Cloud BioLinux provides a customizable open source bioinformatics computing environment on cloud platforms for genomics research. As sequencing technologies become more accessible, individual labs will need to perform their own analysis but may lack dedicated infrastructure. Cloud BioLinux addresses this need by offering pre-configured virtual machines with over 100 bioinformatics tools on Amazon EC2, allowing researchers to perform large-scale analysis using only a web browser. Developers can extend Cloud BioLinux by specifying additional software configurations using a framework based on Python fabric scripts. The project aims to expand its community and tool offerings to better support sequencing centers and individual researchers.