Argo floats (http://www.argo.ucsd.edu/) measure ocean temperature and salinity in the upper 2000 meters on a global scale. Spatial statistics provides the tools for reconstructing the full temperature and salinity fields based on sparse point observations from the floats. The large size and complex spatio-temporal dependence structure of the Argo data set mean that state-of-the-art statistical techniques are needed for efficient reconstructions. The goal of this project is to learn to explore, visualize, model and spatially interpolate subsets of Argo data using R. The preliminary plan is to use the "fields" package and to see how far we can push the tools there when reconstructing regional ocean temperature fields.
Group members: Ahmet Hatip, Alex Hayes, Alexa Maxwell, Joseph Struzeski, Ingrid Tchkaoua, Wenbo Wang
Argo floats (http://www.argo.ucsd.edu/) measure ocean temperature and salinity in the upper 2000 meters on a global scale. Spatial statistics provides the tools for reconstructing the full temperature and salinity fields based on sparse point observations from the floats. The large size and complex spatio-temporal dependence structure of the Argo data set mean that state-of-the-art statistical techniques are needed for efficient reconstructions. The goal of this project is to learn to explore, visualize, model and spatially interpolate subsets of Argo data using R. The preliminary plan is to use the "fields" package and to see how far we can push the tools there when reconstructing regional ocean temperature fields.
Group members: Ahmet Hatip, Alex Hayes, Alexa Maxwell, Joseph Struzeski, Ingrid Tchkaoua, Wenbo Wang
An Introduction to the Environment Agency extreme offshore wave, water level ...Stephen Flood
An Introduction to the Environment Agency extreme offshore wave, water level and wind conditions data sets, transformed to nearshore for events covering up to the 10000 year extreme coastal event, available to all for use in local studies.
Presented at the DHI UK Symposium 2018.
A study on the lower tropospheric thermal inversion over the arabian sea usin...ieeepondy
A study on the lower tropospheric thermal inversion over the arabian sea using radiosonde and iasi data
+91-9994232214,7806844441, ieeeprojectchennai@gmail.com,
www.projectsieee.com, www.ieee-projects-chennai.com
IEEE PROJECTS 2016-2017
-----------------------------------
Contact:+91-9994232214,+91-7806844441
Email: ieeeprojectchennai@gmail.com
Summary of key findings of "Climate Change 2013: The Physical Science Basis, Working Group I contribution to the IPCC 5th Assessment Report" by Matt Collins, University of Exeter, UK
SICCME open session, 17 September 2014, ICES Annual Science Conference, A Coruña, Spain
International Journal of Engineering Research and Applications (IJERA) is an open access online peer reviewed international journal that publishes research and review articles in the fields of Computer Science, Neural Networks, Electrical Engineering, Software Engineering, Information Technology, Mechanical Engineering, Chemical Engineering, Plastic Engineering, Food Technology, Textile Engineering, Nano Technology & science, Power Electronics, Electronics & Communication Engineering, Computational mathematics, Image processing, Civil Engineering, Structural Engineering, Environmental Engineering, VLSI Testing & Low Power VLSI Design etc.
An Introduction to the Environment Agency extreme offshore wave, water level ...Stephen Flood
An Introduction to the Environment Agency extreme offshore wave, water level and wind conditions data sets, transformed to nearshore for events covering up to the 10000 year extreme coastal event, available to all for use in local studies.
Presented at the DHI UK Symposium 2018.
A study on the lower tropospheric thermal inversion over the arabian sea usin...ieeepondy
A study on the lower tropospheric thermal inversion over the arabian sea using radiosonde and iasi data
+91-9994232214,7806844441, ieeeprojectchennai@gmail.com,
www.projectsieee.com, www.ieee-projects-chennai.com
IEEE PROJECTS 2016-2017
-----------------------------------
Contact:+91-9994232214,+91-7806844441
Email: ieeeprojectchennai@gmail.com
Summary of key findings of "Climate Change 2013: The Physical Science Basis, Working Group I contribution to the IPCC 5th Assessment Report" by Matt Collins, University of Exeter, UK
SICCME open session, 17 September 2014, ICES Annual Science Conference, A Coruña, Spain
International Journal of Engineering Research and Applications (IJERA) is an open access online peer reviewed international journal that publishes research and review articles in the fields of Computer Science, Neural Networks, Electrical Engineering, Software Engineering, Information Technology, Mechanical Engineering, Chemical Engineering, Plastic Engineering, Food Technology, Textile Engineering, Nano Technology & science, Power Electronics, Electronics & Communication Engineering, Computational mathematics, Image processing, Civil Engineering, Structural Engineering, Environmental Engineering, VLSI Testing & Low Power VLSI Design etc.
A Computational Study Of Heat Transfer Due To The Inlet Oscillating Condition...theijes
Heat transferis numerically investigated in aconfined oscillating laminar slot jet. The inlet velocity profile is uniform, and oscillating with an angle φ (in radians) as follows:휑 = 휑푚푎푥 ∗ 푠푖푛(2휋푓푡).φmax is the maximum jet angle, and f is the frequency of oscillation. The height-to-jet-width ratio was set to 5, the fluid’s Prandtl number is 0.74 and Reynolds number was fixed at 250.Strouhal’s numberSt, which is the other dimensionless group characterizing this problem, was varied in the range 0.05<st><0.75.The jet hydraulic diameter (2w), was used in the definition of both Strouhal and Reynolds numbers. φmax was defined, based on a solid finding presented later in this paper. At St=0.4, and 0.5, a modest enhancement of heat transfer was noticed in the stagnation region, when compared to a steady jet
DSD-INT 2019 Lake Kivu - 3D hydrodynamic modelling of a deep and strongly str...Deltares
Presentation by Wouter Kranenburg, Deltares, at the Delft3D - User Days (Day 2: Hydrodynamics), during Delft Software Days - Edition 2019. Tuesday, 12 November 2019, Delft.
