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Slovenian Institute for Hop Research and Brewing




        Certification scheme for the
     production of hop planting material
                 in Slovenia


                                      Vlasta Knapič
                                    Žalec, 7. April 2006




IHPS, Plant protection department
Introduction-1


  New certificate scheme for propagation material and
   hop plants for planting has been prescribed in
   Slovenia in 2003.

  EPPO Standards PM 4/16 determination of plant
   health and quality standards for hop propagation
   material

  Rules of the marketing of hop propagating material
   and of hop plants (SI)

IHPS, Plant protection department
Introduction-2

 Way we have certification scheme for the
  production of hop plant material?



 Main reason is occurrence and spread of
  progressive (lethal) form of hop wilt.




IHPS, Plant protection department
Pre-basic material

 selection of candidate plants (visual plant health checks);
 all candidates test (on viruses and hop viroid)
 white label with violet line

            1. “Rastlinski potni list EU”    2. SI               3. IHPS

            4. Reg. št. dobavitelja: _______________    5. Oznaka partije: __________

            6. Bot. ime: hmelj (Humulus lupulus L.) 7. Količina:
                           IZVORNI RAZMNOŽEVALNI MATERIAL (SE)
            Sorta:     ________________________        Zdrav. status: __________
            Leto pridelave:    20                      Datum izdaje: _____________

            10. Država porekla:                                Ser. št.: 000001


IHPS, Plant protection department
Virus free hop propagating material

                                            Test methods
Virus and viroid                    ELISA    Test plants   Molecular
                                                           techniques
Apple mosaic ilarvirus-              +           +
hop strain (ApMV)
Arabis mosaic nepovirus              +           +
(ArMV)
Hop mosaic carlavirus                +           -
(HMV)
Hop latent carlavirus                +           -
(HLV)
Cherry leaf roll nepovirus           +           +
(CLRV)
Cucumber mosaic                      +           +
cucumovirus (CMV)
Petunia asteroid mosaic              +           +
tombusvirus (PAMV)
Tobacco necrosis virus               +           +
(TNV)
Hop latent viroid (HLVd)              -          -             +


IHPS, Plant protection department
Basic material

 proeither vegetative way from pre-basic material
 retesting on viruses – Apple mosaic ilarvirus (ApMV),
  Arabis mosaic nepovirus (ArMV), Hop mosaic carlavirus
  (HMV)
 white label        1. “Rastlinski potni list EU” 2. SI 3. IHPS

                                    4. Reg. št. dobavitelja: _______________    5. Oznaka partije: __________

                                    6. Bot. ime: hmelj (Humulus lupulus L.) 7. Količina:
                                                   OSNOVNI RAZMNOŽEVALNI MATERIAL (E)
                                    Sorta:    ________________________         Zdrav. status: __________
                                    Leto pridelave:   20                       Datum izdaje: _____________

                                    10. Država porekla:                                Ser. št.: 000001


IHPS, Plant protection department
Certified propagating material A

 certified propagating material A seeds - hop garden
 hop garden must be free hop wild and nematodes
 CPM have blue label
                    1. “Rastlinski potni list EU”    2. SI               3. IHPS

                    4. Reg. št. dobavitelja: _______________    5. Oznaka partije: __________

                    6. Bot. ime: hmelj (Humulus lupulus L.) 7. Količina:
                                         CERTIFICIRANA SADIKA A (CSA)
                    Sorta:     ________________________        Zdrav. status: __________
                    Leto pridelave: 20                         Datum izdaje: _____________

                    10. Država porekla:                                Ser. št.: 000001

IHPS, Plant protection department
Certified cutting B

 Hop garden inspected twice a year
 Retesting 10 % plants in hop garden on Apple
  mosaic ilarvirus (ApMV)
 red label
                                    1. “Rastlinski potni list EU”    2. SI               3. IHPS

