1. The biological basis for predicting nitrogen and sulfur isotope ratio discrimination in animals is complicated by mixed diets of varying protein quality and quantity consumed by omnivores.
2. The study examines how protein quality, defined as the relative amount of the most limiting amino acid, and protein quantity determine nitrogen (D15N) and sulfur (D34S) isotope discrimination in mammals and birds. Low protein quality and high protein content have the potential to increase D15N by increasing protein turnover.
3. Protein quality accounted for most variation in D15N for diets of intermediate protein quality and quantity, but diets with exceptionally high or low protein quality showed different discrimination than predicted by quality alone. Supplementation
Effect of Replacement of Sweet Orange (Citrus Sinensis) Peel Meal with Maize ...IOSR Journals
This document summarizes a study that evaluated the effect of replacing maize offal with graded levels of sweet orange peel meal (SOPM) in diets for West African Dwarf goats. Sixteen goats were fed one of four diets containing 0%, 12.5%, 25%, or 50% SOPM for 84 days. Results showed no significant differences in nutrient digestibility or intake among the diets, except for ether extract intake which was lower for the 50% SOPM diet. The study concluded that SOPM can replace up to 50% of maize offal in goat diets without negatively impacting nutrient digestibility or intake.
This document describes a computer model developed to predict the responses of lactating ewes to concentrate supplementation. The model considers the effects of concentrate supplementation timing and levels on ruminal pH and forage intake. It simulates organic acid production from carbohydrate fermentation, saliva buffer production, and ruminal pH based on these factors. The model was able to predict the effects of increasing concentrate supplementation levels and timing on dry matter intake and forage substitution. Simulations suggested feeding concentrate in a single evening meal or in no more than two meals per day to minimize negative impacts on forage intake.
This study used Illumina sequencing of the 16S rRNA gene to analyze changes in the rumen microbiome of cattle subjected to feed restriction. The main findings were:
1) Feed restriction caused a large decrease in an abundant Succinivibrionaceae species and a significant increase in Methanobrevibacter gottschalkii.
2) Fifty-five days after being returned to a normal diet, the rumen microbiomes of previously restricted cattle were not different from controls, showing the disruption from restriction did not have long-term effects.
3) The rumen microbiome was not associated with compensatory growth in cattle 55 days after restriction ended.
metabolic acceleration and the evolution of human brainJoão Soares
Humans have larger brains, higher reproductive output, and longer lifespans than other great apes, presenting an "energetic paradox" that requires more energy expenditure. This study found that:
1) Humans have higher total daily energy expenditure (TEE) than chimpanzees, bonobos, gorillas and orangutans, even after controlling for factors like body size and physical activity.
2) Much of the increased human TEE is due to a higher basal metabolic rate (BMR), indicating increased metabolic activity of organs.
3) Humans also have a greater percentage of body fat than other great apes.
The findings suggest humans evolved an "accelerated
This document discusses amino acid interactions, specifically amino acid imbalances and antagonisms. It begins by defining imbalances and antagonisms based on Harper's 1956 definitions. Imbalances occur when supplementing all essential amino acids except the first limiting one, while antagonisms involve a structurally related amino acid increasing the requirement for another. The document then discusses several examples of imbalances and antagonisms, how they impact growth and amino acid requirements. It reviews research showing imbalances do not generally increase catabolism of the limiting amino acid, but antagonisms can. The document also discusses how imbalances and antagonisms depress food intake in animals given a choice.
Loss of the RNA polymerase III repressor Maf1 confers obesity resistanceAsh Byrnes
1) Mice with whole-body knockout of the RNA polymerase III repressor MAF1 are resistant to diet-induced obesity and nonalcoholic fatty liver disease. They have reduced food intake and increased metabolic inefficiency.
2) Energy expenditure is increased in MAF1 knockout mice through multiple mechanisms, including increased precursor tRNA synthesis and futile cycling of tRNAs and hepatic lipids.
3) Metabolite profiling reveals elevated levels of amino acids and spermidine in MAF1 knockout mice, linking induction of autophagy to their extended lifespan. Increased spermidine and reduced nicotinamide N-methyltransferase are associated with obesity resistance.
This study assessed the effects of minerals calcium, magnesium, and inorganic phosphorus on cyclicity in Nili Ravi buffaloes. 90 buffaloes were divided into cyclic (n=30), non-cyclic (n=30), and repeat breeders (n=30) groups. Blood samples were tested and calcium was significantly higher in cyclic buffaloes than non-cyclic and repeat breeders. Magnesium levels were similar in cyclic and non-cyclic buffaloes but significantly higher in repeat breeders. Inorganic phosphorus was imbalanced in non-cyclic and repeat breeders. The study concluded calcium and phosphorus should be in a 2:1 ratio and imbalances may result in infertility
Achieving factual sustainability in fish farming needs the addition of most of the fish meal exploited as feed stuffs. The current experiment described two feeding trials, that resulted in the complete replacement of fish meal in the fingerling of Tilapia mossambicus. The initial trial was accompanied with three stages of fish meal replacement (50, 75 and 100% of dietary protein) viz., one level of soy protein and two levels of Lactat ® Probiotic (0 (or) 0.3% of the diet). Since probiotic has been reported to promote gut health, it was incorporated inorder to examine the growth enhancement and whether it would ease high levels of fish meal replacement in T. mossambicus. Lipids were provided by Cod liver oil. The better weight gain was observed in the treatments 50/50+ of fish meal replacement and 0/100+ of fish meal replacement. The optimum Specific Growth Rate, Food Conversion Ratio and Survival were also observed in 50/50+ fish meal replacement and 0/100+ fish meal replacement. The higher serum Acetyl Choline, Leucocytes, and Erythrocyte were observed in 50/50+ and 0/100 + than the other diets. The Lysozyme activity was higher in 0/100+ and 50/50+ than the other diets.
In the Second feeding trial, fish meal was replaced by various carbohydrate sources on the growth performance and hepatic carbohydrate metabolic enzyme activities of the fingerlings of T. mossambicus. Five experimental diets were formulated to contain glucose, sucrose, maltose, dextrin, corn starch and control were maintained separately. The results indicated that the better weight gain, SGR, FCR and survival were also better in starch, dextrin and sucrose diet fed fish. There were significant differences in the total plasma, glucose and triglyceride concentration in fish fed with different carbohydrate sources. Plasma total protein, red blood cell, leucocytes and hemoglobin were significantly affected by various carbohydrate sources. The activities of glucose 6-Phosphate dehydrogenase, (G6PD), 6- Phospho fructokinase (PFK) and fructose 1, 6 – bisphosphatase (FBase) were significantly affected by these carbohydrate sources. While this two feeding trail indicated that the 50/50+ and 0/100+ of soy flour replacement with fish meal showed the optimum growth performance and in carbohydrate sources the corn starch, dextrose, and sucrose showed the better growth for tilapia fingerlings.
Effect of Replacement of Sweet Orange (Citrus Sinensis) Peel Meal with Maize ...IOSR Journals
This document summarizes a study that evaluated the effect of replacing maize offal with graded levels of sweet orange peel meal (SOPM) in diets for West African Dwarf goats. Sixteen goats were fed one of four diets containing 0%, 12.5%, 25%, or 50% SOPM for 84 days. Results showed no significant differences in nutrient digestibility or intake among the diets, except for ether extract intake which was lower for the 50% SOPM diet. The study concluded that SOPM can replace up to 50% of maize offal in goat diets without negatively impacting nutrient digestibility or intake.
This document describes a computer model developed to predict the responses of lactating ewes to concentrate supplementation. The model considers the effects of concentrate supplementation timing and levels on ruminal pH and forage intake. It simulates organic acid production from carbohydrate fermentation, saliva buffer production, and ruminal pH based on these factors. The model was able to predict the effects of increasing concentrate supplementation levels and timing on dry matter intake and forage substitution. Simulations suggested feeding concentrate in a single evening meal or in no more than two meals per day to minimize negative impacts on forage intake.
This study used Illumina sequencing of the 16S rRNA gene to analyze changes in the rumen microbiome of cattle subjected to feed restriction. The main findings were:
1) Feed restriction caused a large decrease in an abundant Succinivibrionaceae species and a significant increase in Methanobrevibacter gottschalkii.
2) Fifty-five days after being returned to a normal diet, the rumen microbiomes of previously restricted cattle were not different from controls, showing the disruption from restriction did not have long-term effects.
3) The rumen microbiome was not associated with compensatory growth in cattle 55 days after restriction ended.
metabolic acceleration and the evolution of human brainJoão Soares
Humans have larger brains, higher reproductive output, and longer lifespans than other great apes, presenting an "energetic paradox" that requires more energy expenditure. This study found that:
1) Humans have higher total daily energy expenditure (TEE) than chimpanzees, bonobos, gorillas and orangutans, even after controlling for factors like body size and physical activity.
2) Much of the increased human TEE is due to a higher basal metabolic rate (BMR), indicating increased metabolic activity of organs.
3) Humans also have a greater percentage of body fat than other great apes.
The findings suggest humans evolved an "accelerated
This document discusses amino acid interactions, specifically amino acid imbalances and antagonisms. It begins by defining imbalances and antagonisms based on Harper's 1956 definitions. Imbalances occur when supplementing all essential amino acids except the first limiting one, while antagonisms involve a structurally related amino acid increasing the requirement for another. The document then discusses several examples of imbalances and antagonisms, how they impact growth and amino acid requirements. It reviews research showing imbalances do not generally increase catabolism of the limiting amino acid, but antagonisms can. The document also discusses how imbalances and antagonisms depress food intake in animals given a choice.
Loss of the RNA polymerase III repressor Maf1 confers obesity resistanceAsh Byrnes
1) Mice with whole-body knockout of the RNA polymerase III repressor MAF1 are resistant to diet-induced obesity and nonalcoholic fatty liver disease. They have reduced food intake and increased metabolic inefficiency.
2) Energy expenditure is increased in MAF1 knockout mice through multiple mechanisms, including increased precursor tRNA synthesis and futile cycling of tRNAs and hepatic lipids.
3) Metabolite profiling reveals elevated levels of amino acids and spermidine in MAF1 knockout mice, linking induction of autophagy to their extended lifespan. Increased spermidine and reduced nicotinamide N-methyltransferase are associated with obesity resistance.
This study assessed the effects of minerals calcium, magnesium, and inorganic phosphorus on cyclicity in Nili Ravi buffaloes. 90 buffaloes were divided into cyclic (n=30), non-cyclic (n=30), and repeat breeders (n=30) groups. Blood samples were tested and calcium was significantly higher in cyclic buffaloes than non-cyclic and repeat breeders. Magnesium levels were similar in cyclic and non-cyclic buffaloes but significantly higher in repeat breeders. Inorganic phosphorus was imbalanced in non-cyclic and repeat breeders. The study concluded calcium and phosphorus should be in a 2:1 ratio and imbalances may result in infertility
Achieving factual sustainability in fish farming needs the addition of most of the fish meal exploited as feed stuffs. The current experiment described two feeding trials, that resulted in the complete replacement of fish meal in the fingerling of Tilapia mossambicus. The initial trial was accompanied with three stages of fish meal replacement (50, 75 and 100% of dietary protein) viz., one level of soy protein and two levels of Lactat ® Probiotic (0 (or) 0.3% of the diet). Since probiotic has been reported to promote gut health, it was incorporated inorder to examine the growth enhancement and whether it would ease high levels of fish meal replacement in T. mossambicus. Lipids were provided by Cod liver oil. The better weight gain was observed in the treatments 50/50+ of fish meal replacement and 0/100+ of fish meal replacement. The optimum Specific Growth Rate, Food Conversion Ratio and Survival were also observed in 50/50+ fish meal replacement and 0/100+ fish meal replacement. The higher serum Acetyl Choline, Leucocytes, and Erythrocyte were observed in 50/50+ and 0/100 + than the other diets. The Lysozyme activity was higher in 0/100+ and 50/50+ than the other diets.
