This document summarizes heavy metal toxicity in animals. It discusses various heavy metal contaminations across India including from lead in water, arsenic contamination of groundwater in West Bengal and Bangladesh, and various industrial sites contaminated with heavy metals. It also outlines the clinical signs and target organ toxicity of several heavy metals including lead, mercury, cadmium, and arsenic as well as methods for managing heavy metal toxicity.
Toxicity is a function of solubility. Insoluble compounds as well as the metallic forms often exhibit negligible toxicity. The toxicity of any metal depends on its ligands. Heavy metal toxicity can result in damaged or reduced mental and central nervous function, lower energy levels, and damage to blood composition, lungs, kidneys, liver, and other vital organs.
Toxicity is a function of solubility. Insoluble compounds as well as the metallic forms often exhibit negligible toxicity. The toxicity of any metal depends on its ligands. Heavy metal toxicity can result in damaged or reduced mental and central nervous function, lower energy levels, and damage to blood composition, lungs, kidneys, liver, and other vital organs.
INTRODUCTION
Toxicology is the science of the poisons. It also studies the nature, effects, detection, assessment and treatment of their effects on biological material.
Toxicology is a multidisciplinary science. The ultimate objective of the combined research is to determine how an organism is affected by exposure to an agent.
This includes an understanding of:
How the agent moves and interact with living cells and tissues of the organism;
What parts of the organism are affected by its presence and health outcomes of this exposure.
Evaluation of the toxicity of substances whose biological effects may not have been well characterized.
The influence of chemical toxicity is mainly
determined by the dosage, duration of exposure,
route of exposure, species, age, sex, and environment.
The goal of toxicology is to contribute to the
general knowledge and harmful actions of
chemical substances.
2. to study their mechanisms of action,
3. and to estimate their possible risks to humans
HISTORY
Dioscorides, a Greek physician in the court of the Roman emperor Nero, made the first attempt to classify plants according to their toxic and therapeutic effect. Poisonous plants and animals were recognized and their extracts used for hunting or in warfare.
In 1500 BC people used hemlock, opium, arrow poisons, and certain metals to poison enemies or for state executions.
Theophrastus Phillipus Auroleus Bombastus von Hohenheim (1493–1541) (also referred to as Paracelsus, a Roman physician from the first century) is considered "the father" of toxicology.
He stated that "All things are poisonous and nothing is without poison; only the dose makes a thing not poisonous.“
Mathieu Orfila (1813) is considered the modern father of toxicology.
In 1850, Jean Stas became the first person to successfully isolate plant poisons from human tissue.
Hippolyte Visart de Bocarmé used nicotine to kill his brother-in-law. He extracted nicotine from tobacco leaves.
The 20th and 21st Centuries have marked by great advancements in the level of understanding of toxicology. DNA and various biochemicals that maintain body functions have been discovered. Our level of knowledge of toxic effects on organs and cells has expanded to the molecular level.
mineral toxicity is common in animals while ingest higher amount of minerals than recommended level. here there is some level of toxicity, symptoms and there ameliorative measure.
Brief ideas about the heavy metals and their poisoning. Actual reasons behind their pollution and contamination. Which type of disease occurred by their exposure. Real scenario of the Bangladesh by the contamination and pollution of heavy metals through their exposure
This is just to give the information about Eco-Tourism that what is all about with one of the examples of Keoladeo National Park. This is only a starting after this you will able to find more presentations regarding the tourism & Education.
INTRODUCTION
Toxicology is the science of the poisons. It also studies the nature, effects, detection, assessment and treatment of their effects on biological material.
Toxicology is a multidisciplinary science. The ultimate objective of the combined research is to determine how an organism is affected by exposure to an agent.
This includes an understanding of:
How the agent moves and interact with living cells and tissues of the organism;
What parts of the organism are affected by its presence and health outcomes of this exposure.
Evaluation of the toxicity of substances whose biological effects may not have been well characterized.
