This report describes an immunofluorescence analysis of an anti-MICU1 monoclonal antibody on human appendix tissue. The tissue was processed using antigen retrieval, blocking, and staining with the primary and secondary antibodies overnight and 50 minutes respectively. It was then counterstained with DAPI and mounted for visualization under a fluorescence microscope. The report provides details on the antibody, tissue, staining protocol, and microscope used for analysis.