This report describes an immunofluorescence analysis of an anti-Kif 7 monoclonal antibody (clone 3F8) on mouse colon tissue. The tissue was processed using antigen retrieval and blocking steps. The primary antibody was incubated overnight at a 1:200 dilution, followed by a Cy3-labeled secondary antibody for 50 minutes. DAPI counterstaining was also performed. Images were captured using a fluorescence microscope to visualize the target, DAPI, and merged signals.