This document discusses restriction fragment length polymorphism (RFLP) analysis, which is a technique used to detect genetic variations. It involves cutting DNA with restriction enzymes, separating the fragments via gel electrophoresis, and probing the gel to visualize differences in fragment lengths between individuals. The document provides details on restriction enzymes, how they recognize and cut DNA sequences, and how RFLP analysis is performed as a tool for genome mapping, disease gene localization, and other applications. It also includes an example of RFLP analysis to identify a mutation in the NPHS2 gene associated with nephrotic syndrome.