The document summarizes a study on the in vitro capacitation of ram spermatozoa. Epididymides were collected from a slaughterhouse and sperm was collected via aspiration. The sperm was incubated for 4-6 hours in media containing heparin. Mature sperm were identified by the loss of the cytoplasmic droplet and the presence of head-to-head agglutination, indicating capacitation. The results demonstrated that the procedures successfully induced sperm maturation and capacitation in vitro.
Induction of tetraploidy in an ornamental fish koicarp Cyprinus carpio L, usi...researchanimalsciences
Koicarp is potentially an important cultured ornamental fish in freshwater. Moreover there were reports existing on genetic manipulation of koicarp by application of the heat shock. Hence the present study was made to contribute a protocol for induction of tetraploidy by heat shock in the koicarp.Induction of tetraploidy was attempted in Cyprinus carpio L, Koicarp by heat shock. Eggs from five females and milt from five males ok Koicarp were pooled to ensure the required quantity and quality of gametes for fertilization. After insemination the eggs were divided into three batches each experiment based on the post fertilization viz., 25min, 27min and 30min after insemination. Batches of eggs held in plastic containers were exposed to hot water at 38° C, 39° C, 40° C & 41° C for durations of 2min and four min. One batch of the eggs without heat shock treatment was used as control. After treatments, eggs were immediately transferred to incubation troughs. Tetraploidy was ascertained by karyotyping as well as RBC nuclear micro measurements.Heat shock of 41°C for four min, imparted to eggs for 20 min after fertilization induced a maximum of 60± 2% tetraploidy and maximum hatchability of 10± 1.5%. A large proportion of the heat shocked embryos displayed morphological abnormalities such as short and curved tail, destroyed yolksac, deformed vertebral column and malformed cephalic region. A maximum of 60± 2% tetraploids (4n = 156) were obtained when the fertilized eggs (20 min old) were heat shocked at 41° C for four min duration. The tetraploid red blood cells (RBCs) nucleus volume was 2.1 times greater than those of the diploid RBC nucleus.Given that koicarp are such a useful model for other areas of research, perhaps further studies on the induction of tetraploidy in this species will lead to a better understanding of polyploidy induction and the establishment of tetraploid lines of koicarp and other species as well.
Article Citation:
Ananth Kumar and Mohamed Abdul Kadher Haniffa.
Induction of Tetraploidy in an Ornamental Fish Koicarp
Cyprinus carpio L, Using Heat Shock.
Journal of Research in Animal Sciences (2012) 1(1): 013-019.
Full Text:
http://janimalsciences.com/documents/AS0006.pdf
Induction of tetraploidy in an ornamental fish koicarp Cyprinus carpio L, usi...researchanimalsciences
Koicarp is potentially an important cultured ornamental fish in freshwater. Moreover there were reports existing on genetic manipulation of koicarp by application of the heat shock. Hence the present study was made to contribute a protocol for induction of tetraploidy by heat shock in the koicarp.Induction of tetraploidy was attempted in Cyprinus carpio L, Koicarp by heat shock. Eggs from five females and milt from five males ok Koicarp were pooled to ensure the required quantity and quality of gametes for fertilization. After insemination the eggs were divided into three batches each experiment based on the post fertilization viz., 25min, 27min and 30min after insemination. Batches of eggs held in plastic containers were exposed to hot water at 38° C, 39° C, 40° C & 41° C for durations of 2min and four min. One batch of the eggs without heat shock treatment was used as control. After treatments, eggs were immediately transferred to incubation troughs. Tetraploidy was ascertained by karyotyping as well as RBC nuclear micro measurements.Heat shock of 41°C for four min, imparted to eggs for 20 min after fertilization induced a maximum of 60± 2% tetraploidy and maximum hatchability of 10± 1.5%. A large proportion of the heat shocked embryos displayed morphological abnormalities such as short and curved tail, destroyed yolksac, deformed vertebral column and malformed cephalic region. A maximum of 60± 2% tetraploids (4n = 156) were obtained when the fertilized eggs (20 min old) were heat shocked at 41° C for four min duration. The tetraploid red blood cells (RBCs) nucleus volume was 2.1 times greater than those of the diploid RBC nucleus.Given that koicarp are such a useful model for other areas of research, perhaps further studies on the induction of tetraploidy in this species will lead to a better understanding of polyploidy induction and the establishment of tetraploid lines of koicarp and other species as well.