The influence of chemical toxicity is mainly
determined by the dosage, duration of exposure,
route of exposure, species, age, sex, and environment.
The goal of toxicology is to contribute to the
general knowledge and harmful actions of
chemical substances.
2. to study their mechanisms of action,
3. and to estimate their possible risks to humans
HISTORY
Dioscorides, a Greek physician in the court of the Roman emperor Nero, made the first attempt to classify plants according to their toxic and therapeutic effect. Poisonous plants and animals were recognized and their extracts used for hunting or in warfare.
In 1500 BC people used hemlock, opium, arrow poisons, and certain metals to poison enemies or for state executions.
Theophrastus Phillipus Auroleus Bombastus von Hohenheim (1493–1541) (also referred to as Paracelsus, a Roman physician from the first century) is considered "the father" of toxicology.
He stated that "All things are poisonous and nothing is without poison; only the dose makes a thing not poisonous.“
Mathieu Orfila (1813) is considered the modern father of toxicology.
In 1850, Jean Stas became the first person to successfully isolate plant poisons from human tissue.
Hippolyte Visart de Bocarmé used nicotine to kill his brother-in-law. He extracted nicotine from tobacco leaves.
The 20th and 21st Centuries have marked by great advancements in the level of understanding of toxicology. DNA and various biochemicals that maintain body functions have been discovered. Our level of knowledge of toxic effects on organs and cells has expanded to the molecular level.
mineral toxicity is common in animals while ingest higher amount of minerals than recommended level. here there is some level of toxicity, symptoms and there ameliorative measure.
Brief ideas about the heavy metals and their poisoning. Actual reasons behind their pollution and contamination. Which type of disease occurred by their exposure. Real scenario of the Bangladesh by the contamination and pollution of heavy metals through their exposure
This is just to give the information about Eco-Tourism that what is all about with one of the examples of Keoladeo National Park. This is only a starting after this you will able to find more presentations regarding the tourism & Education.
Tourism involving travel to areas of natural or ecological interest, for the purpose of observing wildlife and learning about the environment.
Eco-tourism is consecrated for preserving and sustaining the diversity of the world's natural and cultural environments. It accommodates and entertains visitors in a way that is minimally intrusive or destructive to the environment and sustains & supports the native cultures in the locations it is operating in. Responsibility of both travellers and service providers is the genuine meaning for eco-tourism.
Isolation and Characterization of Nickel Tolerant Bacterial Strains from Elec...Agriculture Journal IJOEAR
Abstract— In the present study, an attempt was made to isolate and characterize nickel tolerant bacterial strains from the electroplating effluent contaminated soil. The effluent sample was collected at the direct outlet of electroplating industry and analyzed for physico-chemical characteristics such as pH (6.5), temperature (33), electrical conductivity (15.1 ms/cm), total solids (2309mg/l), total dissolved solids (5573 mg/l), chloride (0.20mg/l), sodium (0.13ppm), calcium (2.23ppm), potassium (0.20ppm), Biological Oxygen Demand (4200mg/l), Chemical Oxygen Demand (5243 mg/l) and nickel (4.063ppm). Enumeration of total bacterial population from the electroplating effluent contaminated soil sample was made in nutrient agar medium. Sixteen bacterial colonies were selected based on their abundance growth all of them were identified through morphological and biochemical characteristics. All the sixteen bacterial isolates were screened for its metal tolerance using nutrient agar medium incorporated with nickel metal. Based on the better growth performance, six bacterial strains were selected as potential metal tolerant organism. The selected metal tolerant bacterial strains were further characterized in the various environmental conditions such as pH (5, 7 & 9) temperature (5°C, 28°C, 37°C & 45°C) and concentration of metal ions (100ppm, 200ppm, 300ppm & 400ppm) for 5 days. The result reveals that one bacterial strain, Pseudomonas sp 1 was showed better growth in nickel metal based medium with pH 7 at 37°C temperature.