In the Second feeding trial, fish meal was replaced by various carbohydrate sources on the growth performance and hepatic carbohydrate metabolic enzyme activities of the fingerlings of T. mossambicus. Five experimental diets were formulated to contain glucose, sucrose, maltose, dextrin, corn starch and control were maintained separately. The results indicated that the better weight gain, SGR, FCR and survival were also better in starch, dextrin and sucrose diet fed fish. There were significant differences in the total plasma, glucose and triglyceride concentration in fish fed with different carbohydrate sources. Plasma total protein, red blood cell, leucocytes and hemoglobin were significantly affected by various carbohydrate sources. The activities of glucose 6-Phosphate dehydrogenase, (G6PD), 6- Phospho fructokinase (PFK) and fructose 1, 6 – bisphosphatase (FBase) were significantly affected by these carbohydrate sources. While this two feeding trail indicated that the 50/50+ and 0/100+ of soy flour replacement with fish meal showed the optimum growth performance and in carbohydrate sources the corn starch, dextrose, and sucrose showed the better growth for tilapia fingerlings.
This document describes the development of a food web matrix for the Gulf of Mexico based on combining diet data from various sources. Predators and prey were grouped into 48 functional groups. Diet data from multiple studies were statistically analyzed using Dirichlet distributions to quantify the likely contributions of prey to predator diets and the associated error ranges. A hierarchical cluster analysis was performed to identify functional groups with similar diets. The revised food web was then compared to other published Gulf of Mexico food webs. Finally, an ecosystem model simulation using the revised diet matrix showed improved model fits compared to using the previous diet matrix.
An experimental study was carried over a
period of 42 days using 160 day-old commercial broiler chicks
(Ven Cobb) to study the effect of copper and flavomycin as feed
additives on growth performance in broiler chicken. The birds
were divided into four groups with four replicates of ten chicks in
each replicate. The treatment (T1) was control with standard
broiler ration and Cu as CuSO4 at 100 ppm, 200 ppm and
flavomycin at 10 ppm were supplemented in the basal diet to
form treatment 2 (T2), treatment 3 (T3) and treatment 4 (T4)
respectively.
The chemical composition of T1, T2, T3 and T4 rations
contained 15.43, 106.49, 212.54 and 18.69 ppm of Cu in broiler
starter and 12.67, 101.13, 202.72 and 20.20 ppm of Cu in broiler
finisher, respectively. The results of the present study revealed
that the supplementation of Cu as CuSO4 (100 and 200 ppm) or
flavomycin (10 ppm) showed no significant difference among the
treatment groups with regard to the feed consumption, body
weight gain and feed conversion ratio up to six weeks of age. The
carcass weight, slaughter weight, dressing percentage and giblet
yield were not influenced by dietary supplementation of either
Cu or flavomycin. It was concluded that dietary supplementation
of broiler chicken with Cu up to 200 ppm and flavomycin at 10
ppm did not have any adverse effect on production performance
of broiler chicken.
A study was conducted to determine if supplementing activated charcoal would increase intake of honey mesquite leaves by sheep. Twenty lambs were fed one of four levels of activated charcoal (0, 0.33, 0.67, 1 g/kg BW) along with low-quality hay and molasses. Lambs were given ad libitum access to honey mesquite leaves. Intake of mesquite leaves increased over time but did not differ between charcoal supplementation levels. Mesquite intake varied greatly among individual lambs. Hay intake and weight gain also did not differ between treatments. Supplementing activated charcoal did not increase mesquite leaf consumption in this study.
This study evaluated the effects of different dietary carbohydrate concentrations on nutrient digestibility and fecal scores in four African wildcats. The cats were fed one of four experimental diets varying in carbohydrate level from 10% to 44% over four 14-day periods. Results showed that carbohydrate concentration did not affect nutrient digestibility. However, the highest carbohydrate diet (44%) resulted in poorer fecal scores similar to diarrhea compared to the other diets. The study concludes that some carbohydrates are tolerated well by exotic felids, but the highest level altered fecal characteristics, suggesting a maximum carbohydrate level between 34-14% is best for African wildcats.
This document analyzes the relationship between body size and extinction risk in mammal species. It finds that both intrinsic and environmental factors that increase extinction risk scale sharply above a body mass threshold of around 3 kilograms. For smaller species, extinction risk is driven mainly by environmental factors related to geographic range, but for larger species both environmental factors and intrinsic biological traits are significant predictors of extinction risk. This suggests that the disadvantages of large body size for species survival are greater than previously thought.
This study assessed nutrient cycling processes in a constructed fen in northern Alberta, Canada. Four revegetation strategies were used: seedlings only, moss layer transfers, a combination of seedlings and moss, and an unplanted control. Above- and below-ground nutrient cycling processes were measured over two growing seasons and compared to a natural fen reference site. Microbially-mediated carbon, nitrogen, and phosphorus cycling processes were used as proxies to evaluate biogeochemical functioning. The results showed that revegetation increased both above-ground productivity and below-ground nutrient cycling, especially in plots with a diverse plant community established through a combination of seedlings and moss transfers. Revegetation also increased microbial activity and
The study examined the effects of dietary selenium level (0.3 ppm vs 3.0 ppm) and source (organic vs inorganic) on selenium retention in red blood cells and plasma in pigs over three time periods. The results showed that higher selenium levels led to greater selenium concentrations in red blood cells than plasma. Organic selenium was retained more in red blood cells than inorganic selenium. Selenium concentrations in both blood components increased over time from the nursery to finisher stages. The conclusions were supported by existing research showing the source and level of selenium supplementation affects blood selenium concentrations.
— Aim of this study was to obtain knowledge on boron supplemented diet to mineral status of body fluids and feces in short term nutrition of dairy cattle. A total of 24 healthy Holstein dairy cows were used. The animals were fed with standard ration, and boron at three different doses was added to experimental' diets as boron compound: borax, for 10 days. Boron and other macro and trace minerals were determined in serum, milk, urine and feces samples taken on 0 and 11 st days. In this study, there were no overt signs of toxicosis, and a pivotal knowledge was obtained in dairy cattle fed with boron supplemented diet on boron absorption, excretion, and its interaction with other minerals. Boron could not completely absorb from gastrointestinal tract. Urine was the most important excretion way of boron. More less boron was also eliminated by milk. Boron levels in body fluids (serum and milk, p<0.000) were increasingly changed based on the dose. Boron, among minerals, provided a striking increase for Ca (p<0.003) and Mg (p<0.028) levels in serum by increasing absorption of these minerals. This topic is worth evaluating as an alternative approach in the prevention of hypocalcemia in transition cows.
This document summarizes evidence from multiple disciplines that supports the hypothesis that early humans like Homo erectus had a highly carnivorous diet. The evidence discussed includes archaeological evidence showing early control of fire and competition between humans and large carnivores; isotopic, strontium, and barium analyses indicating early humans were top carnivores; anatomical comparisons showing similarities to carnivores; genetic adaptations related to meat consumption; parallels between human and wolf social structures and hunting behaviors; shorter human weaning times resembling carnivores more than chimpanzees; and ethnographic data on traditional diets of recent human groups being majority animal foods. The interdisciplinary evidence presented argues for a single Paleolithic diet for early humans
This study examined the effects of different dietary levels of betaine on growth performance, food efficiency, and survival rate of pike perch fingerlings over 6 weeks. Fingerlings were fed one of four diets: live food, biomar feed without betaine, biomar with 1% betaine, or biomar with 2% betaine. The results showed that fish fed 2% betaine biomar had significantly higher weight gain, specific growth rate, food efficiency, and lower food conversion ratio compared to other treatments. However, this treatment also had the highest cannibalism. The treatment with biomar only without betaine resulted in the highest survival rate of 34.5%. In conclusion, betaine improved growth but
Multidisciplinary attempt to reconstruct human nutrition through the Paleolithic shows that a highly carnivorous (meat based) diet was not only prevalent but essential to the existence of our species for almost 2 million years.
Effect of Varying the Energy Density of Protein-adequate Diets on Nutrient Me...Faisal A. Alshamiry
assessing the influence of varied dietary energy densities of a protein-adequate diet on immune response of growing Muzaffarnagari lambs besides nutritional performance and clinical chemistry indices.
Feeding rate requirements for Schilbe intermedius (Rüppel, 1832) fingerlings ...Innspub Net
The control of the breeding of Schilbe intermedius in captivity, passes by the determination of its nutritional requirements. The present study aims therefore to determine the optimal feed ration of the fingerlings of S. intermedius. The experiment was carried out in circular basins during 28 days. After their capture in natural environment, their transfer in controlled area and their acclimatization to the artificial food used (coppens: protein 45%.), the fingerlings used (average weight: 3.12±0.83g) were subjected to four feed rations (2, 5, 8 and
11% of the biomass) tested in triplicate each one. Thus, the lowest rate of survival (64.00±1.15%) was recorded for
the fingerlings’lot subjected to the ration of 8% where the pH is more acid (5.29).The final average weight (4.63±0.00g), the specific growth rate (1.19±0.08%/day) and the food effectiveness (0.53±0.00) obtained with the ration of 11% were the highest. These values are significantly different (P< 0.05) than those obtained with the ration of 2%.The best consumption index was registered with the ration of 2%. According to the model of Brett, the maximum and optimal daily rations of S. intermedius fingerlings were estimated to 4.6 % and 8.5 % respectively. Get the full articles at: http://www.innspub.net/volume-7-number-6-december-2015-ijaar/
Five diets (diets incorporated with folic acid, vitamin B, vitamin C, vitamin/
mineral mix and control) were used to determine their effects on survival, digestive
enzyme activity, protein profiling and growth of striped snakehead Channa striatus
fingerlings. All vitamin diets showed significant difference in enzyme activity like
protease, amylase and lipase, when compared to that of control. The fingerlings fed
with vitamin C diet showed significantly (P<0.05) better performance in terms of
survival rate, weight gain and Specific Growth Rate (SGR) when compared to the rest
of the four diets. The electrophoretic studies revealed that high molecular weight
fractions were added up in muscle tissue of C. striatus fed with vitamin and mineral
supplemented diets.
