This document discusses seed quality assurance in India. It makes three key points:
1) Seed quality is essential for higher agricultural productivity and farmer incomes. Factors like genetic purity, physical purity, germination and health are important for quality seeds.
2) Ensuring quality requires cooperation across the seed industry, including variety maintenance, quality production and testing, packaging, and storage. Precise testing is needed using methods like tetrazolium staining.
3) Steps are needed to improve quality assurance, including updating standards, building capacity through training, enhancing laboratory infrastructure, and rationalizing certification norms. Collaboration is important to assure farmers receive high quality seeds.
Varietal identificaton through grow-out test and ElectrophoresisNSStudents
The Presentation is prepared by the N.S Institution of science, Markapur.
It consists of a basic introduction related to Varietal identificaton through grow-out test and Electrophoresis.
Variability in seed testing results, factors affecting the variability, application and use of tolerance tables and seed standards and Sequential sampling
Varietal identificaton through grow-out test and ElectrophoresisNSStudents
The Presentation is prepared by the N.S Institution of science, Markapur.
It consists of a basic introduction related to Varietal identificaton through grow-out test and Electrophoresis.
Variability in seed testing results, factors affecting the variability, application and use of tolerance tables and seed standards and Sequential sampling
As, seed is an key aspect for farmers and grower or producers, thus supply of quality seed and maintenance of seed standards became a matter of core importance. And seed testing in this sector plays a major role in describes procedures intended to characterize the physiological, genetic, and physical attributes of seed and enable informed decisions during research and development, seed production, and along supply chains and trade.
Here is an presentation elaborating about the seed quality testing and parameter involved.
Pearl millet breeding for non-conventional traits and ecologies in South Asia...ICRISAT
Following the principal of demand driven research, key priority areas identified for SA program in Consultation meetings with partners held in 2009, 2011 and 2013 were: blast disease, forage, and heat tolerance. Breeding materials comprising of hybrid parents and germplasm accessions having multi-pathotype resistance to blast were identified, and were utilized in the development of new Blast Resistant breeding materials. Multi-year and multi-location screening of breeding materials were done in high heat stress ecology (air temperatures of >42oC) during summers of 2009-2015 and stable sources of heat tolerance were identified, and new wave of breeding materials have been generated. On forage research, new high biomass materials were identified and utilized to generate hybrids and OPVs providing green forage yields of 45-55 tons ha-1. With the objective of expanding pearl millet hybrid technology to Eastern and Southern African (ESA) region, hybrids were evaluated in ESA region between 2012-2015 which led to identification of some promising hybrids which out yielded local checks by 20-40% in grain yield.
Seed marks the beginning of each plant production and therefore
ensuring its quality is the priority of modern seed science and a prerequisite
for obtaining high yields of all plant species. Determination of seed quality
and its viability indicates what seed lots can be placed onto the market, and
for that reason it is very important to have reliable methods and tests to be
used for seed quality and seed vigour testing
The use of the term cisgenesis is an attempt to distinguish GM plants or other organisms produced in this way from transgenics that is GM plants that contain DNA from unrelated organisms. Schouten et al. (2006) introduced the term cisgenesis and defined cisgenesis as the modification in the genetic background of a recipient plant by a naturally derived gene from a cross compatible species including its introns and its native promoter and terminator flanked in the normal sense orientation. Since cisgenes shared a common gene pool available for traditional breeding the final cisgenic plant should be devoid of any kind of foreign DNA viz., selection markers and vector- backbone sequences. Sometimes the word cisgenesis is also referred to as Agrobacterium-mediated gene transfer from a sexually compatible plant where only the T-DNA borders may be present in the recipient organism after transformation (EFSA, 2012). The cisgenesis precludes linkage drag, and hence, prevents hazards from unidentified hitch hiking genes (Schouten, and Jacobsen, 2008). Compared to transgenesis, one of the disadvantages shared by cisgenesis is that characters outside the sexually compatible gene pool cannot be introduced. Furthermore, development of cisgenic crops involves extraordinary proficiency and time compared to transgenic crops. Therefore, the required genes or fragments of genes may not be readily accessible but have to be isolated from the sexually compatible gene pool (Holme et al., 2013).
On 16 February 2012, European Food Safety Authority (EFSA, 2012) reported the detail study concerning the safety aspects of cisgenic plants and validated that cisgenic plants are secure to be used in terms of environment, food and feed, similar to the traditionally bred plants. However, the present GMO regulation keeps the cisgenic micro-organisms out from its supervision. The first scientific statement of bringing forth a true plant obtained by cisgenic approach was reported in apple through the insertion of the internal scab resistance gene HcrVf2 influenced by their own regulatory genes into the cultivar Gala, a scab susceptible cultivar (Vanblaere et al., 2011). Barley with improved phytase activity was produced successfully by Holme et al. 2011, through cisgenic approach. Late blight resistant potatoes have developed by cisgene stacking of R- gene (jo et al., 2014).
