and ejaculation latency, while decreasing mounting latency, intromission latency, and post-ejaculatory interval. The extracts also increased libido without any adverse effects. The results suggest that M. oleifera seed extracts can enhance sexual behavior in male rats, supporting its traditional use as an aphrodisiac.
The document evaluates the analgesic and anti-inflammatory activities of Karuvilanchi ver chooranam (KVC), the root powder of Smilax zeylanica, in rodent models. The results showed that KVC significantly increased the reaction time in hot plate test and inhibited acetic acid-induced writhing responses in mice, indicating its analgesic effects. KVC also significantly reduced formalin-induced paw edema in rats and cotton pellet-induced granuloma formation, demonstrating its anti-inflammatory properties. The study confirms the traditional use of KVC for pain and inflammation management.
The document summarizes a study that analyzed samples collected from diarrhea patients and their mothers in rural Bangladesh to detect coliform bacteria. Several species of coliform bacteria were isolated from the samples, with isolation rates ranging from 38.01-3.51%. Extracts of Moringa oleifera leaves were tested against the isolated bacteria. The leaf extracts showed antibacterial effects and were able to inhibit the growth of all tested bacterial pathogens, including E. coli, Shigella, Salmonella, Enterobacter, Klebsiella, and Serratia species. The extracts had zone of inhibition ranging from 8-23 mm and minimum inhibitory concentration values between 62.5-1000 μg/mL, suggesting M. oleifera extracts could
Background: The probable mechanism of an earlier reported capacity of palm oil extracts to confer protection
against high dose cadmium poisoning in rats was reported in this study. Similar experimental design earlier reported
by us was retained. Rats therefore were sacrificed at intervals of twelve; twenty four and forty eight hours post CdCl2
insult.
Results: Oxidative stress and antioxidant status (malondialdehyde, superoxide dismutase, catalase and glutathione)
were assessed in tissues (liver, kidney, heart, brain, muscle) and serum. Oxidative stress indicators showed a significantly
(p < 0.05) increased lipid peroxidation and alterations in antioxidant defence systems occasioned by drop in
catalase and superoxide dismutase enzymes (serum, liver, heart, brain and kidneys) of the rats. Also observed were
significant (p < 0.05) reduction in the non-enzymatic antioxidant reduced glutathione over time. Pre-administration of
rats with the crude palm oil and its extracts modulated cadmium mediated depletion of the antioxidant capacities of
rats acutely exposed to cadmium and rising lipid peroxidation profile.
Conclusions: Regulation of stress and antioxidant response was the underlying mechanism by which the extracts
conferred protection against high dose cadmium insult thus suggesting its potential as a viable therapeutic target
against its deleterious effects.
The use of medicinal plants in the treatment of harmful impacts of xenobiotics in animals is attracting an increasing attention in recent times. The aim of the current study is to assess the preventive potential of Costus afer aqueous leaves extract (CAAE) in treating metabolic aberrations imposed by crude oil contaminated diet in Wistar albino rats. Six groups of rats were treated as follows: A = Normal diet; B= Normal diet + 100 mg/kg body weight of CAAE; C =Normal diet + 200 mg/kg body weight of CAAE; D= Crude oil contaminated diet; E= crude oil contaminated diet + 100 mg/kg body weight of CAAE, F = crude oil contaminated diet + 200 mg/kg body weight of CAAE. After thirty days of exposure to the diet and administration of the corresponding plant extracts, the rats were sacrificed with chloroform and the required organs were excised. The hematological indices, as well as function indicators and levels of drug metabolizing enzymes in the liver and kidney, were investigated with standard protocols. The results indicated that the hematological parameters and kidney and liver function indices were altered in rats fed with crude oil contaminated diet. However, the values came close to those in control rats when Costus afer aqueous extracts were administered. Similarly, the activities of oxidase enzymes (aldehyde oxidase, monoamine oxidase, xanthine oxidase, and sulphite oxidase), following their inhibition by the ingestion of crude oil contaminated diet, equally restored close to control values upon treatment with Costus afer aqueous extract. This study, therefore, was able to establish an aqueous extract of Costus afer leave as an antidote for crude oil intoxication.
Blood glucose and lipid reducing activities of the oral administration of aqu...Alexander Decker
1. The study investigated the effects of repeated oral administration of an aqueous extract of Moringa oleifera leaves on blood glucose and lipid levels in male Wistar rats over 56 days.
2. The extract significantly reduced blood glucose and serum lipid levels like total cholesterol and triglycerides in a dose-dependent manner. It also significantly increased HDL cholesterol.
3. The extract maintained or slightly reduced body weight in the rats. It significantly reduced food intake, which may be due to effects on appetite or metabolism in the gastrointestinal tract.
Synthesis, Characterization, and Biological Evaluation of Some Novel Phthalim...BRNSS Publication Hub
Objective: Different Phthalimide derivatives (4,5,6,7-tetrachloro-2-[1,2,4]triazol-4-yl]-isoindole-1,3-dione derivatives) were synthesized and biological activities of them were evaluated. Materials and Methods: In the present study, four new phthalimide derivatives were synthesized. The structures of final compounds were characterized on the basis of spectral data. Then, biological evaluation of all the synthesized compounds means in vivo anticancer activity was evaluated on the Ehrlich ascites carcinoma (EAC) bearing Swiss albino mice model, and in vitro antioxidant activity was assessed using 1,1-diphenyl-2-picryl hydrazine (DPPH) radical scavenging assay. Results: The titled compounds (2A-2D) were found to reduce tumor volume, viable cell count and increase non-viable cell count, and percentage increase in life span. All compounds showed significant activity in quenching DPPH free radical. Conclusion: All compounds showed significant (P<0.01) anticancer activity compared to EAC control group. All synthesized compound exhibited considerable antioxidant activity against free radicals, and they showed significant IC50 values and can, thus, ensure protection against oxidative stress.