Use of Silage Acid Devil Fish (Pterygoplichthys spp.) as Protein Supplement i...criollito
Animal nutrition is one of the most important limiting factors in animal production, especially in ruminants, providing
proteins being the main constraint, due to the limited availability and high cost of protein sources (oilseed meals). Currently in the dam
“El Infiernillo” in Michoacán México, has a large population of devil fish (Pterygoplichthys spp.), which is an economic and ecological
problem, because it is not consumed by humans and causes pollution to be discarded directly into the environment. For that reason the
objective of this study was to evaluate the use of silage acid devil fish (SADF) in fattening beef cattle as a protein supplement. SADF is
defined as a product semi-liquid or pasty mixed with formic acid, which leads to a decrease in pH to near 4.0. Used 18 young bulls (Bos
taurus × Bos indicus) for 60 days with a starting weight of 278.9 ± 51.2 kg, housed in individual pens with food and water ad libitum
were randomly assigned to three treatments with different levels of inclusion SADF (0%, 12% and 18%). They were weighed to the
beginning of the experiment and later every 30 days, previous fasting of 24 hours. To determine the food consumption, weigh every day
the offered food and the surplus. There were no significant differences (P < 0.05) among treatments with different levels of inclusion of
SADF with respect to daily weight gain, with values of 952 ± 324, 927 ± 322 and 854 ± 307 g/day, respectively. The dry matter intake
(DMI) was 8.9, 9.3 and 7.7 kg/day to 0%, 12% and 18% of SADF, respectively. In the same values for feed conversion were 9.34, 10.03
and 9.01 kg DMI/kg of weigh live, and carcass yield of 60.6%, 60.3% and 58.5%, respectively. It is concluded that fish silage acid devil
is an excellent alternative in feeding beef cattle as a protein supplement.
This study tested different doses of phytase and calcium levels in corn/soybean meal-based diets for broilers with reduced nutrient density. Nine treatment groups were tested including a positive control diet based on breeder recommendations. All phytase treatment groups compensated for nutrient reductions and achieved equal or better growth performance than the positive control. Higher phytase doses of 2000 FTU/kg improved body weight gain and feed conversion ratio compared to 500 FTU/kg, while 1000 FTU/kg showed lower feed conversion. Reducing calcium levels in a stepwise manner lowered feed costs and improved energy efficiency, with the highest calcium reduction performing best. Bone ash was not affected. The results suggest that higher phyt
The document reanalyzes a previous study on patch selection by red deer in relation to energy and protein intake. The reanalysis uses an energy intake model that incorporates the effect of gut filling on daily intake rates. The results show that:
1) Patch use by red deer was just as strongly correlated with estimated daily intake of digestible energy as digestible protein in four of the seven trials studied.
2) In all cases, daily intake of digestible energy was a better predictor of patch preference than dry matter intake alone.
3) Estimated daily energy intake was highly correlated with short-term estimates of protein intake, suggesting patch use could maximize both energy and protein gain simultaneously.
- Nutrient enrichment decreased the carbon to phosphorus (C:P) ratio of detrital food resources like fine particulate organic matter (FPOM) and leaves in the treatment stream compared to the reference stream.
- A lower C:P ratio exacerbated carbon limitation for collectors but decreased phosphorus limitation for shredders.
- The nitrogen to phosphorus (N:P) ratio also decreased more in the treatment stream, indicating that microbes associated with detritus were taking up relatively more phosphorus and were more phosphorus limited.
Effects of Adding Different Levels of Phytase to Diet Containing Low Phosphor...Agriculture Journal IJOEAR
Abstract— This experiment was carried out to determine the effect of addition of different levels of phytase containing low phosphorus diets on performance, eggshell quality and bone characteristics in laying Japanese quail (Coturnix japonica). In the experiment, a total of 162 7-week-oldlaying Japanese quail were fed two different diets containing 0.33 % available phosphorus (AP, control) and 0.15 % AP supplemented with phytase 0, 500, 1000, 1500, 2000 phytase unites/g feed. Each diet was feed to groups having three replicates, each cages nine quail, in completely randomized design. Feed and water supplied for ad-libitum. Light was provided 17 hours daily. Egg production, egg mass, average daily feed consumption, viability, fat-free dried tibia weight were improved, as phytase level increased in the diet. However, final live weight, carcass weight, body weight gain, feed efficiency, tibia ash (%) and deformed egg ratio (%), some eggshell quality parameters measured in eggs collected at the end of the first period, were not affected by treatments. With regard to measured parameters, the responses of laying quail, to the increasing phytase levels, were not linear. The performance and bone parameters of the group fed with 1500 units were very close to that of the control group, even higher than the control group in some traits. Decreasing in shell weight of eggs collected at last three days of the experiment were not prevented any of the phytase levels. At the same period, shell thickness and breaking strength of the eggs of groups fed with 1500 and 2000 units phytase, were significantly lower than that of the control group. Manure ash percentage of phytase added groups were significantly lower than the control group.
This document discusses estimating the energy requirements for maintenance and growth of wild mammals, birds, and reptiles kept in captivity. It reviews existing data on basal metabolic rates and maintenance energy requirements for some species. The document develops a model to estimate lower limits of energy requirements during growth, taking into account factors like variation in time to reach maturity and energy density of tissue deposited. Having estimates of energy needs can help with issues like treating obesity or providing nutritional support when animals lose appetite. Overall, the document aims to provide guidance for estimating energy requirements when specific data is lacking for non-domesticated species in captive settings.
El documento describe el ENIAC, el primer ordenador electrónico completamente funcional. Fue creado en la Universidad de Pensilvania para realizar cálculos militares. Aunque era lento de programar, su ejecución era rápida. Estaba compuesto de 18.000 válvulas electrónicas que le permitían operar más rápido que calculadoras mecánicas previas, aunque también causaban frecuentes averías y un alto consumo eléctrico. El ENIAC marcó el comienzo de los ordenadores modernos y su evol
This document describes the development of a food web matrix for the Gulf of Mexico based on combining diet data from various sources. Predators and prey were grouped into 48 functional groups. Diet data from multiple studies were statistically analyzed using Dirichlet distributions to quantify the likely contributions of prey to predator diets and the associated error ranges. A hierarchical cluster analysis was performed to identify functional groups with similar diets. The revised food web was then compared to other published Gulf of Mexico food webs. Finally, an ecosystem model simulation using the revised diet matrix showed improved model fits compared to using the previous diet matrix.
An experimental study was carried over a
period of 42 days using 160 day-old commercial broiler chicks
(Ven Cobb) to study the effect of copper and flavomycin as feed
additives on growth performance in broiler chicken. The birds
were divided into four groups with four replicates of ten chicks in
each replicate. The treatment (T1) was control with standard
broiler ration and Cu as CuSO4 at 100 ppm, 200 ppm and
flavomycin at 10 ppm were supplemented in the basal diet to
form treatment 2 (T2), treatment 3 (T3) and treatment 4 (T4)
respectively.
The chemical composition of T1, T2, T3 and T4 rations
contained 15.43, 106.49, 212.54 and 18.69 ppm of Cu in broiler
starter and 12.67, 101.13, 202.72 and 20.20 ppm of Cu in broiler
finisher, respectively. The results of the present study revealed
that the supplementation of Cu as CuSO4 (100 and 200 ppm) or
flavomycin (10 ppm) showed no significant difference among the
treatment groups with regard to the feed consumption, body
weight gain and feed conversion ratio up to six weeks of age. The
carcass weight, slaughter weight, dressing percentage and giblet
yield were not influenced by dietary supplementation of either
Cu or flavomycin. It was concluded that dietary supplementation
of broiler chicken with Cu up to 200 ppm and flavomycin at 10
ppm did not have any adverse effect on production performance
of broiler chicken.
A study was conducted to determine if supplementing activated charcoal would increase intake of honey mesquite leaves by sheep. Twenty lambs were fed one of four levels of activated charcoal (0, 0.33, 0.67, 1 g/kg BW) along with low-quality hay and molasses. Lambs were given ad libitum access to honey mesquite leaves. Intake of mesquite leaves increased over time but did not differ between charcoal supplementation levels. Mesquite intake varied greatly among individual lambs. Hay intake and weight gain also did not differ between treatments. Supplementing activated charcoal did not increase mesquite leaf consumption in this study.
This study evaluated the effects of different dietary carbohydrate concentrations on nutrient digestibility and fecal scores in four African wildcats. The cats were fed one of four experimental diets varying in carbohydrate level from 10% to 44% over four 14-day periods. Results showed that carbohydrate concentration did not affect nutrient digestibility. However, the highest carbohydrate diet (44%) resulted in poorer fecal scores similar to diarrhea compared to the other diets. The study concludes that some carbohydrates are tolerated well by exotic felids, but the highest level altered fecal characteristics, suggesting a maximum carbohydrate level between 34-14% is best for African wildcats.
This document analyzes the relationship between body size and extinction risk in mammal species. It finds that both intrinsic and environmental factors that increase extinction risk scale sharply above a body mass threshold of around 3 kilograms. For smaller species, extinction risk is driven mainly by environmental factors related to geographic range, but for larger species both environmental factors and intrinsic biological traits are significant predictors of extinction risk. This suggests that the disadvantages of large body size for species survival are greater than previously thought.
This study assessed nutrient cycling processes in a constructed fen in northern Alberta, Canada. Four revegetation strategies were used: seedlings only, moss layer transfers, a combination of seedlings and moss, and an unplanted control. Above- and below-ground nutrient cycling processes were measured over two growing seasons and compared to a natural fen reference site. Microbially-mediated carbon, nitrogen, and phosphorus cycling processes were used as proxies to evaluate biogeochemical functioning. The results showed that revegetation increased both above-ground productivity and below-ground nutrient cycling, especially in plots with a diverse plant community established through a combination of seedlings and moss transfers. Revegetation also increased microbial activity and
The study examined the effects of dietary selenium level (0.3 ppm vs 3.0 ppm) and source (organic vs inorganic) on selenium retention in red blood cells and plasma in pigs over three time periods. The results showed that higher selenium levels led to greater selenium concentrations in red blood cells than plasma. Organic selenium was retained more in red blood cells than inorganic selenium. Selenium concentrations in both blood components increased over time from the nursery to finisher stages. The conclusions were supported by existing research showing the source and level of selenium supplementation affects blood selenium concentrations.
— Aim of this study was to obtain knowledge on boron supplemented diet to mineral status of body fluids and feces in short term nutrition of dairy cattle. A total of 24 healthy Holstein dairy cows were used. The animals were fed with standard ration, and boron at three different doses was added to experimental' diets as boron compound: borax, for 10 days. Boron and other macro and trace minerals were determined in serum, milk, urine and feces samples taken on 0 and 11 st days. In this study, there were no overt signs of toxicosis, and a pivotal knowledge was obtained in dairy cattle fed with boron supplemented diet on boron absorption, excretion, and its interaction with other minerals. Boron could not completely absorb from gastrointestinal tract. Urine was the most important excretion way of boron. More less boron was also eliminated by milk. Boron levels in body fluids (serum and milk, p<0.000) were increasingly changed based on the dose. Boron, among minerals, provided a striking increase for Ca (p<0.003) and Mg (p<0.028) levels in serum by increasing absorption of these minerals. This topic is worth evaluating as an alternative approach in the prevention of hypocalcemia in transition cows.
