This study investigated whether supplementing glutathione (GSH) could attenuate lipopolysaccharide (LPS)-induced mitochondrial dysfunction and apoptosis in a mouse model of acute lung injury (ALI). The researchers found that LPS increased oxidative stress, mitochondrial dysfunction, and apoptosis in mouse lung tissue. However, pre-treating the mice with GSH-ethyl ester prevented the LPS-induced increases in oxidative stress, preserved mitochondrial function, and reduced apoptosis. Thus, GSH supplementation may protect against LPS-induced mitochondrial damage and cellular apoptosis in ALI.
"Mechanisms of nitric oxide synthase uncoupling in endotoxin-induced acute lung injury: role of asymmetric dimethylarginine" appeared in a 2010 issue of Vascular Pharmacology and summarized Stephen M. Black's research into acute lung injury/sepsis.
This document reviews the role of reactive oxygen species (ROS) in muscle function and how they may contribute to age-related sarcopenia. ROS are produced in muscles through various pathways including the mitochondrial electron transport chain and NADPH oxidase enzymes. At low levels, ROS play a role in muscle force production and exercise adaptation, but high levels can damage muscles. In aging muscles, ROS levels increase due to reduced antioxidant defenses and may cause cumulative oxidative damage, contributing to sarcopenia through impaired muscle mass, strength, and function.
1) Rates of ubiquitin conjugation (attachment of ubiquitin molecules) to proteins increase in muscles undergoing atrophy (wasting away), largely due to activation of the N-end rule pathway.
2) The N-end rule pathway is responsible for most changes in ubiquitin conjugation rates in atrophying muscles from tumor-bearing or septic rats. Inhibitors of this pathway suppressed the increased ubiquitin conjugation seen in these conditions.
3) Ubiquitin conjugation rates increase in atrophying conditions like cancer cachexia and sepsis but decrease in hypothyroid muscles, and this response is mediated by the N-end rule pathway.
1) ADMA levels are significantly elevated in patients with severe sepsis compared to healthy controls and correlate with severity of organ failure and mortality.
2) Higher ADMA levels on day 1 are associated with need for inotropic support and higher levels on day 7 correlate with increased mortality.
3) While CRP and ADMA both increase in sepsis, only ADMA levels correlate with severity of organ dysfunction.
Presentation format4 posttranslational modification in cell adhesion and migr...Birgit Kastberger
Posttranslational modifications (PTMs), such as phosphorylation, acetylation, and methylation regulate cell adhesion and migration. PTMs modify integrin function and the activity of proteins involved in cytoskeletal dynamics like cofilin, alpha-tubulin, cortactin, and EF1a1. Acetylation of cortactin and alpha-tubulin reduces cell migration by decreasing their association with actin and microtubule stability, respectively. Phosphorylation of cofilin regulates its actin binding activity and ability to sever actin filaments. Methylation of EF1a1 is required for neural crest cell migration. Drugs that alter the cell's acetylation state through inhibition of HDACs or modulation of acetyl-
The document summarizes research investigating the effects of (-)-epicatechin, a flavanol antioxidant found in cocoa, on gene expression in the yeast Saccharomyces cerevisiae. Yeast were exposed to increasing concentrations of (-)-epicatechin and changes in expression of genes related to longevity and oxidative stress were analyzed. Preliminary results found that a longevity gene (HST2) was upregulated at higher concentrations, while an oxidative stress gene (GSH1) was downregulated, supporting the hypotheses. Future research is proposed to test additional genes and a higher concentration range to better understand how (-)-epicatechin impacts longevity and oxidative stress.
Acute L-Arginine supp has no effect on CV or thermoreg resp to rest, ex and r...Thomas Coffey
This study investigated the effects of acute l-arginine (L-ARG) supplementation on cardiovascular and thermoregulatory responses to rest, exercise, and recovery in the heat. Eight healthy males completed two trials where they consumed either 10g of L-ARG or a placebo beverage before a 90 minute experiment in 35°C heat. L-ARG supplementation increased plasma L-ARG concentrations but had no effect on rectal temperature, skin temperature, heart rate, blood pressure, skin blood flow, oxygen consumption, or sweat loss at rest, during exercise, or recovery compared to placebo. The study found that acute L-ARG supplementation provided no additional thermoregulatory or cardiovascular benefits during passive or active heat exposure in healthy individuals
Chemical-quantum determination of the interaction type of leflunomide and col...IJAEMSJORNAL
In recent years, researchers have evaluated the clinical efficacy and safety of Leflunomide (LEF) and other medications for the treatment of rheumatoid arthritis (RA). The objective of this work was chemical-quantum calculus and determination of the interaction type of LEF and collagen and its influence on arthritis. We obtained the information about the collagen of The National Center for Biotechnology Information (NCBI). It was obtained the formula of the LEF on the page of PubChem. The quantum calculations we carry out with the hyperchem software. These calculations were made in other articles already published by us. We concluded that 13 interactions of 40 calculated are significant. These 13 interactions show that only two of them are oxidant and the other 11 are reductive.
"Mechanisms of nitric oxide synthase uncoupling in endotoxin-induced acute lung injury: role of asymmetric dimethylarginine" appeared in a 2010 issue of Vascular Pharmacology and summarized Stephen M. Black's research into acute lung injury/sepsis.
This document reviews the role of reactive oxygen species (ROS) in muscle function and how they may contribute to age-related sarcopenia. ROS are produced in muscles through various pathways including the mitochondrial electron transport chain and NADPH oxidase enzymes. At low levels, ROS play a role in muscle force production and exercise adaptation, but high levels can damage muscles. In aging muscles, ROS levels increase due to reduced antioxidant defenses and may cause cumulative oxidative damage, contributing to sarcopenia through impaired muscle mass, strength, and function.
1) Rates of ubiquitin conjugation (attachment of ubiquitin molecules) to proteins increase in muscles undergoing atrophy (wasting away), largely due to activation of the N-end rule pathway.
2) The N-end rule pathway is responsible for most changes in ubiquitin conjugation rates in atrophying muscles from tumor-bearing or septic rats. Inhibitors of this pathway suppressed the increased ubiquitin conjugation seen in these conditions.
3) Ubiquitin conjugation rates increase in atrophying conditions like cancer cachexia and sepsis but decrease in hypothyroid muscles, and this response is mediated by the N-end rule pathway.
1) ADMA levels are significantly elevated in patients with severe sepsis compared to healthy controls and correlate with severity of organ failure and mortality.
2) Higher ADMA levels on day 1 are associated with need for inotropic support and higher levels on day 7 correlate with increased mortality.
3) While CRP and ADMA both increase in sepsis, only ADMA levels correlate with severity of organ dysfunction.
Presentation format4 posttranslational modification in cell adhesion and migr...Birgit Kastberger
Posttranslational modifications (PTMs), such as phosphorylation, acetylation, and methylation regulate cell adhesion and migration. PTMs modify integrin function and the activity of proteins involved in cytoskeletal dynamics like cofilin, alpha-tubulin, cortactin, and EF1a1. Acetylation of cortactin and alpha-tubulin reduces cell migration by decreasing their association with actin and microtubule stability, respectively. Phosphorylation of cofilin regulates its actin binding activity and ability to sever actin filaments. Methylation of EF1a1 is required for neural crest cell migration. Drugs that alter the cell's acetylation state through inhibition of HDACs or modulation of acetyl-
The document summarizes research investigating the effects of (-)-epicatechin, a flavanol antioxidant found in cocoa, on gene expression in the yeast Saccharomyces cerevisiae. Yeast were exposed to increasing concentrations of (-)-epicatechin and changes in expression of genes related to longevity and oxidative stress were analyzed. Preliminary results found that a longevity gene (HST2) was upregulated at higher concentrations, while an oxidative stress gene (GSH1) was downregulated, supporting the hypotheses. Future research is proposed to test additional genes and a higher concentration range to better understand how (-)-epicatechin impacts longevity and oxidative stress.
Acute L-Arginine supp has no effect on CV or thermoreg resp to rest, ex and r...Thomas Coffey
This study investigated the effects of acute l-arginine (L-ARG) supplementation on cardiovascular and thermoregulatory responses to rest, exercise, and recovery in the heat. Eight healthy males completed two trials where they consumed either 10g of L-ARG or a placebo beverage before a 90 minute experiment in 35°C heat. L-ARG supplementation increased plasma L-ARG concentrations but had no effect on rectal temperature, skin temperature, heart rate, blood pressure, skin blood flow, oxygen consumption, or sweat loss at rest, during exercise, or recovery compared to placebo. The study found that acute L-ARG supplementation provided no additional thermoregulatory or cardiovascular benefits during passive or active heat exposure in healthy individuals
Chemical-quantum determination of the interaction type of leflunomide and col...IJAEMSJORNAL
In recent years, researchers have evaluated the clinical efficacy and safety of Leflunomide (LEF) and other medications for the treatment of rheumatoid arthritis (RA). The objective of this work was chemical-quantum calculus and determination of the interaction type of LEF and collagen and its influence on arthritis. We obtained the information about the collagen of The National Center for Biotechnology Information (NCBI). It was obtained the formula of the LEF on the page of PubChem. The quantum calculations we carry out with the hyperchem software. These calculations were made in other articles already published by us. We concluded that 13 interactions of 40 calculated are significant. These 13 interactions show that only two of them are oxidant and the other 11 are reductive.