                                    4. Reg. št. dobavitelja: _______________    5. Oznaka partije: __________

                                    6. Bot. ime: hmelj (Humulus lupulus L.) 7. Količina:
                                                         CERTIFICIRANE SADIKE B (CSB)
                                    Sorta:     ________________________        Zdrav. status: __________
                                    Leto pridelave: 20                         Datum izdaje: _____________

                                    10. Država porekla:                                Ser. št.: 000001


IHPS, Plant protection department

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Copy of certification hop plants 7.4.06

  • 1. Slovenian Institute for Hop Research and Brewing Certification scheme for the production of hop planting material in Slovenia Vlasta Knapič Žalec, 7. April 2006 IHPS, Plant protection department
  • 2. Introduction-1  New certificate scheme for propagation material and hop plants for planting has been prescribed in Slovenia in 2003.  EPPO Standards PM 4/16 determination of plant health and quality standards for hop propagation material  Rules of the marketing of hop propagating material and of hop plants (SI) IHPS, Plant protection department
  • 3. Introduction-2  Way we have certification scheme for the production of hop plant material?  Main reason is occurrence and spread of progressive (lethal) form of hop wilt. IHPS, Plant protection department
  • 4. Pre-basic material  selection of candidate plants (visual plant health checks);  all candidates test (on viruses and hop viroid)  white label with violet line 1. “Rastlinski potni list EU” 2. SI 3. IHPS 4. Reg. št. dobavitelja: _______________ 5. Oznaka partije: __________ 6. Bot. ime: hmelj (Humulus lupulus L.) 7. Količina: IZVORNI RAZMNOŽEVALNI MATERIAL (SE) Sorta: ________________________ Zdrav. status: __________ Leto pridelave: 20 Datum izdaje: _____________ 10. Država porekla: Ser. št.: 000001 IHPS, Plant protection department
  • 5. Virus free hop propagating material Test methods Virus and viroid ELISA Test plants Molecular techniques Apple mosaic ilarvirus- + + hop strain (ApMV) Arabis mosaic nepovirus + + (ArMV) Hop mosaic carlavirus + - (HMV) Hop latent carlavirus + - (HLV) Cherry leaf roll nepovirus + + (CLRV) Cucumber mosaic + + cucumovirus (CMV) Petunia asteroid mosaic + + tombusvirus (PAMV) Tobacco necrosis virus + + (TNV) Hop latent viroid (HLVd) - - + IHPS, Plant protection department
  • 6. Basic material  proeither vegetative way from pre-basic material  retesting on viruses – Apple mosaic ilarvirus (ApMV), Arabis mosaic nepovirus (ArMV), Hop mosaic carlavirus (HMV)  white label 1. “Rastlinski potni list EU” 2. SI 3. IHPS 4. Reg. št. dobavitelja: _______________ 5. Oznaka partije: __________ 6. Bot. ime: hmelj (Humulus lupulus L.) 7. Količina: OSNOVNI RAZMNOŽEVALNI MATERIAL (E) Sorta: ________________________ Zdrav. status: __________ Leto pridelave: 20 Datum izdaje: _____________ 10. Država porekla: Ser. št.: 000001 IHPS, Plant protection department
  • 7. Certified propagating material A  certified propagating material A seeds - hop garden  hop garden must be free hop wild and nematodes  CPM have blue label 1. “Rastlinski potni list EU” 2. SI 3. IHPS 4. Reg. št. dobavitelja: _______________ 5. Oznaka partije: __________ 6. Bot. ime: hmelj (Humulus lupulus L.) 7. Količina: CERTIFICIRANA SADIKA A (CSA) Sorta: ________________________ Zdrav. status: __________ Leto pridelave: 20 Datum izdaje: _____________ 10. Država porekla: Ser. št.: 000001 IHPS, Plant protection department
  • 8. Certified cutting B  Hop garden inspected twice a year  Retesting 10 % plants in hop garden on Apple mosaic ilarvirus (ApMV)  red label 1. “Rastlinski potni list EU” 2. SI 3. IHPS 4. Reg. št. dobavitelja: _______________ 5. Oznaka partije: __________ 6. Bot. ime: hmelj (Humulus lupulus L.) 7. Količina: CERTIFICIRANE SADIKE B (CSB) Sorta: ________________________ Zdrav. status: __________ Leto pridelave: 20 Datum izdaje: _____________ 10. Država porekla: Ser. št.: 000001 IHPS, Plant protection department