Antinociceptive and Diuretic Activities of Tagetes erecta LinnJing Zang
In the present investigation, the possible antinociceptive and diuretic activities of methanolic extract of Tagetes erecta has been tested in animal models. The methanol extract of both aerial part and root of the plant exhibited significant antinociceptive activity at higher dose (400 mg/kg body weight) in Swiss albino mice. The root extract was found to reduce the writhing more effectively than that of aerial part which is comparable to that produced by aminopyrine, used as standard drug. In addition, crude whole plant extract was also showed efficient diuresis at higher dose 400 mg/kg tested. Diuretic activity was proved by the electrolyte loss ratio (Na+/K+ excretion ratio) and we used furosemide as the reference.
Acute and subchronic toxicity of mulberry fruitsEditya Apriliani
This document summarizes a study that investigated the acute and subchronic toxicity of mulberry fruits in rats. In the acute toxicity test, rats were given a single dose of 2000 mg/kg of mulberry fruit extract and observed for 14 days. No signs of toxicity or deaths were observed. In the subchronic toxicity test, rats were given oral doses of 2, 10, or 500 mg/kg/day of mulberry fruit extract for 90 days. Body weights, organ weights, hematological and clinical chemistry parameters were measured. No abnormalities were found except for some changes in BUN and glucose levels that were still within normal ranges. Histopathological examination also found no abnormalities. The results suggest mulberry fruits do not
Antidiabetic and Cytoprotective Effect of Ethanolic Extract of SalaciaNitida ...IOSRJPBS
The document summarizes a study that investigated the antidiabetic and cytoprotective effects of the ethanolic root extract of Salacia nitida on alloxan-induced diabetic rats. Key findings of the study include:
1) The ethanolic extract of S. nitida roots showed significant antidiabetic activity, demonstrated by a dose-dependent reduction in blood glucose levels and increase in body weight of treated diabetic rats.
2) Histological examination showed the extract helped restore damaged pancreatic and kidney tissues in treated diabetic rats closer to normal.
3) The extract demonstrated significant glucose tolerance effects and may lower blood glucose through mechanisms like stimulating insulin production or protecting pancreatic beta cells.
Analgesic activity of various leaf extracts of saraca indica linn.Younis I Munshi
The document summarizes a study that evaluated the analgesic activity of different leaf extracts of Saraca indica in mice models. The extracts showed dose-dependent analgesic effects in both the formalin-induced pain and tail immersion tests. In the formalin test, the methanol extract at 400 mg/kg produced the highest analgesic activity, inhibiting pain response by 55.87% in the first phase and 49.21% in the second phase. In the tail immersion test, the methanol extract also increased the tail flick latency time the most compared to control, suggesting it may have central analgesic effects. The results indicate that extracts of Saraca indica leaves possess analgesic properties.
Anti-inflammatory activity of Pupalia lappacea L.JussAnti inflammatory activi...pharmaindexing
This study evaluated the anti-inflammatory properties of the methanolic extract of Pupalia lappacea in carrageenan-induced rat paw edema and egg white-induced paw edema models. The extract at a dose of 200 mg/kg significantly inhibited paw edema in both models, supporting the traditional use of the plant for inflammatory conditions. HPTLC analysis revealed the presence of gallic acid, ferulic acid, chlorogenic acid, and rutin in the extract, which may be responsible for the anti-inflammatory effects. The results provide a pharmacological basis for the folkloric use of P. lappacea as an anti-inflammatory agent.
Hepatoprotective activity of extract of Homalium Letestui stem against carbon...oyepata
Hepatoprotective activity of extract of Homalium Letestui stem against
carbon tetrachloride-induced liver injury
Oyepata Simeon Joseph1*, Jude e Okokon2, Opeyemi tosin Joseph3
Background: Dennentia tripetalla (Pepper Fruit) belongs to the Annonaceae family and is abundant in Nigeria. Its
fruit in folklore medicine is used for treatment of varying ailments. While ample research evidence exists on the
plants fruit and seed, no current study exists on the toxicological profile of the plant leaves.
Methods: qualitative and quantitative phytochemicals and In vitro antioxidant assays were carried out using
standard methods. The acute toxicity study indicates that the LD50 was higher than 2000 mg/Kg body weight. Subchronic
toxicity studies was carried out using five groups of rats. Group 1 served as control, 2–5 received 100 mg/
Kg, 200 mg/Kg, 500 mg/Kg and 1000 mg/Kg body weight orally for 28 days.
Results: Post-administration biochemical analysis indicates there was increased weight in rats administered 100
mg/kg and 200 mg/kg while it reduced in the 500 mg/kg group. Significant elevations of liver function markers
were reported for 200 mg/kg and 500 mg/kg respectively. Serum and hepatic protein profiles remained unaltered.
Renal function analysis revealed elevated serum urea and creatinine for 200 and 500 mg/kg groups, elevated serum
Na+ and Ca+ and reduced serum Cl− for the 500 mg/Kg group. Elevated Kidney K+ and Ca+ levels, reduced Cl−
were significantly observed in 500 mg/Kg group. Significant rise in hepatic and renal lipid peroxidation was
observed in 200 and 500 mg/Kg groups. There were observed disarmament of the antioxidant defense systems
occasioned by rise and drop in tissue (hepatic, renal, testes, heart) Superoxide dismutase (SOD), Catalase (Cat),
Glutathione-s-transferase (GST), Glutathione peroxidase (GPx) activities in the test groups relative to control.