Article Citation:
Ananth Kumar and Mohamed Abdul Kadher Haniffa.
Induction of Tetraploidy in an Ornamental Fish Koicarp
Cyprinus carpio L, Using Heat Shock.
Journal of Research in Animal Sciences (2012) 1(1): 013-019.
Full Text:
http://janimalsciences.com/documents/AS0006.pdf
Azadirachta indica, locally called Dongo yaro is one ubiquitous native herb in Nigeria today. Though yet to be fully explored by orthodox and trado-medical practitioners, its positive and/or hazardous effect(s) remain(s) a “mysterious” to researchers, the academia, public and health care providers. In view of this, this study was instigated to investigate and chronicle (where found), the exact effect(s), that extracts from Dongo yaro (Neem Oil) will pose on the male reproductive gamete; specifically semen quality, as well as lipid peroxidation levels. To achieve this, semen from thirty (30) Normo-zoospermic males was obtained from Igbinedion University Teaching Hospital, Okada, Edo State. Specimens were then categorized under two groups (A and B) based on incubation periods per incrementally added quantities of semen and neem oil. With groups A and B respectively allotted 15 specimens (n = 15 each) for their respective subgroups (A1 through A15 and B1 through B15), test-tubes A1 through A3, and subgroup B1 (comprising of 3 specimen) were left untreated with Neem oil, thus acting as controls. photocolorimetric results show, upon comparison with other experimental groups (A4 through A3 and subgroups B2 through B5), that prolonged increase in the quantity of Dongo Yaro extract (Neem Oil) has a negative effect on male reproductive gametes, as it caused high reduction in semen quality while increasing lipid peroxidation levels as well.
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is an open access international journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Undergraduate study done in an attempt to expedite yeast growth to fit the busy biology undergraduate schedule. The yeast were growth at various time/temp/rotation increments and were monitored for growth with a spectrometer. This information is now being used by our team of graduate students on a more in depth sudy using yeast speroplasts to study apoptosis mechanisms.
this seminar includes information about various culture media and culture techniques with main focus on molecular diagnostic methods, recent advancements and studies to the process more quickly and correctly.
Azadirachta indica, locally called Dongo yaro is one ubiquitous native herb in Nigeria today. Though yet to be fully explored by orthodox and trado-medical practitioners, its positive and/or hazardous effect(s) remain(s) a “mysterious” to researchers, the academia, public and health care providers. In view of this, this study was instigated to investigate and chronicle (where found), the exact effect(s), that extracts from Dongo yaro (Neem Oil) will pose on the male reproductive gamete; specifically semen quality, as well as lipid peroxidation levels. To achieve this, semen from thirty (30) Normo-zoospermic males was obtained from Igbinedion University Teaching Hospital, Okada, Edo State. Specimens were then categorized under two groups (A and B) based on incubation periods per incrementally added quantities of semen and neem oil. With groups A and B respectively allotted 15 specimens (n = 15 each) for their respective subgroups (A1 through A15 and B1 through B15), test-tubes A1 through A3, and subgroup B1 (comprising of 3 specimen) were left untreated with Neem oil, thus acting as controls. photocolorimetric results show, upon comparison with other experimental groups (A4 through A3 and subgroups B2 through B5), that prolonged increase in the quantity of Dongo Yaro extract (Neem Oil) has a negative effect on male reproductive gametes, as it caused high reduction in semen quality while increasing lipid peroxidation levels as well.