This document contains abstracts from the 2005 SWACSM Annual Meeting. The summaries are:
1) A study found that inhibiting ERK1/2 prevented the contraction-induced increase in muscle fatty acid metabolism and CD36 content in a dose-dependent manner, without affecting AMPK activity. ERK1/2 phosphorylation was positively correlated with fatty acid uptake and oxidation. This suggests ERK1/2 is required for contraction-induced increases in fatty acid metabolism.
2) A study found that inhibiting various potassium channels individually did not alter vascular tone in soleus feed arteries, but inhibiting inward rectifier potassium channels and the sodium-potassium pump together significantly increased vascular tone. This suggests these channels contribute to
L'idrossitirosolo è in grado di proteggere e aumentare l'attività dei mitocon...CreAgri Europe
L'idrossitirosolo, un polifenolo estratto dalle olive, attraverso una protezione dei mitocondri, le centrali energetiche della cellula, è in grado di aumentare la resistenza muscolare negli animali da esperimento
Dietary Supplementation with Calcium in Healthy Rats Administered with Artemi...IOSR Journals
Reports on the role of calcium on predisposition to cardiovascular disease have been rather inconsistent while studies on its interaction with other medications are ongoing. We therefore investigated the effect of separate and combine administration of calcium supplement with artemisinin-based combination drug on hepatic and serum lipid profile. Thirty two male wistar rats were randomly assigned into four groups of eight rats each. The control (group A) received normal saline. Group B and D were placed on 10mg/Kg calcium twice daily for four weeks. On the thirtieth day, therapeutic dose of artemisinin-based combination was simultaneously administered to group C and group D twice daily for three days. All the rats were then sacrificed after 12 hours fasting, blood was withdrawn and the liver removed and homogenized in an appropriate buffer. Biochemical analysis showed no significant (p>0.05) variation in hepatic triaacylglycerol in all the treated groups whereas calcium supplementation was observed to induce a significant (p<0.05) reduction in hepatic cholesterol. Significant elevations due to calcium supplementation were also observed in serum total cholesterol, LDL cholesterol level and atherogenic risk index with a concomitant reduction in serum HDL cholesterol. No significant change was observed in serum total cholesterol, triacylglycerol and serum lipoproteins in all other treatment groups. Our study suggests that calcium supplementation may predispose to cardiovascular disease and that its co administration with ACT may not aggravate nor reduced the predisposition risk.
The document discusses DNA repair mechanisms in human lymphocytes. It describes how oxidized purines in DNA are repaired through the base excision repair pathway involving the OGG1 glycosylase. This repair requires activation by the transcription factor NF-YA, which upregulates OGG1 expression when DNA is at risk of oxidation. The repair of oxidized bases by OGG1 in human lymphocytes is slow but can be activated by phytohemagglutinin. Disruption of this global repair of oxidized purines may be due to downregulation of OGG1. A second document discusses how green tea acts as an antioxidant to protect against oxidative stress and influence DNA damage and repair through activation of the Nrf2 transcription factor and upreg
1) The researchers generated mutations of the I-F11 nerve agent bioscavenger enzyme that eliminated glycosylation by mutating asparagine residues 253 and 324.
2) Testing showed the double mutant had increased thermal stability in vitro but no change in circulatory half-life in vivo compared to the glycosylated wild type.
3) Mutation of either residue 253 or 324 alone resulted in partial deglycosylation, while mutation of both residues eliminated glycosylation, indicating these are the sites of glycosylation in the enzyme.
Acute and chronic hyperammonemia modulate antioxidantrkkoiri
1) The study examines the effects of acute and chronic hyperammonemia on antioxidant enzyme levels and oxidative damage in the cerebral cortex and cerebellum of rats.
2) Acute hyperammonemia decreased antioxidant enzyme levels in both brain regions but increased lipid peroxidation only in the cerebellum.
3) Chronic hyperammonemia increased antioxidant enzyme levels in the cerebral cortex but decreased levels in the cerebellum, suggesting the cerebellum is more susceptible to oxidative stress during chronic hyperammonemia.
This document describes a project to develop an in vitro lipoylation reaction to screen chemicals hypothesized to trigger primary biliary cirrhosis (PBC) through molecular mimicry. The student conducted a series of optimization reactions altering amounts of lipoic acid, GTP/ATP, or lipoate activating enzyme and lipoyltransferase. Little success was achieved and time did not allow screening of chemicals, requiring further work. In conclusion, both enzymes are required for lipoylation but varying amounts did not greatly affect the reaction, prompting questions about enzyme purification for future studies.
This document summarizes a study examining the role of platelet-derived growth factor (PDGF)-evoked calcium signaling in human pulmonary fibroblasts. The study found that both acute and overnight PDGF treatment evoked calcium waves in these cells. Blocking external calcium, internal calcium stores, or phospholipase C inhibited the PDGF-evoked calcium waves and PDGF-induced expression of fibronectin and collagen genes. The results indicate that in human pulmonary fibroblasts, PDGF acts through IP3-induced calcium release from internal stores to trigger calcium waves, which then modulate expression of extracellular matrix genes.
Vitamin D plays an important role in regulating calcium and phosphorus balance and promoting bone growth. It also has immunomodulating, anticancer, and neuromodulating effects. The document reviews vitamin D's structure, metabolism, mechanisms of action, and functions in the body. It then discusses recent research on vitamin D's impact on oral cavity diseases, focusing on its potential role in autoimmune conditions of the oral mucosa like recurrent aphthous stomatitis.
This study investigated the effects of inhaling residual oil fly ash (ROFA) particles on oxidative stress and hemodynamics in exercising rats. The key findings were:
1. Rats that inhaled ROFA before exercise showed increased lipid peroxidation (a marker of oxidative stress) in the lungs and heart compared to those that inhaled saline.
2. However, ROFA inhalation did not significantly alter heart rate, blood pressure, or other hemodynamic responses during exercise compared to saline.
3. While ROFA promoted oxidative stress in the lungs and heart, it did not produce significant changes in cardiovascular function during swimming exercise in rats.
It is well established that muscle contraction during exercise lead to elevated levels of reactive oxygen species in skeletal muscle ,hence the growing interest of oxidative stress in exercise
The Role of IL-17, Metaphase Reactants on Patients with Early Rheumatoid Arth...IOSR Journals
Rheumatoid arthritis (RA) represents the most common form of chronic inflammatory joint disease leading to cartilage and bone destruction, It affects approximately 1-2 % of world’s population. The inflammatory process causes diffuse thickening and hyperplasia of the joint Interleukin (IL)-17 is a pleiotropic pro-inflammatory cytokine produced from Th17 cells. IL-17A is the prototypic member belonging to a family of 6 ranging from IL-17A to IL-17F. IL-17A mediates its biological effects through binding to a receptor complex consisting of IL-17RCA and IL-17RCC subunits.
Objective. To assess the role of IL-17 on the disease process of Rheumatoid arthritis and the effects on the trace elements such as Zinc, copper , Magnesium.
Subjects and method: A total of 60 patients with early Rheumatoid Arthritis were studied. Their ages ranged from 20-52 years with a mean age of ( 39.3 ± 9.01)years. , while the range was between (20-51) years and the mean was (37.5 ± 8.6) years for healthy control non significant difference P > 0.4 with no complaint of other chronic or systemic diseases were considered as control. the samples were collected during period from ( December 2012 – july 2013 ). the mean disease duration of RA was (3.27±1.2) month. Blood samples were collected from patients and controls to assess Erythrocyte Sedimentation Rate (ESR) and serum levels of White blood cells ( WBC), Hemoglobin (Hb), Interleukin-17A and were estimated by agglutination test, and IL-17A was estimated by (ELIZA).
Corticosteroid induced disorders – an overviewpharmaindexing
This document provides an overview of corticosteroid-induced disorders and adverse effects. It discusses the mechanism of action of corticosteroids and their effects on muscle, including causing muscle atrophy primarily through increased protein breakdown and inhibition of protein synthesis. It also mentions other corticosteroid-induced disorders like osteoporosis, glaucoma, diabetes, psychosis, and Cushing's syndrome. Prevention of corticosteroid-induced muscle atrophy through growth factors, amino acids, glutamine, taurine, and creatine is also summarized.
The document discusses the immune system and inflammation. It begins with an overview of the cellular components of the immune system, including macrophages, monocytes, basophils, eosinophils, neutrophils, and lymphocytes. It then discusses the process of exercise-induced muscle damage and the subsequent muscle inflammation and regeneration process. This involves leukocyte infiltration, cytokine production, phagocytosis to remove damaged muscle fibers, and satellite cell proliferation to aid regeneration over 1-2 weeks after exercise. NSAIDs are shown to inhibit this inflammatory process and blunt the activity of satellite cells that aid muscle repair.