Histopathological examination indicated architectural aberrations at 500 and 1000 mg/kg.
Conclusions: It concluded that the plant had significant phytochemical and antioxidant properties of medical
interest and possessed toxic properties in rats when administered at a dose above 200 mg/Kg over a prolonged
period of time.
Toxicological profile of Grewia bicolor root extractIOSRJPBS
The document summarizes a study on the toxicological profile of Grewia bicolor root extract. The study found:
1) The tolerated dose of the extract in rats was 800mg/kg while the toxic dose was 1600mg/kg, with behavioral and neurological signs observed at toxic doses.
2) The median lethal dose (LD50) of the extract was calculated to be 2663.92 mg/kg in acute toxicity testing.
3) In a 28-day sub-acute toxicity study, rats administered 800mg/kg of the extract daily showed no changes in body weight, hematological, or biochemical parameters compared to controls.
Preliminary Studies on Antidiabetic, Antimicrobial and Antioxidant Activities...IJARIIT
Pharmaceutical industries are still in the hunt of effective scavengers for free radicals from the unexplored
medicinal plants. About 80,000 species of plants are utilized for treating various diseases in different systems of Indian
medicine. Many pharmaceutical companies giving importance in plant-derived drugs mainly due to the current widespread
belief that 'Green Medicine' is safe and more dependable than the costly synthetic drugs, which have adverse side effects.
The objective of the study is isolation of Phytochemical active constituents, Antidiabetic, antimicrobial and
antioxidant activities of the rare Antidiabetic medicinal plant Epaltes divaricata (Linn.) since the selected plant has varied
medicinal properties used in Ayurveda. This valuable plant is used in traditional Ayurvedic medicine to alleviate jaundice,
diabetes mellitus, urethral discharges and acute dyspepsia. It is also regarded as a diaphoretic, diuretic and a stimulating
expectorant.
The methanol extract of Epaltes divaricata L. showed excellent antimicrobial activity against bacteria and fungi.
Phytochemical analysis was carried out for the same extract by two different standard methods and which confirmed the
presence of steroids, triterpenoids and phenolic compounds. Decreasing of postprandial hyperglycemia is a therapeutic
approach for treating diabetes mellitus. This can be achieved in current trends through the inhibition of carbohydrate
hydrolyzing enzymes such as alpha glucosidase and alpha amylase. Agents with α-amylase and α-glucosidase inhibitory
activity are very useful as oral anti hypoglycemic agents for the control of hyperglycemia in patients who have diabetes
mellitus. In this study the methanol extract exhibited above 50% of inhibition in all standard concentration.
1) The aqueous extract of Sida rhombifolia ssp. retusa roots showed hepatoprotective effects against liver damage induced by thioacetamide and allyl alcohol in rats.
2) Pretreatment with the extract significantly reduced elevated liver enzymes and normalized liver weight and volume caused by thioacetamide toxicity. The extract also prevented decreases in body weight and reduced necrosis caused by allyl alcohol.
3) The study demonstrates that the roots of Sida rhombifolia ssp. retusa have potent hepatoprotective properties against chemically-induced liver damage in rats.
This document describes a study that evaluated the pharmacological effects of Terminalia chebula fruit extracts against neurodegenerative disorders in rodents. The study involved extracting Terminalia chebula fruits with polar and non-polar solvents, conducting preliminary phytochemical analysis, and screening the extracts for anti-parkinsonism and memory-enhancing effects. For anti-parkinsonism activity, the extracts were tested in a haloperidol-induced catatonia model in rats, while for memory effects the extracts were evaluated in ethanol-induced cognitive impairment and diazepam-induced amnesia models. The results of the study were discussed and compared to control groups.
This study evaluated the safety and anti-diabetic effects of a polyherbal phytocomposite (PHC) created from the leaves of Ficus benghalensis, Syzygium cumini, and Ocimum sanctum. In acute toxicity tests, the PHC showed no adverse effects at doses up to 7.5g/kg and the median lethal dose was determined to be 20.25g/kg. In sub-chronic toxicity tests, the PHC at doses up to 750mg/kg caused some changes in organ weights and blood biomarkers but no mortality. The PHC significantly lowered blood glucose and lipids in diabetic rats, performing better than the individual plant extracts and metformin. The PHC
Hepatoprotective Activity of Methanolic Extract of Whole Plant of Pulicaria W...IOSRJPBS
Natural remedies from medicinal plants are considered to be effective and safe alternative treatment for liver injury. The present study was conducted to evaluate the hepatoprotective activity of methanolic extract of whole plant of Pulicaria wightiana in wistar rats. The studies were conducted using the two popular inducing agents Paracetamol (2 g/kg, p.o.) in 1% NaCMC and Carbon tetrachloride (1 ml/kg). Silymarin (100 mg/kg, p.o.) was used as reference drug in the respective models. The effect was estimated by measuring the enzymatic levels and histo- pathological studies. The methanolic extract of whole plant of Pulicaria wightiana has shown very significant hepatoprotection against both Paracetamol and CCl4 - induced hepatotoxicity study models in wistar rats. This was evidenced by marked reduction in marker enzymes in serum. Histopathological studies also confirmed the hepatoprotective nature of the extract
Evaluation of antinociceptive effect of Terminalia arjuna bark ethanol extractOpen Access Research Paper
Stem bark of the plant Terminalia arjuna were extracted in 50% ethanol to evaluate for centrally acting analgesic potential using formalin, hot plate and peripheral pharmacological actions using acetic acid induced writhing test in mice. The extract of the plant were found to have significant (p<0.01) analgesic activity at the oral dose of 250 & 500 mg/kg body weight, in the tested models. In hot plate test, at both dose levels (250mg/kg and 500mg/kg), T. arjuna extract showed significant (p<0.001) increased latency period than the control group. In acetic acid induced writhing test and formalin test T. arjuna also showed reduced number of writhes than the control group at two dose levels which are significant (p<0.05) compared to control. The results obtained support the use of stem bark of T.arjuna in painful conditions acting both centrally and peripherally.
