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IndoorAir2014_BioBE
- 1. What’s In YourPersonal Microbial Cloud?! James Meadow! Postdoctoral Research Associate ! Biology and the Built Environment! University of Oregon! ! jfmeadow@gmail.com! @JamesMeadow! !
- 3. (2013) Indoor AirborneBacterial Communities Are Influenced By Ventilation, Occupancy, and Outdoor Air Source James F. Meadow Adam E. Altrichter Steven W. Kembel Jeff Kline Gwynne Mhuireach Maxwell Moriyama Dale Northcutt Timothy K. O’Connor Ann M. Womack G. Z. Brown Jessica L. Green Brendan J. M. Bohannan
- 4. Human-associated bacteria accountfor the occupied difference Meadow et al. 2013
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- 6. CAP 1 CAP2 Chairs Desks Walls Floors a CAP 1 CAP2 Lactobacillus Lactobacillus Corynebacterium Lactobacillus Staphylococcus Staphylococcus Corynebacterium Corynebacterium Phytoplasma Streptococcus Streptococcus Streptococcus Brevundimonas Sphingomonas Alicyclobacillus Alicyclobacillus Rhodopseudomonas Salmonella Chroococcidiopsis Salmonella Roseomonas b Thetype of surface predicts communities And the way we touch those surfaces predicts which bacteria are present
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- 8. JaccardSimilarity (as%ofsharedOTUs) index & own phone index & other phones 0 10 20 25% All participants index& own phone index & other phones Only women index & own phone index & other phones Only men Do you specifically resemble your own phone?!
- 11. Occupied Unoccupied Air Filter (12per treatment) Air Flow Direction Visqueen Wall 1m 0.15m Settling Dish (6 per treatment) Particle Counter (1 per treatment) 2.4 m 3.7m 2.4 m 2.9m Supply Air Plenum Test Chamber
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- 13. ● ● ● ● ● ● ● ● ● ● ● ● ●● ● ● ● ● ● ●● ● ● ● ● ● ● ●● ● ● ● ● ● ●● ● occupied unoccupied NMDS 1 NMDS2 4 Hour −60 −40 −20 0 20 40 60 OTUrichness−mean occupied unocc −0.6 −0.4 −0.2 0.0 0.2 0.4 0.6 * * SWdiversity−mean An occupied room is microbially distinct vacuum! filters!
- 14. Air filters (a) NMDS 1 NMDS2 Settlingdishes (c) NMDS 1 NMDS2 occupied unoccupied Subject 1 Subject 2 Subject 3 (b) (d) (e) 0.00 0.02 0.04 0.06 CanberraSimilarity self other self other self other All Samples Air Filters Settling Dishes Same results for airborne and settled particles
- 15. 0.00 0.01 0.02 0.03 0.04 0.05 0.06 CanberraSimilarity self other selfother self other All Samples Air Filters Settling Dishes Occupied 0.00 0.01 0.02 0.03 0.04 0.05 0.06 self other self other self other All Samples Air Filters Settling Dishes Unoccupied An occupant is microbially distinct
- 16. Occupied Unoccupied Air Filter (12per treatment) Air Flow Direction Visqueen Wall 1m 0.15m Settling Dish (6 per treatment) Particle Counter (1 per treatment) 2.4 m 3.7m 2.4 m 2.9m Supply Air Plenum Test Chamber
- 17. Air Filter (6per treatment) Air Flow Direction 1m Particle Counter (1 per treatment) 2.4 m 3.7m 2.4 m 2.9m Supply Air Plenum Test Chamber
- 18. 8 different people 2different air flow rates 1.5 hours each
- 19. Tried to keepeach person comfortable using only radiant heating 21 22 23 24 25 26 25 30 35 40 45 Rel Humidity (%) S1 S2 S3 S4 S5 S6 S7 S8 Temp (C)
- 20. 8 different occupants 1ACH 1.5 hours
- 21. 8 different occupants 1ACH 1.5 hours
- 22. 8 different occupants 1ACH 1.5 hours
- 23. 8 different occupants 1ACH 1.5 hours
- 24. 8 different occupants 3ACH 1.5 hours
- 25. 8 different occupants 3ACH 1.5 hours
- 26. Air Filter (6per treatment) Air Flow Direction 1m Particle Counter (1 per treatment) 2.4 m 3.7m 2.4 m 2.9m Supply Air Plenum Test Chamber CanberraSimilarity 0.00 0.05 0.10 0.15 inside exhaust supply self vs. self self vs. other 1 Air Change / Hour An occupant is microbially distinct … again
- 27. CanberraSimilarity 0.00 0.05 0.10 0.15 inside exhaust supply 1Air Change / Hour inside exhaust supply self vs. self self vs. other 3 ACH But air flow rate influences identifiability
- 29.