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is an open access international journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Undergraduate study done in an attempt to expedite yeast growth to fit the busy biology undergraduate schedule. The yeast were growth at various time/temp/rotation increments and were monitored for growth with a spectrometer. This information is now being used by our team of graduate students on a more in depth sudy using yeast speroplasts to study apoptosis mechanisms.
this seminar includes information about various culture media and culture techniques with main focus on molecular diagnostic methods, recent advancements and studies to the process more quickly and correctly.
Induction of tetraploidy in an ornamental fish koicarp Cyprinus carpio L, us...researchanimalsciences
Koicarp is potentially an important cultured ornamental fish in freshwater.
Moreover there were reports existing on genetic manipulation of koicarp by
application of the heat shock. Hence the present study was made to contribute a
protocol for induction of tetraploidy by heat shock in the koicarp.Induction of
tetraploidy was attempted in
Cyprinus carpio
L, Koicarp by heat shock. Eggs from five
females and milt from five males ok Koicarp were pooled to ensure the required
quantity and quality of gametes for fertilization. After insemination the eggs were
divided into three batches each experiment based on the post fertilization viz., 25min,
27min and 30min after insemination. Batches of eggs held in plastic containers were
exposed to hot water at 38° C, 39° C, 40° C & 41° C for durations of 2min and four min.
One batch of the eggs without heat shock treatment was used as control. After
treatments, eggs were immediately transferred to incubation troughs. Tetraploidy
was ascertained by karyotyping as well as RBC nuclear micro measurements.Heat
shock of 41°C for four min, imparted to eggs for 20 min after fertilization induced a
maximum of 60± 2% tetraploidy and maximum hatchability of 10± 1.5%. A large
proportion of the heat shocked embryos displayed morphological abnormalities such
as short and curved tail, destroyed yolksac, deformed vertebral column and
malformed cephalic region. A maximum of 60± 2% tetraploids (4n = 156) were
obtained when the fertilized eggs (20 min old) were heat shocked at 41° C for four
min duration. The tetraploid red blood cells (RBCs) nucleus volume was 2.1 times
greater than those of the diploid RBC nucleus.Given that koicarp are such a useful
model for other areas of research, perhaps further studies on the induction of
tetraploidy in this species will lead to a better understanding of polyploidy induction
and the establishment of tetraploid lines of koicarp and other species as well.
Preliminary evaluation of the larvicidal efficacy of coelomic fluid of Eudril...inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Progesterone Profile of Mature Nubian Goats iosrjce
Two experiments were carried out to record the progesterone (P4) profile of Nubian goats during
oestrous cycle and postpartum. In experiment I, 8 cyclic does were i.m injected with 2 doses of 125µg of
prostaglandin F2α (PG F2α) 11 days apart to synchronize oestrous. Eleven serum samples, were collected from
each doe at an interval of 2 days after the commencement of behavioral oestrous sings (day 0) and assayed for
P4 levels. The mean P4 concentration (conc.) on day 0 was 0.12 ± 0.01 ng/ml, then it increased gradually to
reach a peak of 6.03 ± 0.25 ng/ml on day 10 and it assumed a plateau over days 12 to 16. A sharp decline in P4
conc. was recorded on day 18 (0.3 ± 0.01 ng/ml) and a further drop (0.19 ± 0.00 ng/ml) ended the cycle on day
20. In experiment II, 10 parturient does were employed to study P4 profile during postpartum. Twenty one milk
samples, collected from each doe at 4 days interval starting from day 3 postpartum, were assayed for P4 levels.
The milk P 4 level remained below 0.04 ng/ml until a mean of 45 days postpartum; thereafter it increased to
attain values ≥ 1.0 ng/ml after the commencement of the first oestrus postpartum. It is concluded that P4 profile
of the Sudan Nubian goats during oestrous cycle and postpartum follows the normal trend of the P4 profile of
other breeds of goats with very minute differences in P4 conc. and the timing of peak values.
