(1) The study estimated the above-ground biomass of Milanchowk Community Forest in Nepal using field inventory data and satellite imagery. (2) A regression model was developed between above-ground biomass measured in 20 sample plots and vegetation indices derived from a Sentinel 2 image. (3) The maximum, minimum and average biomass estimates from the sample plots were 2164 kg, 422 kg and 1263 kg respectively. The regression model was validated and found to accurately estimate biomass across 10 additional plots.