Research Inventy : International Journal of Engineering and Scienceresearchinventy
Research Inventy : International Journal of Engineering and Science is published by the group of young academic and industrial researchers with 12 Issues per year. It is an online as well as print version open access journal that provides rapid publication (monthly) of articles in all areas of the subject such as: civil, mechanical, chemical, electronic and computer engineering as well as production and information technology. The Journal welcomes the submission of manuscripts that meet the general criteria of significance and scientific excellence. Papers will be published by rapid process within 20 days after acceptance and peer review process takes only 7 days. All articles published in Research Inventy will be peer-reviewed.
Common Heavy Metal Contaminations and Its Hazard Sotheara Kong
This piece of education slide provide you a general information about 4 Common Heavy Metal Contamination and its Hazard in ground water. It consist of many information that may has advantage to the researcher especially someone who researching on removable of chemical contamination from ground water.
Heavy Metals Contamination Levels In Suya Meat Marketed In Selected Towns In ...iosrjce
IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT) multidisciplinary peer-reviewed Journal with reputable academics and experts as board member. IOSR-JESTFT is designed for the prompt publication of peer-reviewed articles in all areas of subject. The journal articles will be accessed freely online.
The Use Of Pistia stratiotes To Remove Some Heavy Metals From Romi Stream: A ...iosrjce
The study involved a laboratory experiment on the use of Pistia stratiotes in the removal of some
heavy metals from a stream polluted by waste water from Kaduna Refinery and Petrochemical Company. Water
sample was collected from Kaduna Refinery effluent point, Romi up and Romi down. The Bioconcentration
(BCF) and Biotranslocation (BTF) Factors of each metal were determined. The experinmental study showed
that Pistia stratiotes is a suitable candidate for effective removal of heavy metals (Hg, Cd, Mn, Ag, Pb, Zn) from
Romi stream.
Physico-Chemical Evaluation of Wastewater from Abattoir, Brewery, Soap and Oi...IJERA Editor
The discharge of industrial wastewater in the city of Moundou deteriorates the quality of surface and
underground water and soils. In this study the physicochemical quality of industrial effluents was investigated in
different seasons (summer, winter and rainy). Three sampling sites were used (Central Abattoir discharge,
Cotontchad (soap and oil factory) discharge, and Brewery discharge), for sampling from July 2013 to December
2014. The following physico-chemical parameters were determined: pH, Temperature, EC, dissolved oxygen,
COD, BOD5, NO3,PO4,SO4. Also, the heavy metals: Cu, Cd, Mn, Ni, Pb, As, Zn, Cr, Fe, Al, was analyzed on
spectrophotometers and results were compared with World Health Organization (WHO) permissible limits.
This study revealed that most parameters were much higher than the permissible limit for wastewater
discharges:some parameters were to higher: pH (12,6), Temperature (37,8 °C), C.E (4270 μS/cm), organic
matters: COD (1200 mg/l), SO4 (1280 mg/l), PO4(4460 mg/l), NO3 (63,6 mg/l), (Fe (63,34 mg/l), Zn (13,27
mg/l), Pb (4,0 mg/l), Cu (25,34 mg/l), Cd (31,78 mg/l), Cr (5,9 mg/l), Ni (39,5 mg/l. The study concludes that
discharge of effluents by the companies; factory and materials from other anthropogenic sources severely
pollute the Logone River with heavy metals and other pollutants. We recommended that each industry recycle
its wastewater and put in place specific treatment plants, because pollutants to eliminate vary depending on the
industry.
Physico-Chemical Evaluation of Wastewater from Abattoir, Brewery, Soap and Oi...IJERA Editor
The discharge of industrial wastewater in the city of Moundou deteriorates the quality of surface and
underground water and soils. In this study the physicochemical quality of industrial effluents was investigated in
different seasons (summer, winter and rainy). Three sampling sites were used (Central Abattoir discharge,
Cotontchad (soap and oil factory) discharge, and Brewery discharge), for sampling from July 2013 to December
2014. The following physico-chemical parameters were determined: pH, Temperature, EC, dissolved oxygen,
COD, BOD5, NO3,PO4,SO4. Also, the heavy metals: Cu, Cd, Mn, Ni, Pb, As, Zn, Cr, Fe, Al, was analyzed on
spectrophotometers and results were compared with World Health Organization (WHO) permissible limits.