Guidelines for the Conduct of Tests for DUS On Chilli (Hot Pepper), Bell (Sw...kartoori sai santhosh
Guidelines for the Conduct of Tests for Distinctiveness, Uniformity and Stability On
Chilli (Hot Pepper), Bell (Sweet) Pepper and Paprika(Capsicum annuum L.)
International Winter Wheat Improvement Program: breeding strategies and meth...ICARDA
A. Morgunov (CIMMYT-Turkey)
B. Akin (CIMMYT-Turkey),
Y. Kaya (B. Dagdas International Agric. Research Institute, Turkey)
M. Keser (ICARDA-Turkey)
K. Nazari (ICARDA-Syria)
Z. Mert (Central Field Crop Research Institute, Turkey),
R. Sharma (ICARDA-Uzbekistan)
T. Wuletaw (ICARDA-Syria)
Role of biotechnology in enhancing fruit crop production and qualityankit gawri
It was evident that developed biotechnological approaches have the potential to enhance the yield, quality, and shelf-life of fruits and vegetables to meet the demands of the 21st century. However, the developed biotech approaches for fruits and vegetables were more of academic jargon than a commercial reality
As, seed is an key aspect for farmers and grower or producers, thus supply of quality seed and maintenance of seed standards became a matter of core importance. And seed testing in this sector plays a major role in describes procedures intended to characterize the physiological, genetic, and physical attributes of seed and enable informed decisions during research and development, seed production, and along supply chains and trade.
Here is an presentation elaborating about the seed quality testing and parameter involved.
Pearl millet breeding for non-conventional traits and ecologies in South Asia...ICRISAT
Following the principal of demand driven research, key priority areas identified for SA program in Consultation meetings with partners held in 2009, 2011 and 2013 were: blast disease, forage, and heat tolerance. Breeding materials comprising of hybrid parents and germplasm accessions having multi-pathotype resistance to blast were identified, and were utilized in the development of new Blast Resistant breeding materials. Multi-year and multi-location screening of breeding materials were done in high heat stress ecology (air temperatures of >42oC) during summers of 2009-2015 and stable sources of heat tolerance were identified, and new wave of breeding materials have been generated. On forage research, new high biomass materials were identified and utilized to generate hybrids and OPVs providing green forage yields of 45-55 tons ha-1. With the objective of expanding pearl millet hybrid technology to Eastern and Southern African (ESA) region, hybrids were evaluated in ESA region between 2012-2015 which led to identification of some promising hybrids which out yielded local checks by 20-40% in grain yield.
Seed marks the beginning of each plant production and therefore
ensuring its quality is the priority of modern seed science and a prerequisite
for obtaining high yields of all plant species. Determination of seed quality
and its viability indicates what seed lots can be placed onto the market, and
for that reason it is very important to have reliable methods and tests to be
used for seed quality and seed vigour testing
The use of the term cisgenesis is an attempt to distinguish GM plants or other organisms produced in this way from transgenics that is GM plants that contain DNA from unrelated organisms. Schouten et al. (2006) introduced the term cisgenesis and defined cisgenesis as the modification in the genetic background of a recipient plant by a naturally derived gene from a cross compatible species including its introns and its native promoter and terminator flanked in the normal sense orientation. Since cisgenes shared a common gene pool available for traditional breeding the final cisgenic plant should be devoid of any kind of foreign DNA viz., selection markers and vector- backbone sequences. Sometimes the word cisgenesis is also referred to as Agrobacterium-mediated gene transfer from a sexually compatible plant where only the T-DNA borders may be present in the recipient organism after transformation (EFSA, 2012). The cisgenesis precludes linkage drag, and hence, prevents hazards from unidentified hitch hiking genes (Schouten, and Jacobsen, 2008). Compared to transgenesis, one of the disadvantages shared by cisgenesis is that characters outside the sexually compatible gene pool cannot be introduced. Furthermore, development of cisgenic crops involves extraordinary proficiency and time compared to transgenic crops. Therefore, the required genes or fragments of genes may not be readily accessible but have to be isolated from the sexually compatible gene pool (Holme et al., 2013).
On 16 February 2012, European Food Safety Authority (EFSA, 2012) reported the detail study concerning the safety aspects of cisgenic plants and validated that cisgenic plants are secure to be used in terms of environment, food and feed, similar to the traditionally bred plants. However, the present GMO regulation keeps the cisgenic micro-organisms out from its supervision. The first scientific statement of bringing forth a true plant obtained by cisgenic approach was reported in apple through the insertion of the internal scab resistance gene HcrVf2 influenced by their own regulatory genes into the cultivar Gala, a scab susceptible cultivar (Vanblaere et al., 2011). Barley with improved phytase activity was produced successfully by Holme et al. 2011, through cisgenic approach. Late blight resistant potatoes have developed by cisgene stacking of R- gene (jo et al., 2014).