This document summarizes evidence from multiple disciplines that supports the hypothesis that early humans like Homo erectus had a highly carnivorous diet. The evidence discussed includes archaeological evidence showing early control of fire and competition between humans and large carnivores; isotopic, strontium, and barium analyses indicating early humans were top carnivores; anatomical comparisons showing similarities to carnivores; genetic adaptations related to meat consumption; parallels between human and wolf social structures and hunting behaviors; shorter human weaning times resembling carnivores more than chimpanzees; and ethnographic data on traditional diets of recent human groups being majority animal foods. The interdisciplinary evidence presented argues for a single Paleolithic diet for early humans
This study examined the effects of different dietary levels of betaine on growth performance, food efficiency, and survival rate of pike perch fingerlings over 6 weeks. Fingerlings were fed one of four diets: live food, biomar feed without betaine, biomar with 1% betaine, or biomar with 2% betaine. The results showed that fish fed 2% betaine biomar had significantly higher weight gain, specific growth rate, food efficiency, and lower food conversion ratio compared to other treatments. However, this treatment also had the highest cannibalism. The treatment with biomar only without betaine resulted in the highest survival rate of 34.5%. In conclusion, betaine improved growth but
Multidisciplinary attempt to reconstruct human nutrition through the Paleolithic shows that a highly carnivorous (meat based) diet was not only prevalent but essential to the existence of our species for almost 2 million years.
Effect of Varying the Energy Density of Protein-adequate Diets on Nutrient Me...Faisal A. Alshamiry
assessing the influence of varied dietary energy densities of a protein-adequate diet on immune response of growing Muzaffarnagari lambs besides nutritional performance and clinical chemistry indices.
Feeding rate requirements for Schilbe intermedius (Rüppel, 1832) fingerlings ...Innspub Net
The control of the breeding of Schilbe intermedius in captivity, passes by the determination of its nutritional requirements. The present study aims therefore to determine the optimal feed ration of the fingerlings of S. intermedius. The experiment was carried out in circular basins during 28 days. After their capture in natural environment, their transfer in controlled area and their acclimatization to the artificial food used (coppens: protein 45%.), the fingerlings used (average weight: 3.12±0.83g) were subjected to four feed rations (2, 5, 8 and
11% of the biomass) tested in triplicate each one. Thus, the lowest rate of survival (64.00±1.15%) was recorded for
the fingerlings’lot subjected to the ration of 8% where the pH is more acid (5.29).The final average weight (4.63±0.00g), the specific growth rate (1.19±0.08%/day) and the food effectiveness (0.53±0.00) obtained with the ration of 11% were the highest. These values are significantly different (P< 0.05) than those obtained with the ration of 2%.The best consumption index was registered with the ration of 2%. According to the model of Brett, the maximum and optimal daily rations of S. intermedius fingerlings were estimated to 4.6 % and 8.5 % respectively. Get the full articles at: http://www.innspub.net/volume-7-number-6-december-2015-ijaar/
Five diets (diets incorporated with folic acid, vitamin B, vitamin C, vitamin/
mineral mix and control) were used to determine their effects on survival, digestive
enzyme activity, protein profiling and growth of striped snakehead Channa striatus
fingerlings. All vitamin diets showed significant difference in enzyme activity like
protease, amylase and lipase, when compared to that of control. The fingerlings fed
with vitamin C diet showed significantly (P<0.05) better performance in terms of
survival rate, weight gain and Specific Growth Rate (SGR) when compared to the rest
of the four diets. The electrophoretic studies revealed that high molecular weight
fractions were added up in muscle tissue of C. striatus fed with vitamin and mineral
supplemented diets.
Use of Silage Acid Devil Fish (Pterygoplichthys spp.) as Protein Supplement i...criollito
Animal nutrition is one of the most important limiting factors in animal production, especially in ruminants, providing
proteins being the main constraint, due to the limited availability and high cost of protein sources (oilseed meals). Currently in the dam
“El Infiernillo” in Michoacán México, has a large population of devil fish (Pterygoplichthys spp.), which is an economic and ecological
problem, because it is not consumed by humans and causes pollution to be discarded directly into the environment. For that reason the
objective of this study was to evaluate the use of silage acid devil fish (SADF) in fattening beef cattle as a protein supplement. SADF is
defined as a product semi-liquid or pasty mixed with formic acid, which leads to a decrease in pH to near 4.0. Used 18 young bulls (Bos
taurus × Bos indicus) for 60 days with a starting weight of 278.9 ± 51.2 kg, housed in individual pens with food and water ad libitum
were randomly assigned to three treatments with different levels of inclusion SADF (0%, 12% and 18%). They were weighed to the
beginning of the experiment and later every 30 days, previous fasting of 24 hours. To determine the food consumption, weigh every day
the offered food and the surplus. There were no significant differences (P < 0.05) among treatments with different levels of inclusion of
SADF with respect to daily weight gain, with values of 952 ± 324, 927 ± 322 and 854 ± 307 g/day, respectively. The dry matter intake
(DMI) was 8.9, 9.3 and 7.7 kg/day to 0%, 12% and 18% of SADF, respectively. In the same values for feed conversion were 9.34, 10.03
and 9.01 kg DMI/kg of weigh live, and carcass yield of 60.6%, 60.3% and 58.5%, respectively. It is concluded that fish silage acid devil
is an excellent alternative in feeding beef cattle as a protein supplement.
This study tested different doses of phytase and calcium levels in corn/soybean meal-based diets for broilers with reduced nutrient density. Nine treatment groups were tested including a positive control diet based on breeder recommendations. All phytase treatment groups compensated for nutrient reductions and achieved equal or better growth performance than the positive control. Higher phytase doses of 2000 FTU/kg improved body weight gain and feed conversion ratio compared to 500 FTU/kg, while 1000 FTU/kg showed lower feed conversion. Reducing calcium levels in a stepwise manner lowered feed costs and improved energy efficiency, with the highest calcium reduction performing best. Bone ash was not affected. The results suggest that higher phyt
The document reanalyzes a previous study on patch selection by red deer in relation to energy and protein intake. The reanalysis uses an energy intake model that incorporates the effect of gut filling on daily intake rates. The results show that:
1) Patch use by red deer was just as strongly correlated with estimated daily intake of digestible energy as digestible protein in four of the seven trials studied.
2) In all cases, daily intake of digestible energy was a better predictor of patch preference than dry matter intake alone.
3) Estimated daily energy intake was highly correlated with short-term estimates of protein intake, suggesting patch use could maximize both energy and protein gain simultaneously.
- Nutrient enrichment decreased the carbon to phosphorus (C:P) ratio of detrital food resources like fine particulate organic matter (FPOM) and leaves in the treatment stream compared to the reference stream.
- A lower C:P ratio exacerbated carbon limitation for collectors but decreased phosphorus limitation for shredders.
- The nitrogen to phosphorus (N:P) ratio also decreased more in the treatment stream, indicating that microbes associated with detritus were taking up relatively more phosphorus and were more phosphorus limited.
Effects of Adding Different Levels of Phytase to Diet Containing Low Phosphor...Agriculture Journal IJOEAR
Abstract— This experiment was carried out to determine the effect of addition of different levels of phytase containing low phosphorus diets on performance, eggshell quality and bone characteristics in laying Japanese quail (Coturnix japonica). In the experiment, a total of 162 7-week-oldlaying Japanese quail were fed two different diets containing 0.33 % available phosphorus (AP, control) and 0.15 % AP supplemented with phytase 0, 500, 1000, 1500, 2000 phytase unites/g feed. Each diet was feed to groups having three replicates, each cages nine quail, in completely randomized design. Feed and water supplied for ad-libitum. Light was provided 17 hours daily. Egg production, egg mass, average daily feed consumption, viability, fat-free dried tibia weight were improved, as phytase level increased in the diet. However, final live weight, carcass weight, body weight gain, feed efficiency, tibia ash (%) and deformed egg ratio (%), some eggshell quality parameters measured in eggs collected at the end of the first period, were not affected by treatments. With regard to measured parameters, the responses of laying quail, to the increasing phytase levels, were not linear. The performance and bone parameters of the group fed with 1500 units were very close to that of the control group, even higher than the control group in some traits. Decreasing in shell weight of eggs collected at last three days of the experiment were not prevented any of the phytase levels. At the same period, shell thickness and breaking strength of the eggs of groups fed with 1500 and 2000 units phytase, were significantly lower than that of the control group. Manure ash percentage of phytase added groups were significantly lower than the control group.
This document discusses estimating the energy requirements for maintenance and growth of wild mammals, birds, and reptiles kept in captivity. It reviews existing data on basal metabolic rates and maintenance energy requirements for some species. The document develops a model to estimate lower limits of energy requirements during growth, taking into account factors like variation in time to reach maturity and energy density of tissue deposited. Having estimates of energy needs can help with issues like treating obesity or providing nutritional support when animals lose appetite. Overall, the document aims to provide guidance for estimating energy requirements when specific data is lacking for non-domesticated species in captive settings.
El documento describe el ENIAC, el primer ordenador electrónico completamente funcional. Fue creado en la Universidad de Pensilvania para realizar cálculos militares. Aunque era lento de programar, su ejecución era rápida. Estaba compuesto de 18.000 válvulas electrónicas que le permitían operar más rápido que calculadoras mecánicas previas, aunque también causaban frecuentes averías y un alto consumo eléctrico. El ENIAC marcó el comienzo de los ordenadores modernos y su evol
The document discusses Active Record attributes in Rails 5. It explains that Active Record's detected attribute types can be overridden with the attribute method. The attribute method can also define a default value and specify if an attribute is an array or range. Attributes do not require a matching database column. Examples demonstrate overriding an attribute type from decimal to integer, setting default values, and defining array attributes.
This training presentation discusses consumption approval and fabric inspection. It provides formulas and steps for calculating consumption for pants and shirts. It also outlines the 8 steps for fabric inspection, including calculating the amount to check, choosing rolls, identifying problems, and preparing a defect report. Fabric problems can arise from spinning, weaving or processing faults. Fabric will be rejected if the penalty percentage is over 40, the roll length is below 25 yards, joint parts are less than 25 yards, or the shade does not match the approved swatch.
The most common diabetes symptoms include frequent urination, intense thirst and hunger, weight gain, unusual weight loss, fatigue, cuts and bruises that do not heal, male sexual dysfunction, numbness and tingling in hands and feet.
Proteins contribute significantly to the physical properties of foods, allowing them to build or stabilize gels, foams, doughs, emulsions, and fibrillar structures. Proteins act as foam-forming and foam-stabilizing agents by forming flexible films around gas bubbles that decrease surface tension. The stability of foams, emulsions, and gels depends on factors like the protein's ability to diffuse, denature, and associate at interfaces. Proteins can also increase viscosity, bind flavors, form dough networks, and exhibit antioxidant properties depending on their amino acid composition and structure.
this presentation is in two sections, 1st one is about protein quality estimation and 2nd is about novel protein sources.
hope it would be helpful for u guys...
Phosphorus digestibility and relative phosphorus bioavailability in two dried...SaadAldin2
Phosphorus digestibility and relative phosphorus bioavailability in two dried black soldier fly larvae meals and a defatted black soldier fly larvae meal in broiler chickens
1
5
Does Protein Affect the Metabolic Rate of ZebraFish
Statement of the Problem
Dietary ingredients, and nutrients as well as anti-nutritional remain important factors that affect its growth and development. However, there lacks proper nutritional control due to the absence of standardized reference diet (Boyle et al. 5354). Moreover, numerous epidemiological studies undertaken indicate that several prenatal are important in the growth and development of Zebrafish (Daniorerio)(Acheson et al. 456) As a way of providing a standardized dietary framework, a need emerges for the provision of specific dietary and nutritional standard aimed at improving the growth and development of Zebra fish. Thus, the necessity to investigate the growth and metabolic rate of Zebrafish (Daniorerio) when fed with different commercial diets.
