Biofertilizer developement

1. Production of Enzyme & Biofertilizer from
seafood industry waste (Crustacean Shell)
ARCL ORGANICS LTD
Subrata Parai
Research & Development Executive

2. Contents
• Introduction
• Composition of Waste
• Process mechanism
• Application of Chitinase Enzyme
• Residue composition & application
• Yield percentage
• Market View of Enzyme & Biofertilizer
• Conclusion
• Acknowledgement

3. Introduction
Crustaceans are a very diverse group of invertebrate
animals which includes crabs, lobsters, shrimp, krill
etc. It contains about 11,340,000 species in all habitats.
The exoskeletons of crustaceans made by Chitin, the
most abundant amino polysaccharide polymer
occurring in nature.
 Seafood processing industry produce a large quantity
of crustacean shell waste during processing.
These waste were not recycled properly and having
negative impacts on Environment & Human health.
Fig 1: Some common Crustaceans

4. Introduction
Fermentation of crustacean Shell Waste through
novel microbes leading to the production of enzyme
chitinase & fermentated residue, having important
nutrients .
The enzyme Chitinase applicable in many industry i.e,
Cosmetics, agriculture & pharmaceuticals.
 The Fermented residue having macronutrients usable
as biofertilizer in Agriculture.
By Single fermentation, two commercially valuable
product derived simultaneously from waste
Fig 2: Crustacean Shell waste

5. Composition of waste
The principal components of crustacean
(shrimp, crab, lobster, squilla and krill)
shell waste are:
Chitin (60%)
Protein (20%)
Calcium and Magnesium carbonate
(15%)
Other minor components such as lipid
and minerals (5%)
Fig 3: Crustacean Shell composition
Fig 4: Structure of Chitin

6. {Upstream Processing}
Crustacean Shell Waste
Drying & Grinding
Fermentation
(Chitinolytic Organism)
Fermentated Broth
Solid/Liquid Separation
(Centrifugation/ Filtration)
Liquid
(Enzyme)
Residue
(Fertilizer)
Concentration
(Evaporation/ Adsorption /
Precipitation)
Purification
(IEC»GF »AC)
Packing
Drying
(Desired moisture)
{Downstream Processing}
Note: Fermentation Media
For Enzyme & Biofertilizer
Production Colloidal Chitin
media is required
Composition:
2.0% (w/v) Chitin; NaNO3 0.2%
(w/v); K2HPO4 0.1% (w/v);
KH2PO4 0.1% (w/v); NaCl 0.5%
(w/v); MgSO4•7H2O 0.2% (w/v);
FeCl3 0.01% (w/v); pH 7.0
Process Mechanism

7. Enzyme Structure & Application
Biocontrol
Biopesticide
Cosmetics
Biofertilizer
SCP production
Medicinal use
Chitinase
Chitinases can play a major role in many
areas such as-
Agriculture:
Chitinase can be useful for Fungal pathogen
control & for the production of ophthalmic
products by the use of chitinases and micro
biocides
Medicinal use:
Chitinases has also been observed in
amplifying the functioning of antifungal drugs
in the treatment of fungal diseases. Due to
their applications, Chitinase used in antifungal
creams and lotions.

8. Residue Composition & Fertilizing potentiality
COMPOSITION
1) N-acetylamino
sugar
2) Free amino acids
3) Novel
Microorganism
4) Very less amount
Of Chitinase
Enzyme
Soil Physico-chemical Property

9. Residue Composition & Fertilizing potentiality
Effect on Plant Growth

10. Residue Composition & Fertilizing potentiality
Effect on Rhizosphere

11. Yield Percentage

12. Market View Of Enzyme & Biofertilizer

13. Conclusion

14. Acknowledgement
Special Thanks To:
Dr. Suman Kumar Halder
Assistant Professor, Department Of Microbiology, Vidyasagar University
Mr. Kalyanbrata Pal
P.hd Scholler, Department Of Microbiology, Vidyasagar University
Dr. HareKrishna Jana
Assistant Professor, Department Of Microbiology, Raja N.L.Khan Women's college
Dr. Biplab Kumar Banerjee
Dr. Suresh Chandra Bera
Dr. Asitbaran Mandal
ARCL Organics Ltd