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Formal Lab Report
7/8 Grades
Ashbrook Independent School
Explore the concept of variation
Follow three generations of a species
Cross homozygous dominant and recessive
parents
Cross F1 generation
Find ratios in F1 and F2 generations
Easy to use indoors
Uses small amount of space
Grows quickly
Distinctive traits
Ideal for Mendelian genetics
Lighting system




Quads with plants
Grow seeds on paper towels in petri dishes.
Grow F1 first, then grow F2.
Keep petri dishes in 2 cm of water.
Allow growth for 4-5 days.
Count the purple and green stems.


             Petri dishes with seedlings.
Count leaf hairs on margin of first true leaf
Collect class data
Create a histogram of hair variation
Also, record parent stem colors




                             Leaf hairs on margin
Parent Stem Colors

             P1                          P2
             Generation                  Generation

Stem Color   No. Plants    Class         No. Plants   Class
                           Average                    Average
Non-purple
(anl)
Purple
(ANL)
Ratio
ANL:anl
F1 and F2 Stem Colors

             F1 Generation                   F2 Generation

Color Stem   No. Plants      Class Average   No. Plants      Class Average

Non-purple
anl
Purple
ANL
Ratio
ANL:anl
Number of Leaf Hairs on P1
                   7



                   6



                   5
Number of Plants




                   4



                   3



                   2



                   1



                   0
                       0   1   2   3   4   5   6   7   8   9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29
                                                                   Number of Leaf Hairs
Parents             A (ANL)   A (ANL)
a (anl)             Aa        Aa
a (anl)             Aa        Aa



F1 Genotype = Aa
F1 Phenotype = purple stem

Note: ANL= anthocyanin
      anl=anthocyaninless
F1 Parents   A          a
    A            AA         Aa
    a            Aa         aa




F2 Genotype = 1:2:1 = AA:Aa:aa

F2 Phenotype = 3 purple stem : 1 green stem
After examining the first generation parents and
their offspring, what can you conclude about
the second generation (F1)?
What are the dominant and recessive stem
colors?
How did you determine which stem color trait
was dominant?
Did this experiment demonstrate Mendel’s 3:1
ratio? Which generation showed this ratio?
 How did the F2 generation compare to the
original parent generation?
Describe the variation in plant hairs.

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Genetics of wisconsin fast plants

  • 1. Formal Lab Report 7/8 Grades Ashbrook Independent School
  • 2. Explore the concept of variation Follow three generations of a species Cross homozygous dominant and recessive parents Cross F1 generation Find ratios in F1 and F2 generations
  • 3. Easy to use indoors Uses small amount of space Grows quickly Distinctive traits Ideal for Mendelian genetics
  • 5. Grow seeds on paper towels in petri dishes. Grow F1 first, then grow F2. Keep petri dishes in 2 cm of water. Allow growth for 4-5 days. Count the purple and green stems. Petri dishes with seedlings.
  • 6. Count leaf hairs on margin of first true leaf Collect class data Create a histogram of hair variation Also, record parent stem colors Leaf hairs on margin
  • 7. Parent Stem Colors P1 P2 Generation Generation Stem Color No. Plants Class No. Plants Class Average Average Non-purple (anl) Purple (ANL) Ratio ANL:anl
  • 8. F1 and F2 Stem Colors F1 Generation F2 Generation Color Stem No. Plants Class Average No. Plants Class Average Non-purple anl Purple ANL Ratio ANL:anl
  • 9. Number of Leaf Hairs on P1 7 6 5 Number of Plants 4 3 2 1 0 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 Number of Leaf Hairs
  • 10. Parents A (ANL) A (ANL) a (anl) Aa Aa a (anl) Aa Aa F1 Genotype = Aa F1 Phenotype = purple stem Note: ANL= anthocyanin anl=anthocyaninless
  • 11. F1 Parents A a A AA Aa a Aa aa F2 Genotype = 1:2:1 = AA:Aa:aa F2 Phenotype = 3 purple stem : 1 green stem
  • 12. After examining the first generation parents and their offspring, what can you conclude about the second generation (F1)? What are the dominant and recessive stem colors? How did you determine which stem color trait was dominant? Did this experiment demonstrate Mendel’s 3:1 ratio? Which generation showed this ratio? How did the F2 generation compare to the original parent generation? Describe the variation in plant hairs.