BACKGROUND
The consumption of proteins in a diet could have significant effects on the body metabolism. The high calories contents of proteins commonly require more calories for breakdown that carbohydrates and this could have significant effects of various metabolic aspects. The amount nature of proteins makes them significantly different from other dietary constitutes that people consume routinely. In general, proteins cause an increase in the metabolic rate of organisms because of a variety of reasons Like for instance, Weight maintenance is actually a simple matter of the energy balance and also energy balance is attained when "energy in" is corresponding to "energy out
Thermic affects accounts for the numerous ways in which proteins increase the metabolic rate of the body. Due to the strong bond making the protein molecules, a lot of energy is required to break down the high protein that has been consumed. The thermic effect of proteins means that about 30% of the calories in proteins are burned in the process of digesting proteins(Acheson et al. 528). The heat generated in the process increases the general body heat output hence influencing metabolism.
The consumption of protein in diet has been established as a factor that increases the metabolic rate in human beings (Howard et al. 117).This is mainly because proteins require a lot of energy to be broken down and the body must provide such energy, hence it increase the metabolic rate. The process of protein synthesis in the body causes the amino acid structures of the proteins consumed to begin the process of building body muscles. The construction of these tissues in the body is a process that consumes high amounts of energy hence the body requires increased energy to undertaken the process. As a result on the increased energy requirements, high metabolic rate occurs in order to maintain the required amount of energy necessary to sustain the activities of breaking down amino acids to form muscle tissues.
Within marine environments, hypoxia episodes remain prevalent due to a variety of reasons. The presence of oxygen within the marine environmen ...
Quantifying phytate in dairy digesta and feces- Alkaline extraction and high-...Partha Ray
This document describes the development of a method to accurately quantify undigested phytate in ruminant feces and digesta. Samples were collected from dairy heifers and cows fed diets varying in phytate content to challenge extraction and quantification methods. Alkaline extraction using NaOH and EDTA yielded greater estimates of phytate in digesta and fecal samples than acid extraction, but alkaline extracts cannot be directly analyzed by high-performance ion chromatography (HPIC) due to matrix interference. The proposed method combines the extraction power of alkaline extraction with the precision of HPIC analysis by acidifying alkaline extracts and filtering to remove matrix interference before HPIC quantification. Validation tests found near complete recovery of added phyt
Organic meat contains significantly higher levels of nutritionally desirable polyunsaturated and omega-3 fatty acids and lower levels of the potentially harmful saturated fatty acids myristic acid and palmitic acid compared to non-organic meat. This is likely due to differences in feeding practices required by organic standards which emphasize forage-based diets for livestock. Switching to organic meat could allow for a 30% reduction in meat consumption while maintaining omega-3 intake levels or a 50% increase in meat-based omega-3 intake at the same consumption level. Further research is still needed to fully understand compositional differences in individual meat types and a wider range of nutrients and compounds.
Evaluation of heat treated small ruminants dropping as an alternative feedstu...Alexander Decker
This study evaluated heat-treated small ruminant droppings (SRD) as an alternative feedstuff in weanling pig diets. 144 crossbred piglets were fed one of four diets: a control diet with 0% SRD or test diets with 10%, 30%, or 50% SRD. Results showed SRD contains protein, fat, fiber and ash. Feed intake increased with SRD level, while weight gain and protein efficiency decreased at 30-50% SRD. Carcass characteristics were unaffected except for thigh weight. Digestibility of nutrients improved in test diets compared to control. The study concludes SRD is a potential feedstuff for pigs up to 50% dietary level.
Changes in rumen bacterial fermentation are caused by a combined effect of fo...cheerfulnucleus73
This document summarizes a study that examined the effects of rumen pH and diet type on rumen bacterial fermentation in vitro. Eight continuous culture fermenters were used to study two diet types (60% forage/40% concentrate vs 10% forage/90% concentrate) at eight pH levels ranging from 4.9 to 7. The results showed that rumen bacterial fermentation, including nutrient digestion and volatile fatty acid production, was affected by both pH and diet type independently as well as through their interaction. The study provides insight into separating the effects of pH versus substrate on rumen fermentation.
Taking NIR beyond feedstuffs - analysis to enhance pork production profitabilityMilling and Grain magazine
Swine production has been facing substantial economic challenges in recent years, due to poor crop yields and increased competition for raw materials from the biofuel industry. As a consequence, feed prices have been variable and more industrial by-products have become available. At the same time, we have experienced increasing sustainability demands on animal production, for example to reduce nutrient release in effluent, while producing more and cheaper food for an increasing world population. All this has driven the swine industry to implement more professional, accurate and precise practises.
This document summarizes a study that estimated choline intake in European populations using food consumption data from 12 European countries and choline composition data from the USDA database. The study found that average choline intake ranged from 151-444 mg/day across different age groups, with intake generally below the adequate intake levels set by the IOM. The main food groups contributing to choline intake were meat, milk, grains, eggs and derived products, composite dishes, and fish. Limitations included the lack of choline composition data for European foods requiring the use of USDA data instead.
Current challenges and opportunities in amino acid nutrition of salmonidsInternational Aquafeed
Fishmeal is still one of the main protein sources used in commercial feeds for trout and salmon. But its availability is shrinking and its cost is increasing year by year. The sustainability of the aquaculture industry depends largely on its capability to replace fishmeal with alternative sources of protein, and to reduce the currently excessive protein levels commonly applied in the formulation of commercial diets. At the same time, feeds must be formulated to be effective in covering the nutrient requirements of specific species in order to maximise growth.
Disappearance of infused phytate from the large intestine of dairy heifersPartha Ray
This study investigated the degradation of phytate (a form of phosphorus) in the large intestine of dairy heifers. Eight heifers with ruminal and ileal cannulas were fed a low-phosphorus diet and infused with varying amounts of phytate into the ileum. Feces were collected and analyzed for phytate, inorganic phosphorus, and total phosphorus. Results showed that phytate was degraded but not completely in the large intestine. Fecal phosphorus excretion increased with higher amounts of infused phytate. Approximately 44% of phosphorus from dietary sources flowing to the ileum was absorbed in the large intestine, compared to 25% from infused pure phytate
The effects of forage particle length and exogenous phytase inclusion on phos...Partha Ray
This study investigated the effects of supplemental phytase and forage particle length on phosphorus digestion in lactating dairy cows. Five cannulated cows were fed diets with or without supplemental phytase and with either short or long forage particle length in a 2x2 factorial design. Samples of feed, digesta, feces and urine were collected and analyzed for phosphorus fractions. Total phosphorus intake was unaffected by diet, but phytate intake decreased and inorganic phosphorus intake increased with supplemental phytase. Fecal excretion of phytate decreased with phytase supplementation. However, phytase supplementation did not affect site or extent of phosphorus digestion, likely because the cows were late-lact
Application of digestibility values in poultry and bioassay and analytical procedures using poultry
Sri Venkateswara veterinary university
Animal nutrition
Vishnu Vardhan Reddy
Comparative Effect of Some Proprietary Vitamins and Trace Mineral Mixtures on...iosrjce
The research study was carried out to evaluate the comparative effect of some proprietary
vitamins/trace mineral mixtures on the performance and egg quality of laying hens. Two hundred and fifty- six
fifty weeks old Yaffa strain laying birds were used. The birds were randomly allotted to four premixes fortified
experimental diets; PPGODOMIX, PPOMIX, PPROCHE and PPWRITEX with each diet containing isocaloric
and isoproteinous. Sixty four birds were randomly assigned to each of the dietary treatments. The birds were
further sub- divided into four replicates with sixteen birds per replicate. The four dietary treatments elicited no
significant (P>0.05) influence on the overall performance of birds. Percentage hen day egg production was
equally not significantly (P>0.05) affected by any of the dietary treatments examined. The average daily feed
intake was significantly (P<0.05)><0.05)>0.05) influenced by PPOMIX. Birds fed PPOMIX obtained a significantly (P<0.05) higher
value of egg weight, albumen weight and blood spot which were statistically similar to other experimental
premixes examined. The results generated from this study revealed that premix of similar profile will give
similar result which could be used to improve ration management towards enhanced feed utilization and egg
internal/external quality.
This document summarizes a study that investigated the relationship between plasma iron concentration and gastric pH in captive bottlenose dolphins. The study found:
1) There was a strong negative correlation (r = -0.85) between plasma iron concentration and gastric pH in the dolphins, suggesting dolphins with lower gastric pH had higher plasma iron concentration.
2) Gastric pH and plasma iron concentration did not differ significantly over time within individual dolphins or among dolphin pools.
3) Hemosiderosis, an excessive iron storage condition, has been diagnosed in some captive dolphin populations, and gastric pH may play an important role in iron absorption in dolphins.
This study evaluated the lysine requirements of feedlot cattle. Sixty steer calves were individually fed increasing levels of rumen-protected lysine to determine the optimal supplementation level. Addition of lysine and methionine improved gains, intakes, and efficiency during the first 56 days, but methionine alone had no effect, suggesting lysine was limiting. Maximum performance was seen at 3-4 g/day of supplemental lysine, predicting a requirement of 58.4 g/day or 5.58% of metabolizable protein.
The document summarizes a study that compared three methods of acid-base analysis - the traditional approach, Stewart approach, and a semiquantitative approach. The study evaluated 98 dogs and cats presenting to an emergency room. The traditional approach identified respiratory abnormalities in 14 animals and metabolic abnormalities in 67 animals, with a mixed disorder being most common. The Stewart approach identified more metabolic abnormalities than the traditional approach. The semiquantitative approach identified abnormalities in all cases. The physicochemical approaches diagnosed more abnormalities than the traditional approach, though many were small and clinically irrelevant. The physicochemical approaches may provide greater insight into underlying etiologies, particularly for albumin and phosphorus abnormalities.
This document summarizes a study on the effects of Pallisentis nagpurensis infection on protein and free amino acid metabolism in two commercially important fish species, Catla catla and Labeo rohita. The study found that total protein content and free amino acid levels increased in the liver and intestine tissues of both infected fish species compared to normal uninfected fish. This suggests the parasitic infection altered the protein metabolism of the fish, likely to support tissue repair mechanisms in response to the parasite invasion. Overall, the study demonstrates that P. nagpurensis infection biochemically impacts the host fish by changing their protein and amino acid metabolism.
Similar to Florin_et_al-2011-Functional_Ecology (20)
1. The biological basis for understanding and predicting
dietary-induced variation in nitrogen and sulphur
isotope ratio discrimination
Scott T. Florin*,1
, Laura A. Felicetti2
and Charles T. Robbins1,2
1
School of Biological Sciences, Washington State University, Pullman, Washington 99164-4236, USA; and 2
Department
of Natural Resource Sciences, Washington State University, Pullman, Washington 99164-4236, USA
Summary
1. Accurately predicting isotope ratio discrimination is central to using mixing models to esti-
mate assimilated diets of wild animals. This process is complicated when omnivores consume
mixed diets because their discrimination is unlikely to be the weighted average of the various die-
tary constituents as occurs in current models.
2. We sought a basic understanding of how protein quality and quantity determine D15
N and
D34
S in mammals and birds. Dietary protein is the primary source of both elements in many
plants and animals. Low protein quality and high protein content have the potential to increase
D15
N by increasing protein turnover.