Guidelines for the Conduct of Tests for DUS On Chilli (Hot Pepper), Bell (Sw...kartoori sai santhosh
Guidelines for the Conduct of Tests for Distinctiveness, Uniformity and Stability On
Chilli (Hot Pepper), Bell (Sweet) Pepper and Paprika(Capsicum annuum L.)
International Winter Wheat Improvement Program: breeding strategies and meth...ICARDA
A. Morgunov (CIMMYT-Turkey)
B. Akin (CIMMYT-Turkey),
Y. Kaya (B. Dagdas International Agric. Research Institute, Turkey)
M. Keser (ICARDA-Turkey)
K. Nazari (ICARDA-Syria)
Z. Mert (Central Field Crop Research Institute, Turkey),
R. Sharma (ICARDA-Uzbekistan)
T. Wuletaw (ICARDA-Syria)
Role of biotechnology in enhancing fruit crop production and qualityankit gawri
It was evident that developed biotechnological approaches have the potential to enhance the yield, quality, and shelf-life of fruits and vegetables to meet the demands of the 21st century. However, the developed biotech approaches for fruits and vegetables were more of academic jargon than a commercial reality
Seed treatment by Muhammed Aslam COH,ThrishurAslam Muhammed
This is a small attempt just to introduce diffenrent types of seed treatments as well as special treatments for the better germination ,vigour and survival of the crops under various adverse climatic conditions..
ICRISAT Global Planning Meeting 2019: Research Program – Asia by Dr Pooran Ga...ICRISAT
Refining Product Concepts and ensuring alignment of Crop Breeding efforts to Product Concepts and Modernization of crop improvement programs to accelerate genetic gain.
* To have a better understanding of cassava as a crop
* To identify key traits for characterization
* Phenotyping skills developed for key traits
* To identify and follow good principles for hybridization work (from parents, crosses, data collection, seed collection storage, delivery and planting)
* To demonstrate unique processes with different trials
* Biotech tools and delivery
* Team work and collaboration
*
PRE AND POST HARVEST MANAGEMENT OF SEEDS AMONG HORTICULTURAL CROPSAjai Tiwari
This presentation was delivered at ICAR - Directorate of Seed Research, Mau on 25.12.2015 a in a Training on "Pre and post harvest management of seed crop" for staff of National Seed Corporation Ltd (NSC).
Perspectives and Challenges of Phenotyping in Crop Improvement. - Copy.pptxRonikaThakur
Plant breeding programmes have been supplemented with the rapid advancements in modern technology. But these cannot be exploited fully until a précised phenotypic data is available which can bridge the gap between Genotype and Environment.
So, this presentation is made to have an overview how the advanced high throughput phenotyping platforms are playing a crucial role in the crop improvement.
Use of advanced genomics in determining appropriate breed types for smallhold...
Dr. malvika dadlani
1. Seed Quality Assurance
Malavika Dadlani
Joint Director (Res)
IARI, New Delhi
Indian Seed Congress 2013
2. A Good Seed
• Variety Performance
• Seed quality
are the two most important criteria on
which rests the reputation of a seed
company
3. Why Quality Seed
• Seed is the basic input for
higher productivity (15 -
20%)
• Seed is the natural delivery
system for enhancing
productivity, profitability and
resource use efficiency
• Improves
income, employment and
livelihood
4. Seed Quality
Genetic Purity
Physical Purity
Germination
High seed vigour
Good seed health
-Dr. M.S. Swaminathan
5. Seed quality assurance
“Seed quality assurance" implies that
agencies charged with maintenance of
seed quality cooperate with and support
other areas of seed industry stakeholders
to assure quality products to the end
users. (M Larinde, FAO)
6. Rationale
• Ensure that the best quality seeds are
produced and sold to farmers
• Prevent the spread of weed, pests and
diseases
• Meet consumer demands for specified
qualities (and traits)
• Cater to the needs of specialized farming,
including mechanization
• Provide basis for healthy competition
among seed traders
7. Steps in QA
• Pre-production Variety maintenance
• Production of quality seed
• Processing to obtain quality seed
• Precision in post-production testing of
seed quality
• Seed quality enhancement
• Packaging and storage to retain seed
quality
9. Q2 Analyser
http://www.astec-global.com/seed-analyser
Works on dry seeds and a
non-destructive method. But
needs to modify the design
To suit large no. of samlpes
10. Seed Germination & Seed Viability
• TZ introduced by ISTA during 1960s
• Though labour and time intensive for
seed preparation & evaluation, still a
valuable test for the industry
As per ISTA, if 5 or > 5% seeds are FU,
TZ test is a must
TZ test is simple and anyone can do it!