DPPH Scavenging Assay of Eighty Four Bangladeshi Medicinal PlantsIOSR Journals
This study was designed to screen out free radical scavenging potentiality of 84 medicinal plants. Stock solution of different plant extracts and standard were diluted to achieve suitable concentrations. A control was also prepared without plant extract solution. Then 0.004% DPPH solution was added. The mixtures were incubated in the room temperature for 30 minutes. Then the absorbance was measured at 517 nm against solvent in UV-spectrophotometer and then IC50 was calculated. In this experiment two standard were used-ascorbic acid and BHT. Both showed a significant IC50 value of 15.5μg/mL, and 46.54μg/mL respectively. Among 84 medicinal plants Syzygim cumini, Casuarina littorea, Borassus flabellifer, Enhydra fluctuans, and Minusops elengi exhibited highest radical scavenging potential with an IC50 value of 12.816μg/mL, 14.467μg/mL, 15.755μg/mL, 15.653μg/mL, and 20.380μg/mL respectively. All these value are very close to the IC50 value of ascorbic acid and better than IC50 value of BHT (Butylated Hydroxy Toluene). Syzygim cumini is the most powerful scavenger among all tested medicinal plants and also most strong scavenger than ascorbic acid and BHT. Scavenging activity was found to increase in dose dependent manner. Another 30 medicinal plants exhibited good scavenging property and 14 medicinal plants showed moderate scavenging activity. The rest presented lower scavenging activity. This present study indicates that plants having good scavenging property may have various health beneficial effects and these plants can be considered as valuable source of bioactive components with high antioxidant properties.
1) The document reports on a pre-clinical study that investigated the effects of Aegle marmelos (bael) leaf extract on blood glucose levels in normal and diabetic mice.
2) 32 mice were divided into groups: normal mice given normal feed, normal mice given bael extract, diabetic mice given normal feed, and diabetic mice given bael extract. Diabetes was induced via alloxan injection.
3) Blood samples were taken from the mice at various intervals and blood glucose was measured. It was found that bael extract was effective in reducing and maintaining normal blood glucose levels in both normal and hyperglycemic mice.
1) The study investigated the effects of Clitoria ternatea leaf and flower extracts on alloxan-induced diabetic rats over 84 days.
2) Oral administration of the extracts significantly reduced blood glucose, cholesterol, triglycerides, and kidney/liver markers, while increasing insulin, glycogen stores, and beneficial cholesterol.
3) The leaf extract showed slightly better effects than the flower extract in regulating these biochemical parameters and enzymes related to glucose metabolism.
ANTIDIABETIC POTENTIAL OF MEMECYLON TERMINALE DALZ EXTRACTS IN ALLOXAN INDUCE...Jing Zang
Memecylon terminale Dalz is an endemic plant that is exclusively present in the Western Ghat region of Southern India. The traditional healers in this region have been using this plant extract to cure disorders such as dysentery, diabetes, diarrhea, piles, haemoptysis, menorrhagia and shown to possess carminative stomachic astringent property. The present study was carried out to evaluate the hypoglycemic activity of different extracts of M. terminale Dalz in alloxan induced diabetic rats. Chloroform and methanolic extracts of M. terminale Dalz leaves were screened for hypoglycemic activity in alloxan induced diabetes in Swiss albino rats. The lipid profile was also examined in diabetic rats administered with these extracts. The efficacy of these extracts was also analyzed for their ability to inhibit α-glucosidase and α-amylase. Of the two extracts, chloroform extract (500 mg/kg body weight) showed dose dependent decrease in blood glucose level which was comparable to that of control after 24 h of extract administration. On the other hand, chloroform extract also showed good lipid profile that was comparable with standard drug. As compared to chloroform extract, the methanolic extract showed good inhibition of α-amylase and α-glucosidase enzyme activity. The results of our study indicate that M. terminale Dalz plant possesses significant protective effects against alloxan induced diabetes. However, detailed structure function analysis of active ingredients of the extract is needed to be validated.
In vivo evaluation of antidiarrhoeal activity of ethanolic extract of leaf an...Open Access Research Paper
Ficus carica Linn is occasionally used in preparation of local traditional medicines used in the treatment of diarrhoea in Bangladesh. Our present studies make an attempt toward validating this traditional use by investigating antidiarrhoeal activity of F. carica Linn. Ethanolic extract of leaf and bark of F. carica Linn showed significant (p ≤ 0.05) decrease in the severity of diarrhoea, in a dose dependent manner, in castor oil induced diarrhoea test. Prostaglandin E2 induced intestinal fluid accumulation test (enteropooling) gave significant results (P ≤ 0.05), indicating possible antidiarrhoeal action. The extract produced significant (P ≤ 0.05) reduction of intestinal transit in gastrointestinal motility test with barium sulfate milk in healthy rats. It is evident that F. carica Linn have significant antidiarrhoeal activity and may be a potential source of antidiarrhoeal agents.