3. Protein quality, defined as the relative amount of the most limiting amino acid, accounted for
87–90% of the variation in D15
N when mammals and birds consumed plant matter and mixed
diets of plants and animals with protein of intermediate quality and quantity. However, foods
containing relatively large amounts of high quality protein (i.e. vertebrate flesh) and foods with
exceptionally low quality protein (e.g. lentils, Lens culinaris) had disparate nitrogen discrimina-
tions relative to what would be predicted from protein quality alone.
4. Supplementation of plant and animal diets with nitrogen-free carbohydrates and fats to
reduce protein quantity did not reduce D15
N in three plant-based diets fed to laboratory rats, but
reduced D15
N in two of three meat diets with >50% protein.
5. D34
S was weakly correlated with D15
N (R2
= 0Æ48) but was highly correlated with dietary
d34
S (R2
= 0Æ89). Because methionine, a sulphur amino acid, was the most limiting amino acid
in all diets, sulphur should be highly conserved as indicated by the lack of any change in D34
S
when diets were supplemented with carbohydrates and fat to both provide additional energy and
reduce protein content.
6. Predictive equations incorporating both protein quality and quantity accounted for 81% of
the variation in D15
N and offer the opportunity to create more realistic mixing models to accu-
rately estimate assimilated diets for omnivores.
Key-words: assimilated diet, isotope discrimination, nitrogen, protein quality, stable isotopes,
sulphur
Introduction
Accurately predicting isotopic discrimination is central to
estimating assimilated diets of wild animals when using sta-
ble isotopes (Martinez del Rio et al. 2009). While many
studies have postulated or identified causes of variation in
discrimination (Fantle et al. 1999; Roth & Hobson 2000;
McCutchan et al. 2003; Pearson et al. 2003; Vanderklift &
Ponsard 2003; Gaye-Siessegger et al. 2004, 2007; Robbins,
Felicetti & Sponheimer 2005; Miron et al. 2006; Caut, Ang-
ulo & Courchamp 2008, 2009; Tsahar et al. 2008; Robbins,
Felicetti & Florin 2010; Smith et al. 2010), none have pro-
posed cause-effect biologically based models for accurately
estimating unknown discriminations even though selection
of discrimination values is the single most important
assumption determining assimilated diet estimates. The lack*Correspondence author. E-mail: sflorin@wsu.edu
Ó 2010 The Authors. Functional Ecology Ó 2010 British Ecological Society
Functional Ecology 2011, 25, 519–526 doi: 10.1111/j.1365-2435.2010.01799.x
2. of such models to accurately predict nitrogen, carbon, or
sulphur discriminations, particularly for foods in mixed
diets, may lead to unacceptable errors in estimating assimi-
lated diets of ancestral humans and wild animals (Caut,
Angulo & Courchamp 2008; Robbins, Felicetti & Florin
2010).
Current approaches to estimating unknown discrimina-
tions for foods consumed by free-ranging animals include:
(i) feeding wild-collected foods to captive animals and directly
measuring their discrimination, which is not always feasible
and may rarely simulate field complexity; (ii) using a grand
mean for all foods (e.g. 2Æ0–3Æ4& for nitrogen and 0& for
sulphur), which ignores the three- to fourfold variation in
D15
N (e.g. c. )2 to 6&) and D34
S (e.g. c. )3 to 8&); or (iii)
using various regressions between dietary isotope values and
discriminations that have been determined with captive wild-
life consuming a wide range of foods, which describe very
general relationships that may not be cause-effect (Peterson
& Fry 1987; McCutchan et al. 2003; Vanderklift & Ponsard
2003; Robbins, Felicetti & Sponheimer 2005; Caut, Angulo &
Courchamp 2009; Martinez del Rio et al. 2009; Robbins,
Felicetti & Florin 2010).
Two major hypotheses have been proposed to explain
much of the dietary-induced variation in D15
N. The protein
quantity hypothesis suggests that as dietary protein content
(%) or intake (g day)1
) increase, D15
N will increase (Pearson
et al. 2003; Martinez del Rio et al. 2009). The protein quality
hypothesis suggests that as protein quality decreases, D15
N
will increase (Roth & Hobson 2000; Robbins, Felicetti &
Sponheimer 2005; Robbins, Felicetti & Florin 2010). Both
are based on the observation or hypothesis that as dietary
protein intake or amino acid scavenging increase, nitrogen
excretion will increase and lead to the preferential retention of
15
N which will elevate the animal’s d15
N value relative to the
diet.
Although Robbins, Felicetti & Sponheimer (2005) and
Robbins, Felicetti & Florin (2010) found no support for the
protein quantity hypothesis when plotting either nitrogen
content or carbon : nitrogen ratios against D15
N, such plots
are confounded by lower protein, largely plant-based diets of
poorer protein quality at one end of the regression and higher
protein, largely animal-based diets of higher protein quality
at the other. If both protein quality and quantity are impor-
tant, D15
N may be elevated when plant-based diets are con-
sumed primarily because of their poorer protein quality and
when animal-based diets are consumed primarily because of
their higher protein content. Thus, we hypothesized that both
protein quality and quantity may be important, but the rela-
tionships are more complex than either proposal alone sug-
gests.
Thus far, little use has been made of sulphur isotopes for
estimating assimilated diet, although the consumer’s isotope
value should reflect the dietary isotope value (Felicetti et al.
2003; McCutchan et al. 2003; Arneson & MacAvoy 2005).
D15
N and D34
S may be related in that sulphur amino acids
(methionine, cystine, cysteine and taurine) are important
sources of organic sulphur (Arneson & MacAvoy 2005).
If sulphur amino acids are important in determining protein
quality, dietary sulphur amino acid content may be important
in determining both D15
N and D34
S. Consequently, we sought
a unified concept incorporating both protein quality and
quantity that could be used to understand and accurately pre-
dict D15
N, D34
S and assimilated diets of omnivores.
Materials and methods
QUANTIF YING PROTEIN QUANT ITY AND QUALIT Y
While nitrogen or protein quantity (N · 6Æ25) has been measured in
virtually all studies, protein quality has not. There are many measures
of protein quality. Some are based on feeding studies (e.g. protein effi-
ciency ratio, biological value, or net protein utilization) and others
are based on how well the essential amino acid profile of a particular
food matches a hypothetical perfect protein or the animal’s require-
ments (e.g. chemical score). The latter estimates are appealing in that
amino acid profiles of many foods have been determined and very
extensive effort has been made to define the amino acid requirements
of domestic and laboratory animals [NRC (National Research Coun-
cil) 1995].
The complete amino acid profiles of Chinook salmon (Oncorhyn-
chus tshawytscha) and white-tailed deer (Odocoileus virginianus) fed to
brown bears (Ursus arctos) and American black bears (Ursus americ-
anus) (Hilderbrand et al. 1996; Felicetti et al. 2003) and various diets
composed of corn, wheat, alfalfa, soybean meal, lentils, chicken meal,
pork meat and bone meal and fish meal fed to laboratory rats (Rob-
bins, Felicetti & Florin 2010; current study) were determined at the
University of Missouri Agricultural Experiment Station Chemical
Laboratories. Briefly, acid and alkaline hydrolysates were analysed
using a high-performance liquid chromatographic amino acid ana-
lyzer. Additional amino acid profiles or protein contents of foods not
reported by other investigators were estimated from the compilations
of NRC (1994), Davis et al. (1994), American Casein Co. (Burling-
ton, NJ, USA) and the USDA National Nutrient Database for
Standard Reference, Agricultural Research Service (http://www.nal.
usda.gov/fnic/foodcomp/search/) (see Table S1, Supporting informa-
tion).
The basis for estimating protein quality was to express the concen-
tration of each essential amino acid in the diet as a percent of the diet’s
crude protein (N · 6Æ25) content. This relative concentration of each
amino acid was compared with the estimated dietary requirement of
that amino acid as a percent of the total protein requirement for
growth by laboratory rats (Rattus rattus) (NRC 1995) to determine
which amino acid might be most limiting. Amino acid requirements
for growing laboratory rats were used as the standard for all animals
because (i) the amino acid requirements for wild animals are almost
entirely unknown; (ii) the current and previous study (Robbins, Felic-
etti & Florin 2010) used laboratory rats; and (iii) laboratory rats have
not been heavily selected for meat, milk, or egg production as have
many other domestic animals (e.g. livestock and poultry) and, there-
fore, may be a more appropriate comparison with wild animals.
SELECTING NITR OGEN AND SULPHUR DISCRIMINA-
TION VALUES
D15
N and D34
S values for serum, plasma, whole blood, or red blood
cells were sought for diets that covered the widest possible ranges of
protein quality and quantity, had been fed long enough to ensure diet
to animal equilibration and had been fed by multiple investigators or
Ó 2010 The Authors. Functional Ecology Ó 2010 British Ecological Society, Functional Ecology, 25, 519–526
520 S. T. Florin et al.
3. in various combinations to ensure that the reported isotope discrimi-
nations were reliable. Unfortunately, results on commercial rodent
and poultry diets as well as several other diets could not be used
because of the impossibility of estimating amino acid profiles. Simi-
larly, feeding studies that used pelleted diets were excluded because of
concern about protein damage (Robbins, Felicetti & Florin 2010),
and studies that fed fungi, crustacea, or insects [e.g. mealworms (Ten-
ebrio molitor)] were excluded because much of their nitrogen occurs as
chitin (a nitrogen-containing carbohydrate) (Claridge et al. 1999;
Pearson et al. 2003). For example, van Tets & Hulbert (1999)
estimated that 69% of the nitrogen in mealworms occurred as non-
protein chitin.
TESTING THE INTERACTION BETWEEN PR OT EIN
QUANTIT Y, QUALITY AND D1 5
N
Two approaches were used to test the interactions between protein
quantity and quality in determining D15
N. The first approach was an
indirect test in which the relative amount of the most limiting essential
amino acid was compared with the D15
N for several diet–animal com-
binations used in the current and previous studies (Hobson & Clark
1992; Hilderbrand et al. 1996; Hobson et al. 1996; Ben-David &
Schell 2001; Jenkins et al. 2001; Bearhop et al. 2002; Lesage, Hammill
& Kavacs 2002; Felicetti et al. 2003; Sponheimer et al. 2003; Ogden,
Hobson & Lank 2004; Arneson & MacAvoy 2005; Cherel, Hobson &
Hassani 2005; Robbins, Felicetti & Sponheimer 2005; Podlesak &
McWilliams 2006; Darr & Hewitt 2008; Tsahar et al. 2008; Robbins,
Felicetti & Florin 2010) (see Table S2). If the protein quality hypothe-
sis is valid, D15
N should decrease as the concentration of the most lim-
iting amino acid increases across diets. Similarly, if the protein
quantity hypothesis is valid, D15
N should increase above the relation-
ship determined by protein quality alone once the most limiting
amino acid is no longer the sole determinant of dietary protein turn-
over.
The second approach was a direct test in which foods ranging in
both protein quality and quantity were supplemented with additional
energy to dilute the protein concentration and thereby reduce daily
protein intake. The D15
N and D34
S of animals consuming the energy-
supplemented diets should be less than the non-supplemented diets
when protein quantity becomes important in determining discrimina-
tion. Specifically, we hypothesized that D15
N values for plant-based
diets would be less likely to decrease with energy dilution than ani-
mal-based diets.