This study revealed that most parameters were much higher than the permissible limit for wastewater
discharges:some parameters were to higher: pH (12,6), Temperature (37,8 °C), C.E (4270 µS/cm), organic
matters: COD (1200 mg/l), SO4 (1280 mg/l), PO4(4460 mg/l), NO3 (63,6 mg/l), (Fe (63,34 mg/l), Zn (13,27
mg/l), Pb (4,0 mg/l), Cu (25,34 mg/l), Cd (31,78 mg/l), Cr (5,9 mg/l), Ni (39,5 mg/l. The study concludes that
discharge of effluents by the companies; factory and materials from other anthropogenic sources severely
pollute the Logone River with heavy metals and other pollutants. We recommended that each industry recycle
its wastewater and put in place specific treatment plants, because pollutants to eliminate vary depending on the
industry.
The Use Of Ecchornia crassipes To Remove Some Heavy Metals From Romi Stream: ...iosrjce
The study involved a laboratory experiment on the use of Ecchornia crassipes in the removal of
some heavy metals from a stream polluted by waste water from Kaduna Refinery and Petrochemical Company.
Water sample was collected from Kaduna Refinery effluent point, Romi up and Romi down. The
Bioconcentration (BCF) and Biotranslocation (BTF) Factors of each metal were determined. The experinmental
study showed that Ecchornia crassipes is a suitable candidate for effective removal of heavy metals (Hg, Cd,
Mn, Ag, Pb, Zn) from Romi stream.
Phytoremediation is a low cost and effective soil
treatment option for metal reclamation. The use of plants to
remove heavy metals from soil is the phytoremediation. Heavy
metals are among the most dangerous substances in the
environment because of their high level of persistence and
harmfulness to living organisms. The present study in the field
deals with phytoremediation of heavy metals from contaminated
soil around Steel industry at Boisar Industrial area, using Indian
mustard (Brassica juncea L.) plant. The impact of addition of
chelating agents like EDTA (Ethylenediamine tetraacetic acid)
and Citric acid on the bioaccumulation efficiency of the plant
were investigated. Mustard plants were grown in soil around
steel industry. The results indicated significant reduction of
metals in the soil and increased accumulation in biomass. EDTA
proved better than citric acid in extraction of metals from the
soil. Order of percentage phytoextraction by plant was Fe+2 >Cd
>Al > Zn > Cr > Cu > Mn.
5. AREA AFFECTED WITH ARSENIC
Fifty districts of Bangladesh and 9 districts in West Bengal, India have
arsenic levels in groundwater above the maximum permissible limit of 50
μg/L . ( WHO )
7. CITITES AT RISK IN INDIA
LEAD CONTENT IN WATER
Alarming High Medium Low
Kolkata Delhi Chennai Bengaluru
Kochi Coimbatore Ludhiana Ahmedabad
Mumbai Madurai Surat Hyderabad
Pune Bhubaneswar Ghaziabad Indore
Nagpur Jamshedpur Bhopal
Nashik Chandigarh
Guwahati Lucknow
Mangalore
Mysore
8. Pb Hg Cd As
Ratlam
(M.P.)
Kodaikanal
(T.N.)
Kamrup,
Dhemaji
(Assam)
Tuticorin
(T.N.)
Bandalamottu Mines
(A.P.)
Ganjam
(orissa)
Pathanamthitta
(Kerala)
West Bengal
Vadodara
(Gujrat)
Singrauli
(M.P.)
Ballia
(U.P.)