Analgesic and anti-inflammatory activity of methanol extract of Xanthosoma sa...Uploadworld
Xanthosoma sagittifolium is widely used in the folkloric treatment system of Bangladesh for the treatment of different diseases. The present study was conducted to evaluate the analgesic and antiinflammatory potentiality of methanol extract of Xanthosoma sagittifolium in mice.
This document describes a study evaluating the analgesic and anti-inflammatory properties of the methanol extract of Xanthosoma sagittifolium. In the tail immersion test for analgesic activity, the plant extract at 200 mg/kg body weight showed the highest reaction time of 8.25 seconds after 120 minutes, compared to 4 seconds for the control. In the acetic acid-induced writhing test, the plant extract at 200 μg/ml produced significant pain inhibition of 84.48%. For anti-inflammatory activity in the carrageenan-induced paw edema test, the highest inhibition of 74.77% was found for the plant extract at a dose of 200μg/ml after 120 minutes. The results
Glucose lowering potential of hydromethanolic extract of RauwolfiaJing Zang
The objective of the present study was to evaluate the phytochemistry, acute toxicity and glucose lowering potential of hydromethanolic roots extract (HMREt) of Rauwolfia serpentina. The qualitative analysis of HMREt showed the presence of many important phyto-constituents except anthraquinones, carbohydrates and saponins whereas quantitatively it found rich in total phenols. In acute toxicity study, orally administrated HMREt from 5-250 mg/ kg was observed safe and non-sedative while its doses from 500-2500 mg/kg were found sedative and induced mortalities (17-100%) within 4 hours of administration. The median lethal dose (LD50) of same extract was calculated as 1412.54 mg / kg (log LD50 = 3.15 mg/ kg) from log doses verses probit graph. The HMREt in doses of 50, 100 and 150 mg/kg induced significant percent decrease in blood glucose level at 30, 60 and 120 minutes in normo-hyperglycemic test mice as compared to control and negative control groups (p<0.05). The results concluded that HMREt has glucose lowering potential either by developing glucose tolerance or by pancreatic action in normo-hyperglycemic mice.
IOSR Journal of Pharmacy (IOSRPHR), www.iosrphr.org, call for paper, research...iosrphr_editor
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Hepatoprotective Activity of Cinnamon Zeylanicum Leaves against Alcohol Induc...IJERA Editor
Plants play an important role in the life of human, as the major source of food, as well as for the maintenance and improvement of health and for the elimination of the enemies since ages. Plants are the basic source of knowledge of modern medicine. The present study was conducted to evaluate the hepatoprotective activity of aqueos extract of aerial parts of Cinnamon zeylanicum are evaluated in alcohol induced hepatotoxicity in albino rats. Silymarin (100mg/kg) was given as reference standard. The aqueos extract of aerial parts of Cinnamon zeylanicum have shown very significant hepatoprotection against alcohol induced hepatotoxicity in albino rats in reducing SGOT, SGPT, Alkaline phosphatase (ALP) and GGT and levels of total bilirubin and total protein were investigated and showed an increase in alcohol induced rats when compared to control. The extracts of the test plant exhibited significant (p < 0.05) hepatoprotective activity against the alcohol induced liver models by improving liver function which was indicated by reduction in the levels of SGOT, SGPT, ALP, GGT, total bilirubin and total protein.
This study compared the antidiabetic effects of methanolic extracts of leaves from Abroma augusta and seeds from Syzygium cumini in alloxan-induced diabetic rats. Rats were treated with either plant extract at 300 mg/kg body weight daily for 7 days. Treatment with both plant extracts significantly reduced blood glucose levels and increased body weight compared to untreated diabetic rats. Histopathological analysis also showed improvements in treated rats compared to changes seen in untreated diabetic rats. The results indicate that both Abroma augusta and Syzygium cumini have antidiabetic properties and warrant further study of their active components for potential antidiabetic drug development.
This study compared the antidiabetic effects of methanolic extracts of Abroma augusta leaves and Syzygium
cumini seeds in alloxan-induced diabetic rats. Rats were treated with 300 mg/kg of each extract daily for 7
days. Both extracts significantly reduced blood glucose levels and increased body weight compared to
untreated diabetic rats. Histopathological analysis also showed protective effects on tissues. The extracts
were found to have antidiabetic properties similar to the standard drugs metformin and glibenclamide,
suggesting their potential as antidiabetic agents.
The document summarizes a study that evaluated the total tannin content and antidiarrheal activity of Tecomaria capensis leaves extract. The study found that the extract contained high quantities of tannins. In tests using animal models, the extract showed significant and dose-dependent inhibition of diarrhea, reduction of intestinal transit, and antienteropooling effects, supporting the traditional use of the plant for treating diarrhea. The results provide scientific evidence that Tecomaria capensis leaves extract has potential antidiarrheal properties.
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1. 1302
Advances in Natural and Applied Sciences, 6(8): 1302-1306, 2012
ISSN 1995-0772
This is a refereed journal and all articles are professionally screened and reviewed
ORIGINAL ARTICLE
Corresponding Author: Dr. Mohammed Rahmatullah, Pro-Vice Chancellor and Dean, Faculty of Life Sciences, University
of Development Alternative, House 78, Road 11A, Dhanmondi, Dhaka-1205, Bangladesh
Tel: 88-02-9136285; Fax: 88-02-8157339; E-mail: rahamatm@hotmail.com
Evaluation Of Antihyperglycemic And Antinociceptive Activities Of Methanolic Extract
Of Kaempferia Rotunda L. (Zingiberaceae) Rhizomes
Zakia Sultana, Khandaker Md. Sharif Uddin Imam, Fardous Mohammad Safiul Azam,
Shahnaz Rahman, Shiblur Rahman, Fatema Islam, Mohammed Rahmatullah
Department of Biotechnology & Genetic Engineering, Faculty of Life Sciences, University of Development
Alternative, Dhanmondi, Dhaka-1205, Bangladesh.
