Enzyme engineering involves modifying enzymes to enhance their activity and utility by changing their amino acid sequences. This field has significant industrial applications, optimizing processes in pharmaceuticals, biofuels, and fine chemicals by increasing enzyme stability, specificity, and catalytic efficiency. Recent advancements include directed evolution techniques, which have led to notable increases in production yields and enzyme performance.

1. Enzyme Engineering
And It’s Industrial
Application
By
PREMKUMAR SAKTHIVEL
MSC BIOCHEMISTRY
D24MBC00022

2. What is enzyme engineering
Enzymes are bio-catalyzers of protein origin which allow
great number of biological reactions. Engineering these
molecules for better beneficial features are commonly
known as Enzyme engineering
Improvement in the activity and usefulness of an existing
enzyme or creation of a new enzyme activity by making
suitable changes in its amino acid sequence is called
Enzyme Engineering

3. Enzyme
Enzymes can range in size from 62
amino acid residues to over 2,500
residue
The active site of an enzyme is
made up of the catalytic site and
the binding site. The catalytic site
is where catalysis occurs, and the
binding site orients substrates.

4. Objectives/Advantages

5. Enzyme Engineering Techniques
°Rational design
°Directed Evolution
° Semi Rational
What’s difference
between Science
And Engineering

6. Rational design and Directed Evolution

7. Semi rational design
Directed evolution involves an extensive, time-consuming
screening and selection process.Rational design requires the
knowledge of the amino acid sequence of the particular
enzyme,along with the three-dimensional information. A
combination of both the strategies, that is,directed evolution
and rational design, leads to development of a novel
approach to over-come these drawbacks, and to improve the
applications of the enzyme

8. Industrial Applications
Enzyme engineering has improved industrial processes by enhancing enzyme
activity, specificity, and stability through directed evolution and computational
tools. Engineered enzymes increase production, reduce costs, and enhance
sustainability in industries like pharmaceuticals, biofuels, and fine chemicals.
Thermo-stability is crucial for industrial applications, and engineered enzymes
exhibit increased tolerance to temperature and organic solvents. High-
throughput screening efficiently identifies improved enzymes with enhanced
catalytic activity and specificity. Enzyme engineering strategies create enzymes
with novel catalytic functions, enabling non-natural reactions. Multi-enzyme
processes offer advantages like easy process control, high reaction rates, and
low toxic by-products.

9. Industrial applications
Directed evolution has led to significant improvements, such as a 10-fold
increase in production of enantiopure hexanediol using alcohol dehydrogenase,
and a 13-fold increase in production of intermediate compounds for atorvastatin
synthesis using glucose dehydrogenase. Additionally, lipase half-life was
increased 500-fold through directed evolution, and phenylalanine ammonia
lyase showed a 15-fold increase in catalytic activity.
Computational modeling continues to aid enzyme engineering and predict
enzyme behavior, while NMR experiments will help understand enzyme
flexibility and function. Multi-enzyme processes will become increasingly
important for industrial applications, offering a promising future for enzyme
engineering.