The document outlines a laboratory curriculum focused on hamster genetics and biotechnology, where students will apply the scientific method to study inheritance patterns using Syrian hamsters. Key objectives include determining phenotypes and genotypes through breeding, while adhering to ethical treatment of lab animals. It also provides detailed genetic information about hamsters, including coat colors, types, and breeding techniques, emphasizing careful selection for superior breeding outcomes.

1. Hamster Genetics Biotechnology – Inheritance and Genetics Applications

2. Standards Addressed: 15.0 Animal Growth and Production 15.3 Reproduction 15.4 Biotechnology – 16.0 Using Scientific Processes to Analyze Data 16.1 Scientific Method 16.2, 16.3, 16.4 Scientific Research 16.6, 16.8 Communicating Results 17.0 Relationships Within Living Systems 17.2 Heredity 18.0 Bioethical Issues 18.3 Animal production

3. Objectives: In a Laboratory Environment Students will be able to: 1- Use the scientific method to hypothesize, predict implement and report conclusions 2 – Illustrate patterns of inheritance using the principles of Mendel’s genetic theory 3 – Demonstrate respectful, and appropriate treatment of lab animals

4. Process: Using Syrian Hamsters students will identify phenotype of animal. Through multi generation breeding the genotype will be determined As genotype becomes established, predictions regarding future generations are made, tested, and results reported

5. Why Use Syrian Hamsters? 1 – A friendly, affordable, easily available animal 2 – Rapid maturation of pups 3 – Easily bred – short estrous cycle – 4 days 4 – Gestation period 16 days 5 – Established coat color, coat type, and coat markings 4 – Monohybrid and dihybrid crosses visible

6. Careful, monitored breeding will result in pedigree charts

7. Genetic Predicting Helps Determine Future Offspring

8. Safe, humane lab animal care is basic

9. Basic Genetic Information: Hamsters have 44 chromosomes Ovaries carry 21 autosomes and an X gamete Sperm carry 21 autosomes and an X or Y gamete Syrian hamster Karyotype courtesy of Knud Christensen, Ph.D., The Royal Veterinary Agricultural University, Denmark

10. Step 1: figure out the hamster's phenotype, and write a preliminary genotype. A few quick examples. ("_" stands for "unknown.") Banded Longhair Red Eyed Cream Ba_eellpp Satin Cinnamon Tortoiseshell ppSasaToto Dominant Spot Yellow Black male aaDsdsToY Rex Smoke Pearl female dgdgrxrxToTo Chocolate (Sable) Roan bbeeU_ Whwh Recessive Dappled Umbrous Golden rdrdU_

11. Expanding the Genotype More information about the hamster's genotype can be gathered by looking at its parents, siblings and offspring

12. Breeding an animal can provide more data about its genetic make-up. It is especially useful as a tool to confirm suspicions of heterozygosity. For any trait that may be heterozygous, breed to an animal that is homozygous recessive.

13. Appropriate breeding can help sort out confusing colors. For example this helps with the Greys, especially if the Grey is in combination (for instance, in the Grey Sables or Black Eyed Ivories). Breeding to any hamster without grey in its pedigree will give a good indication of the genotype.

14. There are eleven basic colors in Syrians. Ten (excluding Golden, which is the “wild type”) are determined by a single gene pair .

15. It can be hard to determine color because coat types and coat patterns also influence appearance.

16. Golden – wild color combination – where it all started “ Ticking” of coat And, the fur is often different close to the skin, from what is seen on top -

17. Determining the Coat Color Phenotype

18. Color Genotype Description Golden ++ Rich dark mahogany heavy black ticking OR Light fawny gold Golden is the agouti, or wild code. It must carry all color genes possible, so would look like this: AA BB baba CC DgDg dsds EE FdFd LL lglg PP RdRd RuRu RxRx SS sasa sgsg toto uu whwh