Zakia Sultana, Khandaker Md. Sharif Uddin Imam, Fardous Mohammad Safiul Azam, Shahnaz
Rahman, Shiblur Rahman, Fatema Islam, Mohammed Rahmatullah: Evaluation Of Antihyperglycemic
And Antinociceptive Activities Of Methanolic Extract Of Kaempferia Rotunda L. (Zingiberaceae)
Rhizomes
ABSTRACT
The objective of this study was to conduct antihyperglycemic and antinociceptive activity studies with
methanolic extract of rhizomes of Kaempferia rotunda, a plant used in folk medicines of Bangladesh for
treatment of high blood sugar level and pain. Antihyperglycemic activity studies were conducted in glucose-
loaded mice in oral glucose tolerance tests. Mice were given various doses of extract, followed by glucose
(2g/kg body weight), 60 min after administration of extract. Serum glucose levels were measured 120 minutes
after glucose administration. Antinociceptive activity studies were conducted in intraperitoneally acetic acid
injected mice through measurement of reductions in abdominal writhings caused by acetic acid-induced gastric
pain. Following a period of 1 h after oral administration of various doses of extract, all mice received
intraperitoneal injection of 1% acetic acid at a dose of 10 mL/kg body weight. To ensure bio-availability of
acetic acid, a period of 5 min was given to each animal following which period the number of writhing times
was counted for 10 min. The extract caused dose-dependent significant lowering in serum glucose levels in mice
(P<0.05), when administered at doses of 50, 100, 200 and 400 mg per kg body weight to glucose-loaded mice as
compared to control animals. Highest lowering of serum glucose (39.6%) was observed at an extract dose of 400
mg. In comparison, a standard antihyperglycemic drug, glibenclamide, when administered at a dose of 10 mg
per kg body weight, lowered serum glucose levels by 42.4%. The extract also demonstrated dose-dependent
significant antinociceptive activity (P<0.05), when administered to mice compared to control animals. At a dose
of 400 mg extract per kg body weight, the number of abdominal writhings was inhibited by 69.4% as compared
to 73.4% inhibition obtained with a standard antinociceptive drug, aspirin, administered at a dose of 400 mg per
kg body weight. The significant antihyperglycemic and antinociceptive activity demonstrated by the extract
validates the use of rhizomes of K. rotunda in folk medicines of Bangladesh for treatment of high blood sugar
levels as commonly observed in diabetic patients, and pain, and merits further scientific studies leading to
identification of relevant bioactive constituents.
Key words: Kaempferia rotunda, Zingiberaceae, antihyperglycemic, antinociceptive
Introduction
Kaempferia rotunda L. (Family: Zingiberaceae, English name: Indian crocus, Local name: Misri dana) is a
plant that can be found in the Indian sub-continent including various parts of Bangladesh. The plant is used in
the folk medicinal system of Bangladesh for treatment of high blood sugar levels as commonly observed in
diabetic patients, as well as for treatment of pain. For treatment of high blood sugar levels and pain,
respectively, juice obtained from macerated rhizomes is orally and topically administered. The plant is
considered to be an important medicinal plant also in the ancient traditional medicinal system of India, namely,
Ayurveda. The Ayurvedic drug, hallakam, containing tubers of the plant is considered as stomachic,
antiinflammatory to wounds and bruises, and useful for treatment of mental disorders and insomnia (Sereena et
al., 2011).
Methanol extracts of rhizomes of the plant reportedly contained six polyoxygenated cyclohexane
derivatives identified as (-)-6-acetylzeylenol (I), four acylated derivatives of 1-benzoyloxymethyl-1,6-
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epoxycyclohexan-2,3,4,5-tetrol (III-VI), a Diels-Alder adduct of 3-benzoyl-1-benzoyloxymethylcyclohexa-4,6-
dien-2,3-diol (VII), and a triacylated derivative of salicin (IX), in addition to (-)-zeylenol (II), and crotepoxide
(VIII). Compounds II and VI demonstrated antifeedant activities against larvae of Spodoptera littoralis, the
Egyptian cotton leafworm (Stevenson et al., 2007). The antioxidant potential of the plant has been evaluated and
flavonoids and phenolic derivatives found in the plant, which demonstrated inhibitory potential against lipid
peroxidation, suggesting that the plant can be useful in diseases like myocardial infarction, diabetes mellitus,
hepatic injury, atherosclerosis, rheumatoid arthritis, and cancer (Priya Mohanty et al., 2008). Two compounds
has been identified from rhizomes of the plant, namely, 2’-hydroxy’-4,4’,6’-trimethoxy-chalcone and (+)-
crotepoxide, the first possessing antioxidant activity (Lotulung et al., 2008). A lectin (designated as KRL) has
been purified from rhizomes of the plant, which demonstrated antibacterial activity and antiproliferative activity
against Ehrlich ascites carcinoma cells (Kabir et al., 2011). Alcoholic extract of rhizomes reportedly showed
anthelmintic activity against Pheretima posthuma and Ascardia galli (Agrawal et al., 2011).
High blood sugar level is one of the manifestations of diabetes, a disease which afflicts millions of people
worldwide and is rapidly reaching endemic proportions because of changes in human life style and dietary
habits. The disease cannot be cured by allopathic medicine and can rapidly progress to develop other
complications like cardiovascular disorders, diabetic retinopathy, diabetic neuropathy, and diabetic nephropathy.