12. Genetic and Trait Purity
• It is an important tool for variety
maintenance as well as genetically pure
commercial seed production,both in case
of an OPV or hybrid variety.
• Can be performed at different levels:
based on field grow-out, lab tests,
molecular tests etc.
14. Fingerprints and DNA fingerprints
Fingerprints are
unique to each
individual
DNA fingerprints are unique
to each individual
The probability of two
unrelated individuals having
identical DNA fingerprints is
much lower than that of their
fingerprints
Sir Alec Jeffreys, Nature, 1984
15. DNA fingerprinting technology
- Humans Vs Plant varieties
Human Cultivars
• One species, races??? • Multitude of species, subsp, races
• Similarity in requirements • Diverse requirements
• All out breeding • Veg. propagation to crossbred
• One individual, one profile • Diverse genetic composition:
purelines, inbreds, O/P, hybrids,
composites, ---
• Strong to weak substructure
• Subtle substructure, varied
• Common alleles across groups
• Allelic specificity in races
• Applications: identity, purity,
• Applications: identity,
relatedness, genetic makeup
paternity
• Techniques: SSR, SNP, AFLP….?
• Techniques: SSR, SNP
16. Status of science VNTR-RFLP
• Which technique to use!
• Accessibility of technology
RAPD
• Applicability
• Robustness
• Repeatability
• Ambiguities in profiles
ISSR
• Cost
• Sample throughput Sequence/SNP
• Automation ------
RAPD SSR
VNTR
17. STMS markers for hybrid seed purity testing in cotton
Rana et al…..
19. Cost considerations in purity testing
• Present automated STMS profiling systems
cost ~Rs. 40 /sample/marker
• For 5000 plants – cost is Rs. 2.50 lakhs, but
can be reduced by 1/10th with judicious
bulking procedures.
• Precision & sensitivity is high
• Time scale: 900 plants, 6 days/5000 plants
• No. of persons: 2
20. Seed Enhancement
“Any post harvest treatment that improves
germination / seedling emergence or facilitate
the development of more number of
normal, rapid, uniform and healthy seedlings in
the field conditions.”
McDonald (2000)
21. „Priming of an Idea-Invigoration of Seed‟
Heydecker (1973)
• Prof. Heydecker’s pioneering work on
vegetable seed priming (at Nottingham
University) paved the way for an array of
technologies based on the basic principle
of metabolic advancement of seeds for
enhancing its performance on subsequent
sowing, either immediately or after a
period of storage, and under optimal or
sub-optimal conditions of growing.
23. Magnetic seed treatment
Guoys balance
Sample holder
Gauss meter + Pole pieces
controller
MF probe
Power pack
Indigenously fabricated generator for seed
treatment (IARI, 2010)
24. Yield and growth attributes of maize seed
exposed to electro-magnetic field
Treatment Plant Days Cob Grain
height to 50% weight yield
Control (cm) silking (kg/plot) kg/plot
Treated
Control 155 48 8.7 6.3
Control
100 mT 166 58 9.3 6.8
200 mT 200 mT 162 62 10.5 7.6
Magnetic treatment with 200
mT for 1 hr showed 16%
increase in grain yield than
control
26. Variety Maintenance
• Follow strictly the well defined system of
variety purification and maintenance: an
essential component between the variety
release and seed production
• Identify newer areas for quality seed
production: compact area, seed zone
concept
• In case of licensing insist on replacement
of Breeder seed / Source seed after every
2 – 3 years in case of CP/ SP spp.
27. Quality standards
• Developing quality evaluation protocols,
which are more precise and cost effective
• Revision of protocols and standards
tomeet the needs of emerging
technologies
• Generation of data to rationalise standards
• Generation of database to apply DNAFP
for purity testing
• Generation of database for disease
management
28. Relook into
Seed Certification Standards
1. Rationalise quality
standards
Legal norms to qualify
2. Develop seed quality
standards for • Genetic identity
• Physical purity
• Horticultural spp. (seed
• Germinability
and planting materials)
• Freedom from seed-
• Organic seeds borne pathogens
• Transgenic seeds
29. Capacity Building
• For seed production through contract
farmers : regional agricultural universities
and other institutions can build farmers’
skill and employment.
• Training farmers to upgrade quality of
farm-saved seed which still contributes
~70% of all seed used
30. HRD and infrastructure development
• To have Seed Testing Labs adequately
equipped with facilities and trained
personnel aware of latest technologies
• Establishment of Regional Referral Labs
• Close collaborations of ISTA
• Regular training programmes for seed
analysts ( Specialised Diploma courses )