This document discusses blood glucose estimation, including indications for testing such as diabetes and disorders of carbohydrate metabolism. It describes several methods for glucose estimation including reduction, condensation, and enzymatic methods. The glucose oxidase-peroxidase (GOD-POD) enzymatic method is explained in detail. Normal ranges for fasting, random, and post-prandial glucose are provided. Causes of hyperglycemia and hypoglycemia are listed. The importance of using fluoride bulbs to obtain stable glucose readings is explained.