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• Hysterectomy is the surgical removal of the uterus. It
may also involve removal of the cervix, ovaries,
fallopian tubes and other surrounding structure.
•
•
•
•
•

Type of hysterectomy
Shape of uterus
Endometrium
Cervix
Myomas
• Hysterectomy, in the literal sense of the word,
means merely removal of the uterus. However other
organs such as ovaries, fallopian tubes and the
cervix are very frequently removed as part of the
surgery.
: complete removal
of the uterus, cervix, upper vagina, and parametrium
Indicated for cancer.
: Complete removal of
the uterus and cervix, with or without oophorectomy.
: removal of the
uterus, leaving the cervix.
•
•
•
•

The uterus is a dense organ.
Usually cone biopsies is received.
To allow fixation,the cervix is removed.
Uterus is opened by a
just leaving the
two halves in contact at the fundus.
• The anterior and posterior sections are then ‘toastracked’ by
apart,allow fixative to
enter.
• A total of sixty samples from human breast,
uterus, liver, skin and abdominal fat were
fixed for 8; 24
with
• Volume to tissue ratios of
and
and manually processed with
isopropyl alcohol and mineral oil mixtures.
• All the slides prepared were suitable for
diagnostic purpose.
• The
differences
between the samples was not statistically
significant for the different fixation volume
ratios tested, but the differences between
fixation periods and tissues types were.
•

48 hours are the optimum fixation
period..
• Neither the time and volume ratio
combinations affected the pH of NBF
• Fixing tissues with a ratio of NBF
volume to tissue volume of 2:1 for 48
hours at 20-22°C was enough to assure
• proper fixation and infiltration of the
tested tissues.
Findings indicate that the specimen should be placed in
fixative as soon as possible after excision and before
opening to ensure minimum distortion of the specimen.
: surgical removal of uterus

: lateral incision of uterus in which
tissue thickness should be 1-1.5cm.
: whole uterus is put in a jar
containing neutral buffer formalin for 8-24 hours or 48
hours at 20 to 22°C.
•
•

:
:Specimen should be placed in a fixative
immediately to prevent distortion after excision.
Fixation of uterus after whole hysterectomy

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Fixation of uterus after whole hysterectomy

  • 1.
  • 2.
  • 3. • Hysterectomy is the surgical removal of the uterus. It may also involve removal of the cervix, ovaries, fallopian tubes and other surrounding structure. • • • • • Type of hysterectomy Shape of uterus Endometrium Cervix Myomas
  • 4. • Hysterectomy, in the literal sense of the word, means merely removal of the uterus. However other organs such as ovaries, fallopian tubes and the cervix are very frequently removed as part of the surgery. : complete removal of the uterus, cervix, upper vagina, and parametrium Indicated for cancer. : Complete removal of the uterus and cervix, with or without oophorectomy. : removal of the uterus, leaving the cervix.
  • 5.
  • 6. • • • • The uterus is a dense organ. Usually cone biopsies is received. To allow fixation,the cervix is removed. Uterus is opened by a just leaving the two halves in contact at the fundus. • The anterior and posterior sections are then ‘toastracked’ by apart,allow fixative to enter.
  • 7. • A total of sixty samples from human breast, uterus, liver, skin and abdominal fat were fixed for 8; 24 with • Volume to tissue ratios of and and manually processed with isopropyl alcohol and mineral oil mixtures. • All the slides prepared were suitable for diagnostic purpose. • The differences between the samples was not statistically significant for the different fixation volume ratios tested, but the differences between fixation periods and tissues types were.
  • 8. • 48 hours are the optimum fixation period.. • Neither the time and volume ratio combinations affected the pH of NBF • Fixing tissues with a ratio of NBF volume to tissue volume of 2:1 for 48 hours at 20-22°C was enough to assure • proper fixation and infiltration of the tested tissues.
  • 9. Findings indicate that the specimen should be placed in fixative as soon as possible after excision and before opening to ensure minimum distortion of the specimen.
  • 10. : surgical removal of uterus : lateral incision of uterus in which tissue thickness should be 1-1.5cm. : whole uterus is put in a jar containing neutral buffer formalin for 8-24 hours or 48 hours at 20 to 22°C. • • : :Specimen should be placed in a fixative immediately to prevent distortion after excision.