Pain is also a problem afflicting millions of people worldwide on a daily basis and can arise from simple causes
like bruises or complicated causes like rheumatoid arthritis or cancer. In many of these diseases, the patient has
to take pain-killer drugs like aspirin or paracetamol for long stretches of time. These medicines can cause side-
effects like gastric ulceration and hepatic damages, respectively, following particularly any over-dosage. The
folk medicinal practitioners of Bangladesh (Kavirajes) claim to have effective treatments for both diabetes and
pain without resultant toxicity as occurs with allopathic pain killers. We had been screening the various
antidiabetic and antinociceptive medicinal plants used by the Kavirajes for some time to determine their actual
efficacies for the purposes that they are used for by the folk medicinal practitioners (Khan et al., 2010; Rahman
et al., 2010 a,b; Rahman et al., 2011). The objective of the present study was to evaluate the antihyperglycemic
and antinociceptive activities of methanolic extract of K. rotunda rhizomes.
Materials and Methods
1.1 Plant materials and extraction:
Rhizomes of K. rotunda were collected from Dinajpur district, Bangladesh in April 2011. The plant was
taxonomically identified by the Bangladesh National Herbarium at Dhaka (Accession Number: 63036).
Rhizomes were air-dried in the shade for one week, grounded into a fine powder, and were extracted with
methanol at a ratio of 1:6 (weight/volume) for 48 hours. The initial weight of dried powder used for extraction
was 50 g; the final weight of the methanol extract obtained from the dried powder was 5.935 g (yield =
11.87%).
1.2 Chemicals and drugs:
Glibenclamide, aspirin and glucose were obtained from Square Pharmaceuticals Ltd., Bangladesh. All other
chemicals were of analytical grade.
1.3 Animals:
In the present study, Swiss albino mice (male), which weighed between 15 to 20 g were used. The animals
were obtained from International Centre for Diarrheal Disease Research, Bangladesh. All animals were kept
under ambient temperature with 12 h light followed by a 12 h dark cycle. The animals were acclimatized for 3
days prior to actual experiments. The study was conducted following approval by the Institutional Animal
Ethical Committee of the University of Development Alternative, Dhaka, Bangladesh.
1.4 Antihyperglycemic activity:
Glucose tolerance property of methanol extract of whole plants was determined as per procedure previously
described by Joy and Kuttan (Joy and Kuttan, 1999) with minor modifications. In brief, fasted mice were
grouped into six groups of six mice each. The various groups received different treatments. Group 1 received
vehicle (1% Tween 80 in water, 10 mL/kg body weight) and served as the normal control; group 2 received
standard drug (glibenclamide, 10 mg/kg body weight); groups 3 to 6 received methanol extract of K. rotunda
rhizomes at doses of 50, 100, 200 and 400 mg/kg body weight, respectively. Doses were prepared by thoroughly
suspending methanol extract in 1% Tween 80 in water. Each mouse was weighed and dose-adjusted accordingly
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prior to administration of vehicle, standard drug, and test samples. All substances were orally administered.
Following a period of 1 h, all mice were orally administered 2 g/kg glucose. Blood samples were collected 2 h
after the glucose administration through puncturing heart. Serum glucose levels were measured by glucose
oxidase method (Venkatesh et al., 2004).
1.5 Antinociceptive activity:
Antinociceptive activity of methanol extract of K. rotunda whole rhizomes was examined using previously
described procedures (Shanmugasundaram and Venkataraman, 2005). Briefly, mice were divided into seven
groups of six mice each. Group 1 served as normal control and was administered vehicle only; groups 2 and 3
were orally administered standard antinociceptive drug, aspirin at doses of 200 and 400 mg/kg body weight,
respectively. Groups 4 to 7 were administered methanolic rhizome extract of K. rotunda at doses of 50, 100, 200
and 400 mg/kg body weight, respectively. Following a period of 1 h after oral administration of standard drug or
extract, all mice received intraperitoneal injection of 1% acetic acid at a dose of 10 mL/kg body weight. To
ensure bio-availability of acetic acid, a period of 5 min was given to each animal following which period the
number of writhing times was counted for 10 min.
1.6 Statistical analysis:
Experimental values are expressed as mean ± SEM. Independent Sample t-test was carried out for statistical
comparison. Statistical significance was considered to be indicated by a p value < 0.05 in all cases.
Results:
2.1 Antihyperglycemic activity:
In oral glucose tolerance tests conducted with glucose-loaded mice, the methanolic extract demonstrated
significant dose-dependent antihyperglycemic activity. The standard antihyperglycemic drug, glibenclamide,
following administration reduced glucose level significantly by 42.4% (P<0.05). At all doses except the 50 mg
per kg body weight dose, the extract caused significant reductions in serum glucose levels as compared to
control group (P<0.05). The percent reductions in serum glucose levels at doses of 50, 100, 200 and 400 mg/kg
body weight were, respectively, 16.2, 31.2, 38.1, and 39.6. Thus even at a dose of 200 mg extract, the results
compare favorably to that of glibenclamide. This suggests that the extract has potent antihyperglycemic
properties, and merits further consideration for isolation of bioactive constituent(s) responsible for the observed
antihyperglycemic effect. The results are shown in Table 1 (Part A).
Table 1: Antihyperglycemic and antinociceptive evaluation of methanolic extract of rhizomes of K. rotunda in mice.
