Tropical tree species identification is critical for studies of forest habitat, composition, biomass, and determining the role of forests in climate variability through carbon uptake. The aim of the research was to derive an accurate classification of a tropical forest study site in Costa Rica using high-resolution imagery. A series of corrections for look and view angle, and the utilization of the DigitalGlobe atmospheric compensation procedure (AComp) provided the study with an accurate surface reflectivity dataset from WorldView-3 imagery. A rule-set object-oriented classification schema defined trees in the study area using ENVI-defined tree canopies through a segmentation of the multispectral image. The results show that select WorldView-3 bands, and WorldView-3-specific vegetation indices, can produce an accurate species-level tree classification in a complex tropical forest.