This document provides an introduction to using a simulation called the Natural Selection Simulation to explore variation and distribution in populations. It describes the key organisms in the simulation - thornpalms, ostrilopes, and carnithons - and how their traits can be adjusted. It also explains how to use the simulation's features like turning populations on/off, zooming in on organisms, and changing trait levels and variation using sliders. Students will complete missions in the simulation by adjusting these sliders. The document concludes with an activity where students build histograms out of cubes to represent different levels of variation and distributions in populations.