Thus, the diets used included fish meal (Brevoortia tyrannus),
chicken meal, pork meat and bone meal, soybean meal, lentils and
wheat because they cover a wide range in both protein quantity and
quality in both plants and animals. All feeds were purchased as single
batches, finely ground and mixed thoroughly to ensure that composi-
tion and isotopic values were constant. Each diet was fed in the undi-
luted form followed immediately by the diluted form to the same 10
rats. The diluted diets were supplemented with nitrogen- and sulphur-
free sucrose, starch and corn oil in the ratio of 5 : 2 : 2 : 1, such that
the protein concentration was reduced by 50%. Further dilution was
not attempted because of concern about creating nutritional deficien-
cies.
Ten male, Sprague–Dawley laboratory rats were used in all feeding
trials. Each feeding trial lasted 21 days to ensure that plasma had
equilibrated with the diet and followed the protocol of Robbins,
Felicetti & Florin (2010). Blood samples were collected in heparinized
tubes at the end of each feeding trial. Plasma was separated, frozen,
and freeze-dried. All rats were fed ad libitum to promote positive
energy and protein balance, weight gain, and therefore minimal tissue
mobilization. Rats were weighed weekly.
ISOTOPIC AND STATISTIC AL ANALYSES
d15
N and d34
S values for diets and freeze-dried plasma were deter-
mined with a continuous flow isotope ratio mass spectrometer (Delta
PlusXP; Thermo Finnigan, Bremen, Germany) at the Washington
State University Stable Isotope Core Laboratory. Mean dietary iso-
tope values were based on the analyses of five samples per diet. Nitro-
gen isotope ratios are reported as per mil (&) relative to atmospheric
nitrogen (d15
N). Sulphur isotope ratios are reported as per mil relative
to Vienna Canon Diablo Troilite by assigning a value of )0Æ3& to
IAEA S-1 silver sulphide. Laboratory reference standards (acetanilide
and keratin for nitrogen and sulfanilimide, IAEA S-2, IAEA SO5,
and IAEA S3 for sulphur) were interspersed throughout each analysis
to ensure maintenance of calibration. Analytical errors (±1 SD) for
the above standards were £0Æ1& for nitrogen and £0Æ4& for sulphur.
Linear and curvilinear least squares regressions were used to model
all relationships (SAS 1998). Differences in slopes of regressions were
tested using small sample t-tests (Kleinbaum & Kupper 1978). ANOVA
was used to test for differences in discrimination between diets.
A P-value of <0Æ05 was considered significant. Means are reported
with ±1 SD.
Results
NITROGEN ISOTOPE RAT IO DISCR IMINATION
Protein quality as defined by the relative methionine concen-
tration accounted for 87–90% of the variation in D15
N when
animals consumed diets that ranged from 6Æ9% to 53Æ8% pro-
tein with methionine concentrations ranging from 1Æ3% to
2Æ6% (Fig. 1, see Tables S1 and S2). The inclusion of the
other sulphur-containing amino acids that can partially sub-
stitute for methionine (i.e. cystine, cysteine and taurine) did
not improve the regressions. The pattern of decreasing D15
N
with increasing protein quality occurred for laboratory rats
consuming a wide range of single-item and mixed diets (Rob-
bins, Felicetti & Florin 2010; current study), non-primate
neonates consuming milk (Robbins 1993; Davis et al. 1994;
Jenkins et al. 2001; Robbins, Felicetti & Sponheimer 2005),
wild and domestic ruminants consuming alfalfa or alfalfa and
corn (Jenkins et al. 2001; Sponheimer et al. 2003; Darr & He-
witt 2008), and yellow-vented bulbuls (Pycnonotus xanthopy-
gos) and yellow-rumped warblers (Dendroica coronata)
consuming mixed diets of casein and bananas (Tsahar et al.
2008) or casein, sugar and olive oil (Podlesak & McWilliams
2006).
However, several diets had either higher or lower discrimi-
nations than predicted by the regression equations of Fig. 1.
For example, lentils containing relatively low quality protein
had a much lower D15
N than predicted by the regressions. At
the other extreme, high-protein meat diets containing rela-
tively high quality protein (e.g. fish, fish meal, chicken meal,
ungulates, and quail) fed to various mammals (Canis latrans,
Halichoerus grypus, Mustela vison, Pagophilus groenlandicus,
Phoca hispida, Phoca vitulina, U. americanus, U. arctos) and
Ó 2010 The Authors. Functional Ecology Ó 2010 British Ecological Society, Functional Ecology, 25, 519–526
Understanding nitrogen and sulphur discrimination 521
4. birds (Calidris alpine pacifica, Catharacta skua, Corvus brac-
hyrhynchos, Falco peregrines, Larus delawarensis) had higher
nitrogen discriminations than predicted from protein quality
alone (Fig. 1, see Tables S1 and S2) (Hobson & Clark 1992;
Hilderbrand et al. 1996; Hobson et al. 1996; Ben-David &
Schell 2001; Bearhop et al. 2002; Lesage, Hammill & Kavacs
2002; Felicetti et al. 2003; Ogden, Hobson & Lank 2004;
Arneson & MacAvoy 2005; Cherel, Hobson & Hassani 2005;
Robbins, Felicetti & Florin 2010). Discriminations for five
of six meat diets averaged 1Æ1 ± 0Æ4& higher (range =
0Æ5–1Æ6&) (Fig. 1) than predicted from protein quality alone.
The exception to this trend occurred when laboratory rats
consumed pork meat and bone meal that contained relatively
low quality protein (Fig. 1, see Tables S1 and S2). Its discrim-
ination (5Æ0 ± 0Æ1&) was similar to what would be predicted
from the more general protein quality regressions of Fig. 1
(5Æ1–5Æ2&).
The protein content of the plant and animal foods used in
the protein dilution study ranged from 12Æ5% to 72Æ0%
(Fig. 2, see Table S2); and protein quality in those foods was
limited by the amino acid methionine, which ranged from
0Æ85% to 2Æ61% of the crude protein (see Tables S1 and S2).
Average daily protein intake was reduced by 48Æ7 ± 3Æ5%
when rats consumed the diluted diets relative to the undiluted
diets. Rats gained weight on all plant-based diets with and
without dilution (1Æ7 ± 1Æ2 g day)1
, range = 0Æ7–3Æ7) and
on five of six animal-based diets (1Æ3 ± 0Æ5 g day)1
, ran-
ge = 0Æ8–2Æ0). The exception was some rats lost weight on
the undiluted pork meat and bone meal ()1Æ0 ± 1Æ5 g day)1
),
but they gained weight on diluted pork meat and bone meal
(1Æ5 ± 0Æ35 g day)1
). However, there was no difference in the
nitrogen discrimination for rats that lost weight when con-
suming the pork meat and bone meal as compared with those
that maintained or gained weight (t = 0Æ58, P = 0Æ59).
Nitrogen discrimination did not decrease with energy dilu-
tion in any of the plant-based diets (lentils, F = 0Æ43,
P = 0Æ52 and soybean meal F = 0Æ40, P = 0Æ54), although
D15
N slightly increased (0Æ17 ± 0Æ17&) when wheat was
diluted (F = 7Æ53, P = 0Æ01) (Figs 1 and 2). The mean differ-
ence in discrimination due to dilution for the three plant diets
was 0Æ06 ± 0Æ13& and did not differ from 0 (t = 0Æ77,
P = 0Æ52). D15
N decreased in two of three meat diets (fish
meal, F = 91Æ4, P < 0Æ0001 and pork meat and bone meal,
F = 34Æ9, P < 0Æ001), but did not decrease when chicken
meal was diluted (F = 1Æ69, P = 0Æ21) even though seven of
the ten rats had lower discriminations when consuming the
diluted diet (Figs 1 and 2).
Fig. 1. The relationship between dietary protein quality as defined by
the limiting amino acid (methionine) and D15
N for the plasma, serum
or red blood cells of laboratory rats consuming various diets of corn,
wheat, alfalfa, lentils, soybean meal, fish meal, pork meat and bone
meal, chicken meal, and their mixtures (Robbins, Felicetti & Florin
2010; current study) and various wild birds and mammals consuming
fish (Hobson & Clark 1992; Hilderbrand et al. 1996; Hobson et al.
1996; Ben-David & Schell 2001; Bearhop et al. 2002; Lesage, Ham-
mill & Kavacs 2002; Felicetti et al. 2003; Cherel, Hobson & Hassani
2005), fish meal (Ogden, Hobson & Lank 2004; Arneson & MacAvoy
2005; Robbins, Felicetti & Florin 2010), quail (Hobson & Clark
1992), ungulates (Hilderbrand et al. 1996; Ben-David & Schell 2001;
Bearhop et al. 2002), alfalfa or alfalfa and corn (Jenkins et al. 2001;
Sponheimer et al. 2003; Darr & Hewitt 2008), non-primate milks
(Davis et al. 1994; Jenkins et al. 2001; Robbins, Felicetti & Sponhei-
mer 2005) and casein-supplemented diets (Podlesak & McWilliams
2006; Tsahar et al. 2008) (Table S2). Although results for vertebrate
flesh with high quality protein (i.e. ungulates, fish meal, fish, chicken
meal and quail) and lentils as the entire diet are plotted, they are not
included in the regressions.
–1
–0·5
0
0·5
1
0·5 1 1·5 2 2·5
ChangeinΔ15NorΔ34S(‰)
Protein quality (methionine content as a % of dietary protein)
Lentils
Soybean meal
Wheat
Fish mealPork meal
Chicken meal
Fig. 2. The effect on nitrogen and sulphur discrimination when diets
composed of fish meal, chicken meal, pork meat and bone meal, soy-
bean meal, lentils and wheat were fed to laboratory rats with and
without dilution. The diluted diets were created by supplementing
each of the above foods with sucrose, starch and corn oil in the ratio
of 5 : 2 : 2 : 1 to reduce protein concentration to half of that in the
undiluted diet. The change in D15
N and D34
S is the difference between
when laboratory rats were fed the diluted diet minus the undiluted
diet.
Ó 2010 The Authors. Functional Ecology Ó 2010 British Ecological Society, Functional Ecology, 25, 519–526
522 S. T. Florin et al.
5. Nitrogen discriminations can be predicted (R2
= 0Æ81,
F ‡ 68Æ4, P < 0Æ0001, Fig. 3) across the breadth of dietary
data by either one of two equations utilizing both protein
quality and quantity:
D15
N ¼ 7Á62 À 2Á11X þ 0Á015Z eqn 1
D15
N ¼ À6Á02 þ 0Á14X þ 0Á015Z eqn 2
where X is protein quality [eqn 1, methionine content as a
per cent of total dietary protein (Fig. 1a) or eqn 2, the rela-
tive deficit of the most limiting amino acid as a per cent of
the requirement for growth by laboratory rats (Fig. 1b)]
and Z is dietary protein content (% of total dietary dry mat-
ter). The equations utilized all data of Table S2 and Fig. 1
with the exception of the values for lentils (see Discussion).
Protein quality accounted for 75% of the variation
(F ‡ 98Æ9, P < 0Æ0001) and protein quantity for 7%
(F = 2Æ5, P = 0Æ12). More complex regressions, such as
curvilinear regressions or linear and curvilinear regressions
with thresholds for a protein quantity effect (e.g. ‡50%),
produced similar overall predictive capabilities (R2
=
0Æ82–0Æ84, F ‡ 78Æ9, P < 0Æ0001) and estimates of the rela-
tive importance of protein quality (74–76%) and quantity
(5–6%) (eqns 1 and 2).