This document describes a study probing the catalytic mechanism of the insulin receptor kinase (IRK) using a tetrafluorotyrosine-containing peptide substrate. Key findings include:
1) pH-rate profiles indicate the neutral phenol, not the phenoxide ion, is required for phosphorylation, supporting a dissociative transition state.
2) The pKa of the tetrafluorotyrosyl hydroxyl is elevated on the enzyme, and the phenoxide anion behaves as a weak competitive inhibitor.
3) Crystal structures show hydrogen bonding of the tetrafluorotyrosyl group to enzyme residues, consistent with its elevated pKa.
The imbalance between free radical production and endogenous antioxidant defence may result in cellular oxidative stress, causing oxidative damage to various cellular components, such as DNA, proteins and membrane lipids. The human system employs the use of endogenous enzymatic and non-enzymatic antioxidant defence systems against the onslaught of free radicals and oxidative stress.
Unsurprisingly, oxidative damage has been implicated in and is believed to be a key factor causing various pathological conditions, such as cardiovascular disease, neurodegenerative disease, diabetes and cancer. Free radicals can be quenched through a number of mechanisms. Antioxidants directly scavenge free radicals (e.g., via hydrogen atom transfer or electron transfer), prevent free radical formation by chelating metal ions and by interrupting the radical chain reactions of lipid peroxidation, thus retarding its progression. Enzymatic antioxidants include superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase. Non-enzymatic antioxidants include vitamins A, C, and E, glutathione, alpha-lipoic acid, carotenoids, and coenzyme Q10. Other antioxidants include polyphenols, minerals (copper, zinc, manganese, and selenium), and cofactors (B-vitamins). Together, antioxidants work synergistically with each other using different mechanisms against different free radicals and stages of oxidative stress.
The benefits associated with antioxidants are numerous and diverse but it can be a minefield when choosing the appropriate antioxidant support for clients. In this hour-long webinar, Dr Nina Bailey discusses the direct and indirect benefits and actions of key antioxidants including (but not limited to) astaxanthin, alpha lipoic acid, polyphenols and co-enzyme Q10, with a focus on:
-Antioxidant sources and benefits
-Mechanisms and actions
-When to combine antioxidants for synergistic effects
-Overcoming bioavailability issues
-Targeted intervention, which antioxidant(s) and why
The document summarizes the work of the Linus Pauling Institute at Oregon State University over the past 13 years. It discusses the Institute's focus on determining the roles of micronutrients, antioxidants, and other dietary factors in promoting optimal health and preventing disease. Key areas of research include cancer prevention, cardiovascular health, aging, and the protective effects of certain foods/compounds against oxidative stress and inflammation. The Institute has grown from half a researcher in 1996 to 12 full researchers and 4 research assistants currently. The work described emphasizes the important influence of diet and lifestyle on gene expression and chronic disease risk, beyond just genetic factors.
This document summarizes the current state of antifungal drug development and resistance mechanisms. It discusses the classes of antifungal drugs including azoles, polyenes, echinocandins, which act by inhibiting ergosterol biosynthesis or glucan synthesis. Resistance occurs via overexpression of drug targets, efflux pumps, or biofilm formation. Improving drug formulations, combinations, and developing new antifungals are important to address the rise in antifungal infections and resistance.
1) The study examined the effects of diethyl hexyl phthalate (DEHP) exposure on insulin signaling molecules and glucose metabolism in the triceps muscle of rats.
2) Rats treated with DEHP showed increased oxidative stress, reduced insulin receptor and glucose transporter 4 (GLUT4) levels, and decreased glucose uptake and oxidation in the triceps muscle.
3) Co-treatment with antioxidants vitamins C and E prevented the adverse effects of DEHP by reducing oxidative stress, and protecting insulin signaling molecule levels and glucose metabolism in the triceps muscle.
This study investigated the protective and curative effects of glycyrrhizin (GL) in a lipopolysaccharide (LPS) and D-galactosamine (D-GaIN)-induced acute hepatitis rat model. Rats were divided into groups: control, hepatitis model, GL pretreatment, and GL posttreatment at 1/2 and 4 hours. GL pretreatment significantly improved liver histology and reduced serum ALT and AST levels compared to the hepatitis model. GL posttreatment also showed reductions, with greater effects at 1/2 hour versus 4 hours. Immunohistochemistry revealed GL decreased caspase-3 and TNF-α expression, indicating anti-apoptotic and anti-inflammatory effects. The study
1) The study investigated the redox status of patients with alpha-thalassemia trait and hemoglobin H (Hb H) disease.
2) Lipid peroxidation, as measured by plasma TBARS levels, was increased in alpha-thalassemic patients and highest in Hb H patients.
3) Plasma levels of vitamins A, C and E were significantly lower in alpha-thalassemic patients compared to controls. The overall antioxidant capacity correlated inversely with the severity of the alpha-globin gene defect.
This document contains abstracts from the 2005 SWACSM Annual Meeting. The summaries are:
1) A study found that inhibiting ERK1/2 prevented the contraction-induced increase in muscle fatty acid metabolism and CD36 content in a dose-dependent manner, without affecting AMPK activity. ERK1/2 phosphorylation was positively correlated with fatty acid uptake and oxidation. This suggests ERK1/2 is required for contraction-induced increases in fatty acid metabolism.
2) A study found that inhibiting various potassium channels individually did not alter vascular tone in soleus feed arteries, but inhibiting inward rectifier potassium channels and the sodium-potassium pump together significantly increased vascular tone. This suggests these channels contribute to
L'idrossitirosolo è in grado di proteggere e aumentare l'attività dei mitocon...CreAgri Europe
L'idrossitirosolo, un polifenolo estratto dalle olive, attraverso una protezione dei mitocondri, le centrali energetiche della cellula, è in grado di aumentare la resistenza muscolare negli animali da esperimento
Dietary Supplementation with Calcium in Healthy Rats Administered with Artemi...IOSR Journals
Reports on the role of calcium on predisposition to cardiovascular disease have been rather inconsistent while studies on its interaction with other medications are ongoing. We therefore investigated the effect of separate and combine administration of calcium supplement with artemisinin-based combination drug on hepatic and serum lipid profile. Thirty two male wistar rats were randomly assigned into four groups of eight rats each. The control (group A) received normal saline. Group B and D were placed on 10mg/Kg calcium twice daily for four weeks. On the thirtieth day, therapeutic dose of artemisinin-based combination was simultaneously administered to group C and group D twice daily for three days. All the rats were then sacrificed after 12 hours fasting, blood was withdrawn and the liver removed and homogenized in an appropriate buffer. Biochemical analysis showed no significant (p>0.05) variation in hepatic triaacylglycerol in all the treated groups whereas calcium supplementation was observed to induce a significant (p<0.05) reduction in hepatic cholesterol. Significant elevations due to calcium supplementation were also observed in serum total cholesterol, LDL cholesterol level and atherogenic risk index with a concomitant reduction in serum HDL cholesterol. No significant change was observed in serum total cholesterol, triacylglycerol and serum lipoproteins in all other treatment groups. Our study suggests that calcium supplementation may predispose to cardiovascular disease and that its co administration with ACT may not aggravate nor reduced the predisposition risk.
The document discusses DNA repair mechanisms in human lymphocytes. It describes how oxidized purines in DNA are repaired through the base excision repair pathway involving the OGG1 glycosylase. This repair requires activation by the transcription factor NF-YA, which upregulates OGG1 expression when DNA is at risk of oxidation. The repair of oxidized bases by OGG1 in human lymphocytes is slow but can be activated by phytohemagglutinin. Disruption of this global repair of oxidized purines may be due to downregulation of OGG1. A second document discusses how green tea acts as an antioxidant to protect against oxidative stress and influence DNA damage and repair through activation of the Nrf2 transcription factor and upreg
1) The researchers generated mutations of the I-F11 nerve agent bioscavenger enzyme that eliminated glycosylation by mutating asparagine residues 253 and 324.
2) Testing showed the double mutant had increased thermal stability in vitro but no change in circulatory half-life in vivo compared to the glycosylated wild type.
3) Mutation of either residue 253 or 324 alone resulted in partial deglycosylation, while mutation of both residues eliminated glycosylation, indicating these are the sites of glycosylation in the enzyme.
Acute and chronic hyperammonemia modulate antioxidantrkkoiri
1) The study examines the effects of acute and chronic hyperammonemia on antioxidant enzyme levels and oxidative damage in the cerebral cortex and cerebellum of rats.
2) Acute hyperammonemia decreased antioxidant enzyme levels in both brain regions but increased lipid peroxidation only in the cerebellum.