Effect of lyophilized aqueous leaf extract of Aquilaria subintegra on aphrodi...Mohammed Muayad TA
Objective: To investigate the effects of Aquilaria subintegra leaf aqueous extracts on the
aphrodisiac properties including sexual behaviour, testosterone level, percentage of pregnancy,
number of offspring and male to female ratio of offspring in ICR mice.
Methods: In this experiment, each male cohabitated with one female in a polysulfone cage.
30 ICR male mice were divided into 6 groups that received normal saline (the control group),
50 mg/kg, 100 mg/kg, 200 mg/kg, 500 mg/kg, and 1 000 mg/kg body weight of Aquilaria
subintegra leaf aqueous extracts orally for 21 days consecutively. Sexual behavior, percentage
of pregnancy, number of offspring and male to female ratio of offspring in ICR mice were
measured according to the established methods. Testosterone level was measured by using
enzyme-linked immunosorbent assay.
Results: Mice that received Aquilaria subintegra leaf aqueous extracts at 50 mg/kg body
weight (day 0) had significantly higher mount frequency as compared to the control group;
groups treated with 100, 500, 1 000 mg/kg body weight extracts produced a greater number
of offsprings when compared to the control group. All aphrodisiac parameters were similar
between the treatment groups and the control group, indicating that Aquilaria subintegra leaf
aqueous extract did not significantly alter the aphrodisiac parameters.
Conclusions: Aquilaria subintegra leaf aqueous extracts have no effect on the aphrodisiac
properties, but could increase the breeding rate in mice.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Phenomics assisted breeding in crop improvementIshaGoswami9
As the population is increasing and will reach about 9 billion upto 2050. Also due to climate change, it is difficult to meet the food requirement of such a large population. Facing the challenges presented by resource shortages, climate
change, and increasing global population, crop yield and quality need to be improved in a sustainable way over the coming decades. Genetic improvement by breeding is the best way to increase crop productivity. With the rapid progression of functional
genomics, an increasing number of crop genomes have been sequenced and dozens of genes influencing key agronomic traits have been identified. However, current genome sequence information has not been adequately exploited for understanding
the complex characteristics of multiple gene, owing to a lack of crop phenotypic data. Efficient, automatic, and accurate technologies and platforms that can capture phenotypic data that can
be linked to genomics information for crop improvement at all growth stages have become as important as genotyping. Thus,
high-throughput phenotyping has become the major bottleneck restricting crop breeding. Plant phenomics has been defined as the high-throughput, accurate acquisition and analysis of multi-dimensional phenotypes
during crop growing stages at the organism level, including the cell, tissue, organ, individual plant, plot, and field levels. With the rapid development of novel sensors, imaging technology,
and analysis methods, numerous infrastructure platforms have been developed for phenotyping.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Remote Sensing and Computational, Evolutionary, Supercomputing, and Intellige...University of Maribor
Slides from talk:
Aleš Zamuda: Remote Sensing and Computational, Evolutionary, Supercomputing, and Intelligent Systems.
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Inter-Society Networking Panel GRSS/MTT-S/CIS Panel Session: Promoting Connection and Cooperation
https://www.etran.rs/2024/en/home-english/
1. Al-Anbar J. Vet. Sci., Vol.: 4, Supplement, 2011 ISSN: 1999-6527
Proceedings of First Medical Conference of Medical Colleges (Veterinary Research)
University of Anbar
94
In Vitro Capacitation of Ram Spermatozoa
A. F. Majeed* and M. M. T. Al-Jumaily**
*College of Veterinary Medicine University of Anbar
**College of Agriculture University of Anbar
Summary
Epididymus were collected from Al-Ramadi Slaughter house during the periods
from September 2002 to march 2003. The samples were transported to the Laboratory
with normal saline at a temp of 33-35c within 30 minutes. Semen were collected from
the tail of epididymus by aspiration with 18 gauge needle on 3ml disposable suring
containing 1-2 ml TALP media. The collected media together with spermatozoa were
put in a Petri dish and incubated in Co2 incubator at 35 c, 90% Humidity for 4-6 h.