Korba
(Chattisgarh)
HEAVY METALS CONTAMINATED AREA IN INDIA
Gautam SP, CPCB, New Delhi, Ram Murty, Indian Institute of Toxilogy Research
Buragohain et al.,2013, Shakhila et al.,2014
9. INTRODUCTION
All living creatures requires minerals
Naturally metals are distributed in environment during earth's
origin.
Rapid industrialization
Overgrowing urbanization
Environmental manipulation
(Jarup L, 2003; Waldron and Ediing,1997)
Threshold
level
Deficiency disease
Normal healthy life
Toxicity
10. Heavy Metals
Non essential
Ba, Li, Zr
Less toxic
Sn, Al
Highly toxic
Pb, Hg, Cd
Essential
Cu, Zn, Co,
Cr, Mn, Fe
On health effects basis
Metal having atomic weight greater than sodium (23) and specific
gravity (density) > 5gm/cm3 (Hollemen and Wiverd,1985)
(Mukesh K. Raikwar et al.,2008)
13. Secondary sources of heavy metals
Most of the animals affected
Industrial & Domestic wastage directly/indirectly release in water
(Pb, Hg, Cd, As) (Bell et al., 2001)
14. Contd...
Agriculture soil contaminated by Heavy metals through…
Long-term use of phosphatic fertilizers (Cd)
Sewage/sludge application (Hg, Cd)
Dust from smelters, industrial waste (Pb, Hg, Cd, As)
Bad watering practices in agricultural lands (Pb, Hg, Cd, As)
(Bell et al., 2001)
Plants are contaminated by Heavy metals through…
Excessive use of fertilizers/pesticides/insecticides
Plants growing in soil contaminated area
Irrigation of crop by contaminated water
18. HEAVY METALS IN AYURVEDIC MEDICINES
Karela tablets, produced by Shriji Herbal Products, India
Karela capsules, produced by Himalaya Drug Co, India
Karela capsules, produced by Charantia, UK (specifically batch #12011)
Maha Sudarshan Churna powder, produced by Zandu Pharmaceuticals,
Mumbai, India
Maha Sudarshan Churna powder, D & K Pharmacy, Bhavnagar, India
Maha Sudarshan Churna powder, produced by Chhatrisha, Lalpur, India
Maha Sudarshan Churna powder, produced by Dabur India Ltd, New
Delhi, India
SAFI liquid, produced by Hamdard-WAKF-Pakistan
SAFI liquid, produced by Hamdard-WAKF-India
Yograj Guggul tablets, produced by Zandu Pharmaceuticals, Mumbai,
India
Sudarshan tablets, produced by Zandu Pharmaceuticals, Mumbai, India
Shilajit capsules, produced by Dabur India Ltd, New Delhi, India
(WHO Drug Information Vol. 19, No. 3, 2005).
31. Mechanism underlying the development of
oxidative stress in a cell on lead exposure
(Gagan flora at al.,2012)
Under normal physiological conditions, there is a
balance between free radicals and antioxidants and
any deviation from it can cause oxidative stress
leading to cell death. (Gagan flora at
32. Cattle : show
head pressing
behaviour.
LEAD POISONING: CLINICAL
SIGNS
Cattle: advanced stages of lead poisoning,
become frenzied, bellow, stagger and crash
into obstacles
Gastrointestinal signs include colic, constipation for several days followed by
diarrhoea.
Abortion(mid or late gestation), opisthotonos, salivation, lacrimation and
paralysis may also be observed.
Death may occur within several hours or days. (O.M.Radostits et al. 10th
Ed.)