3) Chronic hyperammonemia increased antioxidant enzyme levels in the cerebral cortex but decreased levels in the cerebellum, suggesting the cerebellum is more susceptible to oxidative stress during chronic hyperammonemia.
This document describes a project to develop an in vitro lipoylation reaction to screen chemicals hypothesized to trigger primary biliary cirrhosis (PBC) through molecular mimicry. The student conducted a series of optimization reactions altering amounts of lipoic acid, GTP/ATP, or lipoate activating enzyme and lipoyltransferase. Little success was achieved and time did not allow screening of chemicals, requiring further work. In conclusion, both enzymes are required for lipoylation but varying amounts did not greatly affect the reaction, prompting questions about enzyme purification for future studies.
This document summarizes a study examining the role of platelet-derived growth factor (PDGF)-evoked calcium signaling in human pulmonary fibroblasts. The study found that both acute and overnight PDGF treatment evoked calcium waves in these cells. Blocking external calcium, internal calcium stores, or phospholipase C inhibited the PDGF-evoked calcium waves and PDGF-induced expression of fibronectin and collagen genes. The results indicate that in human pulmonary fibroblasts, PDGF acts through IP3-induced calcium release from internal stores to trigger calcium waves, which then modulate expression of extracellular matrix genes.
Vitamin D plays an important role in regulating calcium and phosphorus balance and promoting bone growth. It also has immunomodulating, anticancer, and neuromodulating effects. The document reviews vitamin D's structure, metabolism, mechanisms of action, and functions in the body. It then discusses recent research on vitamin D's impact on oral cavity diseases, focusing on its potential role in autoimmune conditions of the oral mucosa like recurrent aphthous stomatitis.
This study investigated the effects of inhaling residual oil fly ash (ROFA) particles on oxidative stress and hemodynamics in exercising rats. The key findings were:
1. Rats that inhaled ROFA before exercise showed increased lipid peroxidation (a marker of oxidative stress) in the lungs and heart compared to those that inhaled saline.
2. However, ROFA inhalation did not significantly alter heart rate, blood pressure, or other hemodynamic responses during exercise compared to saline.
3. While ROFA promoted oxidative stress in the lungs and heart, it did not produce significant changes in cardiovascular function during swimming exercise in rats.
It is well established that muscle contraction during exercise lead to elevated levels of reactive oxygen species in skeletal muscle ,hence the growing interest of oxidative stress in exercise
The Role of IL-17, Metaphase Reactants on Patients with Early Rheumatoid Arth...IOSR Journals
Rheumatoid arthritis (RA) represents the most common form of chronic inflammatory joint disease leading to cartilage and bone destruction, It affects approximately 1-2 % of world’s population. The inflammatory process causes diffuse thickening and hyperplasia of the joint Interleukin (IL)-17 is a pleiotropic pro-inflammatory cytokine produced from Th17 cells. IL-17A is the prototypic member belonging to a family of 6 ranging from IL-17A to IL-17F. IL-17A mediates its biological effects through binding to a receptor complex consisting of IL-17RCA and IL-17RCC subunits.
Objective. To assess the role of IL-17 on the disease process of Rheumatoid arthritis and the effects on the trace elements such as Zinc, copper , Magnesium.
Subjects and method: A total of 60 patients with early Rheumatoid Arthritis were studied. Their ages ranged from 20-52 years with a mean age of ( 39.3 ± 9.01)years. , while the range was between (20-51) years and the mean was (37.5 ± 8.6) years for healthy control non significant difference P > 0.4 with no complaint of other chronic or systemic diseases were considered as control. the samples were collected during period from ( December 2012 – july 2013 ). the mean disease duration of RA was (3.27±1.2) month. Blood samples were collected from patients and controls to assess Erythrocyte Sedimentation Rate (ESR) and serum levels of White blood cells ( WBC), Hemoglobin (Hb), Interleukin-17A and were estimated by agglutination test, and IL-17A was estimated by (ELIZA).
Corticosteroid induced disorders – an overviewpharmaindexing
This document provides an overview of corticosteroid-induced disorders and adverse effects. It discusses the mechanism of action of corticosteroids and their effects on muscle, including causing muscle atrophy primarily through increased protein breakdown and inhibition of protein synthesis. It also mentions other corticosteroid-induced disorders like osteoporosis, glaucoma, diabetes, psychosis, and Cushing's syndrome. Prevention of corticosteroid-induced muscle atrophy through growth factors, amino acids, glutamine, taurine, and creatine is also summarized.
The document discusses the immune system and inflammation. It begins with an overview of the cellular components of the immune system, including macrophages, monocytes, basophils, eosinophils, neutrophils, and lymphocytes. It then discusses the process of exercise-induced muscle damage and the subsequent muscle inflammation and regeneration process. This involves leukocyte infiltration, cytokine production, phagocytosis to remove damaged muscle fibers, and satellite cell proliferation to aid regeneration over 1-2 weeks after exercise. NSAIDs are shown to inhibit this inflammatory process and blunt the activity of satellite cells that aid muscle repair.
This document describes a study probing the catalytic mechanism of the insulin receptor kinase (IRK) using a tetrafluorotyrosine-containing peptide substrate. Key findings include:
1) pH-rate profiles indicate the neutral phenol, not the phenoxide ion, is required for phosphorylation, supporting a dissociative transition state.
2) The pKa of the tetrafluorotyrosyl hydroxyl is elevated on the enzyme, and the phenoxide anion behaves as a weak competitive inhibitor.
3) Crystal structures show hydrogen bonding of the tetrafluorotyrosyl group to enzyme residues, consistent with its elevated pKa.
The imbalance between free radical production and endogenous antioxidant defence may result in cellular oxidative stress, causing oxidative damage to various cellular components, such as DNA, proteins and membrane lipids. The human system employs the use of endogenous enzymatic and non-enzymatic antioxidant defence systems against the onslaught of free radicals and oxidative stress.
Unsurprisingly, oxidative damage has been implicated in and is believed to be a key factor causing various pathological conditions, such as cardiovascular disease, neurodegenerative disease, diabetes and cancer. Free radicals can be quenched through a number of mechanisms. Antioxidants directly scavenge free radicals (e.g., via hydrogen atom transfer or electron transfer), prevent free radical formation by chelating metal ions and by interrupting the radical chain reactions of lipid peroxidation, thus retarding its progression. Enzymatic antioxidants include superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase. Non-enzymatic antioxidants include vitamins A, C, and E, glutathione, alpha-lipoic acid, carotenoids, and coenzyme Q10. Other antioxidants include polyphenols, minerals (copper, zinc, manganese, and selenium), and cofactors (B-vitamins). Together, antioxidants work synergistically with each other using different mechanisms against different free radicals and stages of oxidative stress.
The benefits associated with antioxidants are numerous and diverse but it can be a minefield when choosing the appropriate antioxidant support for clients. In this hour-long webinar, Dr Nina Bailey discusses the direct and indirect benefits and actions of key antioxidants including (but not limited to) astaxanthin, alpha lipoic acid, polyphenols and co-enzyme Q10, with a focus on:
-Antioxidant sources and benefits
-Mechanisms and actions
-When to combine antioxidants for synergistic effects
-Overcoming bioavailability issues
-Targeted intervention, which antioxidant(s) and why
The document summarizes the work of the Linus Pauling Institute at Oregon State University over the past 13 years. It discusses the Institute's focus on determining the roles of micronutrients, antioxidants, and other dietary factors in promoting optimal health and preventing disease. Key areas of research include cancer prevention, cardiovascular health, aging, and the protective effects of certain foods/compounds against oxidative stress and inflammation. The Institute has grown from half a researcher in 1996 to 12 full researchers and 4 research assistants currently. The work described emphasizes the important influence of diet and lifestyle on gene expression and chronic disease risk, beyond just genetic factors.
This document summarizes the current state of antifungal drug development and resistance mechanisms. It discusses the classes of antifungal drugs including azoles, polyenes, echinocandins, which act by inhibiting ergosterol biosynthesis or glucan synthesis. Resistance occurs via overexpression of drug targets, efflux pumps, or biofilm formation. Improving drug formulations, combinations, and developing new antifungals are important to address the rise in antifungal infections and resistance.
1) The study examined the effects of diethyl hexyl phthalate (DEHP) exposure on insulin signaling molecules and glucose metabolism in the triceps muscle of rats.
2) Rats treated with DEHP showed increased oxidative stress, reduced insulin receptor and glucose transporter 4 (GLUT4) levels, and decreased glucose uptake and oxidation in the triceps muscle.
3) Co-treatment with antioxidants vitamins C and E prevented the adverse effects of DEHP by reducing oxidative stress, and protecting insulin signaling molecule levels and glucose metabolism in the triceps muscle.