Spermatozoa evaluated for their maturation (The distal Protoplasmic droplet). Matured
Spermatozoa were washed with buffer Solution. Three times with Centrifugation. Then
0.5 ml of spermatozoa pluse 9.5 ml of TALP media with 10 mg/ml heparin,
Centrifuged, and then 0.5 ml from the bottom spermatozoa incubated in Co2 incubator
at 35c with 45 angle. Head to Head agglutination of Spermatozoa was considered the
Criteriia of sperm Capacitation. The results showed that the procedures give a best
result for sperm maturation and capacitation in vitro. It was concluded that a possibility
of sperm maturation and capacition taken from cauda epididymus of ram from slaughter
house sources.
ﻣﺧﺗﺑرﯾﺎ اﻟﻛﺑش ﺣﯾﺎﻣن ﺗﻛﯾﯾف
ﻣﺟﯾد ﻓرج اﻟﺳﺗﺎر ﻋﺑد*اﻟﺟﻣﯾﻠﻲ طﻪ ﻣؤﯾد وﻣﺣﻣد**
*ياﻟﺑﯾطر اﻟطب ﻛﻠﯾﺔ/اﻷﻧﺑﺎر ﺟﺎﻣﻌﺔ
**اﻋﺔراﻟز ﻛﻠﯾﺔ/اﻷﻧﺑﺎر ﺟﺎﻣﻌﺔ
اﻟﺧﻼﺻﺔ
ط ﻋن اﺳﺔراﻟد ﯾترأﺟاﻟرﻣﺎدي ةرﻣﺟز ﻣن ﺑﺦراﻟﺑ ﺟﻣﻊ ﯾقر،ـولﻠأﯾ ﻣن ةراﻟﻔﺗ ﺧﻼل2002ـﺎﯾسﻣ ـﺔﯾﻟﻐﺎ2003.
ــﺳاوﺑ ـﺎتـﻧاﻟﻌﯾ ـتـﻠﻧﻘةرارــﺣ ـﺔـﺟوﺑدر ـﻠﺟﻲـﺳاﻟﻔ ـﺢـﻠاﻟﻣ ـولـﻠﻣﺣ ـﻰـﻠﻋ ـﺔـﯾﺣﺎو ـردةـﺑﻣ ـﺔـﯾﺣﺎو طﺔ33-35ْﻰــﻟإـﻲـﻓ ـرـﺑاﻟﻣﺧﺗ
ﺧﻼل اﻋﺔراﻟز ﻛﻠﯾﺔ30دﻗﯾﺔﻘ.ـذﻩﯾﻧﺑ ﻧﺞرـﺳﺑ ـﺣبﺳاﻟ اﺳطﺔوﺑ ﺑﺦراﻟﺑ ذﯾل ﻣن اﻟﻣﻧوي اﻟﺳﺎﺋل ﺳﺣب3ـﻰﻠﻋ ـﺔﯾﺣﺎو ـلﻣ
1-2ـﻲــﻋاﻟزر ـطــﺳاﻟو ـنــﻣ ـلــﻣ)TALP(.اﻟ يـرــﺗﺑ ـﺎقــﺑأط ـﻲــﻓ ـﻲــﻋاﻟزر ـطــﺳاﻟوو ـﺎﻣنــﯾاﻟﺣ ـﻌتــﺿوـتــﺿوﺧ ـﺔــﻣﻣﻌﻘ
ـﻧﺔـﺿﺣﺎ ـﺗﺧدامـﺳﺑﺎﺑونرـﺎـﻛاﻟ ـﯾدـﺳاوﻛ ـﺎﻧﻲـﺛ5%،ـﺔـﺑورطو90%ةرارــﺣ ـﺔـﺟوﺑدر35ْـدةـﻣﻟ4-6ـﺎﻋﺎتـﺳـﺎجـﺿﻹﻧ
اﻟﺣﯾﺎﻣن.ﺟﺔدر ﻗﯾﻣت وﻗدإﻧﺿﺎﺟﻬﺎـﻔﻠﻰﺳاﻟ اﻟﻬﯾوﻟﺔ ةراﻟﻘط ﻣوﻗﻊ ﻋﻠﻰ اﻋﺗﻣﺎدا.ـﻊﻣ ـﻠتﺳوﻏ ـﺟﺔﺿاﻟﻧﺎ ـﺎﻣنﯾاﻟﺣ ـذتﺧأ