33. Mercury (Hg)
Minamata(1953--60) & Niigata(1964--65) disease in Japan
(Mottet et al, 1985)
Elemental-Hg – non toxic (orally), highly toxic(inhalation)
Inorganic-Hg – less toxic (insoluble < soluble)
Organic-Hg – more toxic (H.S.Sandhu,2nd ed,2012)
Liquid forms at room temp
Young ruminants more susceptible than Horse & Pig
(H.S.Sandhu,2nd ed,2012)
Se & Vitamin E protects against toxicity (Parizek et al., 1974)
Structural and functional disintegration of the enzymes
(–SH group) ( Roy Chowdhury A and Vachhzajani KD; 1987)
36. The intestinal uptake and subsequent distribution of organic mercurials, such as
methylmercury, throughout the body. a. Conjugation with glutathione (GSH), shown as
CH3—Hg—SG. b. Secretion of conjugate into bile. c. Reabsorption in gallbladder.
d. Remaining Hg enters intestinal tract.
37. The ability of organic mercurials to cross the blood–brain barrier and the placenta
contributes to their greater neurological and teratogenic effects when compared with
inorganic mercury salts. Note the structural similarity of the methylmercury complex to
methionine, CH3SCH2CH2—CH(NH3 +)COO–.
39. Mercurial
salts
stomatitis,pharyngitis,
vomiting,diarrhea
,dehydration, and shock.
Death may occur within
hours.
Oliguria and azotemia,
lasting for 1-2 days,
follow in animal animals
that survive acute
mercuric ion toxicosis
Alkyl mercurials(e.g.,methyl
mercury,ethyl mercury)
signs develop over a
period of 7-21 days.
Neurological signs
(depression,ataxia,Incoordination,
paresis, & blindness
Dermatologic
signs(e.g.,dermatitis,pustules, Ulcers)
Abnormal
postures,complete
blindness
Mercury Toxicity: Clinical
Signs
(O.M.Radostits et. al.,10th ed.
40. Regulatory limit in agricultural soil is 100 mg/kg soil.
(Salt et al., 1995)
Itai Itai disease
> 5ppm toxic effect
Most common in Ruminants (NRC,1980)
CADMIUM (Cd)
41.
42. Antagonistic activity against Cu, Zn, Se & Fe
(chemical similarities & competition for binding )
(Ammerman et al., 1973)
Oxidative stress
Destroy the SOD (Cd replaces Zn2+ )
(Zn maintain the str. of SOD that scavenges the FR)
(Darbre, 2006)
Inhibits the GSHB-Px
(catalyzed the destruction of H2O2 & LP & protects the lipids
membrane from peroxide damage)
Cd involved in Metal interaction
44. • Anemia
• Retarded growth
• Proteinuria
• Glycosuria,
• Hyperphosphatemia
• Testicular degeneration and necrosis
• Arthropathy and osteoporosis
• Vomition and diarrhoea in acute cases.
(O.M.Radostits et al. 10th Ed.)
Clinical Signs
45. More abundance in the Earth’s crust 1.5–3.0 mg/kg
(20th most abundant element)
(Mandal and Suzuki, 2002)
Used as first drug to cure syphilis by Paul Erlich
(Waxman and Anderson, 2001)
Most extensive exposure through drinking water
In Bangladesh 1980, arsenic-contaminated Artesian well water.
(Mandal and Suzuki, 2002)
Oxidative stress
Carcinogenicity
Arsenic
47. SUBACUTE:
Bloody diarrhoea & dehydration.
Weakness and hind limb
paralysis
Organic Arsenic: Blindness and incordination
mainly occur in overdosing of arsenilic acid.
In swine dog sitting posture
ARSENIC TOXICITY CLINICAL SIGNS
Chronic:
Low body weight &
sloughing of skin
(O.M.Radostits et al. 10th Ed.)
49. 1. Decontamination
• Removal of the patient from the source of exposure is critical to
limiting dose.
• Emetics, activated charcoal, gastric lavage employed if ingestion is
recent.
• Charcoal administered @1-4 mg/kg P/O.
2. Resuscitation
Good supportive care is critical.
3. Chelation
Basic principles of metal toxicity management :
(1) Prevention of further metal absorption into the system
(2) Elimination of metal from the circulation
(3) Inactivation of metal bioavailable in the system
50. • Chelation has its origin in the Greek word chele that means claw of a
lobster, thus depicting the concept of clinging or holding with a strong
grip.