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Glutathione supplementation attenuates
1. ORIGINAL RESEARCH ARTICLE
published: 28 May 2012
doi: 10.3389/fphys.2012.00161
Glutathione supplementation attenuates
lipopolysaccharide-induced mitochondrial dysfunction and
apoptosis in a mouse model of acute lung injury
Saurabh Aggarwal1
, Christiana Dimitropoulou2
, Qing Lu1
, Stephen M. Black1
and Shruti Sharma1
*
1
Vascular Biology Center, Georgia Health Sciences University, Augusta, GA, USA
2
Department of Pharmacology and Toxicology, Georgia Health Sciences University, Augusta, GA, USA
Edited by:
Gregg Rokosh, University of
Louisville, USA
Reviewed by:
Scarlet Y. Shi, Medical College of
Wisconsin, USA
Wenbo Zhang, The University of Texas
Medical Branch, USA
*Correspondence:
Shruti Sharma, Vascular Biology
Center, Georgia Health Sciences
University, 1459 Laney Walker Blvd,
Augusta, GA 30912, USA.
e-mail: shsharma@georgiahealth.edu
Acute lung injury (ALI) is a life threatening condition associated with hypoxemia, diffuse
alveolar damage, inflammation, and loss of lung function. Lipopolysaccharide (LPS; endo-
toxin) from the outer membrane of Gram-negative bacteria is a major virulence factor
involved in the development of ALI. The depletion of glutathione (GSH), an essential intra-
and extra-cellular protective antioxidant, by LPS is an important event that contributes to
the elevation in reactive oxygen species. Whether restoring GSH homeostasis can effec-
tively ameliorate mitochondrial dysfunction and cellular apoptosis in ALI is unknown and
therefore, was the focus of this study. In peripheral lung tissue of LPS-treated mice, hydro-
gen peroxide and protein nitration levels were significantly increased. Pre-treatment with
GSH-ethyl ester (GSH-EE) prevented this increase in oxidative stress. LPS also increased
the lactate/pyruvate ratio, attenuated SOD2 protein levels, and decreased ATP levels in the
mouse lung indicative of mitochondrial dysfunction. Again, GSH-EE treatment preserved
the mitochondrial function. Finally, our studies showed that LPS induced an increase in the
mitochondrial translocation of Bax, caspase 3 activation, and nuclear DNA fragmentation
and these parameters were all prevented with GSH-EE. Thus, this study suggests that
GSH-EE supplementation may reduce the mitochondrial dysfunction associated with ALI.
Keywords: acute lung injury, mitochondrial dysfunction, apoptosis, lipopolysaccharide, glutathione ethyl ester
INTRODUCTION
Acute lung injury (ALI) is an acute inflammatory disorder associ-
ated with both high morbidity and mortality in afflicted patients
even under intensive care. ALI is characterized by non-cardiogenic
dyspnea, hypoxemia, pulmonary neutrophil sequestration, and
low lung compliance (Martinez et al., 2009). ALI can occur in
response to direct insults, such as viral or bacterial infections of
the lung, hyperoxia, and acid aspiration, or indirect insults like
sepsis, multiple transfusions, and pancreatitis. Lipopolysaccha-
ride (LPS) from the outer cell wall of Gram-negative bacteria that
cause sepsis is the most common indirect pulmonary insult lead-
ing to ALI (Erickson et al., 2009). LPS provokes damage to the
alveolar-capillary membrane and the adhesion, activation, and
sequestration of polymorphonuclear neutrophils (PMN), which
result in the deterioration of gas exchange (Nagase et al., 2003).
Although several therapies, such as corticosteroids, prostacyclins,
exogenous surfactants, ketoconazole, and nitric oxide, have shown
promising outcomes, the estimated mortality rate in ALI is 38.5%
(Rubenfeld et al., 2005). Therefore, there is a further need to study
the pathophysiology of ALI and identify therapeutic targets to
improve patient outcome.
Glutathione (GSH), a tripeptide thiol, is the most potent
antioxidant found in the cell and plays a protective role against
reactive oxygen and nitrogen species (ROS and RNS) mediated
injury and lung inflammation (Li et al., 1994; van Klaveren et al.,
1997). This antioxidant is also involved in the regulation of apop-
tosis, cell proliferation, and gene transcription (Rahman and Mac-
Nee, 2000b; Luppi et al., 2005). GSH also modifies protein sulfdryl
groups by a number of reactions: reduction of protein sulfenic
acids, formation of protein mixed disulfides, and their subsequent
reduction. The conjugation of GSH with electrophilic compounds
mediated by glutathione S-transferases (GSTs) and the subsequent
excretion of these conjugates from the cell also serve to protect
against toxins, such as LPS. GSH and GSH-associated enzymes
present in the lower respiratory tract act as the first line of defense
against external agents (DeLeve and Kaplowitz, 1990; Pacht et al.,
1991).Alterations in alveolar and lung GSH metabolism have been
shown to be a central feature of many inflammatory lung diseases,
such as acute respiratory distress syndrome (ARDS), cystic fibro-
sis, and asthma (Rahman and MacNee, 2000a). GSH levels are
significantly reduced in bronchoalveolar lavage fluid (BALF) from
patients with ALI (Pacht et al., 1991). Further, it has been reported
that disruptions in thiol status are associated with ALI (Quinlan
et al., 1994, 1997).
Apoptosis, or programmed cell death, is an essential phys-
iological phenomenon responsible for the selective elimination
of cells. However, the dysregulation of apoptotic signaling path-
ways is thought to play an important role in the development
of ALI (Z’Graggen et al., 2010). Several studies have shown com-
pellingevidencethatincreasedepithelial/endothelialcellapoptosis
www.frontiersin.org May 2012 | Volume 3 | Article 161 | 1
2. Aggarwal et al. Glutathione supplementation and acute lung injury
significantly contributes to the damage to the pulmonary alveolar-
capillary interface in ALI (Kitamura et al., 2001). The inhibition of
apoptosis increases survival in animal models of LPS-induced ALI
(Kawasaki et al., 2000; Ma et al., 2010). Previously, we have shown
that key mechanisms of zinc-mediated apoptotic induction in
pulmonary endothelial cells include the disruption of cellular glu-
tathione homeostasis and decreased mitochondrial ATP synthesis
(Wiseman et al., 2010). We found that the acute increases in both
oxidative and nitrosative stress (similar to that occurring in ALI)
in endothelial cells led to a significant increase in cellular apopto-
sis, which was attenuated by pre-treatment with GSH-ethyl ester
(GSH-EE)(Wisemanetal.,2010).Therefore,thefocusof thisstudy
was to determine whether GSH supplementation can prevent LPS-
mediated ROS/RNS generation, mitochondrial dysfunction, and
cellular apoptosis in mouse model of LPS-induced ALI.
MATERIALS AND METHODS
ANIMAL TREATMENTS
Adult male C57BL/6NHsd mice (7–8 weeks; Harlan, Indianapo-
lis, IN, USA) were used in all experiments and housed in a
temperature-controlledanimalfacilitywith12-hlight–darkcycles.
All animal care and experimental procedures were approved by
the Committee on Animal Use in Research and Education of the
Georgia Health Sciences University (Augusta, GA, USA). Mice
were divided into four experimental groups (n = 6/group); Vehi-
cle, LPS, GSH-EE + LPS, and GSH-EE alone, respectively. Mice
were injected intraperitoneally either with saline (vehicle) or LPS
(6.75 × 104 EU/g body wt), as previously published (Chatterjee
et al., 2008). The group of mice receiving both GSH-EE and LPS
were injected with two separate doses of 2 mmol GSH-EE/kg body
wt (Sigma, St. Louis, MO, USA; Singhal and Jain, 2000). Dose
1 was given 2 h prior to LPS and dose 2 was given along with
LPS injection. The pH of the GSH-EE solution was adjusted to
6.8 by the addition of 2 M NaOH immediately before injection.
Mice belonging to GSH-EE alone group received similar GSH-EE
doses. LPS only and GSH-EE + LPS groups of mice were euth-
anized 12 h after LPS injection, and the lungs were flushed with
ice-cold EDTA-PBS, excised, snap-frozen in liquid nitrogen, and
stored at −80˚C until used.
LUNG TISSUE HOMOGENATES
Lung protein extracts were prepared by homogenizing mouse
lung tissues in Triton lysis buffer (50 mM Tris–HCL, pH 7.6,
0.5% Triton X-100, 20% glycerol) containing a protease inhibitor
cocktail (Sigma). Extracts were then clarified by centrifugation
(14,000 rpm for 10 min at 4˚C). Supernatant fractions were then
assayed for protein concentration using the Bradford reagent (Bio-
Rad, Richmond, CA, USA). Mitochondria were isolated from all
four groups of mice lungs using the Mitochondria Isolation Kit
(Thermo Fisher Scientific Inc., Rockford, IL, USA) and analyzed
by western blot to detect the Bax protein levels.