اترــﻣ ـﺛﻼثﻟو يـدارـﻟا ـلﺳاﻟﻐ ـولﻠﻣﺣاﻟﻣ ـردطاﻟ ـﻊـﻣ ـﺔﯾﻣﺗﺗﺎﻟيزـﻛر.ـﺎرﺑاﻻﺧﺗ ـﺔـﺑأﻧﺑو ـرﻌﻗ ـنـﻣ ـﺎﻣنﯾاﻟﺣ ـذتـﺧأ0.5ـلـﻣ
أﺿﯾفوإﻟﯾﻬﺎﻋﻲاﻟزر اﻟوﺳط9.5ﻣﻊ ﻣلإﺿﺎﻓﺔ10امرﻣﺎﯾﻛروﻏ/ﯾنرـﺎﺑﻫﯾ ـلﻣ.وـنﺿﺣـدةﻣﻟ15ـﺔﯾاوزوﺑ ـﺔﻘدﻗﯾ45
ﺟﺔدرـﻧﺔﺿﺣﺎ ـﻲﻓ ـﺎرﺑاﻻﺧﺗ أﻧﺑوﺑﺔ ﻓﻲCO25%،90%ةرارـﺣ ـﺔﺑرطو35ـﺎرﯾاﻟﻣﻌ ـﺎﻣنﯾﻟﻠﺣ ـﻲﺳأراﻟ ـﺗﻼزنﻟا ـرﺑأﻋﺗ م
ﻟﺣدوث"اﻟﺳﻌﺔ"Capacitationـﺎﻣنﯾﻟﻠﺣ.ـﻌﺗﻬﺎﺳو ـﺎﻣنﯾاﻟﺣ ـﺎجﺿإﻧ ـﻲﻓ ـﺗﺧدﻣﺔﺳاﻟﻣ ـﺔﻘﯾراﻟط أن ـﺎﺋﺞﺗاﻟﻧ ـرتﻬأظ ـدﻗو
اﻟﻣﺧﺗﺑر ﻓﻲ اﻟﺗﺣﺿر ﺳﻬﻠﺔ.ـﺎبﺻاﻻﺧ ـﻲﻓ ـﻌﺗﻬﺎﺳو ـﺎﺟﻬﺎﺿإﻧ ـدﻌﺑ ﺑﺦرـﺑاﻟ ـلﯾذ ـﺎﻣنﯾﺣ ـﺗﺧدامﺳأ ـﺎنﻛﺑﺎﻻﻣ ـﺗﻧﺗﺞﺳأ ـدﻗو
ﺟﻲاﻟﺧﺎر.
2. Al-Anbar J. Vet. Sci., Vol.: 4, Supplement, 2011 ISSN: 1999-6527
Proceedings of First Medical Conference of Medical Colleges (Veterinary Research)
University of Anbar
95
Introduction
One of the important requirements for successful in vitro fertilization was the sperm
capacitation. Capacitation defined as a series of biochemical and biophysical changes
prior to fertilization (1). Capacitation involves the removal of sperm coating material
acquired during epididymal transit or during exposure to seminal plasma and cholesterol
depletion resulting in increased membrane permeability to calcium (2). In vitro washing
of spermatozoa before incubation was reported to accelerate capacitation. Heparin is
used for in vitro capacitation of spermatozoa. It modulates gamete interactions in cattle
and sheep IVE system (3). The aim of this study was designed to show the capcitation
of arm spermatozoa obtained from the tail of epididymis.