19. Color Genotype Description Black aa Jet black to the roots

20. Black Eye ee Deep sandy cream Cream to the roots Color Genotype Description

21. Color Genotype Description Cinnamon pp Rich russet orange

22. Color Genotype Description Dark Ear cdcd White to the roots White

23. Color Genotype Description Extreme cece Creamy grey with Dilute black ticking

24. Color Genotype Description Dark Grey dgdg Pearly grey, black ticking, black eye rings

25. Color Genotype Description Light Grey Lglg Buttermilk grey dark ticking

26. Color Genotype Description Rust bb orange brown ticked with brown

27. Color Genotype Description Silver Grey SgSg Soft pastel grey ticked brownish black

28. Color Genotype Description Yellow ToTo or ToY Dusky yellow with heavy black ticking

29. Color Tortoiseshell (females only) TotoBaba, TotoBaBa, or TotoDsds sex-linked bi-colored or tricolor animal - a balanced pattern of colored, yellow and white patches (sometimes black) Genotype Description

30. Coat Type Another gene we will select for - 5 Coat types exist -

31. Shorthair ++ Wild type coat – short, even dense

32. Longhair ll Longer hair over top, excluding face, much greater in males, not as much ticking

33. Rex rxrx Wavy, frizzy, diluted color, curly whiskers

34. Satin Sasa Glossy sheen, shiny

35. Hairless hrhr No fur, may have curly whiskers

36. Fur Patterns 8 Specific fur pattern types exist

37. Banded BaBa Centrally placed band of white (1/3 of body) white belly

38. Dominant Spot Dsds White with colored spots, white face blaze, white belly

39. Recessive Dappled rdrd Description: White with colored face, rear brindled splotches, shoulders white, white face blaze, white belly

40. Roan Whwh White with colored hairs ticked through coat, more color on head, white belly

41. White Bellied WhWh white ticking, especially on face

42. Piebald ss Similar to dominant spot, but has colored patches also on belly

43. Color Tortoiseshell (females only) TotoBaba, TotoBaBa, or TotoDsds sex-linked bi-colored or tricolor animal - a balanced pattern of colored, yellow and white patches (sometimes black) Genotype Description

44. The yellow patches in Tortoiseshell are modified according to base color – Cinnamon becomes honey – Dark Grey becomes pearl – Lilac becomes lilac pearl Remember – this is a sex linked trait – only in females!

45. There are two main breeding techniques - selection and crossing. Selection is the careful choosing of individuals for mating, allowing only the best to become parents of the next generation.

46. Crossing , is the pairing of the selected individuals so that the best offspring possible can be produced.

47. Inbreeding involves crosses of first degree relatives. This is done to concentrate desirable genes. Unfortunately, it will also concentrate undesirable genes so it must be accompanied by rigorous selection and care.

48. Backcross (offspring bred to one of its parents) Do with care and only 1 generation Sibling cross (brother bred to sister – the most unsafe – only unusual circumstances (verify genetics_

49. Linebreeding is the pairing of more distantly related animals. Cousin to cousin – Aunt to nephew, uncle to niece -considered fairly safe

50. Outcrossing is the breeding of totally unrelated animals. Done when 2 exceptional, unrelated animals exist. Very safe.

51. Lethality Lethal genes result in death in utero or Shortly after birth. Lg (Light, or Lethal, Grey) and Ds (Dominant Spot) are lethal in homozygotes. In a monohybrid cross (e.g., Lglg x Lglg) 1/4 of the embryos are likely to be homozygous dominant and will not survive. The litter is then three fourths as large as it would have been and has the remaining genotype (and phenotype) ratio of 2:1. Since the surviving hamsters are healthy, the only disadvantage of these matings is smaller litters.

52. Mating two hamsters that are Roan or White Bellied (both Whwh) gives litters in which 1/4 of the babies can be expected to be Eyeless Whites (WhWh). Eyeless Whites typically have a shortened lifespan of six to twelve months.

53. Principles: Careful Breeding Results In Superior Animals! Breed for Fun, Knowledge, and Improved Species.

54. Produced for: Santa Rita High School Agriscience Program By: Catherine Patterson, Instructor Special Thanks To: River Road Hamstry and the many owners and breeders of Hamsters who placed information on the web.