SULPHUR ISOTOPE RATIO DISCRIMINAT ION
Sulphur amino acids accounted for 84 ± 20% of the dietary
sulphur in corn, wheat, alfalfa, soybean meal, fish meal,
chicken meal, and pork meat and bone meal. However, D34
S
was not highly correlated with D15
N (Fig. 4). D34
S did not
change when sucrose, starch and corn oil were added to any
of the six feeds relative to the undiluted diets (mean change in
D34
S values with dilution = 0Æ02 ± 0Æ13, F = 0Æ34–1Æ26,
P = 0Æ08–0Æ77) (Fig. 2). Dietary d34
S accounted for 89% of
the variation in D34
S (see Table S3, Fig. 5). Regressions
between various measures of sulphur amino acid content,
including total sulphur amino acid content, methionine con-
tent, cystine and cysteine content, and methionine to cystine
ratio, had lower correlation coefficients that ranged from
0Æ46 to 0Æ77.
Discussion
Numerous animal and dietary factors have been proposed to
affect nitrogen discrimination by specific tissues. The animal
factors include intake rate, growth rate, metabolic rate, iso-
tope routing, and type of nitrogen excretion (ureotelic or uri-
cotelic), and the dietary factors include protein quality and
quantity (Martinez del Rio et al. 2009; Kelly & Martinez del
Rio 2010; Smith et al. 2010). The animal factors create con-
cern when trying to estimate the assimilated diets of both
ancient and extant animals because they are rarely known.
The current relationships (Fig. 1 and eqns 1 and 2), which
were developed for mammals and birds that were either main-
taining or gaining weight, suggest that most of the variation
in nitrogen discriminations under these conditions is due to
1
3
5
7
1 3 5 7
PredictedΔ15N(‰)
Observed Δ15N (‰)
Y = 0·78 + 0·81X
R2
= 0·81
N = 35
t = 33·8, P < 0·0001
1:1
Fig. 3. The relationship between the observed discriminations of the
diets in Table S2 and their predicted discriminations when solving
eqns 1 and 2 utilizing their respective protein qualities and quantities.
Dashed line is the 1 : 1 relationship between the variables.
Fig. 4. The relationship between nitrogen and sulphur discrimination
for a range of foods fed to laboratory rats (Robbins, Felicetti & Florin
2010; current study) and grizzly bears (Felicetti et al. 2003).
Fig. 5. The relationship between dietary d34
S, plasma or serum d34
S,
and D34
S for diets fed to laboratory rats (Robbins, Felicetti & Florin
2010; current study) and grizzly bears (Felicetti et al. 2003)
(Table S3).
Ó 2010 The Authors. Functional Ecology Ó 2010 British Ecological Society, Functional Ecology, 25, 519–526
Understanding nitrogen and sulphur discrimination 523
6. dietary protein quality and quantity (R2
= 0Æ81–0Æ90). As we
hypothesized, D15
N values for animals consuming plant-
based diets of lower protein quality, even when containing rel-
atively large amounts of protein (e.g. lentils and soybean
meal), were not reduced by dietary energy dilution. D15
N val-
ues for animals consuming two of three high protein meat
diets were reduced when diluted with additional energy,
although the reductions were relatively small in both the dilu-
tion feeding studies (Figs 1 and 2) and in eqns 1 and 2 when
using data from this and other studies.
Nevertheless, the predictive power of the regressions based
on these two variables exceeds that determined by the more
common regressions between dietary d15
N and D15
N, which
explain from 0 to 67% (mean = 40 ± 32%) of the variation
in birds and mammals (Caut, Angulo & Courchamp 2009;
Robbins, Felicetti & Florin 2010). Furthermore, the use of
laboratory rat nutrient standards for growth as a basis for
comparing a wide variety of birds and mammals of various
sizes, gastrointestinal tracts, and productivity suggests that
dietary-induced metabolic relationships determining discrim-
ination are quite conservative.
Lentils, soybean meal, and pork meat and bone meal were
chosen as test foods because of their high protein content but
generally low protein quality. Both of these characteristics
should produce relatively high D15
N values, with the lentil
value being extremely high. For example, the expected D15
N
for lentils based on the regressions of Fig. 1 would have ran-
ged from 7Æ4% to 9Æ8&. However, the D15
N for lentils
(5Æ6 ± 0Æ2&), soybean meal (5Æ7 ± 0Æ1&), and pork meat
and bone meal (5Æ0 ± 0Æ1&) did not exceed 6Æ0&.
In a compilation of 134 D15
N values for various tissues
from mammals, birds, crustacea, insects and fish (Vanderklift
& Ponsard 2003), <4% of the values were above 5Æ5& and
none exceeded 6&. In a more recent compilation of 142 D15
N
values for mammals and birds (Caut, Angulo & Courchamp
2009), only four were above 6&, although three of the four
were incorrectly estimated from Felicetti et al. (2003) and
actually ranged from 4Æ3& to 5Æ8&. Thus, the aggregate of
these observations suggests an upper limit to D15
N of c. 6&
for mammals and birds consuming foods that do not contain
significant amounts of non-protein nitrogen. Therefore, D15
N
estimates produced by eqns 1 and 2 should be capped at a
maximum of 6& unless a particular food–animal combina-
tion is known to produce a higher discrimination.
The regression between dietary d34
S and D34
S has a higher
correlation coefficient than those measured for similar carbon
and nitrogen regressions and, therefore, may be all that is
needed to estimate assimilated diet (Hilderbrand et al. 1996;
Felicetti et al. 2003; McCutchan et al. 2003; Vanderklift &
Ponsard 2003; Robbins, Felicetti & Florin 2010). We hypoth-
esize that this high correlation coefficient occurred in this
study because methionine was the primary, limiting, essential
amino acid in all diets. Therefore, sulphur and the sulphur
amino acids should be highly conserved during animal metab-
olism as demonstrated by the lack of any change in D34
S dur-
ing the dietary dilution study. The relatively low correlation
coefficient (0Æ48) between D34
S and D15
N is similar to earlier
results (0Æ44) for insects and fish (McCutchan et al. 2003),
which suggests a more complex relationship between the two
variables. Presumably, D15
N reflects the metabolism of all
amino acids and varies with both protein quality and quan-
tity, whereas D34
S reflects the metabolism of only sulphur
amino acids. Therefore, the two variables are not directly
related and the lower correlation coefficient should be
expected.
If the above results linking protein quality, protein
quantity, and D15
N are confirmed or refined by further
studies, estimating nitrogen discriminations for omnivores
without detailed knowledge of the animal factors may not
limit accurate estimates of assimilated diet. However,
Fig. 6. Illustration of assimilated diet estimates for an omnivore consuming a two-component diet (plants and animals) using either linear (no
dietary interaction) or curvilinear (metabolically mixed diet with complementary amino acid profiles) solutions. The assumptions were that:
(i) the plant component of the diet had a d15
N signature of )1Æ0&, a protein quality of 1Æ4% methionine and a protein content of 24%, which gave
a discrimination estimate of 5Æ0& (eqn 1); and (ii) the animal component had a d15
N signature of 4Æ0&, a protein quality of 2Æ5% methionine and
a protein content of 77%, which gave a discrimination estimate of 3Æ5&. Intermediate discriminations for the metabolically mixed diets were
determined by solving eqn 1 for various dietary mixtures. Because the discriminations at a given dietary mixture were lower when the two foods
were consumed in a metabolically mixed diet than when there was no dietary interaction, the linear model underestimates the importance of
animal matter and overestimates the importance of plant matter in the diet when the foods were consumed in a metabolically mixed diet. The
maximum error in the assimilated diet estimates for each dietary component in this example was 14%.
Ó 2010 The Authors. Functional Ecology Ó 2010 British Ecological Society, Functional Ecology, 25, 519–526
524 S. T. Florin et al.
7. current limitations to this approach include the lack of: (i)
a broad understanding of amino acid profiles in the wide
range of foods consumed by wild animals and the time
course of their metabolic interaction within the consumer
that will determine if they are complementary or non-
complementary; (ii) an understanding of how mixtures of
protein and non-protein nitrogen (e.g. chitin) in insects,
crustacea, and fungi determine nitrogen discrimination;
and (iii) mixing models in which the discriminations of the
individual foods vary from being independent, additive,
and linear for foods consumed in metabolically distinct
meals to dependent and curvilinear when foods with com-
plementary amino acid profiles are consumed in metaboli-
cally mixed diets (DeGabriel, Foley & Wallis 2002;
Robbins, Felicetti & Florin 2010) (Fig. 6).
This latter point means that discriminations may need to
be predicted parameters in mixing models based on addi-
tional animal, dietary, and temporal inputs rather than the
current a priori estimates. However, investigators working
with piscivores, other carnivores, and herbivores may have a
much easier task in estimating discriminations as many of
these groups do not consume foods that vary as extensively in
protein quality and quantity as do the diets consumed by
some omnivores. Although there may be other diets with
nitrogen discriminations that are outside the bounds of our
current understanding, the equations developed in this study
offer the opportunity to begin developing more complex and
realistic mixing models for omnivores that more accurately
estimate assimilated diets.
Acknowledgements
The project was approved by the Washington State University Institutional
Animal Care and Use Committee (#03762) and funded by the Nutritional Ecol-
ogy Research Endowment and the US Fish and Wildlife Service.
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Received 15 June 2010; accepted 16 September 2010
Handling Editor: Adam Kay
Supporting Information
Additional Supporting Information may be found in the online
version of this article.
Table S1. The essential amino acid requirement for growth by labora-
tory rats (NRC 1995) relative to the same amino acids occurring in
various foods used in the current and previous isotope studies (Hil-
derbrand et al. 1996; Darr & Hewitt 2008; Tsahar et al. 2008; Rob-
bins, Felicetti & Florin 2010). Both the requirement and dietary
amino acid profile are expressed as a per cent of total dietary protein.
The subscripts in the diet columns indicate the deficit occurring for
each amino acid in each food relative to the requirement.
Table S2. Estimated or measured dietary protein characteristics and
nitrogen discrimination of whole blood, plasma, serum, or red blood
cells for the diets fed to a wide range of mammals and birds. Protein
quality estimates are from Table S1 (Supporting information) and
based on laboratory rat amino acid requirements for growth. The
numbers following several mixed diets (e.g. 75:25 or 50:50) refer to the
relative contribution of protein by the two ingredients (Robbins, Fe-
licetti & Florin 2010; current study). The ‘diluted’ diets refer to the
addition of sucrose, starch and corn oil to reduce protein content by
50% while holding protein quality constant.
Table S3. Sulphur isotope values for the diets and plasma of labo-
ratory rats and grizzly bears and the corresponding discrimination
(Felicetti et al. 2003; Robbins, Felicetti & Florin 2010; current
study).
As a service to our authors and readers, this journal provides sup-
porting information supplied by the authors. Such materials may be
re-organized for online delivery, but are not copy-edited or typeset.
Technical support issues arising from supporting information (other
than missing files) should be addressed to the authors.
Ó 2010 The Authors. Functional Ecology Ó 2010 British Ecological Society, Functional Ecology, 25, 519–526
526 S. T. Florin et al.