A. Antihyperglycemic activity
Treatment Plant part used Dose (mg/kg body
weight)
Serum glucose level
(mmol/L)
% lowering of
serum glucose
level
Control (Group 1) - 10 ml 7.67 ± 0.55 -
Glibenclamide (Group 2) - 10 mg 4.42 ± 0.10* 42.4
K. rotunda (Group 3) Rhizome 50 mg 6.43 ± 0.47 16.2
K. rotunda (Group 4) Rhizome 100 mg 5.28 ± 0.27* 31.2
K. rotunda (Group 5) Rhizome 200 mg 4.75 ± 0.41* 38.1
K. rotunda (Group 6) Rhizome 400 mg 4.63 ± 0.37* 39.6
B. Antinociceptive activity
Treatment Plant part used Dose (mg/kg body
weight) Mean number of writhings
% inhibition
Control (Group 1) - 10 ml 8.17 ± 0.79 -
Aspirin (Group 2) - 200 mg 4.00 ± 0.58* 51.0
Aspirin (Group 3) - 400 mg 2.17 ± 0.98* 73.4
K. rotunda (Group 4) Rhizome 50 mg 4.00 ± 1.03* 51.0
K. rotunda (Group 5) Rhizome 100 mg 3.33 ± 0.95* 59.2
K. rotunda (Group 6) Rhizome 200 mg 3.17 ± 0.87* 61.2
K. rotunda (Group 7) Rhizome 400 mg 2.50 ± 0.99* 69.4
All administrations were made orally. Values represented as mean ± SEM, (n=6); *P < 0.05, significant compared to control animals. Note
that antihyperglycemic activity and antinociceptive activity studies were carried out with separate groups of animals.
2.2 Antinociceptive activity:
The extract also showed significant dose-dependent reductions in the number of abdominal writhings in
mice, which were induced through intraperitoneal administration of acetic acid in the antinociceptive activity
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test. At all doses of the extract tested, there were significant reductions in the number of abdominal writhings,
when compared to control group of animals (P<0.05). At the highest dose of extract tested (400 mg), the number
of writhings was reduced by 69.4%. A standard antinociceptive drug, aspirin, reduced the number of writhings
by 51.0 and 73.4%, respectively, when administered at doses of 200 and 400 mg per kg body weight. The results
are shown in Table 1 (Part B). Even at the lowest dose of the extract (50 mg per kg body weight), the reduction
in the number of writhings compare favorably to that of aspirin, when administered at a dose of 200 mg per kg
body weight. At the highest dose of extract tested (400 mg per kg body weight), the number of writhings
compare favorably to that of aspirin, even when administered at the higher dose of 400 mg per kg body weight.
Discussion:
It is to be noted that antihyperglycemic and antinociceptive activities of the extract were measured in two
separate groups of animals. This is standard procedure as reported in the scientific literature, for the two
procedures for measurement of the two activities are different. Complications could ensue and erroneous results
obtained if one is to do the same set of experiments on the same group of animals. For instance, the pain created
in mice by intraperitoneal administration of acetic acid and the consequent writhings may affect levels of blood
sugar in the experimental animals, and which may be erroneously attributed to the extract. However, the fact
that the extract had both antihyperglycemic as well as antinociceptive activities (as separately determined) can
be utilized in treatment of pain in hyperglycemic animals (including humans) as well as used separately for
treatment of hyperglycemia (which can arise from diabetes) or pain. Notably, this applies to the extract only. If
antihyperglycemic and antinociceptive activities are due to different phytochemical constituents of the extract
(as is possible), then administration of isolated responsible phytochemical(s) will demonstrate only one type of
activity.
The observed lowering of blood sugar by the extract may be achieved through various individual
mechanisms or a combination of mechanisms. The extract may have potentiated pancreatic secretion of insulin,
increased glucose uptake from serum, or decreased glucose absorption from gut (Farjou et al., 1987; Nyunai et
al., 2009; Bhowmik et al., 2009). Studies are now being actively pursued in our laboratory to isolate the
bioactive phytochemical(s) responsible for the observed antihyperglycemic activity.
It has been reported that acetic acid-induced writhing test can detect both central and peripheral analgesia
(Shanmugasundaram and Venkataraman, 2005). Intraperitoneal administration of acetic acid leads to occurrence
of pain, which is caused mainly through increased production of prostacyclines and prostaglandin E leading to
excitation of the A -nerve fibers and so causing sensation of pain (Reynolds, 1982). Therefore any compound
that lowers the number of abdominal constrictions will be demonstrating analgesia by inhibition of
prostaglandin synthesis. It is possible that the extract contains such pain-relieving components and it is under
investigation whether the extract does contain such components responsible for the antinociceptive effects.
The results from the oral glucose tolerance tests (for antihyperglycemic effects) and the acetic acid-induced
writhing tests (for antinociceptive effect) validate the use of rhizomes of K. rotunda by folk medicinal
practitioners for treatment of high blood sugar levels (as in diabetic patients) and for the relief of pain due to
various causes like bruises, rheumatoid arthritis, and headache. It can be expected that more screening of the
medicinal plants of Bangladesh and scientific validation of their folk medicinal uses can not only alleviate
patient’s sufferings through availability of cheaper medicines but also spur conservation efforts through
cultivation and conservation of such medicinal plants. Chinese traditional medicine, utilizing medicinal plants,
has now gained worldwide recognition through scientific validation of numerous formulations used traditionally
for centuries for the treatment of various ailments. This has benefited all human beings of the world. It is hoped
that the medicinal plants of Bangladesh can have a similar effect in alleviating various diseases of human
beings.
Financial support:
The study was supported by internal funding from the University of Development Alternative.
Competing interests:
The authors declare that they have no competing interests.
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