Peters et al_a_search_for_large-scale_effects_of_ship_emissions_on_clouds_and...www.thiiink.com
Introduction
Ship tracks are widely seen as one of the most prominent
manifestations of anthropogenic aerosol indirect effects
(AIEs), or the change in cloud properties by anthropogenic
aerosols serving as cloud condensation nuclei. A very uncertain
and scientifically interesting question, however, is about
the climatically relevant large-scale forcing by AIEs due to
ship emissions.
In the past decades, a whole suite of AIE-hypotheses has
been put forward of which the “Twomey-effect”, or first AIE,
is the most prominent. For this effect, an increase in available
cloud condensation nuclei (CCN) eventually leads to
more and smaller cloud droplets if the liquid water content of
the respective cloud remains constant. More cloud droplets
increase the total droplet surface area by which the cloud
albedo is enhanced; an effect which was put into the general
context of anthropogenic pollution by Twomey (1974). Other
AIE-hypotheses include effects on cloud lifetime (Albrecht,
1989; Small et al., 2009) or cloud top height (Koren et al.,
2005; Devasthale et al., 2005). Especially the latter hypotheses
are far from being verified (e.g. Stevens and Feingold,
2009). In total, AIEs are subject to the largest uncertainties
Published by Copernicus Publications on behalf of the European Geosciences Union.
5986 K. Peters et al.: Aerosol indirect effects from shipping emissions
of all radiative forcing (RF) components of the Earth System,
when it comes to assessing human induced climate change
(Forster et al., 2007). However, there exists broad consensus
that on global average, AIEs have a cooling effect on the
Earth System with the most recent multi-model estimate being
−0.7 ± 0.5 W m−2
(Quaas et al., 2009).
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Esters, Leonie: Evaluation of a turbulence-based description of the air-water gas transfer velocity
1. Evaluation of a turbulence-based description of
the air-water gas transfer velocity
Leonie Esters, Erik Sahl´ee, Erik Nilsson, Lucia Guti´errez-Loza,
Anna Rutgersson
ICOS Science Conference 2020
15.09.2020
Leonie Esters Turbulence-based description of the air-water gas transfer velocity 1
2. Turbulence and Air-Sea Gas Transfer
• The gas transfer velocity k
is usually parametrised as
function of wind speed
• There are attempts to
relate k directly to
near-surface turbulence
⇒ k = A · ( ν)1/4 Sc−n
(Lamont and Scott, 1970)
: dissipation rate of TKE
ν: viscosity of water
Sc: Schmidt number
n: Sc exponent
1/2 ≤ n ≤ 2/3
A: proportionality coefficient
0 2 4 6 8 10 12 14 16
0
20
40
60
80
100
120
Wanninkhof (1992)
Wanninkhof + Mc Gillis (1999)
Liss + Merlivat (1986)
k660
[cmhr
−1
]
u
10
[m s−1
]
Leonie Esters Turbulence-based description of the air-water gas transfer velocity 2
3. 5-beam Acoustic Doppler Current Profiler (ADCP)
Simpson et al. 2001
• ADCPs measure the 3D current
field
• They send sound waves, which are
reflected by particles in the water
• The particles velocity is
determined using the Doppler
effect
• Measurements of:
• water current speed
• water turbulence
• wave characteristics
Leonie Esters Turbulence-based description of the air-water gas transfer velocity 3
4. Deployment in lake Erken
Leonie Esters Turbulence-based description of the air-water gas transfer velocity 4
5. Observations 2018
• 21 days of data
• No reliable
observations of k
• Waves follow the
wind
• Deepening of the
mixing layer depth
(XLD) coincide with
increased winds and
waves
Leonie Esters Turbulence-based description of the air-water gas transfer velocity 5
6. Observations
• 73 days of data
• No observations of k
• Waves follow the
wind
• Deepening of the
XLD coincide with
increased winds,
waves, and positive
buoyancy flux
Leonie Esters Turbulence-based description of the air-water gas transfer velocity 6
7. Surface turbulence versus wind speed
• Total of 94 days
of data
• Filled circles:
2018,
open circles:
2019
• Linear relation
between surface
turbulence and
wind speed
⇒ Turbulence is
wind-driven
-9.5 -9 -8.5 -8 -7.5 -7 -6.5 -6
log10( 05
) [m2
s-3
]
0
2
4
6
8
10
12
14
u10
[ms-1
]
Leonie Esters Turbulence-based description of the air-water gas transfer velocity 7
8. Relating surface turbulence to k
• Wind-speed based
parametrisation for
k by Cole and
Caraco (1998):
k600 = 2.07+0.215u1.7
10
⇒ turbulence-based
model explains the
gas transfer velocity
calculated following
Cole and Caraco
(1998).
100
101
A Sc-n
( 05
)1/4
[cm hr-1
]
100
101
k600
CC
[cmhr-1
]
A = 1.3
R2
= 0.797
2
4
6
8
10
12
14
u10[ms-1
]
Leonie Esters Turbulence-based description of the air-water gas transfer velocity 8
9. Outlook: ¨Ostergarnsholm
• At the moment, the ADCP is deployed in
the footprint of the flux tower on
¨Ostergarnsholm Island in the Baltic Sea.
• There, we have observations of k.
⇒ Surface turbulence can be directly related
to the measured k.
Leonie Esters Turbulence-based description of the air-water gas transfer velocity 9