MEASUREMENT OF GSH LEVELS
Tissue samples were homogenized in 5% sulfosalicylic acid (SSA)
and centrifuged at 12,000 × g for 15 min. Hundred microliters of
supernatant was brought to neutral pH by using triethanolamine
and split into two tubes, one for the total GSH/GSSG and another
to measure the GSSG levels alone (Griffith, 1980). Two microliters
of 2-vinylpyridine (2-VP) was added to the sample tube measuring
GSSG levels alone and placed on a rocker for an hour. 2-VP masks
GSH and prevents its reaction with 5,5 -dithio-bis(2-nitrobenzoic
acid; DTNB), thereby measuring only oxidized GSSG levels. Five
microliters of 50 U/mL glutathione reductase was added to both
the tubes to convert GSSG to GSH. The reaction was then initi-
ated in both tubes by adding, 50 μL of 6 mM DTNB and 350 μL of
0.3 mM NADPH. All solutions were made with 125 mM NaHPO4
buffer (pH 7.5) containing 6.3 mM EDTA. The increase in the
absorbance was measured at 412 nm. Reduced GSH levels were
extrapolated from the GSH/GSSG ratio and the oxidized GSSG
levels and then normalized to tissue wet weight.
IN SITU DETECTION OF H2O2 LEVELS
The Amplex Red Reagent (Molecular Probes) was used to detect
H2O2 levels in fresh lung tissue samples obtained from mice
treated with LPS in the presence or absence of GSH-EE supple-
mentation. Briefly, an equal amount (∼10 mg/sample) of lung
tissuewasincubatedat37˚Cfor30 mininmastermixsolutioncon-
taining Amplex Red reagent, horseradish peroxidase, and a buffer
solution. Supernatant was then collected, fluorescence was read
at excitation/emission 530/590 nm, and concentrations of H2O2
were determined through extrapolation from a standard curve.
Protein in the tissue samples was estimated by BCA assay and used
to normalize the detected H2O2 levels.
MEASUREMENT OF PROTEIN NITRATION
The total nitrated protein levels were measured in the lung
homogenates of mice treated with LPS in the presence or absence
of GSH-EE via a dot blot procedure. Briefly, 30 μg protein
was applied to a nitrocellulose membrane pre-soaked with Tris-
buffered saline (TBS). After the protein samples were completely
transferred,the membrane was blocked in 5% fat-free milk for 1 h,
washed with TBS, and incubated with mouse anti-3-nitrotyrosine
(1:100, Calbiochem) antibody overnight. Finally, the membrane
was incubated with goat anti-mouse IgG for 2 h. The reactive dots
were visualized using chemiluminescence (Pierce Laboratories) on
a Kodak 440CF image station (New Haven, CT, USA). The band
intensity was quantified using Kodak 1D image processing soft-
ware. The protein expression was normalized by re-probing with
mouse anti β-actin antibody.
WESTERN BLOT ANALYSES
The lung tissue sample homogenates containing 25–50 μg pro-
teins were separated on 4–20% denaturing polyacrylamide gels
and transferred to Immunoblot-PVDF membranes (Bio-Rad Lab,
Hercules, CA, USA). The membranes were blocked with 5%
non-fat dry milk in TBS containing 0.1% Tween (TBST). After
blocking, each membrane was incubated overnight at 4˚C with
either SOD1 (1:500, custom-made), SOD2 (1:1000, LS Biochem-
icals), Bax (1:1000, Cell Signaling, Danvers, MA, USA), cleaved
caspase 3 (1:1000, Cell Signaling, Danvers, MA, USA), cleaved
caspase 7 (1:1000, Cell Signaling, Danvers, MA, USA), Voltage-
dependent anion channels (VDAC; 1:500, Cell Signaling, Danvers,
MA, USA), or mouse β-actin (1:5000, Sigma), washed with TBST,
and then incubated with a IgG-horseradish peroxidase. The reac-
tive bands were visualized using chemiluminescence (SuperSignal
Frontiers in Physiology | Oxidant Physiology May 2012 | Volume 3 | Article 161 | 2
3. Aggarwal et al. Glutathione supplementation and acute lung injury
West Femto substrate kit; Pierce) on a Kodak 440CF image sta-
tion. The band intensity was quantified using Kodak 1D image
processing software. To normalize for equal protein loading, the
blots were re-probed with either β-actin or VDAC.
DETERMINATION OF LACTATE AND PYRUVATE LEVELS
The lung tissues were homogenized in ice-cold 0.5 M perchlo-
ric acid and centrifuged at 14,000 rpm for 20 min. The collected
supernatants were then neutralized with 3 M KHCO3 and used
for the lactate and pyruvate assays. The relative changes in lac-
tate levels were measured using a lactate assay kit (BioVision).
Thepyruvatelevelsweredeterminedusingthespectrophotometric
enzymatic measurement assay at 340 nm, as previously published
(Sharma et al., 2008). NADH was used as a cofactor and lactate
dehydrogenase as the coenzyme.
ATP DETERMINATION
The ATP levels were quantified with a commercially available kit
(Invitrogen) based on the firefly luciferin-luciferase reaction. In
this reaction, ATP is consumed and light is emitted when firefly
luciferase catalyzes the oxidation of luciferin. The amount of light
emitted during the reaction is proportional to the availability of
ATP. The luminescence was determined using a Fluoroscan Ascent
plate luminometer (Thermo Electron, Corp.), and the ATP levels
reported in nanomoles per milligram protein.
MEASUREMENT OF CASPASE 3/7 ACTIVITY
Caspase 3/7 activity was detected with the caspase-Glo 3/7 assay
kit (Promega Corporation, Australia). Briefly, 100 μL caspase 3/7
reagents were added to each well with 50 μL peripheral lung
homogenates containing equal amount of protein and incubated
for 1 h on rotary shaker at room temperature. The lumines-
cence intensity was measured using a Multiskan Microplate reader
according to the manufacturer’s instructions.
DETERMINATION OF LUNG APOPTOSIS
The lung tissues were washed in PBS, fixed in 4% paraformalde-
hyde (RT, 1 h), then placed in 30% sucrose (RT, 1 h), embedded
in O.C.T embedding medium (Tissue-Tek, Sakura Finetechnical,
Tokyo, Japan), and stored at −80˚C overnight. The embedded
tissue blocks were sectioned to 5 μm slices and were analyzed
for the presence of apoptotic nuclei using the DeadEnd Fluoro-
metric TUNEL System (Promega, Madison, WI, USA). The DNA
breaks were labeled with fluorescein-12-dUTP (green), and the
nuclei were stained with propidium iodide (red). The vascular
endothelial cell nuclei were labeled with anti-Von Willebrand Fac-
tor (1:500; Abcam, Cambridge, MA, USA), and Alexa Fluor 350
(blue) was used as the secondary antibody (Invitrogen, Carls-
bad, CA, USA). The sections were examined under a fluorescence
microscope (Olympus,Japan).All images were captured by Image-
Pro Plus ver 5.0 (Media Cybernetics, Silver Spring, MD, USA).
The quantification of the TUNEL positive endothelia nuclei and
total endothelia nuclei was processed by Image-Pro software and
presented as a percentage.
STATISTICAL ANALYSIS
The experimental results are expressed as mean ± standard error
of the mean (SEM) for n = 5–6 for each group. The statistical
analysis was done using GraphPad Prism version 4.01 forWindows
(GraphPad Software, San Diego, CA, USA) using one-way analysis
of variance (ANOVA) and a Newman–Keuls multiple comparison
post hoc test. A value of P < 0.05 was considered significant.
RESULTS
GSH-EE SUPPLEMENTATION ATTENUATES LPS-INDUCED OXIDATIVE
AND NITROSATIVE STRESS IN THE MOUSE LUNGS
To determine the effect of LPS exposure on oxidant and antiox-
idant homeostasis, we first measured the glutathione levels in
the peripheral lung tissue of mice treated with or without LPS.
There was a significant (approximately threefold) reduction in
GSH levels in LPS-treated mouse lung when compared to controls
(Figure 1A) that was preserved with GSH-EE supplementation.
Earlier studies have provided evidence indicating that ROS and
RNS, particularly H2O2 and ONOO−, contributes to the patho-
genesis of ALI (Lang et al., 2002; Tasaka et al., 2008). Therefore,
we determined whether GSH-EE pre-treatment attenuated LPS-
induced oxidative and nitrosative stress in the mouse lung mice.
We found elevated H2O2 levels (Figure 1B) and increased pro-
tein nitration (Figure 1C). GSH-EE pre-treatment preserved the
LPS-induced H2O2, and nitrated protein levels in the mouse lung
(Figure 1).