• The term chelate was first applied by Sir Gilbert T. Morgan and H. D. K.
Drew in 1920.
They suggested the term for the caliper-like groups which function as two
associating units and fasten to a central atom so as to produce heterocyclic
rings
(T. Morgan et. al.,1920)
CHELATION
53. Edetate Calcium Disodium
Treatment of poisoning by metals that have
higher affinity for the chelating agent than does
ca2+.
EDTA is charged at physiological pH, it does not
significantly penetrate cells; its volume of
distribution approximates extracellular fluid space.
Lead Poisoning.
• Bone provides the primary source of lead that is chelated by CaNa2EDTA
• After such chelation, lead is redistributed from soft tissues to the skeleton
• Calcium versenate (Ca Na2 EDTA, Ca EDTA) @ 110-220 mg/kg BW IV infusion, 2
times a day (as 1-2% solution in 5% dextrose) for 4-5 days ( Large animals)
(CALCIUM DISODIUM VERSENATE)
• Mercury is unavailable to the chelate perhaps because it is too tightly bound
by sulfhydryl groups or sequestered in body compartments that are not
penetrated by CaNa2EDTA.
Toxicity: hypocalcemic tetany, hydropic vacuolization of the proximal tubule, loss of the brush
border, and eventually, degeneration of proximal tubular cells (Catsch and Harmuth- Hoene,
54. • Like EDTA, is a polycarboxylic acid chelator, but it has somewhat greater
affinity for most heavy metals.
Pentetic Acid (DTPA/ Di ethylene tri amine penta acetic acid)
• Limited access to intracellular sites of metal storage
• Because DTPA rapidly binds ca2+, CaNa3DTPA is employed
Heavy-metal poisoning that do not respond to EDTA, particularly poisoning by
radioactive metals like Uranium and Plutonium (N.L.Spoor, 1977)
Disadvantage of depleting zn from the system that may be overcome by supplementation
or using the zinc salt of the drug.
Teratogenic like CaNa2EDTA due to its Zn and Mn depletion effect
55. Developed during world war II as an antidote to lewisite, a
vesicant arsenical war gas, hence its alternative name,
british antilewisite (BAL).
Dimercaprol(2,3dimercaptopropanol)
Its instability in aqueous solutions, peanut oil is the solvent employed in
pharmaceutical preparations.
Arsenicals would form a very stable and relatively nontoxic
chelate ring with the dimercaprol
MOA: Formation of chelation complexes Between its sulfhydryl groups and metals
• Antagonizes the biological actions of metals that form mercaptides with essential
cellular sulfhydryl groups, principally arsenic, gold, and mercury.
The sulfur–metal bond may be labile in the acidic tubular urine, which may increase
Delivery of metal to renal tissue and increase toxicity.
Maintain a concentration of dimercaprol in plasma adequate to favor the continuous
formation of the more stable 2:1 (BAL–metal) complex and its rapid excretion
56. More effective in preventing inhibition of sulfhydryl enzymes than in reactivating them.
Used in combination with CaNa2EDTA to treat lead poisoning, especially when
evidence of lead encephalopathy exists.
Dimercaprol cannot be administered orally; it is given by deep intramuscular injection
as a 100 mg/ml solution in peanut oil,
Toxicity: rise in systolic and diastolic arterial pressures, accompanied by tachycardia
Arsenic toxicity: BAL(British Anti-lewisite)/Dimecaprol:@4-7mg/kg I/M
t.i.d×3days.
57. An orally effective chelator that is chemically similar to dimercaprol but contains two
carboxylic acids that modify both the distribution and chelating spectrum of the drug.