Editor's Notes

  1. I”d like to You introduce Certification s che me for the production of hop planting material in Slovenia. Želela bi vam predstaviti Certifikacijsko shemo sadilnega in razmnoževalnega materiala hmelja v Sloveniji.
  2. Osnova za doseganje velikih in kvalitetnih pridelkov hmelja je sajenje zdravega- brezvirusnega sadilnega materiala v hmeljišča. Smernice za certificiranje razmnoževalnega in sadilnega materiala hmelja so zavedene v EPPO smernicah PM 4/16. Na osnovi tega imamo v Sloveniji Pravilnik o trženju razmnoževalnega materiala in sadik hmelja, ki navaja vse stvari v zvezi s trženjem razmnoževalnega mat. in sadik hmelja. Certifikaciranje opravlja naš Inštitut. If we would like to have a quality and big quantity hop product must seed quality and health material in hop garden. The determination of plant health and quality standards for hop propagating material were set by EPPO standard PM 4/16. EU Member state have different schemes for pathogen tested material of hop. This is unusual situation. Main reason is occurrence and spread of progressive (lethal) form of hop wilt , caused by a new pathotype of Verticillium albo-atrum.
  3. Zelo nenavadno je, da nimajo vse države članice EU vzpostavljene certifikacijske sheme za hmelj. Glavni razlog, da smo se v Sloveniji odločili za certifikacijsko shemo je, da imamo v Sloveniji letalno obliko hmeljeve uvelosti, ki jo povzroča nov patotip glive V. albo-atrum.
  4. kategorija razmnoževalnega materiala hmelja je izvorni razmnoževalni material, ki ga dobimo iz rastlin kandidatk, ki so bile testirane na vse viruse in virusom podobne organizme, ki so pomembne pri hmelju. Če testiranje kandidatk ni negativno, gredo v postopek termo oz. hladno terepijo. Po končanem postopku so rastline potrjene in opremljene z uradnimi etiketami belih barv z vijolično črto, kar je tu prikazano. Uradne etikete vseh stopenj oz. kategorij so ene velikosti z enakimi elementi vsebine, le da je za različne stopnje, različna barva. Izvorni material se običajno hrani v in vitro . The first category of propagation material is pre-basic (nuklear stock) material which has been produced from candidate material
  5. V tabeli so navedeni vsi virusi in hmeljev viruid, na katere testiramo izvorni razmnoževalni material. V prvi vrsti se testira z ELISA testom ,ki omogoča v kratkem času veliko število dokaj poceni opraviti.
  6. The second category of propagation material is basic (propagation) material which has been produced directly orsec 2. kategorija razmnoževalnega materiala hmelja je osnovni razmnoževalni material, katerega vegetativno pridobimo iz izvornega razmnoževalnega materiala. Osnovni razmnoževalni material retestiramo in sicer ne na vse viruse, temveč le na tri, ki so pomembni pri količini in kvaliteti hmelja: Apple mosaic ilar virus, Arabis mosaic nepovirus , Hop mosaic carlavirus.
  7. 3. Kategorija razmnoževalnega materiala hmelja je certificiran razmnoževalni material A, namenjen za sajenje v hmeljišča.
  8. Matično hmeljišče mora biti prijavljeno in potem 2-krat letno opravimo zdravstveni pregled in pregled na sortno čistost. Testirano je vsako leto in sicer 10 % rastlin na jablanov mosaic virus. Ko je vse opravljeno je nasad potrjen in pridobi certifikat o uradni potrditvi. Hmeljar reži iz nasada sadike, ki jih da v pregled. Pregledamo 3 % sadik, ki jih opremimo z uradnimi etiketami rdeče barve.