GSH-EE SUPPLEMENTATION PRESERVES MITOCHONDRIAL FUNCTION
The loss of mitochondrial function upon exposure to LPS has been
implicated in the development of ALI (Kuwabara and Imajoh-
Ohmi, 2004). Superoxide dismutase-1 and 2 (SOD1 and SOD2)
are the two important antioxidant enzymes actively involved in
quenching harmful superoxide radicals. The lungs of LPS-treated
mice had a approximately twofold decrease in SOD1 and SOD2
protein levels (Figures 2A,B), while pre-treatment with GSH-EE
preserved both SOD1 and SOD2 protein levels. Under physiologi-
cal conditions where mitochondrial function is optimal, pyruvate
levels are higher than lactate, and thus any increase in lactate levels
will decrease the pyruvate-to-lactate ratio and can be extrapolated
to suggest a switch in mitochondrial ATP generation from glu-
coneogenesis to glycolysis (Shinde et al., 2005). The LPS-exposed
mice had a significantly higher lactate/pyruvate ratio, thus indi-
cating a disruption in lung mitochondrial ATP generation from
gluconeogenesis (Figure 2C) and this was confirmed by an overall
decrease in ATP levels in the LPS-treated mouse lung (Figure 2D).
Pre-treatment with GSH-EE blocked the increase in the lac-
tate/pyruvate ratio (Figure 2C) and preserved ATP generation
(Figure 2D).
GSH-EE SUPPLEMENTATION PREVENTS THE LPS-INDUCED
MITOCHONDRIAL TRANSLOCATION OF Bax AND CASPASE 3/7
ACTIVATION
Bax,a member of the Bcl-2 family of proteins,promotes apoptosis.
The translocation of Bax from the cytoplasm to the outer mito-
chondrial membrane stimulates apoptosis (Sarkar et al.,2003). We
found higher Bax protein levels in the mitochondria isolated from
the LPS-treated mice compared to the control mice (Figure 3A).
GSH-EE prevented the LPS-induced mitochondrial translocation
of Bax (Figure 3A). Activated caspases 3 and 7 are the execu-
tors of apoptotic cell death. Western blot analysis demonstrated a
www.frontiersin.org May 2012 | Volume 3 | Article 161 | 3
4. Aggarwal et al. Glutathione supplementation and acute lung injury
FIGURE 1 | GSH-EE supplementation improves LPS-mediated decrease in
GSH levels and attenuates LPS-induced oxidative and nitrosative stress.
Lungs from vehicle, LPS, GSH-EE + LPS, and GSH-EE treated mice were used
for the analysis of GSH, H2O2, and nitrated proteins levels. LPS significantly
decreased GSH levels but GSH-EE pre-treatment prevented the loss of GSH
in these animals (A).The Amplex Red assay measurement indicated a twofold
increase in H2O2 levels in LPS-treated mice lungs as compared to controls
(B). Total nitrated protein levels were measured by dot blot analysis (C). LPS
caused significant increases in the formation of H2O2 and nitrated proteins,
whereas GSH-EE pre-treatment prevented the LPS-induced increase in these
oxidative and nitrosative stress parameters. Values are mean ± SEM;
n = 6/group. *P < 0.05 vs. vehicle;
†
P < 0.05 vs. LPS alone.
twofold increase in activated caspase 3 (Figure 3B), and a three-
fold increase in activated caspase 7 (Figure 3C),protein levels after
LPS exposure. However, pre-treatment with GSH-EE significantly
reduced only activated caspase 3,and not activated caspase 7,levels
(Figures 3B,C). Furthermore, using a luminescent assay, we found
a fivefold increase in caspase 3/7 activity in LPS-treated mice, as
compared to the controls (Figure 3D). GSH-EE pre-treatment
partially decreased caspase 3/7 activity (Figure 3D). These results
suggest that GSH-EE pre-treatment attenuated the LPS-mediated
increase in caspase 3/7 activity primarily by decreasing active
caspase 3 levels.
GSH-EE SUPPLEMENTATION PROTECTS AGAINST LPS-INDUCED
APOPTOSIS
TUNEL staining was carried out to determine the effect
of GSH-EE pre-treatment on apoptosis in the LPS-exposed
mouse lungs. Sections were co-stained with TUNEL, a nuclei
marker (propidium iodide), and endothelial cell marker (VWF;
Figure 4A). The quantitation of TUNEL positive cells indicated
increased endothelial cell apoptosis in LPS-treated mice lungs,
which was attenuated with GSH-EE administration (Figure 4B).
In animals receiving vehicle or GSH-EE alone, no apoptotic nuclei
were observed (Figure 4).
DISCUSSION
The data presented in this study demonstrate that GSH-EE supple-
mentation can protect the mouse lung against LPS-induced mito-
chondrial dysfunction and subsequent endothelial cell apoptosis.
The prior administration of GSH-EE: preserved LPS-mediated
attenuation of GSH levels; reduced the LPS-induced oxidative
and nitrosative stress; mitigated the LPS-mediated disruption of
mitochondrial ATP generation; prevented the LPS-induced loss of
Frontiers in Physiology | Oxidant Physiology May 2012 | Volume 3 | Article 161 | 4
5. Aggarwal et al. Glutathione supplementation and acute lung injury
FIGURE 2 | GSH-EE supplementation prevents LPS-mediated
mitochondrial dysfunction. Protein extracts prepared from peripheral lungs
of vehicle, LPS, GSH-EE + LPS, and GSH-EE treated mice were analyzed by
Western blot analysis using a specific antiserum raised against SOD1 (A) or
SOD2 (B). Protein levels were normalized for loading using β-actin. A
representative blot and normalized densitometric values are shown. There
was a significant decrease in SOD1 and SOD2 protein levels in LPS-treated
mice and this was prevented by GHS-EE pre-treatment. The lactate/pyruvate
ratio was also determined in all four groups (C). LPS-exposed mice had a
significantly higher lactate/pyruvate ratio (C). Pre-treatment with GSH-EE
preserved the lactate/pyruvate ratio. There was a significant reduction in lung
ATP levels after LPS exposure (D). However, the LPS-mediated decrease in
ATP levels was not observed in mice pre-treated with GSH-EE (D). Values are
mean ± SEM; n = 6/group. *P < 0.05 vs. vehicle;
†
P < 0.05 vs. LPS.
the mitochondrial antioxidant enzyme SOD2; decreased the LPS-
mediated mitochondrial translocation of Bax,caspase 3 activation;
and prevented endothelial apoptosis.
GSH has been shown to protect cells against toxins, ROS,
and RNS (Messana et al., 1988). The depletion of GSH is an
important pathological event in the development of many neu-
rological, hepatic, and pulmonary diseases (Lee et al., 2004; Var-
gas et al., 2011). Although the cause of GSH depletion is not
clear, traditionally, it is thought that the decrease in GSH lev-
els is a direct consequence of increased production of ROS and
RNS. For example, a common pathological hallmark in var-
ious neurodegenerative disorders, such as amyotrophic lateral
sclerosis, Alzheimer’s, and Parkinson’s diseases, is the increase
in oxidative stress and a subsequent decrease in the GSH con-
tent. The intraperitoneal administration of the GSH precursor,
N-acetylcysteine (NAC), to rats has been shown to replen-
ish GSH levels and provide protection against oxidative dam-
age to brain proteins in these neurodegenerative disorders
(Pocernich et al., 2001). In addition, drugs, infections, and
inflammation in the liver increase the generation of ROS
and RNS with concomitant decreases in GSH levels. Liver
injury induced by acetaminophen also involves the severe
depletion of both cytosolic and mitochondrial GSH pools,
increased ROS generation, and cell death. NAC has been
proven to be effective in treating acetaminophen and non-
acetaminophen induced liver injury that, conversely, is poten-
tiated by GSH depletors (Jaeschke and Bajt, 2006; Lee et al.,
2009). In human studies,high-dose of NAC significantly improved
the antioxidant profile of the lungs by elevating GSH lev-
els and elicited an antioxidant effect at the alveolar surface
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6. Aggarwal et al. Glutathione supplementation and acute lung injury
FIGURE 3 | GSH supplementation prevents the LPS-mediated
translocation of Bax and caspase 3 activation. Bax protein levels were
measured in the mitochondria isolated from lung homogenates of vehicle,
LPS, LPS + GSH-EE, and GSH-EE treated mice using Western blot analysis
(A). Mitochondrial Bax protein levels from LPS-treated mouse lungs were
significantly higher and GSH-EE prevented this translocation (A). Activated
caspase 3 and 7 protein levels and activity were also determined in lung
homogenates from all four groups. LPS-treated mice had significantly
increased activated caspase 3 protein levels that was reduced by GSH-EE
pre-treatment (B). The activated caspase 7 protein levels were also
upregulated in LPS-treated mice, but did not change after GSH-EE
pre-treatment (C). The increase in activated caspase 3 protein levels
correlated with an increase in caspase 3/7 activity in lungs of mice
exposed to LPS, which was significantly decreased in presence of GSH-EE
(D). Values are mean ± SEM; n = 3–6/group. *P < 0.05 vs. vehicle;
†
P < 0.05 vs. LPS.
in patients with fibrosing alveolitis. These biochemical changes
were accompanied by an improvement in the pulmonary func-
tion tests of these patients (Behr et al., 1997). In placebo-
controlled trials, orally administered NAC (≥600 mg/day) has
been shown to significantly improve the response to steroids,
increase the general well-being, and decrease exacerbation rates,
emergency room visits, days of illness, costs of hospitaliza-
tion, and work time loss in patients with chronic lung diseases
(Grandjean et al., 2000; Kasielski and Nowak, 2001; Poole and
Black, 2001).