Succimer (2,3-dimercaptosuccinic acid, CHEMET)
After Absorption
Effective as a chelator of arsenic, cadmium, mercury, and other metals
(Aposhian and Aposhian, 1990)
Toxicity : less than that with dimercaprol perhaps because its relatively lower lipid
solubility minimizes its uptake into cells
A desirable feature : it does not significantly mobilize essential metals such as zinc,
copper, or iron.
58. First isolated in 1953 from the urine of patients with liver
disease who were receiving penicillin
Penicillamine (D-β,β-dimethylcysteine)
Effective chelator of copper, mercury, zinc, and lead and
promotes the excretion of these metals in the urine.
Absorbed (40% to 70%) from the GI tract
N-Acetylpenicillamine is more effective than penicillamine in protecting against the
toxic effects of mercury presumably because it is even more resistant to metabolism.
Toxicity. With long-term use, induces several cutaneous lesions, including urticaria,
macular or papular reactions, pemphigoid lesions, lupus erythematosus,
dermatomyositis, adverse effects on collagen,
59. Chelation
Monotherapy
Combination
Therapy
Antioxidents
Micronutrients
Phytochemicals
Acute Metal Exposure
Soft Tissues
Cellular Manifestation
Pro – vs antioxident imbalance
Metabolic pathway interfered
(haem synthetic pathway)
Tissue Damage & Organ
dysfunction
Cellular Manifestation
Oxidative Stress, Pro- or Anti-
apoptotic manifestations
(Mitochondrial dysfunction, DNA
damage, etc)
Systemic Manifestations
Disease induction or promotions
(Diabetes,Cancer, DVD, etc)
Soft & Hard Tissues
Chronic Metal Exposure
Excretion (Urinary / Biliary)
61. • Greater Affinity, Low Toxicity
•Ability to compete with natural chelators
•Ability to penetrate cell membranes
•Rapid elimination of the toxic metal
•High water solubility
•Capacity to form non-toxic complexes
•Same distribution as the metal
(Swaran J.S. Flora and Vidhu Pachauri ,
IDEAL CHELATER
62. Benefits
• Effective against
acute poisoning
• Form non-toxic
complexes
• Remove metal from
soft tissues
• Oral therapy is
available
Drawbacks
• Redistribution of toxic metal
• Essential metal loss
• No removal of metal from
intracellular sites
• Hepatotoxicity and
nephrotoxicity
• Poor clinical recovery
• Pro-oxidant effects (DTPA)
• Headache, nausea, increased
blood pressure
CHELATION
64. BIOREMEDIATION
Use of different biological systems to destroy or reduce concentrations of
contaminants from polluted sites.
Microbes and plants have a natural capability to attenuate or reduce: Mass,Toxicity,
Volume, Concentration of pollutants
Aerobic bacteria:
Examples include: Pseudomonas, Alcaligenes, Sphingomonas, Rhodococcus, and
Mycobacterium.
Fungi:
Able to degrade a diverse range of persistent or toxic environmental pollutants
(Bodishbaugh, D.F., 2006)
Phytoremediation is the use of living green plants for in situ risk reduction and/or
removal of contaminants from contaminated soil, water, sediments, and air
Hyper accumulator plant species are used on many sites due to their tolerance of
relatively extreme levels of pollution.
Avena sp. , Brassica sp.
65.
66.
67. BIOREMEDIATION
Use of different biological systems to destroy or reduce concentrations of
contaminants from polluted sites.
Microbes and plants have a natural capability to attenuate or reduce: Mass,Toxicity,
Volume, Concentration of pollutants
Aerobic bacteria:
Examples include: Pseudomonas, Alcaligenes, Sphingomonas, Rhodococcus, and
Mycobacterium.
Fungi:
Able to degrade a diverse range of persistent or toxic environmental pollutants
(Bodishbaugh, D.F., 2006)
Phytoremediation is the use of living green plants for in situ risk reduction and/or
removal of contaminants from contaminated soil, water, sediments, and air
Hyper accumulator plant species are used on many sites due to their tolerance of
relatively extreme levels of pollution.
Avena sp. , Brassica sp.