The study evaluates the performance of the FUS-2000 urinalysis hybrid against conventional microscopic examination using 334 urine samples. Results indicate a high correlation between the two methods for leukocytes, erythrocytes, and squamous epithelial cells, though significant differences were observed, while no difference was found for bacteria. The FUS-2000 shows promise for routine laboratory use but requires calibration for accurate grading.

1. Comparison and Evaluation of FUS-2000
Urinalysis Hybrid and Conventional
Microscopic Examination
Prangthip Sukboon1, Supichcha Duangson1, Kridsada Sirisabhabhorn2,Chollanot Kaset1, Supaporn Pumpa2, Daungnate Pipatsatitpong1*
1Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University
2Division of Clinical Microscopy and Parasitology, Department of Medical Technology Laboratory, Thammasat University Hospital
To evaluate performances of the FUS-2000 Urinalysis Hybrid compared with
manual microscopic examination.
Three hundred and thirty four urine samples were collected from submitted
inpatients for diagnostic urinalysis to the laboratory at Thammasat University
hospital. All urine samples were examined by FUS-2000 and manual microscopy.
Statistical analysis; leukocyte, erythrocyte, squamous epithelial cells and bacteria
grading were analyzed by Wilcoxon signed ranks test and Spearman correlation.
334 Urine
sample were
collected
Examined by
FUS-2000
Urinalysis Hybrid
Centrifuged at
1500 rpm for 5
minutes
Manual
microscopic
examination
Data was analyzed
by Wilcoxon signed
ranks test and
Spearman
correlation
Parameters
Spearman correlation of coefficient (r)
FUS-2000 Urinalysis Hybrid
Manual
Leukocyte 0.887*
Erythrocyte 0.790*
Epithelial cells 0.709*
Bacteria 0.757*
The Spearman’s statistic showed the high correlation between FUS-2000
Urinalysis Hybrid and manual microscopy for leukocyte (r=0.887) ,
erythrocyte (r=0.790) , squamous epithelial cells (r=0.709)
and bacteria (r=0.757).
Parameters
p-value
FUS-2000 Urinalysis Hybrid
Manual
Leukocyte <0.001*
Erythrocyte <0.001*
Epithelial cells <0.001*
Bacteria 0.631
The Wilcoxon signed ranks test showed that the result from FUS-2000 and
manual microscopy was statistic significantly different for leukocyte
(p<0.001), erythrocyte (p<0.001), and squamous epithelial-cells (p<0.001).
But for the bacteria, the result was not different (p=0.631).
Automated urinalysis becomes more
essential in the laboratory for reducing
human errors and turn-around time. The
FUS-2000 Urinalysis Hybrid (Dirui Industrial
Co. Ltd., China) is the new urine analyzer
that has been introduced recently.
This analyzer uses digital image based
automatic particle recognition system.
INTRODUCTION
OBJECTIVE
MATERIALS AND METHODS
RESULTS
The results showed that there were a high correlation
between the FUS-2000 Urinalysis Hybrid and Manual microscopic
examination. Even though the grading number of leukocyte,
erythrocyte and squamous epithelial cells were significantly
different (one level grading), the grading number of bacteria was
not different. It can be concluded the FUS-2000 can be used in
routine laboratories but it still needs to be calibrated in order to get
as accurate grading result.
The FUS-2000 showed good performances. It was time-
saving and had standardized techniques for reducing pre-analytical
errors such as specimen volume for analysis, centrifugation.
DISCUSSION & CONCLUSION
1. Yüksel H, Evliyaoǧlu O, Kiliç E, Ekinci A. Comparison of fully automated urine sediment
analyzers H800-FUS100 and LabUMat-UriSed with manual microscopy. J Clin Lab Anal. 2013;
27(4): 312-6.
2. Mayo S, Acevedo D, Quiñones-Torrelo C, Canós I, Sancho M. Clinical laboratory
automated urinalysis: comparison among automated microscopy, flow cytometry, two test
strips analyzers, and manual microscopic examination of the urine sediments. J Clin Lab
Anal. 2008; 22(4): 262-70.
REFERENCES
ACKNOWLEDGEMENTS
We are grateful to thank Mr.Palakorn Puttarak,
The Laboratory Director of Medical Technology Laboratory,
Thammasat University Hospital, who provided us with work space
and equipments.
We also sincerely thank RND Diagnostics Co.Ltd (under
MP Group) for supporting reagents and equipments.
The FUS-2000 uses uncentrifuged urine
for digital imaging to capture and analyze 650
photos from each sample for an artificial
intelligence identification technology in order
to auto-classify the isolated images of the urine
sediment constituents, and report them
quantitatively.
*p< 0.05
*p <0.05
Table 1 : p-value of FUS-2000 vs Manual microscopic examination analyzed by Wilcoxson test
Table 2 : The Spearman’s correlation coefficient of FUS-2000 vs Manual microscopic examination
*Address correspondence: Duangnate Pipatsatitpong, Faculty of Allied Health Sciences,
Thammasat University, Thailand.
E-mail: duangnate@tu.ac.th , duangnate_pipat@hotmail.com