In recent years, several studies have delineated the role of GSH
depletion and subsequent increase in oxidative stress and cytokine
Frontiers in Physiology | Oxidant Physiology May 2012 | Volume 3 | Article 161 | 6
7. Aggarwal et al. Glutathione supplementation and acute lung injury
FIGURE 4 | GSH supplementation inhibits the LPS-induced endothelial
cell apoptosis in the mouse lung. Lung tissue sections from vehicle, LPS,
LPS + GSH-EE, and GSH-EE treated mice were analyzed for the presence
of apoptotic nuclei using the DeadEnd Fluorometric TUNEL System. The
DNA breaks were labeled with fluorescein-12-dUTP (TUNEL; green), and
the nuclei were stained using propidium iodide (PI; red). Vascular
endothelial cells were labeled with anti-Von Willebrand Factor (VWF; blue)
(A). The quantification of the TUNEL positive endothelial nuclei and total
endothelial nuclei was processed by Image-Pro software and presented as
a percentage. LPS treatment caused a significant increase in endothelial
apoptosis and this was attenuated by GSH-EE pre-treatment (B). Values are
mean ± SEM; n = 4/group. *P < 0.05 vs. vehicle;
†
P < 0.05 vs. LPS.
production in the development of ALI. Although, the role of GSH
in the LPS-induced pulmonary inflammation may be cell specific.
For example, in murine macrophage-like cells (RAW 264.7), LPS
elevated GSH levels but when these LPS-treated cells were supple-
mented with GSH precursor, NAC, there was attenuation in the
LPS-dependent inflammation. However, the co-administration of
NAC with the anti-inflammatory agent, ethyl pyruvate, partially
reversed the anti-inflammatory effects of ethyl pyruvate suggest-
ing a dual role of GSH in these murine macrophage-like cells
(Song et al., 2004). In alveolar type II epithelial cells of rat lungs,
LPS decreased GSH, which resulted in an increase in TNF-α and
nuclear factor (NF)-kappa B levels (Zhang et al., 2008). Further-
more, the inhibition of GSH biosynthesis by buthionine sulfox-
imine (BSO) increased intracellular oxidative stress and enhanced
the production of IL-1β, IL-6, and TNF-α in the alveolar epithe-
lium (Haddad et al., 2001). In polymicrobial sepsis induced by
cecal ligation and puncture, exogenous GSH protected mice from
the lethal quantities of ROS and RNS produced by neutrophils. In
these mice, GSH stimulated the migration of neutrophils to the
site of infection but prevented their infiltration to distant sites,
thereby promoting the bactericidal, while limiting the cytolytic,
properties of neutrophils (Villa et al., 2002). Interestingly, tempol,
a membrane permeable free radical scavenger, ameliorated LPS-
induced GSH depletion in a mouse model of ALI (El-Sayed et al.,
2011),suggesting that oxidants potentiate the cellular reduction of
GSH levels. In support of these studies, our present data demon-
strated that LPS exposure significantly diminished GSH levels in
the mouse lung, which correlated with an increase in pulmonary
oxidative and nitrosative stress. Exogenous supplementation with
GSH-EE in the presence of LPS restored the GSH levels and subse-
quently attenuated the LPS-induced elevation of both H2O2 and
total protein nitration in the mouse lung. We also found that
LPS-challenge led to the attenuation of SOD1 and SOD2 pro-
tein levels, whereas this decrease was not observed in GSH-EE
pre-treated mice. Although it is unclear how LPS treatment led to
the down-regulation of these enzymes in the mouse lung, based
upon previous studies, we can speculate that the activation of pro-
tein kinase B (PKB)/Akt by LPS and also by H2O2 leads to the
down-regulation of SOD levels (Wang et al., 2000; Monick et al.,
2001). Akt has been shown to phosphorylate and repress the DNA
binding activity of the transcription factor, FOXO3a (Forkhead
box class O), and consequently decrease the gene expression of
SOD2 (Li et al., 2006).
Intracellular redox status regulates various aspects of cellu-
lar function. Mitochondrial function is particularly susceptible to
oxidative damage. For example, unopposed oxidative stress selec-
tively inhibits mitochondrial respiratory-chain enzymes, thereby
decreasing ATP synthesis (Drahota et al., 2005). Studies in Parkin-
son’s and Alzheimer’s diseases have shown that if ONOO− is not
scavenged, e.g., by antioxidants, such as reduced GSH, it leads to
irreversible damage to critical cellular enzymes of the mitochon-
drial electron transport chain, alpha ketoglutarate dehydrogenase,
and pyruvate dehydrogenase (Foxton et al., 2007). Biochemically,
LPS increases the NADH:NAD ratio, thereby shifting the equi-
librium of the lactate dehydrogenase reaction toward lactate and
causing profound lactic acidosis. The resultant increase in the lac-
tate/pyruvate ratio is characteristic of attenuated oxidative phos-
phorylation in the mitochondria. Interestingly, our data demon-
strate that mice supplemented with GSH-EE prior to LPS exposure
are protected against the LPS-dependent increase in the lac-
tate/pyruvate ratio and maintain normal ATP levels. These results
compliment other studies that have shown that GSH protects the
mitochondria against oxidative insult under various pathologic
conditions such as reperfusion injury in the liver, ethanol-induced
toxicity, and diabetes (Sommer et al., 2012).
Mitochondrial regulation of apoptosis is central to cell survival.
A recent study demonstrated that LPS-mediated cellular apop-
tosis is dependent upon mitochondrial dysfunction in human
monoblastic U937 cells (Kuwabara and Imajoh-Ohmi, 2004).
Mitochondria regulate apoptosis by maintaining a delicate bal-
ance between pro- and anti-apoptotic Bcl-2 proteins. The pro-
apoptotic member of the Bcl-2 protein family, Bax, is normally
found in the cytosol where it acts as a sensor for cellular stress.
LPS-induced oxidative and nitrosative stress activates Bax, which
www.frontiersin.org May 2012 | Volume 3 | Article 161 | 7
8. Aggarwal et al. Glutathione supplementation and acute lung injury
then translocates to the outer mitochondrial membrane where it
interacts with anti-apoptotic proteins (Mishra and Dhali, 2007).
This interaction between Bax and anti-apoptotic proteins disrupts
their normal function,which leads to the formation of pores in the
mitochondria, allowing the exit of cytochrome c. In addition, ROS
independently oxidize mitochondrial pores further contributing
to cytochrome c release upon GSH depletion (Costantini et al.,
1996). Cytochrome c released from the mitochondria activates the
caspase cascade,which then induces apoptosis. Recent studies have
shown that LPS triggers the release of cytochrome c from the mito-
chondria to the cytoplasm, thereby activating the caspase cascade
and DNA fragmentation (Chuang et al., 2011). The pre-treatment
of human alveolar epithelial A549 cells with NAC significantly
decreasedLPS-mediatedcaspaseactivationandDNAdamage,sug-
gesting an important role of GSH in ameliorating LPS-induced
cell death (Chuang et al., 2011). The loss of GSH and subsequent
decrease in ATP and ensuing cell death by dopamine metabolites
is prevented by GSH-EE and NAC supplementation, suggesting
a critical role of GSH in maintaining functional mitochondria in
Parkinson’s disease (Nunes et al., 2011). GSH also exhibited anti-
apoptotic effects in selenium induced apoptosis in HSC-3 human
oral squamous cell carcinoma cells and arsenic trioxide-induced
apoptosis in lymphoma cells (Takahashi et al., 2005). Thus, our
findings in the mouse model of ALI concur with these studies and
demonstrate that GSH-EE pre-treatment prevents LPS-induced
pulmonary endothelial cell apoptosis.
In conclusion, the results presented in this study suggest that
the modulation of the cellular redox equilibrium by GSH pre-
serves oxidative phosphorylation in the mitochondria in response
to LPS-induced oxidative damage and subsequent cellular apopto-
sis. Although clinical trials involving the use of antioxidants have
not shown promising results in chronically ill patients, the acute
burst of oxidative, and nitrosative stress seen in ALI may be more
susceptible to antioxidant therapy. Based upon past studies and
our present data, we conclude that GSH depletion plays a vital role
in the pathogenesis of ALI, and therefore, GSH supplementation
may act as an important adjuvant therapy to reverse the disease
process.
ACKNOWLEDGMENTS
The authors thank Imran Rehmani, Suphin Kallarackal, Johnny
Wright, and Sumant Ponnala for their excellent technical assis-
tance. This research was supported in part by a Beginning Grant in
Aid(09BGIA2310050)fromtheSoutheastAffiliates,andaScientist
Development Grant (11SDG7460024; all to Shruti Sharma) from
the American Heart Association; 1P01HL101902-01A, HL60190,
HL67841, and HL101902 (all to Stephen M. Black) from the
National Institutes of Health.
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