This antibody validation report from St. John's Laboratory describes the testing of an anti-Nrf2 polyclonal antibody in human stomach and rat lung tissues using immunofluorescence. The report details the immunofluorescence protocol including tissue processing, antigen retrieval, blocking, primary and secondary antibody incubations, DAPI counterstaining, mounting and visualization under a fluorescence microscope. Images show specific nuclear staining of Nrf2 in both tissues when incubated with the anti-Nrf2 antibody.