Materials and Methods
Epididymus were collected from Al-Ramadi Abattoir during the periods from
September 2002 to march 2003. The samples were transported to lab with normal saline
with temp 33-35c within 30 min, semen were collected from the tail of epididymus by
aspiration with 3 ml disposable suring Containing 1-2 ml of TALP media according to
the size of the tail of epididymus according to Im, etal (4). Then the tail of epididymis
minced with medical scalpel in order to carryout the sperm in the media. The collected
media together with the sperms were pult in a sterile petridish and then transported to be
incubated at 35 c. Samples were taken from the media to observe the degree of
maturation and evaluate the sperm according to the presence of protoplasm droplet and
their position on the sperm. Evaluation of semen included individual motility and sperm
abnormalities. The sperms then were incubated at 5% Co2 ,35 c, 90% relative humidity
for 4-6 hours. In a sterile test tubes with 45 angle without cover.
- In vitro Capacitation of Sperms: Following in vitro maturation and examined
under microscope to see the degree of sperm maturation, Then the sperms leaved in
the media outside the incubator at room temp (23-26c) for 30 minutes. After that the
sperms were washed with buffer solution (at temp 23-25c) [nacl; 9 mg/ ml, BSA; 1
mg/ ml, FCS; 10%, Cryst. Penicillin; 100 I.U./ ML, Streptomycin. Sulphate; 0.1
mg/ml]. 1 ml from semen diluted with washed solution to 10 ml. in a test tubes, then
centrifuged at room temp 1200 R. per. minte/ for 3 minutes for three successive
times. Discard the supernatant fluid and the sperms stays at the bottoms of the test
tubes with a little amounts of media. Addition of 10 ml of TALP media [NACL;
114.0mm, KCL; 3.2mm, Nahco3; 25.0mm, NaH2Po4; 0.3mm, Sodium Lactate;
21.0mm, Cacl2; 2.0mm, Mgcl2; 0.5mm, Sodium Pyruvate; 1.0 mm, Heparin; 10
mg/ml, BSA; mg /ml, Streptomycin Sulphate; 50 mg/ml, Crystalline Penicillin; 100
i.u./ml]. Then Centrifuged again at the same previous manner for one time. 9 ml of
supernatant of the media was removed, Incubated in Co2 incubator for 15 minutes at
35c with 45 angle Head to head agglutination of spermatozoa is considered to be a
Criteria of sperm capacitation(1).
Results and Discussion
The results of this study showed the possibility of sperms maturation taken from the
tail of epididymis in TALP media in vitro. The presence of distal protoplasmic droplet
was the criteria of sperm maturation. Similar observations have been made by Wani (5),
Wani, etal. (6) and Al-Jumaily (7). Several workers indicated that epididymal
spermatozoa have been successfully used from slaughtered rams, many hours after their
death, for IVF of sheep oocytes (1, 5, 7). TALP cultured media for in vitro maturation
of sperms were easily prepared media in the Lab (8). This might be attributed to their
3. Al-Anbar J. Vet. Sci., Vol.: 4, Supplement, 2011 ISSN: 1999-6527
Proceedings of First Medical Conference of Medical Colleges (Veterinary Research)
University of Anbar
96
content of chemical material. The result also showed the criteria of head to head
agglutination, which indicates the sperm capacitation. It has been observed that heparin
stimulates the fertilization rate by improving the efficiency of sperm capacitation in
sheep (9). Heparin apparently binds to sperm and plays a role in the sperm uptake of
calcium (10). Capacitation involves the alternation of the plasma membrane, such as
removal of decapicitation factors, removal of cholesterol, influx of calcium, an increase
in intracellular PH, and increase in protein tyrosine phosphorylation. The Majority of
changes that occur during capacitation involve sperm head; however no morphological
occur. Capacitation is believed to allow the sperm to undergo the a croseme reaction.
The life span of capacitation sperm is limited; as a result, precise timing between
capacitation and the acrosome reaction is necessary for proper fertilization to occur (3).
References
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